##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063885_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4747
Sequences flagged as poor quality	0
Sequence length	25
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.406151253423214	32.0	32.0	32.0	32.0	32.0
2	31.12997682746998	32.0	32.0	32.0	32.0	32.0
3	31.02780703602275	32.0	32.0	32.0	32.0	32.0
4	31.045502422582683	32.0	32.0	32.0	32.0	32.0
5	31.077943964609226	32.0	32.0	32.0	32.0	32.0
6	34.63471666315568	36.0	36.0	36.0	32.0	36.0
7	34.58773962502633	36.0	36.0	36.0	32.0	36.0
8	34.59384874657678	36.0	36.0	36.0	32.0	36.0
9	34.68548556983358	36.0	36.0	36.0	32.0	36.0
10	34.47019170002107	36.0	36.0	36.0	32.0	36.0
11	34.74320623551717	36.0	36.0	36.0	36.0	36.0
12	34.63092479460712	36.0	36.0	36.0	32.0	36.0
13	34.57994522856541	36.0	36.0	36.0	32.0	36.0
14	34.526437750157996	36.0	36.0	36.0	32.0	36.0
15	34.54371181799031	36.0	36.0	36.0	32.0	36.0
16	34.49926269222667	36.0	36.0	36.0	32.0	36.0
17	34.475458184116285	36.0	36.0	36.0	32.0	36.0
18	34.490204339582895	36.0	36.0	36.0	32.0	36.0
19	34.43648620181167	36.0	36.0	36.0	32.0	36.0
20	34.35116915946914	36.0	36.0	36.0	32.0	36.0
21	34.25890035812092	36.0	36.0	36.0	32.0	36.0
22	34.44491257636402	36.0	36.0	36.0	32.0	36.0
23	34.39667158205182	36.0	36.0	36.0	32.0	36.0
24	34.40425531914894	36.0	36.0	36.0	32.0	36.0
25	33.969243732883925	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	0.0
8	0.0
9	1.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	1.0
17	1.0
18	2.0
19	4.0
20	7.0
21	5.0
22	11.0
23	16.0
24	20.0
25	31.0
26	45.0
27	46.0
28	69.0
29	111.0
30	140.0
31	195.0
32	258.0
33	428.0
34	900.0
35	2455.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.49726085124315	20.79646017699115	13.147914032869783	25.55836493889591
2	16.72635348641247	20.728881398778178	40.29913629660838	22.24562881820097
3	17.90604592374131	28.733937223509585	30.81946492521593	22.54055192753318
4	15.14640825784706	18.30629871497788	39.182641668422164	27.364651358752894
5	15.29709228824273	37.69490096923725	35.35608933839022	11.651917404129794
6	29.35721812434141	37.32349841938883	20.505795574288722	12.813487881981034
7	27.529510961214164	31.281618887015178	26.15935919055649	15.029510961214164
8	27.171163575042158	34.970489038785836	21.85919055649241	15.999156829679595
9	28.604553119730188	15.61973018549747	20.59443507588533	35.18128161888702
10	18.08600337268128	27.887858347386175	33.031197301854974	20.994940978077572
11	33.93760539629005	22.470489038785836	24.578414839797638	19.013490725126474
12	24.578414839797638	25.35834738617201	28.54131534569983	21.521922428330523
13	28.520236087689714	22.74451939291737	26.475548060708263	22.259696458684655
14	22.887249736564804	21.74920969441517	27.650158061116965	27.713382507903056
15	24.936762225969648	28.81534569983137	23.77740303541315	22.470489038785836
16	23.14990512333966	29.03225806451613	25.173940543959517	22.643896268184694
17	24.009274873524454	26.22259696458685	27.360876897133217	22.40725126475548
18	24.641652613827993	26.83389544688027	27.25548060708263	21.268971332209105
19	24.768128161888704	27.276559865092747	27.930016863406408	20.02529510961214
20	26.797385620915033	25.49019607843137	26.41787897954881	21.294539321104786
21	27.32447817836812	26.12270714737508	25.363693864642634	21.189120809614167
22	28.69491882774615	26.143790849673206	23.972169512966477	21.189120809614167
23	25.405861269238876	25.63778199451824	26.523297491039425	22.433059245203456
24	25.63778199451824	26.333544170356316	24.794433902593298	23.23423993253215
25	24.39384355892895	26.54438119333755	27.092557453088762	21.96921779464474
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2.0
1	2.0
2	2.0
3	2.0
4	2.0
5	2.0
6	4.0
7	6.0
8	6.0
9	6.0
10	8.0
11	10.0
12	10.0
13	10.0
14	15.0
15	20.0
16	20.0
17	20.0
18	30.5
19	41.0
20	41.0
21	41.0
22	91.5
23	142.0
24	142.0
25	142.0
26	178.0
27	214.0
28	214.0
29	214.0
30	293.0
31	372.0
32	372.0
33	372.0
34	398.0
35	424.0
36	424.0
37	424.0
38	484.0
39	544.0
40	544.0
41	544.0
42	603.0
43	662.0
44	662.0
45	662.0
46	657.5
47	653.0
48	653.0
49	653.0
50	621.0
51	589.0
52	589.0
53	589.0
54	461.5
55	334.0
56	334.0
57	334.0
58	309.0
59	284.0
60	284.0
61	284.0
62	209.5
63	135.0
64	135.0
65	135.0
66	106.5
67	78.0
68	78.0
69	78.0
70	62.0
71	46.0
72	46.0
73	46.0
74	43.0
75	40.0
76	40.0
77	40.0
78	27.0
79	14.0
80	14.0
81	14.0
82	9.0
83	4.0
84	4.0
85	4.0
86	4.0
87	4.0
88	4.0
89	4.0
90	2.5
91	1.0
92	1.0
93	1.0
94	1.5
95	2.0
96	2.0
97	2.0
98	63.0
99	124.0
100	124.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.02106593638087213
2	0.0
3	0.0
4	0.0
5	0.02106593638087213
6	0.04213187276174426
7	0.06319780914261638
8	0.06319780914261638
9	0.06319780914261638
10	0.06319780914261638
11	0.06319780914261638
12	0.06319780914261638
13	0.06319780914261638
14	0.04213187276174426
15	0.06319780914261638
16	0.08426374552348852
17	0.06319780914261638
18	0.06319780914261638
19	0.06319780914261638
20	0.08426374552348852
21	0.08426374552348852
22	0.08426374552348852
23	0.08426374552348852
24	0.08426374552348852
25	0.08426374552348852
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	4747.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.76406151253423
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.03101868170603	45.439224773541184
2	11.350017624250968	13.566463029281653
3	4.406062742333451	7.899726142827049
4	2.7493831512160734	6.572572150832104
5	1.5861825872400421	4.73983568569623
6	1.1984490659146985	4.297451021697914
7	0.8107155445893549	3.3916157573204133
8	0.5992245329573492	2.8649673477986095
9	0.3524850193866761	1.8959342742784917
>10	0.8812125484666901	6.846429323783443
>50	0.0	0.0
>100	0.035248501938667604	2.4857804929429115
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGGGGGGGGGGGGGGGGGGGGGGGG	118	2.4857804929429115	No Hit
GTACAATGGTTGGCTGTGAACATCC	21	0.4423846639983148	No Hit
TCTAGAGACTGCCCCACCTGGGGAT	19	0.4002527912365704	No Hit
CCTATATACAGTCACCAAACCCAGA	19	0.4002527912365704	No Hit
CTATTGTGAATGCCAACAAGTGCTT	18	0.3791868548556983	No Hit
TCCTATGAGGCTGCAACCCCTTCAG	16	0.33705498209395407	No Hit
ATCTTACACTATTCCTGATTACCAT	15	0.31598904571308195	No Hit
CTTATAGGCCTATTAATCGCAGCTA	14	0.2949231093322098	No Hit
AATTAGGGCTGTGGTTTTTAAAAAT	13	0.27385717295133766	No Hit
CCATTGTACTGAGCACAGGGTCCCC	13	0.27385717295133766	No Hit
GCTATGTACAGCATACGAATCATTT	13	0.27385717295133766	No Hit
ATTTACAGCTATTTGTGCTCTCACC	12	0.25279123657046554	No Hit
ATTGTGAATGCCAACAAGTGCTTGC	12	0.25279123657046554	No Hit
GTACATAGCTGTTATAGAAGTGGCG	12	0.25279123657046554	No Hit
TCCTAATACTGAAATAATTAGGGCT	11	0.23172530018959345	No Hit
TATCAACGCAGAGTACATGGGGTGG	11	0.23172530018959345	No Hit
CTATGAGGCTGCAACCCCTTCAGCT	11	0.23172530018959345	No Hit
ATCCTATATACAGTCACCAAACCCA	11	0.23172530018959345	No Hit
GGTTAGTTCATATTGTTGTTCTTCC	11	0.23172530018959345	No Hit
GGTATCAACGCAGAGTACATGGGGT	11	0.23172530018959345	No Hit
GTCTTGTTCGTCTGCCAGGCTATGA	11	0.23172530018959345	No Hit
GTATCAACGCAGAGTACATGGGTGT	11	0.23172530018959345	No Hit
GTACATGGGCCTCCCCTTCAGTTGA	10	0.21065936380872127	No Hit
ATATTATATGAGATGACAAATCCTG	10	0.21065936380872127	No Hit
GTATATAGGATGGATCCCCAGGTGG	10	0.21065936380872127	No Hit
CTCTACATCAAGCCAACTAGGCCTG	10	0.21065936380872127	No Hit
GTGTAGGGCCTTCTATTGCTGATGG	9	0.18959342742784915	No Hit
ATATAATTCCCACCCCTTCTCAGCC	9	0.18959342742784915	No Hit
CTTCAACAGATTATATTCTCCAACA	9	0.18959342742784915	No Hit
CAACTATACTTTGCCTCGGAGCCCT	9	0.18959342742784915	No Hit
CTCTAACTCCTCCATTGGGGACCCT	9	0.18959342742784915	No Hit
ATATAATCTGTTGAAGTTCTCATGA	9	0.18959342742784915	No Hit
GTACTGAGCACAGGGTCCCCAATGG	9	0.18959342742784915	No Hit
CCTATTATAGCTGTCTCCTGAGAGG	9	0.18959342742784915	No Hit
GTACATGGGTCCCTCCATGTGTACT	9	0.18959342742784915	No Hit
CTCTATAACCGCATCGGAGACATCG	9	0.18959342742784915	No Hit
GTATCAACGCAGAGTACATGGGGTG	8	0.16852749104697703	No Hit
CTGTATATGCAGTGAATTCTTGCAG	8	0.16852749104697703	No Hit
GTTTTATGGTTAGGTTGTTTAGTTC	8	0.16852749104697703	No Hit
GAAATAATTAGGGCTGTGGTTTTTA	8	0.16852749104697703	No Hit
CCTTAGATGAAATGCCCTACAGTTG	8	0.16852749104697703	No Hit
GGCTCTACCAGTGCCTGACAAATAC	8	0.16852749104697703	No Hit
ATACCATTCCTAACAGGGTTCTACT	8	0.16852749104697703	No Hit
GGTGTAGGGCCTTCTATTGCTGATG	8	0.16852749104697703	No Hit
TGCTTATCCTCACCTCAGCCAACAA	8	0.16852749104697703	No Hit
TAACAAAACCGCGTTTTCCCCCCCT	8	0.16852749104697703	No Hit
CTATTATAGCTGTCTCCTGAGAGGC	8	0.16852749104697703	No Hit
ATACAGAGGTGGATGTTCACAGCCA	8	0.16852749104697703	No Hit
GTGTAGGAATGCTAGGTGTGGTTGG	8	0.16852749104697703	No Hit
GTTTACATCTGTAGCCCTTTTTGTC	8	0.16852749104697703	No Hit
GAGTGTAGTAGTGCTGAAACTGGTG	8	0.16852749104697703	No Hit
TATCAACGCAGAGTACATGGGTCCC	8	0.16852749104697703	No Hit
GTATTTGTCAGGCACTGGTAGAGCC	8	0.16852749104697703	No Hit
CCATGTGTACTCTTTGATTGGTGGT	7	0.1474615546661049	No Hit
CCTTGGGCCTGTCGATGGACCTTCT	7	0.1474615546661049	No Hit
ATTAATCGCAGCTACAGGAAAATCA	7	0.1474615546661049	No Hit
GGCTCAATCATTCATAGCCTGGCAG	7	0.1474615546661049	No Hit
GTACAGATGTGTAGGAATGCTAGGT	7	0.1474615546661049	No Hit
GCTCTGGGGGTACTGGTTAGTTCAT	7	0.1474615546661049	No Hit
GAATTATATCTTCCTACTAATTGGA	7	0.1474615546661049	No Hit
ATCCTGCAAAGATGCTTCCGAATGC	7	0.1474615546661049	No Hit
CTCTACAAGTTCCCTCTTTCAACTG	7	0.1474615546661049	No Hit
CTACTACACTCAAGTACAATAGTAG	7	0.1474615546661049	No Hit
ATAATTAGGTCTTTTGAGTAGAACC	7	0.1474615546661049	No Hit
TTCTCTAACTCCTCCATTGGGGACC	7	0.1474615546661049	No Hit
GTATATGCAGTGAATTCTTGCAGAG	7	0.1474615546661049	No Hit
ATGAATGATTGAGCCAGAGCATATA	7	0.1474615546661049	No Hit
ATCCTCACCTCAGCCAACAACATAT	7	0.1474615546661049	No Hit
CTATTATCAGGCCTAGTTGGCTTGA	7	0.1474615546661049	No Hit
GCATATAAAGAGTATAGCTTTGAAG	7	0.1474615546661049	No Hit
CATCTGTACCCACGCATTCTTCAAA	7	0.1474615546661049	No Hit
GATGTGTAGGAATGCTAGGTGTGGT	7	0.1474615546661049	No Hit
GTATTTGCGTCTGTTCGTCCGTACC	7	0.1474615546661049	No Hit
ACGCAGAGTACATGGGGTGGAGCAG	7	0.1474615546661049	No Hit
TTCATAGCCTGGCAGACGAACAAGA	7	0.1474615546661049	No Hit
ATCTAATTCCACTTATAGGCCTATT	7	0.1474615546661049	No Hit
GCCCTAACCACATTATTTACAGCTA	6	0.12639561828523277	No Hit
GTATCAACGCAGAGTACATGGGGTC	6	0.12639561828523277	No Hit
ATTATAGCTGTCTCCTGAGAGGCTC	6	0.12639561828523277	No Hit
CTCCATTTCCATCCCTGAAGTTCTT	6	0.12639561828523277	No Hit
TTACCATGCTTATCCTCACCTCAGC	6	0.12639561828523277	No Hit
CCCAAAACGACATCAAAAAATCATT	6	0.12639561828523277	No Hit
GCTATAATAGGTTTCTGTCAGCAAG	6	0.12639561828523277	No Hit
CTCCATGTGTACTCTTTGATTGGTG	6	0.12639561828523277	No Hit
TCTGTACCCACGCATTCTTCAAAGC	6	0.12639561828523277	No Hit
CTGCAAAGATGCTTCCGAATGCTAG	6	0.12639561828523277	No Hit
TTATATGAGATGACAAATCCTGCAA	6	0.12639561828523277	No Hit
GCATGATGATGTGAATGGTATGATT	6	0.12639561828523277	No Hit
CTCTTTATATGCTCTGGCTCAATCA	6	0.12639561828523277	No Hit
GTACTTGAGTGTAGTAGTGCTGAAA	6	0.12639561828523277	No Hit
ATCATTTACTTCGTAACAATAACAA	6	0.12639561828523277	No Hit
AATTAATACCTGCAACACCAACGCC	6	0.12639561828523277	No Hit
GATGTAAACAGGATAGAGAAAAAGT	6	0.12639561828523277	No Hit
CCAGAGACCTGGGAGGTGAGAGACT	6	0.12639561828523277	No Hit
GTATAGCTTTGAAGAATGCGTGGGT	6	0.12639561828523277	No Hit
CTATAATAGGTTTCTGTCAGCAAGC	6	0.12639561828523277	No Hit
CCTCTATAACCGCATCGGAGACATC	6	0.12639561828523277	No Hit
ACACTATTGTGAATGCCAACAAGTG	6	0.12639561828523277	No Hit
CTTGTAGAGTCTGCCTCCAGCAGGA	6	0.12639561828523277	No Hit
CATCTAAGGTCCCTCCCTTTGAGTG	6	0.12639561828523277	No Hit
GTTTAGTTCTAGTGCGATTAGGAAT	6	0.12639561828523277	No Hit
CTTTATATGCTCTGGCTCAATCATT	6	0.12639561828523277	No Hit
GTGGAATATTATATGAGATGACAAA	6	0.12639561828523277	No Hit
GTATTGTGAGGACTGGAATGCTGGT	6	0.12639561828523277	No Hit
CTTCAGCTCCTTGGGTCCTTTCTCT	6	0.12639561828523277	No Hit
CATATAAAGAGTATAGCTTTGAAGA	6	0.12639561828523277	No Hit
GATGTAGAGAAGGCAATGATTTTTT	6	0.12639561828523277	No Hit
GTATCAACGCAGAGTACATGGGTCC	6	0.12639561828523277	No Hit
CTGTAGGATACAACCCCATCCCTCA	6	0.12639561828523277	No Hit
ACTGAGCACAGGGTCCCCAATGGAG	6	0.12639561828523277	No Hit
TGGTATTCCTGTGAGGGCGAGGCTT	5	0.10532968190436064	No Hit
GCCTTCTATTGCTGATGGTAGTCAT	5	0.10532968190436064	No Hit
AATAATAACTTTATTTTAACAACTA	5	0.10532968190436064	No Hit
CTATTGTACTTGAGTGTAGTAGTGC	5	0.10532968190436064	No Hit
CACCTAGCATTCCTACACATCTGTA	5	0.10532968190436064	No Hit
TCTCAGGAGACAGCTATAATAGGTT	5	0.10532968190436064	No Hit
GTATATATCATGAAGAGAATTGTAT	5	0.10532968190436064	No Hit
GTTATAGAAGTGGCGATTAGTGTAA	5	0.10532968190436064	No Hit
GTTCAGTGATTGCACTGTCTTTCTT	5	0.10532968190436064	No Hit
GTCCCCAATGGAGGAGTTAGAGAAA	5	0.10532968190436064	No Hit
ATTCCTAACAGGGTTCTACTCAAAA	5	0.10532968190436064	No Hit
TATATACACTCAGACCCAAACATCA	5	0.10532968190436064	No Hit
ATTAATACCTGCAACACCAACGCCT	5	0.10532968190436064	No Hit
GGTTGGTGGAATATTATATGAGATG	5	0.10532968190436064	No Hit
GTATCAACGCAGAGTACATGGGCCT	5	0.10532968190436064	No Hit
TCATTTGAGGGATGGGGTTGTATCC	5	0.10532968190436064	No Hit
GTTATTATTAGTCGTGAGGGGGTGG	5	0.10532968190436064	No Hit
AATTAGGTCTTTTGAGTAGAACCCT	5	0.10532968190436064	No Hit
GTTCACAGCCAACCATTGTACTGAG	5	0.10532968190436064	No Hit
CCTCTGTATTTGTCAGGCACTGGTA	5	0.10532968190436064	No Hit
AGATTATATTCTCCAACAACAACGA	5	0.10532968190436064	No Hit
GTATAGTTGTTAAAATAAAGTTATT	5	0.10532968190436064	No Hit
GTTCTAGTGCGATTAGGAATCCTAA	5	0.10532968190436064	No Hit
GATTATATTCTCCAACAACAACGAC	5	0.10532968190436064	No Hit
TCCGAATGCTAGGCGTTTGATTGGG	5	0.10532968190436064	No Hit
CTTCAAAGCTATACTCTTTATATGC	5	0.10532968190436064	No Hit
GGCCTAGTTGGCTTGATGTAGAGAA	5	0.10532968190436064	No Hit
GAGTTACAGATGGCTCTAAGTCCCC	5	0.10532968190436064	No Hit
CATTATTTACAGCTATTTGTGCTCT	5	0.10532968190436064	No Hit
GTTACAGATGGCTCTAAGTCCCCAT	5	0.10532968190436064	No Hit
GGTCTTTTGAGTAGAACCCTGTTAG	5	0.10532968190436064	No Hit
GTCGTGAGGGGGTGGAATCGGACCA	5	0.10532968190436064	No Hit
GGGTACAGATGTGTAGGAATGCTAG	5	0.10532968190436064	No Hit
GCTGAGAAGGGGTGGGAATTATATC	5	0.10532968190436064	No Hit
GTGTAAGATATTTAATGAATCGATT	5	0.10532968190436064	No Hit
TCTCATGAGTTTATGTTTAGGGAAA	5	0.10532968190436064	No Hit
ACGCAGAGTACATGGGTCCCTCCAT	5	0.10532968190436064	No Hit
CCCAGACACTATTGTGAATGCCAAC	5	0.10532968190436064	No Hit
GGTAGAGCCTCTCAGGAGACAGCTA	5	0.10532968190436064	No Hit
GTCTCTAGATGATCATTCCTTCAGT	5	0.10532968190436064	No Hit
GTGTAGTAGTGCTGAAACTGGTGTA	5	0.10532968190436064	No Hit
GATTGGATGAGTGTTTTGGATTCTC	5	0.10532968190436064	No Hit
GCTCAGTACAATGGTTGGCTGTGAA	5	0.10532968190436064	No Hit
CATAAACCCAATCAAACGCCTAGCA	5	0.10532968190436064	No Hit
ATGTAGAGAAGGCAATGATTTTTTG	5	0.10532968190436064	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.02106593638087213	0.0
6	0.0	0.0	0.0	0.02106593638087213	0.0
7	0.0	0.0	0.0	0.02106593638087213	0.0
8	0.0	0.0	0.0	0.02106593638087213	0.0
9	0.0	0.0	0.0	0.02106593638087213	0.0
10	0.0	0.0	0.0	0.02106593638087213	0.0
11	0.0	0.0	0.0	0.02106593638087213	0.0
12	0.0	0.0	0.0	0.02106593638087213	0.0
13	0.0	0.0	0.0	0.02106593638087213	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE