##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063886_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2583587
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.320405699517764	32.0	32.0	32.0	32.0	32.0
2	31.382808862252364	32.0	32.0	32.0	32.0	32.0
3	31.478903942464488	32.0	32.0	32.0	32.0	32.0
4	31.57016969043427	32.0	32.0	32.0	32.0	32.0
5	31.51390257034116	32.0	32.0	32.0	32.0	32.0
6	35.11895670631568	36.0	36.0	36.0	36.0	36.0
7	35.133936654736225	36.0	36.0	36.0	36.0	36.0
8	35.08575596641413	36.0	36.0	36.0	36.0	36.0
9	35.18937508200808	36.0	36.0	36.0	36.0	36.0
10	35.049840396317215	36.0	36.0	36.0	36.0	36.0
11	35.19246381097288	36.0	36.0	36.0	36.0	36.0
12	35.10210571581294	36.0	36.0	36.0	36.0	36.0
13	35.14268611817601	36.0	36.0	36.0	36.0	36.0
14	35.089122603574026	36.0	36.0	36.0	36.0	36.0
15	35.0567915847231	36.0	36.0	36.0	36.0	36.0
16	35.073169202353164	36.0	36.0	36.0	36.0	36.0
17	35.04004122949991	36.0	36.0	36.0	36.0	36.0
18	35.037056232284804	36.0	36.0	36.0	36.0	36.0
19	35.03727375931215	36.0	36.0	36.0	36.0	36.0
20	35.02872014760873	36.0	36.0	36.0	36.0	36.0
21	35.021811535667275	36.0	36.0	36.0	36.0	36.0
22	35.005612352129035	36.0	36.0	36.0	36.0	36.0
23	34.954200884274464	36.0	36.0	36.0	36.0	36.0
24	34.928744029134684	36.0	36.0	36.0	36.0	36.0
25	34.90351940925542	36.0	36.0	36.0	32.0	36.0
26	34.84358839086897	36.0	36.0	36.0	32.0	36.0
27	34.83191353726428	36.0	36.0	36.0	32.0	36.0
28	34.81233455656806	36.0	36.0	36.0	32.0	36.0
29	34.792387095925164	36.0	36.0	36.0	32.0	36.0
30	34.77079502257907	36.0	36.0	36.0	32.0	36.0
31	34.764864121084365	36.0	36.0	36.0	32.0	36.0
32	34.72701480538492	36.0	36.0	36.0	32.0	36.0
33	34.70649024011965	36.0	36.0	36.0	32.0	36.0
34	34.69736649085167	36.0	36.0	36.0	32.0	36.0
35	34.66252539589338	36.0	36.0	36.0	32.0	36.0
36	34.63462310346042	36.0	36.0	36.0	32.0	36.0
37	34.630932498112124	36.0	36.0	36.0	32.0	36.0
38	34.59168435202685	36.0	36.0	36.0	32.0	36.0
39	34.59360145410238	36.0	36.0	36.0	32.0	36.0
40	34.571870039599986	36.0	36.0	36.0	32.0	36.0
41	34.558462323893096	36.0	36.0	36.0	32.0	36.0
42	34.5017017038714	36.0	36.0	36.0	32.0	36.0
43	34.49348522035449	36.0	36.0	36.0	32.0	36.0
44	34.44238301245517	36.0	36.0	36.0	32.0	36.0
45	34.414315058869704	36.0	36.0	36.0	32.0	36.0
46	34.40242732294287	36.0	36.0	36.0	32.0	36.0
47	34.36783239736072	36.0	36.0	36.0	32.0	36.0
48	34.33917224386096	36.0	36.0	36.0	32.0	36.0
49	34.32680029741596	36.0	36.0	36.0	32.0	36.0
50	33.72518672682592	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	4.0
19	4.0
20	20.0
21	84.0
22	240.0
23	710.0
24	2004.0
25	4517.0
26	9223.0
27	16570.0
28	27419.0
29	42194.0
30	61764.0
31	88143.0
32	129342.0
33	211145.0
34	446932.0
35	1543272.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.76680229144062	17.68863953164744	11.86024519297208	26.68431298393986
2	16.156791981346785	20.24755610053662	36.8300758328766	26.765576085239996
3	18.189622706141044	24.462306804155315	28.330771645424203	29.017298844279438
4	12.576855356525638	16.176230953321873	35.68306389527428	35.5638497948782
5	14.612049062021134	36.77627267825701	33.168652729712605	15.443025530009248
6	34.4824356261965	35.20550150914505	16.901766617045634	13.410296247612811
7	30.531296628521996	30.12999378770197	20.83720876224316	18.501500821532872
8	28.312845667670565	32.80640442919089	19.113619940029114	19.76712996310943
9	27.733035241859348	14.106575185200812	17.956398703023403	40.20399086991643
10	15.814581789116977	26.80478331298032	31.603656188251506	25.7769787096512
11	37.27066284200997	20.801815460443173	22.608721904855535	19.318799792691323
12	25.381021683090903	23.03706484800161	28.676913430201505	22.905000038705982
13	29.837415209544915	19.44023301046031	24.913424228056087	25.808927551938687
14	23.941866779584785	19.09294185632898	24.976128113553926	31.9890632505323
15	25.38766763835983	26.834562755495238	21.96005903703672	25.81771056910821
16	26.043162905772306	25.39746497179668	23.35838015823896	25.20099196419206
17	24.50410998352291	25.533957246262656	24.821420761135585	25.140512009078854
18	25.196481315260726	24.714940011511164	25.541313039005193	24.547265634222914
19	26.053990869276607	24.62369149844112	24.780411714729727	24.541905917552548
20	26.28785374610712	24.099479710570133	24.520758549630315	25.09190799369243
21	26.871671052687603	23.963388885297842	24.10044639487658	25.064493667137977
22	26.20933796204728	24.04443751178112	24.407963669059598	25.338260857112004
23	24.941969720134775	23.883006620696595	24.961245410895412	26.21377824827322
24	25.35270907665118	24.525057149514588	24.729192970667764	25.393040803166468
25	25.376865166147354	24.03975150471251	24.911071974608582	25.67231135453156
26	25.287406152698015	24.59960548908979	25.030966319482616	25.082022038729573
27	25.642653371680286	24.56733877054923	24.29792949683173	25.492078360938752
28	25.2260025105584	24.23133986635902	24.985939556932504	25.55671806615008
29	25.301777493239857	24.483361111906753	24.735342318985758	25.47951907586763
30	25.20701575653794	24.492209983437192	24.946787283447758	25.353986976577115
31	25.698561246597045	24.236660206230738	24.331573261304698	25.733205285867516
32	25.39158889465623	24.549750668953475	24.21021299292862	25.848447443461676
33	25.007925377969887	24.28753851888524	24.769680194064346	25.93485590908053
34	25.58561710910024	24.173122344217994	24.978536984897314	25.262723561784455
35	26.017459132292043	24.115402286571147	24.792540716751066	25.074597864385744
36	25.31537773717683	24.538768526869525	24.545968313198447	25.599885422755197
37	25.87822721114767	24.354402941745693	24.40959938068512	25.35777046642152
38	25.362778296474875	24.39509854470627	24.72845819998173	25.51366495883713
39	25.774582957065395	24.277226864311686	24.31663097201573	25.631559206607196
40	26.091724184913033	24.378640370841733	24.63321473581862	24.896420708426618
41	25.28374364329857	24.541841397735006	24.883162105272937	25.291252853693486
42	26.01429431265954	24.787104540466682	24.443352060831145	24.755249086042628
43	25.410597502972653	24.1298026902497	24.848309614050734	25.61129019272691
44	25.29425494056842	24.46098579517634	24.43493645780878	25.80982280644646
45	25.34913119746582	24.64154057430921	24.50947453819464	25.49985369003033
46	25.361544996932533	24.386264715615823	24.58444049211071	25.66774979534093
47	25.5005649245161	24.410738636385627	24.782323107406103	25.306373331692168
48	25.88509336604296	24.839667727355593	24.14631701219373	25.128921894407714
49	25.393292088175457	24.718839314190873	24.333636788699074	25.554231808934592
50	25.40097989352618	25.022585237499158	24.220818982901797	25.35561588607286
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	24.0
1	35.5
2	47.0
3	148.0
4	249.0
5	265.5
6	282.0
7	280.5
8	279.0
9	368.0
10	457.0
11	686.5
12	916.0
13	1610.5
14	2305.0
15	3109.0
16	3913.0
17	4026.5
18	4140.0
19	4275.0
20	4410.0
21	4867.0
22	5324.0
23	6299.0
24	7274.0
25	9012.0
26	10750.0
27	13747.5
28	16745.0
29	20655.0
30	24565.0
31	29139.0
32	33713.0
33	40468.0
34	47223.0
35	55364.0
36	63505.0
37	75475.0
38	87445.0
39	96426.5
40	105408.0
41	117446.0
42	129484.0
43	135312.5
44	141141.0
45	154259.5
46	167378.0
47	179034.0
48	190690.0
49	199134.0
50	207578.0
51	205884.0
52	204190.0
53	202824.0
54	201458.0
55	200207.0
56	198956.0
57	193381.5
58	187807.0
59	171319.0
60	154831.0
61	136378.0
62	117925.0
63	101583.0
64	85241.0
65	72035.5
66	58830.0
67	49673.5
68	40517.0
69	35398.0
70	30279.0
71	23827.5
72	17376.0
73	14700.5
74	12025.0
75	9412.5
76	6800.0
77	5803.0
78	4806.0
79	4029.0
80	3252.0
81	2548.5
82	1845.0
83	1502.5
84	1160.0
85	838.5
86	517.0
87	371.5
88	226.0
89	161.5
90	97.0
91	90.0
92	83.0
93	68.0
94	53.0
95	43.5
96	34.0
97	25.5
98	17.0
99	17.0
100	17.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009057175159961713
2	4.257646442717044E-4
3	1.5482350700789252E-4
4	0.0
5	0.0
6	1.9352938375986567E-4
7	7.741175350394626E-5
8	0.0
9	0.0015482350700789253
10	8.515292885434089E-4
11	0.0
12	2.709411372638119E-4
13	4.644705210236775E-4
14	1.5482350700789252E-4
15	0.004799528717244668
16	0.0010063527955513013
17	0.0
18	3.0964701401578503E-4
19	7.741175350394626E-5
20	9.676469187993282E-4
21	0.0
22	1.5482350700789252E-4
23	8.515292885434089E-4
24	5.031763977756507E-4
25	0.0014321174398230058
26	0.005225293361516372
27	0.006076822650059781
28	0.0037931759216933664
29	0.002361058481870361
30	0.0034061171541736354
31	0.006579999047835432
32	0.0028642348796460116
33	0.004412469949724937
34	0.0028255290028940385
35	0.002631999619134173
36	0.006850940185099244
37	0.0033674112774216623
38	0.007392822459626868
39	0.003870587675197313
40	0.0022836467283664143
41	0.003599646537933501
42	0.0016256468235828713
43	0.0013547056863190594
44	0.0010837645490552475
45	0.001199882179311167
46	8.515292885434089E-4
47	0.002167529098110495
48	0.001780470330590764
49	4.257646442717044E-4
50	0.0015869409468308984
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2583587.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.7067068494585
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.45713296260887	39.77096986760837
2	13.632864374416492	14.370867722015932
3	4.519786084387135	7.1466912051616385
4	2.0501202321148657	4.322203443208881
5	1.1289542015794198	2.975172907455548
6	0.6849831671786892	2.1661924193580475
7	0.4591948013248194	1.6941852046155816
8	0.3383348225100975	1.4266011445602627
9	0.24108366917324356	1.1436053649575386
>10	1.344249454223167	12.642042927291833
>50	0.08091965306425279	2.9307935491916854
>100	0.05437718573205473	5.815530347746555
>500	0.005925475323665244	2.1384022552992805
>1k	0.0020739163632791696	1.456741641528857
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	3.870587675197313E-5	0.0
5	0.0	0.0	0.0	3.870587675197313E-5	0.0
6	0.0	0.0	0.0	3.870587675197313E-5	0.0
7	0.0	0.0	0.0	3.870587675197313E-5	0.0
8	0.0	0.0	0.0	3.870587675197313E-5	0.0
9	0.0	0.0	0.0	3.870587675197313E-5	0.0
10	0.0	0.0	0.0	1.5482350700789252E-4	0.0
11	0.0	0.0	0.0	1.5482350700789252E-4	0.0
12	0.0	0.0	0.0	1.5482350700789252E-4	0.0
13	0.0	0.0	0.0	1.5482350700789252E-4	3.870587675197313E-5
14	0.0	0.0	0.0	1.5482350700789252E-4	7.741175350394626E-5
15	0.0	0.0	0.0	2.3223526051183876E-4	7.741175350394626E-5
16	0.0	0.0	0.0	2.709411372638119E-4	1.1611763025591938E-4
17	0.0	0.0	0.0	3.0964701401578503E-4	1.1611763025591938E-4
18	0.0	0.0	0.0	4.257646442717044E-4	1.1611763025591938E-4
19	0.0	0.0	0.0	4.257646442717044E-4	1.1611763025591938E-4
20	0.0	0.0	0.0	5.418822745276238E-4	1.1611763025591938E-4
21	0.0	0.0	0.0	6.579999047835432E-4	1.5482350700789252E-4
22	0.0	0.0	0.0	8.902351652953819E-4	1.5482350700789252E-4
23	0.0	0.0	0.0	0.001470823316574979	1.5482350700789252E-4
24	0.0	0.0	0.0	0.002090117344606549	1.5482350700789252E-4
25	0.0	0.0	0.0	0.0024771761121262803	1.5482350700789252E-4
26	0.0	0.0	0.0	0.003057764263405877	1.5482350700789252E-4
27	0.0	0.0	0.0	0.0035222347844295547	1.5482350700789252E-4
28	0.0	0.0	0.0	0.004373764072972963	1.5482350700789252E-4
29	0.0	0.0	0.0	0.006541293171083459	1.5482350700789252E-4
30	0.0	0.0	0.0	0.010489292599784717	1.5482350700789252E-4
31	0.0	0.0	0.0	0.020320585294785892	1.5482350700789252E-4
32	0.0	0.0	0.0	0.03142917192260218	1.5482350700789252E-4
33	0.0	0.0	0.0	0.04358281722272174	1.5482350700789252E-4
34	0.0	0.0	0.0	0.056355756550872875	1.5482350700789252E-4
35	0.0	3.870587675197313E-5	0.0	0.07125751910038253	1.5482350700789252E-4
36	0.0	3.870587675197313E-5	0.0	0.09301022183499143	1.5482350700789252E-4
37	0.0	3.870587675197313E-5	0.0	0.12765198152800739	1.5482350700789252E-4
38	0.0	3.870587675197313E-5	0.0	0.17483444528866263	2.709411372638119E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	3440	0.0	24.55799	1
AACGCAG	4650	0.0	18.403833	6
TCAACGC	4845	0.0	17.66312	4
CAACGCA	4975	0.0	17.20157	5
TATCAAC	4990	0.0	17.061357	2
ATCAACG	5025	0.0	17.03041	3
ATACCGT	520	0.0	16.49958	6
TAATACC	350	0.0	16.34244	4
TAGGACG	500	0.0	16.279585	4
GGTATCA	1660	0.0	15.638457	1
CGACGGT	510	0.0	15.529015	7
TACGGGT	115	8.2250925E-5	15.303958	4
ACCGTCG	475	0.0	15.283822	8
ACGCAGA	5605	0.0	15.268121	7
GTACATG	8010	0.0	15.160954	1
TTAACGG	160	3.8277358E-7	15.125199	35
AGAGTAC	5775	0.0	14.8192425	11
ACGGTAT	535	0.0	14.803648	9
TACGACG	550	0.0	14.7996235	5
ACATGGG	7955	0.0	14.795349	3
>>END_MODULE