FastQCFastQC Report
Thu 2 Feb 2017
SRR4063885_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063885_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4747
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGG1182.4857804929429115No Hit
GTACAATGGTTGGCTGTGAACATCC210.4423846639983148No Hit
TCTAGAGACTGCCCCACCTGGGGAT190.4002527912365704No Hit
CCTATATACAGTCACCAAACCCAGA190.4002527912365704No Hit
CTATTGTGAATGCCAACAAGTGCTT180.3791868548556983No Hit
TCCTATGAGGCTGCAACCCCTTCAG160.33705498209395407No Hit
ATCTTACACTATTCCTGATTACCAT150.31598904571308195No Hit
CTTATAGGCCTATTAATCGCAGCTA140.2949231093322098No Hit
AATTAGGGCTGTGGTTTTTAAAAAT130.27385717295133766No Hit
CCATTGTACTGAGCACAGGGTCCCC130.27385717295133766No Hit
GCTATGTACAGCATACGAATCATTT130.27385717295133766No Hit
ATTTACAGCTATTTGTGCTCTCACC120.25279123657046554No Hit
ATTGTGAATGCCAACAAGTGCTTGC120.25279123657046554No Hit
GTACATAGCTGTTATAGAAGTGGCG120.25279123657046554No Hit
TCCTAATACTGAAATAATTAGGGCT110.23172530018959345No Hit
TATCAACGCAGAGTACATGGGGTGG110.23172530018959345No Hit
CTATGAGGCTGCAACCCCTTCAGCT110.23172530018959345No Hit
ATCCTATATACAGTCACCAAACCCA110.23172530018959345No Hit
GGTTAGTTCATATTGTTGTTCTTCC110.23172530018959345No Hit
GGTATCAACGCAGAGTACATGGGGT110.23172530018959345No Hit
GTCTTGTTCGTCTGCCAGGCTATGA110.23172530018959345No Hit
GTATCAACGCAGAGTACATGGGTGT110.23172530018959345No Hit
GTACATGGGCCTCCCCTTCAGTTGA100.21065936380872127No Hit
ATATTATATGAGATGACAAATCCTG100.21065936380872127No Hit
GTATATAGGATGGATCCCCAGGTGG100.21065936380872127No Hit
CTCTACATCAAGCCAACTAGGCCTG100.21065936380872127No Hit
GTGTAGGGCCTTCTATTGCTGATGG90.18959342742784915No Hit
ATATAATTCCCACCCCTTCTCAGCC90.18959342742784915No Hit
CTTCAACAGATTATATTCTCCAACA90.18959342742784915No Hit
CAACTATACTTTGCCTCGGAGCCCT90.18959342742784915No Hit
CTCTAACTCCTCCATTGGGGACCCT90.18959342742784915No Hit
ATATAATCTGTTGAAGTTCTCATGA90.18959342742784915No Hit
GTACTGAGCACAGGGTCCCCAATGG90.18959342742784915No Hit
CCTATTATAGCTGTCTCCTGAGAGG90.18959342742784915No Hit
GTACATGGGTCCCTCCATGTGTACT90.18959342742784915No Hit
CTCTATAACCGCATCGGAGACATCG90.18959342742784915No Hit
GTATCAACGCAGAGTACATGGGGTG80.16852749104697703No Hit
CTGTATATGCAGTGAATTCTTGCAG80.16852749104697703No Hit
GTTTTATGGTTAGGTTGTTTAGTTC80.16852749104697703No Hit
GAAATAATTAGGGCTGTGGTTTTTA80.16852749104697703No Hit
CCTTAGATGAAATGCCCTACAGTTG80.16852749104697703No Hit
GGCTCTACCAGTGCCTGACAAATAC80.16852749104697703No Hit
ATACCATTCCTAACAGGGTTCTACT80.16852749104697703No Hit
GGTGTAGGGCCTTCTATTGCTGATG80.16852749104697703No Hit
TGCTTATCCTCACCTCAGCCAACAA80.16852749104697703No Hit
TAACAAAACCGCGTTTTCCCCCCCT80.16852749104697703No Hit
CTATTATAGCTGTCTCCTGAGAGGC80.16852749104697703No Hit
ATACAGAGGTGGATGTTCACAGCCA80.16852749104697703No Hit
GTGTAGGAATGCTAGGTGTGGTTGG80.16852749104697703No Hit
GTTTACATCTGTAGCCCTTTTTGTC80.16852749104697703No Hit
GAGTGTAGTAGTGCTGAAACTGGTG80.16852749104697703No Hit
TATCAACGCAGAGTACATGGGTCCC80.16852749104697703No Hit
GTATTTGTCAGGCACTGGTAGAGCC80.16852749104697703No Hit
CCATGTGTACTCTTTGATTGGTGGT70.1474615546661049No Hit
CCTTGGGCCTGTCGATGGACCTTCT70.1474615546661049No Hit
ATTAATCGCAGCTACAGGAAAATCA70.1474615546661049No Hit
GGCTCAATCATTCATAGCCTGGCAG70.1474615546661049No Hit
GTACAGATGTGTAGGAATGCTAGGT70.1474615546661049No Hit
GCTCTGGGGGTACTGGTTAGTTCAT70.1474615546661049No Hit
GAATTATATCTTCCTACTAATTGGA70.1474615546661049No Hit
ATCCTGCAAAGATGCTTCCGAATGC70.1474615546661049No Hit
CTCTACAAGTTCCCTCTTTCAACTG70.1474615546661049No Hit
CTACTACACTCAAGTACAATAGTAG70.1474615546661049No Hit
ATAATTAGGTCTTTTGAGTAGAACC70.1474615546661049No Hit
TTCTCTAACTCCTCCATTGGGGACC70.1474615546661049No Hit
GTATATGCAGTGAATTCTTGCAGAG70.1474615546661049No Hit
ATGAATGATTGAGCCAGAGCATATA70.1474615546661049No Hit
ATCCTCACCTCAGCCAACAACATAT70.1474615546661049No Hit
CTATTATCAGGCCTAGTTGGCTTGA70.1474615546661049No Hit
GCATATAAAGAGTATAGCTTTGAAG70.1474615546661049No Hit
CATCTGTACCCACGCATTCTTCAAA70.1474615546661049No Hit
GATGTGTAGGAATGCTAGGTGTGGT70.1474615546661049No Hit
GTATTTGCGTCTGTTCGTCCGTACC70.1474615546661049No Hit
ACGCAGAGTACATGGGGTGGAGCAG70.1474615546661049No Hit
TTCATAGCCTGGCAGACGAACAAGA70.1474615546661049No Hit
ATCTAATTCCACTTATAGGCCTATT70.1474615546661049No Hit
GCCCTAACCACATTATTTACAGCTA60.12639561828523277No Hit
GTATCAACGCAGAGTACATGGGGTC60.12639561828523277No Hit
ATTATAGCTGTCTCCTGAGAGGCTC60.12639561828523277No Hit
CTCCATTTCCATCCCTGAAGTTCTT60.12639561828523277No Hit
TTACCATGCTTATCCTCACCTCAGC60.12639561828523277No Hit
CCCAAAACGACATCAAAAAATCATT60.12639561828523277No Hit
GCTATAATAGGTTTCTGTCAGCAAG60.12639561828523277No Hit
CTCCATGTGTACTCTTTGATTGGTG60.12639561828523277No Hit
TCTGTACCCACGCATTCTTCAAAGC60.12639561828523277No Hit
CTGCAAAGATGCTTCCGAATGCTAG60.12639561828523277No Hit
TTATATGAGATGACAAATCCTGCAA60.12639561828523277No Hit
GCATGATGATGTGAATGGTATGATT60.12639561828523277No Hit
CTCTTTATATGCTCTGGCTCAATCA60.12639561828523277No Hit
GTACTTGAGTGTAGTAGTGCTGAAA60.12639561828523277No Hit
ATCATTTACTTCGTAACAATAACAA60.12639561828523277No Hit
AATTAATACCTGCAACACCAACGCC60.12639561828523277No Hit
GATGTAAACAGGATAGAGAAAAAGT60.12639561828523277No Hit
CCAGAGACCTGGGAGGTGAGAGACT60.12639561828523277No Hit
GTATAGCTTTGAAGAATGCGTGGGT60.12639561828523277No Hit
CTATAATAGGTTTCTGTCAGCAAGC60.12639561828523277No Hit
CCTCTATAACCGCATCGGAGACATC60.12639561828523277No Hit
ACACTATTGTGAATGCCAACAAGTG60.12639561828523277No Hit
CTTGTAGAGTCTGCCTCCAGCAGGA60.12639561828523277No Hit
CATCTAAGGTCCCTCCCTTTGAGTG60.12639561828523277No Hit
GTTTAGTTCTAGTGCGATTAGGAAT60.12639561828523277No Hit
CTTTATATGCTCTGGCTCAATCATT60.12639561828523277No Hit
GTGGAATATTATATGAGATGACAAA60.12639561828523277No Hit
GTATTGTGAGGACTGGAATGCTGGT60.12639561828523277No Hit
CTTCAGCTCCTTGGGTCCTTTCTCT60.12639561828523277No Hit
CATATAAAGAGTATAGCTTTGAAGA60.12639561828523277No Hit
GATGTAGAGAAGGCAATGATTTTTT60.12639561828523277No Hit
GTATCAACGCAGAGTACATGGGTCC60.12639561828523277No Hit
CTGTAGGATACAACCCCATCCCTCA60.12639561828523277No Hit
ACTGAGCACAGGGTCCCCAATGGAG60.12639561828523277No Hit
TGGTATTCCTGTGAGGGCGAGGCTT50.10532968190436064No Hit
GCCTTCTATTGCTGATGGTAGTCAT50.10532968190436064No Hit
AATAATAACTTTATTTTAACAACTA50.10532968190436064No Hit
CTATTGTACTTGAGTGTAGTAGTGC50.10532968190436064No Hit
CACCTAGCATTCCTACACATCTGTA50.10532968190436064No Hit
TCTCAGGAGACAGCTATAATAGGTT50.10532968190436064No Hit
GTATATATCATGAAGAGAATTGTAT50.10532968190436064No Hit
GTTATAGAAGTGGCGATTAGTGTAA50.10532968190436064No Hit
GTTCAGTGATTGCACTGTCTTTCTT50.10532968190436064No Hit
GTCCCCAATGGAGGAGTTAGAGAAA50.10532968190436064No Hit
ATTCCTAACAGGGTTCTACTCAAAA50.10532968190436064No Hit
TATATACACTCAGACCCAAACATCA50.10532968190436064No Hit
ATTAATACCTGCAACACCAACGCCT50.10532968190436064No Hit
GGTTGGTGGAATATTATATGAGATG50.10532968190436064No Hit
GTATCAACGCAGAGTACATGGGCCT50.10532968190436064No Hit
TCATTTGAGGGATGGGGTTGTATCC50.10532968190436064No Hit
GTTATTATTAGTCGTGAGGGGGTGG50.10532968190436064No Hit
AATTAGGTCTTTTGAGTAGAACCCT50.10532968190436064No Hit
GTTCACAGCCAACCATTGTACTGAG50.10532968190436064No Hit
CCTCTGTATTTGTCAGGCACTGGTA50.10532968190436064No Hit
AGATTATATTCTCCAACAACAACGA50.10532968190436064No Hit
GTATAGTTGTTAAAATAAAGTTATT50.10532968190436064No Hit
GTTCTAGTGCGATTAGGAATCCTAA50.10532968190436064No Hit
GATTATATTCTCCAACAACAACGAC50.10532968190436064No Hit
TCCGAATGCTAGGCGTTTGATTGGG50.10532968190436064No Hit
CTTCAAAGCTATACTCTTTATATGC50.10532968190436064No Hit
GGCCTAGTTGGCTTGATGTAGAGAA50.10532968190436064No Hit
GAGTTACAGATGGCTCTAAGTCCCC50.10532968190436064No Hit
CATTATTTACAGCTATTTGTGCTCT50.10532968190436064No Hit
GTTACAGATGGCTCTAAGTCCCCAT50.10532968190436064No Hit
GGTCTTTTGAGTAGAACCCTGTTAG50.10532968190436064No Hit
GTCGTGAGGGGGTGGAATCGGACCA50.10532968190436064No Hit
GGGTACAGATGTGTAGGAATGCTAG50.10532968190436064No Hit
GCTGAGAAGGGGTGGGAATTATATC50.10532968190436064No Hit
GTGTAAGATATTTAATGAATCGATT50.10532968190436064No Hit
TCTCATGAGTTTATGTTTAGGGAAA50.10532968190436064No Hit
ACGCAGAGTACATGGGTCCCTCCAT50.10532968190436064No Hit
CCCAGACACTATTGTGAATGCCAAC50.10532968190436064No Hit
GGTAGAGCCTCTCAGGAGACAGCTA50.10532968190436064No Hit
GTCTCTAGATGATCATTCCTTCAGT50.10532968190436064No Hit
GTGTAGTAGTGCTGAAACTGGTGTA50.10532968190436064No Hit
GATTGGATGAGTGTTTTGGATTCTC50.10532968190436064No Hit
GCTCAGTACAATGGTTGGCTGTGAA50.10532968190436064No Hit
CATAAACCCAATCAAACGCCTAGCA50.10532968190436064No Hit
ATGTAGAGAAGGCAATGATTTTTTG50.10532968190436064No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers