FastQCFastQC Report
Thu 2 Feb 2017
SRR4063886_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063886_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2583587
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA34400.024.557991
AACGCAG46500.018.4038336
TCAACGC48450.017.663124
CAACGCA49750.017.201575
TATCAAC49900.017.0613572
ATCAACG50250.017.030413
ATACCGT5200.016.499586
TAATACC3500.016.342444
TAGGACG5000.016.2795854
GGTATCA16600.015.6384571
CGACGGT5100.015.5290157
TACGGGT1158.2250925E-515.3039584
ACCGTCG4750.015.2838228
ACGCAGA56050.015.2681217
GTACATG80100.015.1609541
TTAACGG1603.8277358E-715.12519935
AGAGTAC57750.014.819242511
ACGGTAT5350.014.8036489
TACGACG5500.014.79962355
ACATGGG79550.014.7953493
Produced by FastQC (version 0.11.5)