FastQCFastQC Report
Thu 2 Feb 2017
SRR4063884_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063884_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences370470
Sequences flagged as poor quality0
Sequence length25
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATGTACTCTGCGTTGATACCAC23730.6405376953599482No Hit
GTACATGGAAGCAGTGGTATCAACG23510.6345992927902394No Hit
GTATCAACGCAGAGTACTTTTTTTT20530.5541609307096391No Hit
GTACTTTTTTTTTTTTTTTTTTTTT18500.4993656706345993No Hit
GTACTGGGGTGAGTCGATCCTAAGC17090.4613059087105569No Hit
TATCAACGCAGAGTACTTTTTTTTT16070.4337733149782709No Hit
CCCCAGTACCATCGCGGTAAACCAG14450.3900450778740519No Hit
GAGTACTTTTTTTTTTTTTTTTTTT12090.32634221394444896No Hit
ACCCAGGGGAAGGTGCATTCGGACC11660.3147353361945637No Hit
GTATCAACGCAGAGTACATGGGCAG11620.3136556266364348No Hit
GTTTTAATTAGACAGTCAGATTCCC11250.3036683132237428No Hit
ACGCAGAGTACTTTTTTTTTTTTTT10240.276405646880989No Hit
GTTCTCTTGTTGTCGTAATTGTGGC10090.2723567360380058No Hit
GTACATGGGGTGGTATCAACGCAAA9790.2642589143520393No Hit
GTCGTACTGGAATCCGCAACAGGTC9710.2620994952357816No Hit
CTCTTAAGGTAGCCAAATGCCTCGT9520.2569708748346695No Hit
CCCCTTGTCCGTGCCAGTTCTGAGT9200.24833319836963857No Hit
CCCATGTACTCTGCGTTGATACCAC9020.2434745053580587No Hit
GTACATGGGCAGAAATCACATCGCG8970.24212486841039763No Hit
CCCTTACCCACATTCTTCCATTCGA8960.24185494102086538No Hit
GTACATGGGAAGCAGTGGTATCAAC8760.23645639323022108No Hit
CATGTACTCTGCGTTGATACCACTG8740.23591653845115665No Hit
GAGTACATGGAAGCAGTGGTATCAA8410.2270089345965935No Hit
CCTTAAGAGAGTCATAGTTACTCCC8180.22080060463735254No Hit
CCTCAGAGCCACCCCTTATCCCAAA7740.20892379949793505No Hit
GGGTAAGGGAAGTCGGCAAGCCAGC7610.20541474343401625No Hit
TATCAACGCAGAGTACATGGGCAGA7440.2008259778119686No Hit
CCCCTGGGTCACGCGCGTTGCCGCC7260.19596728480038872No Hit
GGTATCAACGCAGAGTACTTTTTTT7240.19542743002132426No Hit
CGTATTATCCGTGTCCTGCGGCACT7220.19488757524225983No Hit
ATCCTAAGCGGGGGGAGAAGTCCCT7130.1924582287364699No Hit
GATAATACGCGCCCGTGGCACAGCG6890.18597997138769673No Hit
CCCCTATGCTGCACCCAGTGGGCCG6680.18031149620752018No Hit
GTACTGGAATCCGCAACAGGTCTCC6560.17707236753313357No Hit
ATACACGACCGACGGCCTCAGACGG6520.1759926579750047No Hit
GTCAACAGCCAACTCAGAACTGGCA6350.17140389235295705No Hit
GCTTCCATGTACTCTGCGTTGATAC6290.16978432801576376No Hit
GTACATGGGTGTCCGAAGACTCCCC6250.1687046184576349No Hit
GTCAGATTCCCCTTGTCCGTGCCAG6250.1687046184576349No Hit
GAGCCACCCCTTATCCCAAAGTTAC6170.16654519934137718No Hit
GCTAAGAAGTGTGTAACAACTTACC5830.15736766809728184No Hit
GACAAGGGGAATCTGACTGTCTAAT5810.1568278133182174No Hit
CCCCTACACGACGACCGTCTGAGGC5800.1565578859286852No Hit
CAAGAGAACTTGCCCCCGGTCCGAA5760.15547817637055633No Hit
ACGCAGAGTACATGGGCAGAAATCA5740.15493832159149187No Hit
GTCCGCAAGGAGCGCTGCTTGTCCA5680.1533187572542986No Hit
TATTATCCGTGTCCTGCGGCACTGT5620.1516991929171053No Hit
GTCGTAATTGTGGCCATGCCTCGGA5590.15088941074850865No Hit
ATGCTATGTTTTAATTAGACAGTCA5480.14792020946365428No Hit
CCCTCGGGGGTCGCTGTGCCACGGG5440.14684049990552542No Hit
CTTCTTAGCGGATGCCGACTTCCAT5430.1465705725159932No Hit
CATGGAAGCAGTGGTATCAACGCAG5410.14603071773692877No Hit
TCCGTGGAGACCATCGCGATGCTAT5410.14603071773692877No Hit
GGTTAGCAGCCTCTAGTCGAATGGA5410.14603071773692877No Hit
CTTGAAAACCCTGGTGATCATTTGG5380.14522093556833213No Hit
ACTAGAGGCTGCTAACCTAGGAGAC5370.1449510081787999No Hit
GTATCAACGCAGAGTACATGGGAAG5340.14414122601020327No Hit
GTATCAACGCAGAGTACATGGGGTG5270.14225173428347773No Hit
TTCCAGTACGACAGTGAACGCGAGG5260.14198180689394552No Hit
GTTCTGAGTTGGCTGTTGACAGTGC5210.14063216994628444No Hit
GTACATGGGTGGTATCAACGCAAAA5120.1382028234404945No Hit
GTGTAGGGGGGCGTGGAGGTCCTGC5060.1365832591033012No Hit
AATTAAAACATAGCATCGCGATGGT5010.13523362215564014No Hit
ACTCAGAACTGGCACGGACAAGGGG5010.13523362215564014No Hit
CTATTAGGGAACGTGAGCTGGGTTT4980.1344238399870435No Hit
GCAGAGTACTTTTTTTTTTTTTTTT4960.13388398520797906No Hit
GCTTAGGATCGACTCACCCCAGTAC4900.13226442087078577No Hit
CAATTACGACAACAAGAGAACTTGC4800.12956514697546362No Hit
GCTATGTTTTAATTAGACAGTCAGA4790.12929521958593138No Hit
GCGTTGATACCACTGCTTCCATGTA4760.12848543741733476No Hit
GTCTTCACGGTTCGCACGAGAATAA4740.1279455826382703No Hit
CATCCGTGGAGACCATCGCGATGCT4730.12767565524873808No Hit
TCCGGAGAGGGGGCAAGGTCCGCAA4610.1244365265743515No Hit
GCCTCTAGTCGAATGGAAGAATGTG4520.12200718006856155No Hit
GTTACGGAGCTGGCTTGCCGACTTC4510.12173725267902935No Hit
GTATCAACGCAGAGTACATGGAAGC4480.12092747051043269No Hit
GTACATGGGAGTGGTATCAACGCAA4470.12065754312090048No Hit
AAATCACATCGCGTCAACATCCGTG4410.1190379787837072No Hit
AGCCAGCTCCGTAACTTTGGGATAA4400.11876805139417496No Hit
GTGGAGACCATCGCGATGCTATGTT4350.11741841444651388No Hit
ATGCTGCACCCAGTGGGCCGCCTCA4300.1160687774988528No Hit
CCTAGGAGACCTGTTGCGGATTCCA4290.1157988501093206No Hit
GTATTATCCGTGTCCTGCGGCACTG4290.1157988501093206No Hit
GGTATCAACGCAGAGTACATGGGCA4290.1157988501093206No Hit
ATACGCGCCCGTGGCACAGCGACCC4270.11525899533025616No Hit
CGGATAATACGCGCCCGTGGCACAG4250.11471914055119173No Hit
GCTCTGAGGGCTGGGTGTCGTGGGT4250.11471914055119173No Hit
CAGTACGACAGTGAACGCGAGGGTG4250.11471914055119173No Hit
CTGCTAACCTAGGAGACCTGTTGCG4250.11471914055119173No Hit
CCCCTTATCCCAAAGTTACGGAGCT4240.11444921316165953No Hit
GTCGTGTATCGGTCGCTCATGCTTC4140.11174993926633735No Hit
TCCGTGTCCTGCGGCACTGTCAACA4140.11174993926633735No Hit
GAGCTGGCTTGCCGACTTCCCTTAC4100.11067022970820849No Hit
GTCCCTATCCACCATCCAGCGAAAC4030.10878073798148298No Hit
ACTCTGCGTTGATACCACTGCTTCC4000.10797095581288634No Hit
CTCCTAGGTTAGCAGCCTCTAGTCG4000.10797095581288634No Hit
CCCTAATAGCGGGTGAACAATCCGA3980.1074311010338219No Hit
GCACTAGAGGCCGCTCCAGCCTGGC3960.10689124625475746No Hit
GGGGTGAGTCGATCCTAAGCGGGGG3930.10608146408616081No Hit
TCTCCTAGGTTAGCAGCCTCTAGTC3890.10500175452803197No Hit
CTGTCGTACTGGAATCCGCAACAGG3880.10473182713849974No Hit
CCTTAACACAACGTTCGGTTGATCC3840.10365211758037088No Hit
ATCCCAAAGTTACGGAGCTGGCTTG3820.10311226280130645No Hit
TATCAACGCAGAGTACATGGGAAGC3810.10284233541177423No Hit
GGCGCGTATTATCCGTGTCCTGCGG3810.10284233541177423No Hit
ATCCAGGCCCGTTCCCCTGGCTGTG3730.10068291629551651No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACTC1400.016.96971316
AGACTCC1400.016.96742217
CAAACGC402.7529514E-416.62582813
CGAAGAC1450.016.38012514
CCTAATA702.4834662E-816.2865242
TTTGAGA350.002165283316.2865242
CAAGAGT350.002169194216.2821274
CTAATAG702.4916517E-816.2821273
GACTCCC1500.015.8362618
CCGAAGA1500.015.83412213
CTAAGAA1150.015.6964342
TCCGAAG1550.015.32334212
AGCTGGG755.781294E-815.20486416
CCCTAAT755.7909347E-815.2028091
AAACGCA508.681718E-515.20075714
AGCGGGT755.8198566E-815.1966518
CCCTCGG1200.015.0444461
CCTCGGG1200.015.0424162
AGAAGTG1400.014.92528255
GGTGTCC1600.014.840488
Produced by FastQC (version 0.11.5)