##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063887_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8699
Sequences flagged as poor quality	0
Sequence length	25
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.24508564202782	32.0	32.0	32.0	32.0	32.0
2	30.923439475801818	32.0	32.0	32.0	32.0	32.0
3	30.893436027129557	32.0	32.0	32.0	32.0	32.0
4	30.909070008046903	32.0	32.0	32.0	32.0	32.0
5	30.837682492240486	32.0	32.0	32.0	32.0	32.0
6	34.37418094033797	36.0	36.0	36.0	32.0	36.0
7	34.42901482929072	36.0	36.0	36.0	32.0	36.0
8	34.30359811472583	36.0	36.0	36.0	32.0	36.0
9	34.458328543510746	36.0	36.0	36.0	32.0	36.0
10	34.20255201747327	36.0	36.0	36.0	32.0	36.0
11	34.39441315093689	36.0	36.0	36.0	32.0	36.0
12	34.30244855730544	36.0	36.0	36.0	32.0	36.0
13	34.338084837337625	36.0	36.0	36.0	32.0	36.0
14	34.215427060581675	36.0	36.0	36.0	32.0	36.0
15	34.18289458558455	36.0	36.0	36.0	32.0	36.0
16	34.21669157374411	36.0	36.0	36.0	32.0	36.0
17	34.10978273364755	36.0	36.0	36.0	32.0	36.0
18	34.10552937119209	36.0	36.0	36.0	32.0	36.0
19	34.092424416599606	36.0	36.0	36.0	32.0	36.0
20	34.17013449821819	36.0	36.0	36.0	32.0	36.0
21	34.1005862742844	36.0	36.0	36.0	32.0	36.0
22	34.22002529026325	36.0	36.0	36.0	32.0	36.0
23	33.98563053224508	36.0	36.0	36.0	32.0	36.0
24	34.12334751120819	36.0	36.0	36.0	32.0	36.0
25	33.78951603632601	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
5	2.0
6	2.0
7	0.0
8	0.0
9	1.0
10	1.0
11	0.0
12	0.0
13	1.0
14	0.0
15	4.0
16	2.0
17	7.0
18	6.0
19	6.0
20	13.0
21	19.0
22	15.0
23	37.0
24	38.0
25	76.0
26	99.0
27	125.0
28	179.0
29	247.0
30	303.0
31	374.0
32	530.0
33	809.0
34	1682.0
35	4121.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	44.0036794296884	19.443486259629758	11.233758767391054	25.319075543290793
2	20.404644211978386	19.588458443499253	34.854580986320265	25.152316358202093
3	21.324290148292906	23.324520059776983	27.577882515231632	27.77330727669847
4	16.879383695527192	17.569276762101875	34.01172818213177	31.53961136023916
5	18.376264949402024	33.45216191352346	32.17571297148114	15.995860165593376
6	33.72052903967798	34.37607820586544	17.88384128809661	14.019551466359978
7	31.167337550316276	27.901092581943647	21.11558366877516	19.81598619896492
8	27.488206190311814	31.69945921067771	19.710044874007593	21.102289725002876
9	26.823935558112773	15.063291139240507	19.631760644418872	38.48101265822785
10	18.95063859164653	24.289494879760674	29.593832700494765	27.166033828098037
11	37.47986191024166	21.242807825086306	22.704257767548906	18.573072497123132
12	25.762633820651548	22.562449637389204	27.351214458386092	24.323702083573156
13	31.385181776346066	19.822825586746433	25.12655315232398	23.665439484583526
14	25.561054206467947	18.460121993324893	23.765680745770513	32.21314305443664
15	27.974683544303797	25.765247410817032	21.518987341772153	24.74108170310702
16	27.15551974214343	26.787153217451365	21.92931967307471	24.128007367330497
17	25.587016574585636	25.483425414364643	24.505064456721914	24.42449355432781
18	26.15402325313687	25.693565097271787	24.438816622539427	23.713595027051916
19	27.15551974214343	25.187061125820193	23.794175204328308	23.86324392770807
20	27.104202648244097	25.15831894070236	24.82440990213011	22.91306850892343
21	27.676721160488142	24.10775961317062	23.370941745337323	24.844577481003917
22	26.988145931637703	23.961330417769595	24.67487628035447	24.375647370238234
23	26.277624309392266	24.021639042357272	25.241712707182316	24.45902394106814
24	26.910681399631674	24.090699815837937	24.447513812154696	24.55110497237569
25	26.533901231725565	23.828709566018187	24.979854955680903	24.65753424657534
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	2.5
7	5.0
8	5.0
9	5.0
10	12.5
11	20.0
12	20.0
13	20.0
14	24.0
15	28.0
16	28.0
17	28.0
18	65.5
19	103.0
20	103.0
21	103.0
22	140.5
23	178.0
24	178.0
25	178.0
26	238.0
27	298.0
28	298.0
29	298.0
30	400.0
31	502.0
32	502.0
33	502.0
34	559.5
35	617.0
36	617.0
37	617.0
38	699.0
39	781.0
40	781.0
41	781.0
42	791.5
43	802.0
44	802.0
45	802.0
46	815.0
47	828.0
48	828.0
49	828.0
50	866.5
51	905.0
52	905.0
53	905.0
54	874.5
55	844.0
56	844.0
57	844.0
58	796.5
59	749.0
60	749.0
61	749.0
62	692.0
63	635.0
64	635.0
65	635.0
66	564.0
67	493.0
68	493.0
69	493.0
70	415.5
71	338.0
72	338.0
73	338.0
74	267.0
75	196.0
76	196.0
77	196.0
78	148.0
79	100.0
80	100.0
81	100.0
82	73.0
83	46.0
84	46.0
85	46.0
86	30.5
87	15.0
88	15.0
89	15.0
90	13.5
91	12.0
92	12.0
93	12.0
94	8.5
95	5.0
96	5.0
97	5.0
98	102.0
99	199.0
100	199.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.022991148407862972
2	0.0
3	0.0
4	0.022991148407862972
5	0.03448672261179446
6	0.045982296815725944
7	0.045982296815725944
8	0.09196459363145189
9	0.10346016783538338
10	0.09196459363145189
11	0.10346016783538338
12	0.13794689044717784
13	0.08046901942752041
14	0.11495574203931487
15	0.10346016783538338
16	0.13794689044717784
17	0.12645131624324635
18	0.13794689044717784
19	0.13794689044717784
20	0.16093803885504082
21	0.14944246465110933
22	0.11495574203931487
23	0.12645131624324635
24	0.12645131624324635
25	0.13794689044717784
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	8699.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	93.52799172318657
#Duplication Level	Percentage of deduplicated	Percentage of total
1	95.56293018682399	89.3780894355673
2	4.240412979351032	7.931946200712725
3	0.18436578171091444	0.5173008391769169
4	0.0	0.0
5	0.0	0.0
6	0.0	0.0
7	0.0	0.0
8	0.0	0.0
9	0.0	0.0
>10	0.0	0.0
>50	0.0	0.0
>100	0.012291052114060964	2.1726635245430512
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGGGGGGGGGGGGGGGGGGGGGGGG	189	2.1726635245430512	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE