FastQCFastQC Report
Thu 2 Feb 2017
SRR4063886_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063886_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2583587
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT42640.16504185847041342No Hit
TATCAACGCAGAGTACTTTTTTTTT26790.10369304381853602No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGCTA954.8930815E-914.00279816
CGCATCG2600.013.15520113
AACCGCG1750.012.4850657
TCGCGTA853.9424813E-612.2951469
GTATCAA30900.012.172711
ACGCGCG1201.0009899E-811.87622313
CGCGGTC1657.2759576E-1211.51656110
ATCGCCA2900.011.46780816
CGTCTTA1354.7202775E-911.26129115
GTATTAG3000.011.081471
GGTTCTA4300.011.04764913
CGCGTAA951.3592051E-511.00134710
CGTCCAA1053.4634286E-610.8584710
CCGATCA700.001490941210.858059
GTGTTAG1701.4006218E-1010.6158621
GCATATA1909.094947E-1210.4982341
ATACCGT3800.010.49782856
CGCGCGC1451.4406396E-810.4846514
CGACCCG2450.010.4666145
GCGTTAT1659.331416E-1010.3618941
Produced by FastQC (version 0.11.5)