##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063886_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2583587
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.217312209730114	32.0	32.0	32.0	32.0	32.0
2	30.68354810579245	32.0	32.0	32.0	32.0	32.0
3	30.713155779155105	32.0	32.0	32.0	32.0	32.0
4	30.758402562019395	32.0	32.0	32.0	32.0	32.0
5	30.59167699791027	32.0	32.0	32.0	32.0	32.0
6	34.2947843444018	36.0	36.0	36.0	32.0	36.0
7	34.228919715109264	36.0	36.0	36.0	32.0	36.0
8	34.171422909311744	36.0	36.0	36.0	32.0	36.0
9	34.37652922080812	36.0	36.0	36.0	32.0	36.0
10	33.96280287832382	36.0	36.0	36.0	32.0	36.0
11	34.36201993584888	36.0	36.0	36.0	32.0	36.0
12	34.11963792974651	36.0	36.0	36.0	32.0	36.0
13	34.231330704172144	36.0	36.0	36.0	32.0	36.0
14	34.1041021649358	36.0	36.0	36.0	32.0	36.0
15	34.01901697136578	36.0	36.0	36.0	32.0	36.0
16	34.025810626853286	36.0	36.0	36.0	32.0	36.0
17	33.9353124164195	36.0	36.0	36.0	32.0	36.0
18	33.932259296861304	36.0	36.0	36.0	32.0	36.0
19	33.962327570157306	36.0	36.0	36.0	32.0	36.0
20	33.943271505856	36.0	36.0	36.0	32.0	36.0
21	33.93605169866546	36.0	36.0	36.0	32.0	36.0
22	33.90504171138808	36.0	36.0	36.0	32.0	36.0
23	33.82890454240558	36.0	36.0	36.0	32.0	36.0
24	33.82301931384544	36.0	36.0	36.0	32.0	36.0
25	33.371770333261466	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	7.0
4	17.0
5	26.0
6	100.0
7	32.0
8	127.0
9	163.0
10	241.0
11	85.0
12	165.0
13	114.0
14	261.0
15	415.0
16	748.0
17	966.0
18	1377.0
19	1921.0
20	2894.0
21	4622.0
22	7223.0
23	11006.0
24	16331.0
25	23514.0
26	33101.0
27	43911.0
28	58076.0
29	77290.0
30	101563.0
31	136741.0
32	190004.0
33	264009.0
34	541776.0
35	1064761.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.64499029260046	16.837982068996492	11.32563504669387	26.191392591709178
2	16.863851386266962	19.487553266969854	37.36373352057657	26.284861826186624
3	18.94083722010412	23.88310657815796	28.031429622031702	29.144626579706216
4	12.90790062949414	15.500969033130598	35.44474304848882	36.146387288886444
5	14.94288646233611	36.425930076415895	33.403087370996644	15.22809609025135
6	34.487024793740346	34.805380615985996	17.114412607571865	13.593181982701791
7	30.53194112848808	30.044222931891728	20.85473407589248	18.56910186372771
8	28.423112485035084	32.63098267399954	19.2361273516015	19.709777489363876
9	27.727823317657702	14.307848039306803	18.038710446910414	39.925618196125086
10	15.940259317780464	26.717709204696842	31.516017597670164	25.82601387985253
11	37.082664010546	20.839032581368222	22.638536262708097	19.439767145377683
12	25.385189184415978	23.068360768372152	28.63266069656674	22.913789350645125
13	29.809090324614612	19.484506695316803	24.86779392661527	25.838609053453315
14	23.96892352847082	19.191723579131402	25.0491791087843	31.79017378361347
15	25.440794143993596	26.789385852838443	21.955454105009835	25.814365898158126
16	25.976598810187767	25.31910361281527	23.392088058499102	25.312209518497863
17	24.536191795953048	25.453622277975857	24.869887381612816	25.140298544458272
18	25.153518243425143	24.73932015424453	25.52750894304534	24.57965265928498
19	26.023954141542553	24.57075481381719	24.810443420048085	24.594847624592177
20	26.194524971219536	24.191203015790133	24.48671605183118	25.127555961159153
21	26.821745361583453	23.944765077274663	24.08506023163032	25.14842932951156
22	26.204778675227647	24.10653554567724	24.297693400618833	25.390992378476284
23	24.83659801581783	23.979117704822396	24.969775523461593	26.21450875589818
24	25.29724784288085	24.54510311873955	24.66386281202502	25.49378622635458
25	25.330839288259753	24.16490291352393	24.846220351903614	25.658037446312697
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	147.0
1	147.0
2	380.0
3	613.0
4	613.0
5	613.0
6	1649.5
7	2686.0
8	2686.0
9	2686.0
10	2920.5
11	3155.0
12	3155.0
13	3155.0
14	4115.5
15	5076.0
16	5076.0
17	5076.0
18	8811.5
19	12547.0
20	12547.0
21	12547.0
22	21060.5
23	29574.0
24	29574.0
25	29574.0
26	45749.5
27	61925.0
28	61925.0
29	61925.0
30	80579.0
31	99233.0
32	99233.0
33	99233.0
34	125788.0
35	152343.0
36	152343.0
37	152343.0
38	179592.0
39	206841.0
40	206841.0
41	206841.0
42	238485.5
43	270130.0
44	270130.0
45	270130.0
46	304618.0
47	339106.0
48	339106.0
49	339106.0
50	349521.5
51	359937.0
52	359937.0
53	359937.0
54	341449.5
55	322962.0
56	322962.0
57	322962.0
58	295980.0
59	268998.0
60	268998.0
61	268998.0
62	234282.5
63	199567.0
64	199567.0
65	199567.0
66	161908.5
67	124250.0
68	124250.0
69	124250.0
70	93258.0
71	62266.0
72	62266.0
73	62266.0
74	46673.0
75	31080.0
76	31080.0
77	31080.0
78	24292.5
79	17505.0
80	17505.0
81	17505.0
82	12117.0
83	6729.0
84	6729.0
85	6729.0
86	5043.0
87	3357.0
88	3357.0
89	3357.0
90	2446.5
91	1536.0
92	1536.0
93	1536.0
94	1086.0
95	636.0
96	636.0
97	636.0
98	1012.0
99	1388.0
100	1388.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03951870016376457
2	0.005341410991772292
3	0.0014321174398230058
4	0.0032512936471657426
5	0.00867011639244198
6	0.012153645300119562
7	0.020823761692561546
8	0.03402246566498438
9	0.04780175778868682
10	0.05523328612506565
11	0.05322058053396305
12	0.06227775569392477
13	0.06320669673597212
14	0.06572257872485036
15	0.05751693285343207
16	0.06452269654553922
17	0.06165846166589319
18	0.07632798895489101
19	0.07369598933575684
20	0.07644410658514693
21	0.0730379894309733
22	0.07551516554309957
23	0.08012116487658438
24	0.07365728345900487
25	0.07628928307813904
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2583587.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.397743221203314
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.13431222934493	37.865997748362425
2	13.634299440171175	13.742758443734862
3	4.557416239229059	6.890504801304247
4	2.03062748505607	4.093561702790947
5	1.1639368498215577	2.932989524150158
6	0.7461327287843587	2.2562043404525904
7	0.47466989048205055	1.674560387874576
8	0.35483132500531017	1.4306158403565572
9	0.26267643880131136	1.1914469741671758
>10	1.4737127554507592	13.366006895814142
>50	0.09280781751454101	3.2451831533047675
>100	0.06446392019445506	6.699907472291759
>500	0.007024977810253682	2.3933308680345946
>1k	0.0030879023341755206	2.21693184736135
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	4264	0.16504185847041342	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2679	0.10369304381853602	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	3.870587675197313E-5	0.0
5	0.0	0.0	0.0	3.870587675197313E-5	0.0
6	0.0	0.0	0.0	3.870587675197313E-5	0.0
7	0.0	0.0	0.0	3.870587675197313E-5	0.0
8	0.0	0.0	0.0	3.870587675197313E-5	0.0
9	0.0	0.0	0.0	3.870587675197313E-5	0.0
10	0.0	0.0	0.0	1.5482350700789252E-4	0.0
11	0.0	0.0	0.0	1.5482350700789252E-4	0.0
12	0.0	0.0	0.0	1.5482350700789252E-4	1.9352938375986564E-4
13	0.0	0.0	0.0	1.5482350700789252E-4	1.9352938375986564E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGCTA	95	4.8930815E-9	14.002798	16
CGCATCG	260	0.0	13.155201	13
AACCGCG	175	0.0	12.485065	7
TCGCGTA	85	3.9424813E-6	12.295146	9
GTATCAA	3090	0.0	12.17271	1
ACGCGCG	120	1.0009899E-8	11.876223	13
CGCGGTC	165	7.2759576E-12	11.516561	10
ATCGCCA	290	0.0	11.467808	16
CGTCTTA	135	4.7202775E-9	11.261291	15
GTATTAG	300	0.0	11.08147	1
GGTTCTA	430	0.0	11.047649	13
CGCGTAA	95	1.3592051E-5	11.001347	10
CGTCCAA	105	3.4634286E-6	10.85847	10
CCGATCA	70	0.0014909412	10.85805	9
GTGTTAG	170	1.4006218E-10	10.615862	1
GCATATA	190	9.094947E-12	10.498234	1
ATACCGT	380	0.0	10.4978285	6
CGCGCGC	145	1.4406396E-8	10.48465	14
CGACCCG	245	0.0	10.466614	5
GCGTTAT	165	9.331416E-10	10.361894	1
>>END_MODULE