FastQCFastQC Report
Thu 2 Feb 2017
SRR4063885_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063885_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4747
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTATATACAGTCACCAAACCCAGACACTATTGTGAATGCCAACAAGTGC180.3791868548556983No Hit
GTACAATGGTTGGCTGTGAACATCCACCTCTGTATTTGTCAGGCACTGGT160.33705498209395407No Hit
CAACTATACTTTGCCTCGGAGCCCTAACCACATTATTTACAGCTATTTGT160.33705498209395407No Hit
CCATTGTACTGAGCACAGGGTCCCCAATGGAGGAGTTAGAGAAAGGACCC150.31598904571308195No Hit
TCCTATGAGGCTGCAACCCCTTCAGCTCCTTGGGTCCTTTCTCTAACTCC150.31598904571308195No Hit
CTATTGTGAATGCCAACAAGTGCTTGCTGACAGAAACCTATTATAGCTGT140.2949231093322098No Hit
GTATTCCTGTGAGGGCGAGGCTTCCGATTACTAGGCATGATGATGTGAAT140.2949231093322098No Hit
CTTATAGGCCTATTAATCGCAGCTACAGGAAAATCAGCACAATTTGGCCT130.27385717295133766No Hit
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATGTCGTATGCCGT130.27385717295133766No Hit
AATTAGGGCTGTGGTTTTTAAAAATCATGGTATTGTGAGGACTGGAATGC130.27385717295133766No Hit
CTCTACATCAAGCCAACTAGGCCTGATAATAGTGACGCTAGGAATAAACC130.27385717295133766No Hit
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGT130.27385717295133766No Hit
TCTAGAGACTGCCCCACCTGGGGATCCATCCTATATACAGTCACCAAACC130.27385717295133766No Hit
CTATTATAGCTGTCTCCTGAGAGGCTCTACCAGTGCCTGACAAATACAGA130.27385717295133766No Hit
ATCCTATATACAGTCACCAAACCCAGACACTATTGTGAATGCCAACAAGT120.25279123657046554No Hit
TCACAATAGTGTCTGGGTTTGGTGACTGTATATAGGATGGATCCCCAGGT120.25279123657046554No Hit
GAATTATATCTTCCTACTAATTGGATGATGGTACGGACGAACAGACGCAA120.25279123657046554No Hit
CTTTATATGCTCTGGCTCAATCATTCATAGCCTGGCAGACGAACAAGACA110.23172530018959345No Hit
ATCTAATTCCACTTATAGGCCTATTAATCGCAGCTACAGGAAAATCAGCA110.23172530018959345No Hit
ATATTCCACCAACCAGCATTCCAGTCCTCACAATACCATGATTTTTAAAA110.23172530018959345No Hit
ATATTATATGAGATGACAAATCCTGCAAAGATGCTTCCGAATGCTAGGCG110.23172530018959345No Hit
GTTATAGAGGATTGCTTGTAGGGCTGCAGTATTTGCGTCTGTTCGTCCGT110.23172530018959345No Hit
GTTTAGTTCTAGTGCGATTAGGAATCCTAATACTGAAATAATTAGGGCTG100.21065936380872127No Hit
CTTCAAAGCTATACTCTTTATATGCTCTGGCTCAATCATTCATAGCCTGG100.21065936380872127No Hit
TCCTAATACTGAAATAATTAGGGCTGTGGTTTTTAAAAATCATGGTATTG100.21065936380872127No Hit
GGTTAGTTCATATTGTTGTTCTTCCTATGAGGCTGCAACCCCTTCAGCTC100.21065936380872127No Hit
CCTCTGTATTTGTCAGGCACTGGTAGAGCCTCTCAGGAGACAGCTATAAT100.21065936380872127No Hit
GTATCAACGCAGAGTACATGGGGTGGAGCAGAGACTGAAGGAATGATCAT90.18959342742784915No Hit
ATCTTACACTATTCCTGATTACCATGCTTATCCTCACCTCAGCCAACAAC90.18959342742784915No Hit
GCTATGTACAGCATACGAATCATTTACTTCGTAACAATAACAAAACCGCG90.18959342742784915No Hit
ATATAATCTGTTGAAGTTCTCATGAGTTTATGTTTAGGGAAAATCAAACT90.18959342742784915No Hit
GTATTTGTCAGGCACTGGTAGAGCCTCTCAGGAGACAGCTATAATAGGTT90.18959342742784915No Hit
GTATCAACGCAGAGTACATGGGCCTCCCCTTCAGTTGATACCACCTTGGG90.18959342742784915No Hit
GTACATGGGTCCCTCCATGTGTACTCTTTGATTGGTGGTTTAGTCCCTGA90.18959342742784915No Hit
CTATTGTACTTGAGTGTAGTAGTGCTGAAACTGGTGTAGGGCCTTCTATT80.16852749104697703No Hit
GCATACGAATCATTTACTTCGTAACAATAACAAAACCGCGTTTTCCCCCC80.16852749104697703No Hit
CTTGTAGAGTCTGCCTCCAGCAGGAAGACAGGACATCATTTGAGGGATGG80.16852749104697703No Hit
CTTCAGCTCCTTGGGTCCTTTCTCTAACTCCTCCATTGGGGACCCTGTGC80.16852749104697703No Hit
TCTCAGGAGACAGCTATAATAGGTTTCTGTCAGCAAGCACTTGTTGGCAT80.16852749104697703No Hit
CTCTAACTCCTCCATTGGGGACCCTGTGCTCAGTACAATGGTTGGCTGTG80.16852749104697703No Hit
TATCAACGCAGAGTACATGGGGTGGAGCAGAGACTGAAGGAATGATCATC80.16852749104697703No Hit
GTATATAGGATGGATCCCCAGGTGGGGCAGTCTCTAGATGATCATTCCTT80.16852749104697703No Hit
TAGTAGGGCTCAGGCGTTGGTGTTGCAGGTATTAATTGCTTCAATAATTA80.16852749104697703No Hit
GTACATAGCTGTTATAGAAGTGGCGATTAGTGTAATTAGTAGGGCTCAGG80.16852749104697703No Hit
TTCATAGCCTGGCAGACGAACAAGACATCCGAAAAATAGGAAACATCACA80.16852749104697703No Hit
GTGTTTATGTCTGTGCATACTGTATATGCAGTGAATTCTTGCAGAGATTG70.1474615546661049No Hit
ACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCT70.1474615546661049RNA PCR Primer, Index 12 (95% over 24bp)
ATACAGAGGTGGATGTTCACAGCCAACCATTGTACTGAGCACAGGGTCCC70.1474615546661049No Hit
ATTTACAGCTATTTGTGCTCTCACCCAAAACGACATCAAAAAATCATTGC70.1474615546661049No Hit
GTCTTTCTTGCTGAGAACCCAGATTTGATTCCCAGCACTCACAGATGGGG70.1474615546661049No Hit
ATTATAGCTGTCTCCTGAGAGGCTCTACCAGTGCCTGACAAATACAGAGG70.1474615546661049No Hit
CCATGTGTACTCTTTGATTGGTGGTTTAGTCCCTGAAGCTCTGGGGGTAC70.1474615546661049No Hit
ACGCAGAGTACATGGGGTGGAGCAGAGACTGAAGGAATGATCATCTAGAG70.1474615546661049No Hit
TTGTTGTTCTTCCTATGAGGCTGCAACCCCTTCAGCTCCTTGGGTCCTTT70.1474615546661049No Hit
CTACTATTGTACTTGAGTGTAGTAGTGCTGAAACTGGTGTAGGGCCTTCT70.1474615546661049No Hit
ATGTAAAACCACGACCTTTTAAAACAAAATAAGGGGCTGGAGACATGGTT70.1474615546661049No Hit
CTGTATATGCAGTGAATTCTTGCAGAGATTGGATGAGTGTTTTGGATTCT70.1474615546661049No Hit
GTACTTGAGTGTAGTAGTGCTGAAACTGGTGTAGGGCCTTCTATTGCTGA70.1474615546661049No Hit
GCTATAATAGGTTTCTGTCAGCAAGCACTTGTTGGCATTCACAATAGTGT70.1474615546661049No Hit
GAAATAATTAGGGCTGTGGTTTTTAAAAATCATGGTATTGTGAGGACTGG70.1474615546661049No Hit
GTAATTAGTAGGGCTCAGGCGTTGGTGTTGCAGGTATTAATTGCTTCAAT70.1474615546661049No Hit
GCGATTAATAGGCCTATAAGTGGAATTAGATTGTCGTTGTTGTTGGAGAA70.1474615546661049No Hit
ATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTT60.12639561828523277TruSeq Adapter, Index 12 (95% over 22bp)
ATTGTGAATGCCAACAAGTGCTTGCTGACAGAAACCTATTATAGCTGTCT60.12639561828523277No Hit
ATTATTAGTCGTGAGGGGGTGGAATCGGACCAGTAGGAAAATTCCTGCAA60.12639561828523277No Hit
TCATCATGCCTAGTAATCGGAAGCCTCGCCCTCACAGGAATACCATTCCT60.12639561828523277No Hit
GTATTGTGAGGACTGGAATGCTGGTTGGTGGAATATTATATGAGATGACA60.12639561828523277No Hit
TCCGAATGCTAGGCGTTTGATTGGGTTTATGAGGTCTGGGTCATTTTCGT60.12639561828523277No Hit
ATTGTGAGGACTGGAATGCTGGTTGGTGGAATATTATATGAGATGACAAA60.12639561828523277No Hit
ATAATGAACCAGAGACCTGGGAGGTGAGAGACTCTCAGCACTCAAAGGGA60.12639561828523277No Hit
GTTAGGAATGGTATTCCTGTGAGGGCGAGGCTTCCGATTACTAGGCATGA60.12639561828523277No Hit
TCTCTACATCAAGCCAACTAGGCCTGATAATAGTGACGCTAGGAATAAAC60.12639561828523277No Hit
GTTATTATTAGTCGTGAGGGGGTGGAATCGGACCAGTAGGAAAATTCCTG60.12639561828523277No Hit
CTCTGGGACTAGAGTTACAGATGGCTCTAAGTCCCCATCTGTGAGTGCTG60.12639561828523277No Hit
GAATTAGATTGTCGTTGTTGTTGGAGAATATAATCTGTTGAAGTTCTCAT60.12639561828523277No Hit
ATATAATTCCCACCCCTTCTCAGCCAATGAAAAGTTGAAATATGTTGTTG60.12639561828523277No Hit
GCCTATAAGTGGAATTAGATTGTCGTTGTTGTTGGAGAATATAATCTGTT60.12639561828523277No Hit
ATCCTCACCTCAGCCAACAACATATTTCAACTTTTCATTGGCTGAGAAGG60.12639561828523277No Hit
ATATTGTTGTTCTTCCTATGAGGCTGCAACCCCTTCAGCTCCTTGGGTCC60.12639561828523277No Hit
TATATACACTCAGACCCAAACATCAATCGATTCATTAAATATCTTACACT60.12639561828523277No Hit
CCATAAATTCAATAGAACTTAAAATAAGCTTCAAAACTGACTTTTTCTCT60.12639561828523277No Hit
GTATAGCTTTGAAGAATGCGTGGGTACAGATGTGTAGGAATGCTAGGTGT60.12639561828523277No Hit
GGCTCTACCAGTGCCTGACAAATACAGAGGTGGATGTTCACAGCCAACCA60.12639561828523277No Hit
CCATTAACGAAAATGACCCAGACCTCATAAACCCAATCAAACGCCTAGCA60.12639561828523277No Hit
GCCCTAACCACATTATTTACAGCTATTTGTGCTCTCACCCAAAACGACAT60.12639561828523277No Hit
GGTATTGGGTGGGGGAAAAGAACTGAAGACCTGAGGGCCAGCAAAGAGAA60.12639561828523277No Hit
GTGATTGCACTGTCTTTCTTGCTGAGAACCCAGATTTGATTCCCAGCACT60.12639561828523277No Hit
GTATAGTTGTTAAAATAAAGTTATTATTAGTCGTGAGGGGGTGGAATCGG60.12639561828523277No Hit
CTATTATCAGGCCTAGTTGGCTTGATGTAGAGAAGGCAATGATTTTTTGA60.12639561828523277No Hit
GTCCCCAATGGAGGAGTTAGAGAAAGGACCCAAGGAGCTGAAGGGGTTGC60.12639561828523277No Hit
TAATTACACTAATCGCCACTTCTATAACAGCTATGTACAGCATACGAATC60.12639561828523277No Hit
AATCATACCATTCACATCATCATGCCTAGTAATCGGAAGCCTCGCCCTCA60.12639561828523277No Hit
GTATATGCAGTGAATTCTTGCAGAGATTGGATGAGTGTTTTGGATTCTCT60.12639561828523277No Hit
TTCCTACACATCTGTACCCACGCATTCTTCAAAGCTATACTCTTTATATG60.12639561828523277No Hit
CAAATACAGAGGTGGATGTTCACAGCCAACCATTGTACTGAGCACAGGGT60.12639561828523277No Hit
GTATCAACGCAGAGTACATGGGTCCCTCCATGTGTACTCTTTGATTGGTG60.12639561828523277No Hit
CCACATTATTTACAGCTATTTGTGCTCTCACCCAAAACGACATCAAAAAA60.12639561828523277No Hit
CTCTATAACCGCATCGGAGACATCGGATTCATTTTAGCTATAGTTTGATT60.12639561828523277No Hit
CATATAATATTCCACCAACCAGCATTCCAGTCCTCACAATACCATGATTT50.10532968190436064No Hit
GTGCTCAGTACAATGGTTGGCTGTGAACATCCACCTCTGTATTTGTCAGG50.10532968190436064No Hit
TCTCATGAGTTTATGTTTAGGGAAAATCAAACTATAGCTAAAATGAATCC50.10532968190436064No Hit
CATCTAGAGACTGCCCCACCTGGGGATCCATCCTATATACAGTCACCAAA50.10532968190436064No Hit
CAGTACAATGGTTGGCTGTGAACATCCACCTCTGTATTTGTCAGGCACTG50.10532968190436064No Hit
ACGCAGAGTACATGGGTCCCTCCATGTGTACTCTTTGATTGGTGGTTTAG50.10532968190436064No Hit
AAGCTATACTCTTTATATGCTCTGGCTCAATCATTCATAGCCTGGCAGAC50.10532968190436064No Hit
GCATATAAAGAGTATAGCTTTGAAGAATGCGTGGGTACAGATGTGTAGGA50.10532968190436064No Hit
ATATACAGTCACCAAACCCAGACACTATTGTGAATGCCAACAAGTGCTTG50.10532968190436064No Hit
CTCATGAGAACTTCAACAGATTATATTCTCCAACAACAACGACAATCTAA50.10532968190436064No Hit
GCTTTGAAGAATGCGTGGGTACAGATGTGTAGGAATGCTAGGTGTGGTTG50.10532968190436064No Hit
CCCTAATCTCCATTAACGAAAATGACCCAGACCTCATAAACCCAATCAAA50.10532968190436064No Hit
CCCCAATGGAGGAGTTAGAGAAAGGACCCAAGGAGCTGAAGGGGTTGCAG50.10532968190436064No Hit
CTATGAGGCTGCAACCCCTTCAGCTCCTTGGGTCCTTTCTCTAACTCCTC50.10532968190436064No Hit
ATCCTACAGTCACAACTCTAATTCAGAATTGTTCCTGCCTGAAAGAACTT50.10532968190436064No Hit
CATAAACCCAATCAAACGCCTAGCATTCGGAAGCATCTTTGCAGGATTTG50.10532968190436064No Hit
GATGTAGAGAAGGCAATGATTTTTTGATGTCGTTTTGGGTGAGAGCACAA50.10532968190436064No Hit
ATCAACGCAGAGTACATGGGGTGGAGCAGAGACTGAAGGAATGATCATCT50.10532968190436064No Hit
TTACCATGCTTATCCTCACCTCAGCCAACAACATATTTCAACTTTTCATT50.10532968190436064No Hit
GCTATACTCTTTATATGCTCTGGCTCAATCATTCATAGCCTGGCAGACGA50.10532968190436064No Hit
ATGGTTGGCTGTGAACATCCACCTCTGTATTTGTCAGGCACTGGTAGAGC50.10532968190436064No Hit
ATTAATACCTGCAACACCAACGCCTGAGCCCTACTAATTACACTAATCGC50.10532968190436064No Hit
CTTCTATAACAGCTATGTACAGCATACGAATCATTTACTTCGTAACAATA50.10532968190436064No Hit
GTATCAACGCAGAGTACATGGGTGTAATGCGGTGAATAATAGATGGGAAA50.10532968190436064No Hit
CCCCTAATCTCCATTAACGAAAATGACCCAGACCTCATAAACCCAATCAA50.10532968190436064No Hit
GTCTTGTTCGTCTGCCAGGCTATGAATGATTGAGCCAGAGCATATAAAGA50.10532968190436064No Hit
ATTCCTAACAGGGTTCTACTCAAAAGACCTAATTATTGAAGCAATTAATA50.10532968190436064No Hit
CCTACAAGCAATCCTCTATAACCGCATCGGAGACATCGGATTCATTTTAG50.10532968190436064No Hit
CCATTGGGGACCCTGTGCTCAGTACAATGGTTGGCTGTGAACATCCACCT50.10532968190436064No Hit
ATAATAGATGGGAAAAACCCCAGTAAAGTTGAGAAGGATGAATATGGATT50.10532968190436064No Hit
GTGTAGTAGTGCTGAAACTGGTGTAGGGCCTTCTATTGCTGATGGTAGTC50.10532968190436064No Hit
TTCGTATGCTGTACATAGCTGTTATAGAAGTGGCGATTAGTGTAATTAGT50.10532968190436064No Hit
CTCCTGAGAGGCTCTACCAGTGCCTGACAAATACAGAGGTGGATGTTCAC50.10532968190436064No Hit
CACCTAGCATTCCTACACATCTGTACCCACGCATTCTTCAAAGCTATACT50.10532968190436064No Hit
GAGTATAGCTTTGAAGAATGCGTGGGTACAGATGTGTAGGAATGCTAGGT50.10532968190436064No Hit
CTACTAATTACACTAATCGCCACTTCTATAACAGCTATGTACAGCATACG50.10532968190436064No Hit
AAATAAGGGGCTGGAGACATGGTTCAGTGATTGCACTGTCTTTCTTGCTG50.10532968190436064No Hit
CTTTTGAGTAGAACCCTGTTAGGAATGGTATTCCTGTGAGGGCGAGGCTT50.10532968190436064No Hit
ACTATACTTTGCCTCGGAGCCCTAACCACATTATTTACAGCTATTTGTGC50.10532968190436064No Hit
CCATAAAACTATCAATAAATAAAGCAAAATCCATATTCATCCTTCTCAAC50.10532968190436064No Hit
CTCAAAGGGAGGGACCTTAGATGAAATGCCCTACAGTTGAAAGAGGGAAC50.10532968190436064No Hit
ATTTGTGCTCTCACCCAAAACGACATCAAAAAATCATTGCCTTCTCTACA50.10532968190436064No Hit
CTTCAACAGATTATATTCTCCAACAACAACGACAATCTAATTCCACTTAT50.10532968190436064No Hit
AGTAAATATTATGTAAAACCACGACCTTTTAAAACAAAATAAGGGGCTGG50.10532968190436064No Hit
AATCAGCACAATTTGGCCTCCACCCATGACTACCATCAGCAATAGAAGGC50.10532968190436064No Hit
ATATTCTCCAACAACAACGACAATCTAATTCCACTTATAGGCCTATTAAT50.10532968190436064No Hit
GAGTTACAGATGGCTCTAAGTCCCCATCTGTGAGTGCTGGGAATCAAATC50.10532968190436064No Hit
CTCCATTTCCATCCCTGAAGTTCTTTCAGGCAGGAACAATTCTGAATTAG50.10532968190436064No Hit
GTATAATTGATCATGTGACAAAAAGGGCTACAGATGTAAACAGGATAGAG50.10532968190436064No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers