FastQCFastQC Report
Thu 2 Feb 2017
SRR4063887_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063887_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8699
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGT320.36785837452580755No Hit
ATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTT130.14944246465110933TruSeq Adapter, Index 12 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATGTCGTATGCCGT100.11495574203931487No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCCAC250.002226409535.217
ATCTCCG250.002226409535.210
GCCCACG250.002226409535.218
CACGAGA250.002226409535.221
CCCACGA250.002226409535.219
TAGTAAT250.002226409535.232
GACGCGT250.002226409535.226
ACGAGAC250.002226409535.222
AGACGCG250.002226409535.225
CGAGCCC250.002226409535.215
TCTCCGA250.002226409535.211
GCGTAGT250.002226409535.229
CGAGACG250.002226409535.223
TCCGAGC250.002226409535.213
CGCGTAG250.002226409535.228
GAGACGC250.002226409535.224
CTCCGAG250.002226409535.212
CCACGAG300.00543928829.33333220
CGTAGTA300.00543928829.33333230
GAGCCCA300.00543928829.33333216
Produced by FastQC (version 0.11.5)