##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063887_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8699
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.32072652028969	32.0	32.0	32.0	32.0	32.0
2	31.473847568686057	32.0	32.0	32.0	32.0	32.0
3	31.445338544660306	32.0	32.0	32.0	32.0	32.0
4	31.543165881135764	32.0	32.0	32.0	32.0	32.0
5	31.485573054374065	32.0	32.0	32.0	32.0	32.0
6	35.05494884469479	36.0	36.0	36.0	36.0	36.0
7	35.10541441545005	36.0	36.0	36.0	36.0	36.0
8	35.00436831819749	36.0	36.0	36.0	36.0	36.0
9	35.126336360501206	36.0	36.0	36.0	36.0	36.0
10	34.96735256926083	36.0	36.0	36.0	36.0	36.0
11	35.146453615358084	36.0	36.0	36.0	36.0	36.0
12	35.00942637084722	36.0	36.0	36.0	36.0	36.0
13	35.02839406828371	36.0	36.0	36.0	36.0	36.0
14	34.97781354178641	36.0	36.0	36.0	36.0	36.0
15	34.884929302218644	36.0	36.0	36.0	32.0	36.0
16	34.942177261754225	36.0	36.0	36.0	36.0	36.0
17	34.91079434417749	36.0	36.0	36.0	36.0	36.0
18	34.8961949649385	36.0	36.0	36.0	32.0	36.0
19	34.93746407633061	36.0	36.0	36.0	32.0	36.0
20	34.90079319462007	36.0	36.0	36.0	32.0	36.0
21	34.918381423152084	36.0	36.0	36.0	32.0	36.0
22	34.852971605931714	36.0	36.0	36.0	32.0	36.0
23	34.849178066444416	36.0	36.0	36.0	32.0	36.0
24	34.831704793654445	36.0	36.0	36.0	32.0	36.0
25	34.779055063800435	36.0	36.0	36.0	32.0	36.0
26	34.73111851937004	36.0	36.0	36.0	32.0	36.0
27	34.61455339694218	36.0	36.0	36.0	32.0	36.0
28	34.66708817105415	36.0	36.0	36.0	32.0	36.0
29	34.710656397287046	36.0	36.0	36.0	32.0	36.0
30	34.575928267616966	36.0	36.0	36.0	32.0	36.0
31	34.653293482009424	36.0	36.0	36.0	32.0	36.0
32	34.55017818140016	36.0	36.0	36.0	32.0	36.0
33	34.51017358317048	36.0	36.0	36.0	32.0	36.0
34	34.44120013794689	36.0	36.0	36.0	32.0	36.0
35	34.50063225658121	36.0	36.0	36.0	32.0	36.0
36	34.33233705023566	36.0	36.0	36.0	32.0	36.0
37	34.32509483848718	36.0	36.0	36.0	32.0	36.0
38	34.17473272789976	36.0	36.0	36.0	32.0	36.0
39	34.14783308426256	36.0	36.0	36.0	32.0	36.0
40	34.14783308426256	36.0	36.0	36.0	32.0	36.0
41	34.14220025290263	36.0	36.0	36.0	32.0	36.0
42	34.27750316128291	36.0	36.0	36.0	32.0	36.0
43	34.10575928267617	36.0	36.0	36.0	32.0	36.0
44	34.076905391424305	36.0	36.0	36.0	32.0	36.0
45	34.10909299919531	36.0	36.0	36.0	32.0	36.0
46	34.06184618921715	36.0	36.0	36.0	32.0	36.0
47	34.02218645821359	36.0	36.0	36.0	32.0	36.0
48	34.178526267387056	36.0	36.0	36.0	32.0	36.0
49	33.93849867800897	36.0	36.0	36.0	32.0	36.0
50	33.24025750086217	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	1.0
21	0.0
22	1.0
23	7.0
24	17.0
25	26.0
26	56.0
27	98.0
28	135.0
29	192.0
30	248.0
31	332.0
32	472.0
33	662.0
34	1315.0
35	5137.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	40.699011266957925	21.326741779719477	11.623361692343067	26.350885260979535
2	17.404299344752268	21.30129899988504	36.31451891021957	24.97988274514312
3	18.358432003678583	23.922289918381423	30.095413265892628	27.623864812047362
4	14.093573974020002	19.806874353373953	34.18783768249224	31.911713990113803
5	15.174157949189562	35.72824462581906	32.739395332796875	16.358202092194503
6	31.26796183469364	35.394872973905045	18.025060351764573	15.312104839636739
7	28.15266122542821	28.819404529256236	21.864582135877686	21.163352109437866
8	27.22151971490976	31.762271525462697	20.3356707667548	20.680537992872743
9	24.54305092539372	15.335095988044603	20.19772387630762	39.92412921025405
10	17.197379009081505	25.623634900563285	29.658581446143234	27.520404644211975
11	35.90067823887803	22.485343142889988	22.956661685251177	18.6573169329808
12	23.97976778940108	22.818714794804002	27.761811702494537	25.43970571330038
13	30.15289113691229	19.50798942407173	25.16381193240602	25.175307506609958
14	22.910679388435454	19.439015978848143	24.048741234624668	33.601563398091734
15	27.095068398666513	26.014484423496953	20.128750431084033	26.7616967467525
16	26.79926419866636	25.856518739940213	23.246723384686135	24.097493676707288
17	23.462466950224165	25.830555236234048	26.23290033337165	24.474077480170134
18	26.61225428210139	24.163696976663985	24.66950224163697	24.554546499597656
19	25.715599494194734	24.497068628578	23.922289918381423	25.865041958845847
20	24.405104034946547	24.956891596735257	24.35912173813082	26.27888263018738
21	26.244395907575583	24.382112886538682	23.152086446718016	26.221404759167722
22	24.35912173813082	26.68122772732498	24.209679273479708	24.74997126106449
23	25.796068513622256	24.267157144499368	24.209679273479708	25.727095068398665
24	26.083457868720544	24.692493390044834	23.8418208989539	25.382227842280724
25	25.43113359392964	25.534605656472753	23.591630259829845	25.442630489767765
26	26.307922272047833	25.1925951477521	24.23824307232379	24.26123950787628
27	24.747010119595217	25.74747010119595	24.25252989880405	25.252989880404787
28	24.65501379944802	24.89650413983441	24.781508739650416	25.666973321067154
29	26.146947223180405	24.146257330113833	24.744164654478556	24.962630792227205
30	24.353225250086236	24.6521789122686	25.34207197884328	25.652523858801885
31	26.143941135893307	24.37341917682226	24.281444010117266	25.201195677167163
32	23.83032532474997	24.451086331762273	25.77307736521439	25.94551097827337
33	24.405104034946547	24.933900448327396	25.681112771582942	24.97988274514312
34	26.534835594389516	23.718096114049207	24.097493676707288	25.649574614853986
35	24.772962409472356	25.520174732727902	26.405333946430627	23.301528911369125
36	25.402483900643976	25.034498620055196	23.94204231830727	25.62097516099356
37	25.18969878132904	25.982984594159575	24.338928489307886	24.488388135203497
38	24.574517019319227	23.838546458141675	26.21895124195032	25.36798528058878
39	25.10060940554214	24.28423594342877	25.549039898815685	25.06611475221341
40	24.58903322220945	24.2786527187033	26.30187377859524	24.83044028049201
41	24.692493390044834	24.577537648005517	24.81894470628808	25.91102425566157
42	24.867785697861578	23.833065072430443	25.87951253161646	25.419636698091512
43	24.876422577307736	22.956661685251177	26.554776411081733	25.61213932635935
44	24.959760864566565	24.948263968728444	25.040239135433435	25.051736031271552
45	24.281444010117266	23.177742009657393	26.270406990112672	26.270406990112672
46	25.546102552310877	22.60289721775121	25.77604046907335	26.07495976086457
47	23.876307621565697	23.807334176342106	26.738705598344637	25.57765260374756
48	23.359006782388782	23.623404989079205	27.04908610185079	25.968502126681226
49	24.15220140246005	23.416484653408435	26.497298540062076	25.934015404069434
50	24.01701540584042	23.465164405610487	26.98321453207634	25.534605656472753
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.5
4	1.0
5	2.0
6	3.0
7	2.0
8	1.0
9	0.5
10	0.0
11	0.0
12	0.0
13	0.5
14	1.0
15	1.5
16	2.0
17	3.5
18	5.0
19	8.5
20	12.0
21	23.5
22	35.0
23	42.0
24	49.0
25	59.0
26	69.0
27	89.5
28	110.0
29	140.0
30	170.0
31	198.0
32	226.0
33	251.5
34	277.0
35	312.0
36	347.0
37	380.0
38	413.0
39	417.5
40	422.0
41	463.5
42	505.0
43	500.5
44	496.0
45	478.5
46	461.0
47	482.0
48	503.0
49	473.5
50	444.0
51	478.5
52	513.0
53	506.5
54	500.0
55	458.5
56	417.0
57	412.0
58	407.0
59	402.0
60	397.0
61	410.5
62	424.0
63	379.5
64	335.0
65	322.5
66	310.0
67	274.0
68	238.0
69	211.0
70	184.0
71	161.5
72	139.0
73	120.0
74	101.0
75	86.5
76	72.0
77	61.5
78	51.0
79	40.0
80	29.0
81	23.0
82	17.0
83	11.5
84	6.0
85	5.0
86	4.0
87	2.5
88	1.0
89	0.5
90	0.0
91	0.0
92	0.0
93	1.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.011495574203931486
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.011495574203931486
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.011495574203931486
26	0.022991148407862972
27	0.03448672261179446
28	0.03448672261179446
29	0.022991148407862972
30	0.022991148407862972
31	0.011495574203931486
32	0.0
33	0.0
34	0.011495574203931486
35	0.0
36	0.03448672261179446
37	0.011495574203931486
38	0.03448672261179446
39	0.022991148407862972
40	0.0
41	0.0
42	0.011495574203931486
43	0.0
44	0.011495574203931486
45	0.011495574203931486
46	0.011495574203931486
47	0.0
48	0.0
49	0.0
50	0.011495574203931486
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	8699.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	94.64306242096792
#Duplication Level	Percentage of deduplicated	Percentage of total
1	95.39657476011175	90.2862397976779
2	4.299769221425969	8.138866536383492
3	0.194339851815863	0.5517875617887114
4	0.024292481476982875	0.09196459363145189
5	0.012146240738491437	0.057477871019657435
6	0.012146240738491437	0.06897344522358892
7	0.012146240738491437	0.08046901942752041
8	0.012146240738491437	0.09196459363145189
9	0.0	0.0
>10	0.03643872221547431	0.6322565812162317
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGT	32	0.36785837452580755	No Hit
ATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTT	13	0.14944246465110933	TruSeq Adapter, Index 12 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATGTCGTATGCCGT	10	0.11495574203931487	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	0.0	0.0
26	0.0	0.0	0.0	0.0	0.0
27	0.0	0.0	0.0	0.022991148407862972	0.0
28	0.0	0.0	0.0	0.045982296815725944	0.0
29	0.0	0.0	0.0	0.10346016783538338	0.0
30	0.0	0.0	0.0	0.16093803885504082	0.0
31	0.0	0.0	0.0	0.21841590987469825	0.0
32	0.0	0.0	0.0	0.425336245545465	0.0
33	0.0	0.0	0.0	0.5977698586044373	0.0
34	0.0	0.0	0.0	0.7242211748476837	0.0
35	0.0	0.0	0.0	0.919645936314519	0.0
36	0.0	0.0	0.0	1.1265662719852856	0.0
37	0.0	0.0	0.0	1.5059202207150246	0.0
38	0.0	0.0	0.0	1.8967697436486952	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGCCCAC	25	0.0022264095	35.2	17
ATCTCCG	25	0.0022264095	35.2	10
GCCCACG	25	0.0022264095	35.2	18
CACGAGA	25	0.0022264095	35.2	21
CCCACGA	25	0.0022264095	35.2	19
TAGTAAT	25	0.0022264095	35.2	32
GACGCGT	25	0.0022264095	35.2	26
ACGAGAC	25	0.0022264095	35.2	22
AGACGCG	25	0.0022264095	35.2	25
CGAGCCC	25	0.0022264095	35.2	15
TCTCCGA	25	0.0022264095	35.2	11
GCGTAGT	25	0.0022264095	35.2	29
CGAGACG	25	0.0022264095	35.2	23
TCCGAGC	25	0.0022264095	35.2	13
CGCGTAG	25	0.0022264095	35.2	28
GAGACGC	25	0.0022264095	35.2	24
CTCCGAG	25	0.0022264095	35.2	12
CCACGAG	30	0.005439288	29.333332	20
CGTAGTA	30	0.005439288	29.333332	30
GAGCCCA	30	0.005439288	29.333332	16
>>END_MODULE