##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063884_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	370470
Sequences flagged as poor quality	0
Sequence length	25
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.05487083974411	32.0	32.0	32.0	21.0	32.0
2	28.463638081356116	32.0	32.0	32.0	14.0	32.0
3	28.693397576052043	32.0	32.0	32.0	14.0	32.0
4	28.61830917483197	32.0	32.0	32.0	14.0	32.0
5	28.633365724620077	32.0	32.0	32.0	14.0	32.0
6	32.05288417415715	36.0	32.0	36.0	21.0	36.0
7	31.845215537020543	36.0	32.0	36.0	21.0	36.0
8	31.75731098334548	36.0	32.0	36.0	21.0	36.0
9	32.06400248333198	36.0	32.0	36.0	21.0	36.0
10	30.83826220746619	36.0	32.0	36.0	14.0	36.0
11	32.24292115420952	36.0	32.0	36.0	21.0	36.0
12	31.461457068048695	36.0	32.0	36.0	14.0	36.0
13	31.973050449429103	36.0	32.0	36.0	21.0	36.0
14	31.520106891246254	36.0	32.0	36.0	14.0	36.0
15	31.525105946500393	36.0	32.0	36.0	14.0	36.0
16	31.369438820957164	36.0	32.0	36.0	14.0	36.0
17	31.340445920047507	36.0	32.0	36.0	14.0	36.0
18	31.356101708640377	36.0	32.0	36.0	14.0	36.0
19	31.254787162253354	36.0	32.0	36.0	14.0	36.0
20	31.390781979647475	36.0	32.0	36.0	14.0	36.0
21	31.245650120117688	36.0	32.0	36.0	14.0	36.0
22	31.080324992576998	36.0	32.0	36.0	14.0	36.0
23	30.946886387561747	36.0	32.0	36.0	14.0	36.0
24	30.891675439306827	36.0	32.0	36.0	14.0	36.0
25	29.94600642427187	36.0	21.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	2.0
4	7.0
5	4.0
6	13.0
7	10.0
8	24.0
9	17.0
10	28.0
11	12.0
12	18.0
13	16.0
14	337.0
15	720.0
16	947.0
17	1147.0
18	1609.0
19	2136.0
20	3019.0
21	4042.0
22	5353.0
23	6705.0
24	8426.0
25	10510.0
26	13283.0
27	16091.0
28	20213.0
29	24185.0
30	29190.0
31	35876.0
32	43796.0
33	48511.0
34	58355.0
35	35868.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.752350504663205	16.697358686201554	11.590512655732747	25.959778153402496
2	17.082026336106598	19.28615005857867	35.91088483487294	27.720938770441794
3	19.959239863952924	25.50666738649247	25.801436052475303	28.732656697079307
4	14.541847187917561	14.692202831652967	32.2943407431402	38.47160923728928
5	14.944160846995052	36.76988654002413	31.449982318276426	16.835970294704392
6	32.45461294459067	33.486670716069376	17.38408584733752	16.67463049200243
7	30.366418643829846	29.78136912925626	20.039363276061298	19.8128489508526
8	31.97183212682023	28.080388604324007	18.74492035933479	21.20285890952097
9	31.610741154803762	12.816960741424847	17.38254562344389	38.189752480327506
10	18.825264192676332	24.567150716081656	28.025623921421417	28.581961169820598
11	37.404670857908265	23.179024348089097	18.934582064078466	20.48172272992417
12	25.761193062434845	24.209369277886104	26.58031619827479	23.449121461404257
13	32.50376748029837	18.412310883291834	23.324078925336373	25.75984271107342
14	25.89459582823544	17.403599180015718	24.919311494094547	31.782493497654297
15	28.65341352554979	22.432665274915806	22.80076807363136	26.11315312590304
16	24.62524915866748	25.966519557267333	23.78310636711808	25.62512491694711
17	25.381200071297783	24.22288118656793	23.71974570457872	26.676173037555568
18	25.780268755486528	24.402728070767775	27.034100884597205	22.782902289148492
19	26.184193111867998	24.03742528313404	24.061464329027014	25.716917275970946
20	28.114178906937926	21.471479582741352	25.201903702063067	25.21243780825766
21	28.565177580241315	22.44460830787598	24.079821298824776	24.910392813057936
22	26.07326335805697	23.394899331759518	23.727669045393895	26.804168264789613
23	26.31955680286533	23.03825573511894	24.874736304727744	25.767451157287987
24	26.45141075991421	24.39239815680152	22.326632921512807	26.82955816177146
25	26.670718202198636	23.24744078005564	22.77665235124112	27.305188666504606
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	144.0
1	144.0
2	333.0
3	522.0
4	522.0
5	522.0
6	1288.0
7	2054.0
8	2054.0
9	2054.0
10	1807.5
11	1561.0
12	1561.0
13	1561.0
14	1007.0
15	453.0
16	453.0
17	453.0
18	887.0
19	1321.0
20	1321.0
21	1321.0
22	1966.0
23	2611.0
24	2611.0
25	2611.0
26	4510.0
27	6409.0
28	6409.0
29	6409.0
30	7345.0
31	8281.0
32	8281.0
33	8281.0
34	10967.5
35	13654.0
36	13654.0
37	13654.0
38	16065.5
39	18477.0
40	18477.0
41	18477.0
42	23532.0
43	28587.0
44	28587.0
45	28587.0
46	42400.0
47	56213.0
48	56213.0
49	56213.0
50	56978.5
51	57744.0
52	57744.0
53	57744.0
54	56096.5
55	54449.0
56	54449.0
57	54449.0
58	48075.0
59	41701.0
60	41701.0
61	41701.0
62	36750.0
63	31799.0
64	31799.0
65	31799.0
66	26617.0
67	21435.0
68	21435.0
69	21435.0
70	16914.5
71	12394.0
72	12394.0
73	12394.0
74	9002.0
75	5610.0
76	5610.0
77	5610.0
78	4864.5
79	4119.0
80	4119.0
81	4119.0
82	2381.5
83	644.0
84	644.0
85	644.0
86	355.0
87	66.0
88	66.0
89	66.0
90	44.0
91	22.0
92	22.0
93	22.0
94	20.5
95	19.0
96	19.0
97	19.0
98	100.0
99	181.0
100	181.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.033470996301994765
2	0.007557966906902043
3	0.0026992738953221583
4	0.004048910842983238
5	0.008367749075498691
6	0.012146732528949713
7	0.019974626825383972
8	0.03212135935433369
9	0.04210867276702567
10	0.051556131400653224
11	0.04696736577860555
12	0.0529057683483143
13	0.0529057683483143
14	0.05911409830755527
15	0.052095986179717654
16	0.060463735255216346
17	0.05182605879018544
18	0.06613221043539289
19	0.06451264609819958
20	0.06586228304586066
21	0.06559235565632845
22	0.06586228304586066
23	0.06775177477258618
24	0.06586228304586066
25	0.06478257348773181
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	370470.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.700442460339445
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.40856291229424	20.057492305878757
2	14.614100497194773	8.096340998643239
3	4.862522824721977	4.0408210115489505
4	2.1654292682971663	2.3993339539360243
5	1.21684307294573	1.6853545762697917
6	0.7326914400319762	1.2177526245473402
7	0.47315427607923477	0.9174607959577483
8	0.35137189656431983	0.7786525602368227
9	0.24955389955123863	0.6221478091745174
>10	2.0173937198382776	11.721817720941734
>50	0.389304082491863	7.55370391811183
>100	0.4551863118366398	25.973979703778998
>500	0.050908995402782084	9.38198894287775
>1k	0.012976802749728768	5.553153078096488
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCCATGTACTCTGCGTTGATACCAC	2373	0.6405376953599482	No Hit
GTACATGGAAGCAGTGGTATCAACG	2351	0.6345992927902394	No Hit
GTATCAACGCAGAGTACTTTTTTTT	2053	0.5541609307096391	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	1850	0.4993656706345993	No Hit
GTACTGGGGTGAGTCGATCCTAAGC	1709	0.4613059087105569	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1607	0.4337733149782709	No Hit
CCCCAGTACCATCGCGGTAAACCAG	1445	0.3900450778740519	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	1209	0.32634221394444896	No Hit
ACCCAGGGGAAGGTGCATTCGGACC	1166	0.3147353361945637	No Hit
GTATCAACGCAGAGTACATGGGCAG	1162	0.3136556266364348	No Hit
GTTTTAATTAGACAGTCAGATTCCC	1125	0.3036683132237428	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1024	0.276405646880989	No Hit
GTTCTCTTGTTGTCGTAATTGTGGC	1009	0.2723567360380058	No Hit
GTACATGGGGTGGTATCAACGCAAA	979	0.2642589143520393	No Hit
GTCGTACTGGAATCCGCAACAGGTC	971	0.2620994952357816	No Hit
CTCTTAAGGTAGCCAAATGCCTCGT	952	0.2569708748346695	No Hit
CCCCTTGTCCGTGCCAGTTCTGAGT	920	0.24833319836963857	No Hit
CCCATGTACTCTGCGTTGATACCAC	902	0.2434745053580587	No Hit
GTACATGGGCAGAAATCACATCGCG	897	0.24212486841039763	No Hit
CCCTTACCCACATTCTTCCATTCGA	896	0.24185494102086538	No Hit
GTACATGGGAAGCAGTGGTATCAAC	876	0.23645639323022108	No Hit
CATGTACTCTGCGTTGATACCACTG	874	0.23591653845115665	No Hit
GAGTACATGGAAGCAGTGGTATCAA	841	0.2270089345965935	No Hit
CCTTAAGAGAGTCATAGTTACTCCC	818	0.22080060463735254	No Hit
CCTCAGAGCCACCCCTTATCCCAAA	774	0.20892379949793505	No Hit
GGGTAAGGGAAGTCGGCAAGCCAGC	761	0.20541474343401625	No Hit
TATCAACGCAGAGTACATGGGCAGA	744	0.2008259778119686	No Hit
CCCCTGGGTCACGCGCGTTGCCGCC	726	0.19596728480038872	No Hit
GGTATCAACGCAGAGTACTTTTTTT	724	0.19542743002132426	No Hit
CGTATTATCCGTGTCCTGCGGCACT	722	0.19488757524225983	No Hit
ATCCTAAGCGGGGGGAGAAGTCCCT	713	0.1924582287364699	No Hit
GATAATACGCGCCCGTGGCACAGCG	689	0.18597997138769673	No Hit
CCCCTATGCTGCACCCAGTGGGCCG	668	0.18031149620752018	No Hit
GTACTGGAATCCGCAACAGGTCTCC	656	0.17707236753313357	No Hit
ATACACGACCGACGGCCTCAGACGG	652	0.1759926579750047	No Hit
GTCAACAGCCAACTCAGAACTGGCA	635	0.17140389235295705	No Hit
GCTTCCATGTACTCTGCGTTGATAC	629	0.16978432801576376	No Hit
GTACATGGGTGTCCGAAGACTCCCC	625	0.1687046184576349	No Hit
GTCAGATTCCCCTTGTCCGTGCCAG	625	0.1687046184576349	No Hit
GAGCCACCCCTTATCCCAAAGTTAC	617	0.16654519934137718	No Hit
GCTAAGAAGTGTGTAACAACTTACC	583	0.15736766809728184	No Hit
GACAAGGGGAATCTGACTGTCTAAT	581	0.1568278133182174	No Hit
CCCCTACACGACGACCGTCTGAGGC	580	0.1565578859286852	No Hit
CAAGAGAACTTGCCCCCGGTCCGAA	576	0.15547817637055633	No Hit
ACGCAGAGTACATGGGCAGAAATCA	574	0.15493832159149187	No Hit
GTCCGCAAGGAGCGCTGCTTGTCCA	568	0.1533187572542986	No Hit
TATTATCCGTGTCCTGCGGCACTGT	562	0.1516991929171053	No Hit
GTCGTAATTGTGGCCATGCCTCGGA	559	0.15088941074850865	No Hit
ATGCTATGTTTTAATTAGACAGTCA	548	0.14792020946365428	No Hit
CCCTCGGGGGTCGCTGTGCCACGGG	544	0.14684049990552542	No Hit
CTTCTTAGCGGATGCCGACTTCCAT	543	0.1465705725159932	No Hit
CATGGAAGCAGTGGTATCAACGCAG	541	0.14603071773692877	No Hit
TCCGTGGAGACCATCGCGATGCTAT	541	0.14603071773692877	No Hit
GGTTAGCAGCCTCTAGTCGAATGGA	541	0.14603071773692877	No Hit
CTTGAAAACCCTGGTGATCATTTGG	538	0.14522093556833213	No Hit
ACTAGAGGCTGCTAACCTAGGAGAC	537	0.1449510081787999	No Hit
GTATCAACGCAGAGTACATGGGAAG	534	0.14414122601020327	No Hit
GTATCAACGCAGAGTACATGGGGTG	527	0.14225173428347773	No Hit
TTCCAGTACGACAGTGAACGCGAGG	526	0.14198180689394552	No Hit
GTTCTGAGTTGGCTGTTGACAGTGC	521	0.14063216994628444	No Hit
GTACATGGGTGGTATCAACGCAAAA	512	0.1382028234404945	No Hit
GTGTAGGGGGGCGTGGAGGTCCTGC	506	0.1365832591033012	No Hit
AATTAAAACATAGCATCGCGATGGT	501	0.13523362215564014	No Hit
ACTCAGAACTGGCACGGACAAGGGG	501	0.13523362215564014	No Hit
CTATTAGGGAACGTGAGCTGGGTTT	498	0.1344238399870435	No Hit
GCAGAGTACTTTTTTTTTTTTTTTT	496	0.13388398520797906	No Hit
GCTTAGGATCGACTCACCCCAGTAC	490	0.13226442087078577	No Hit
CAATTACGACAACAAGAGAACTTGC	480	0.12956514697546362	No Hit
GCTATGTTTTAATTAGACAGTCAGA	479	0.12929521958593138	No Hit
GCGTTGATACCACTGCTTCCATGTA	476	0.12848543741733476	No Hit
GTCTTCACGGTTCGCACGAGAATAA	474	0.1279455826382703	No Hit
CATCCGTGGAGACCATCGCGATGCT	473	0.12767565524873808	No Hit
TCCGGAGAGGGGGCAAGGTCCGCAA	461	0.1244365265743515	No Hit
GCCTCTAGTCGAATGGAAGAATGTG	452	0.12200718006856155	No Hit
GTTACGGAGCTGGCTTGCCGACTTC	451	0.12173725267902935	No Hit
GTATCAACGCAGAGTACATGGAAGC	448	0.12092747051043269	No Hit
GTACATGGGAGTGGTATCAACGCAA	447	0.12065754312090048	No Hit
AAATCACATCGCGTCAACATCCGTG	441	0.1190379787837072	No Hit
AGCCAGCTCCGTAACTTTGGGATAA	440	0.11876805139417496	No Hit
GTGGAGACCATCGCGATGCTATGTT	435	0.11741841444651388	No Hit
ATGCTGCACCCAGTGGGCCGCCTCA	430	0.1160687774988528	No Hit
CCTAGGAGACCTGTTGCGGATTCCA	429	0.1157988501093206	No Hit
GTATTATCCGTGTCCTGCGGCACTG	429	0.1157988501093206	No Hit
GGTATCAACGCAGAGTACATGGGCA	429	0.1157988501093206	No Hit
ATACGCGCCCGTGGCACAGCGACCC	427	0.11525899533025616	No Hit
CGGATAATACGCGCCCGTGGCACAG	425	0.11471914055119173	No Hit
GCTCTGAGGGCTGGGTGTCGTGGGT	425	0.11471914055119173	No Hit
CAGTACGACAGTGAACGCGAGGGTG	425	0.11471914055119173	No Hit
CTGCTAACCTAGGAGACCTGTTGCG	425	0.11471914055119173	No Hit
CCCCTTATCCCAAAGTTACGGAGCT	424	0.11444921316165953	No Hit
GTCGTGTATCGGTCGCTCATGCTTC	414	0.11174993926633735	No Hit
TCCGTGTCCTGCGGCACTGTCAACA	414	0.11174993926633735	No Hit
GAGCTGGCTTGCCGACTTCCCTTAC	410	0.11067022970820849	No Hit
GTCCCTATCCACCATCCAGCGAAAC	403	0.10878073798148298	No Hit
ACTCTGCGTTGATACCACTGCTTCC	400	0.10797095581288634	No Hit
CTCCTAGGTTAGCAGCCTCTAGTCG	400	0.10797095581288634	No Hit
CCCTAATAGCGGGTGAACAATCCGA	398	0.1074311010338219	No Hit
GCACTAGAGGCCGCTCCAGCCTGGC	396	0.10689124625475746	No Hit
GGGGTGAGTCGATCCTAAGCGGGGG	393	0.10608146408616081	No Hit
TCTCCTAGGTTAGCAGCCTCTAGTC	389	0.10500175452803197	No Hit
CTGTCGTACTGGAATCCGCAACAGG	388	0.10473182713849974	No Hit
CCTTAACACAACGTTCGGTTGATCC	384	0.10365211758037088	No Hit
ATCCCAAAGTTACGGAGCTGGCTTG	382	0.10311226280130645	No Hit
TATCAACGCAGAGTACATGGGAAGC	381	0.10284233541177423	No Hit
GGCGCGTATTATCCGTGTCCTGCGG	381	0.10284233541177423	No Hit
ATCCAGGCCCGTTCCCCTGGCTGTG	373	0.10068291629551651	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGACTC	140	0.0	16.969713	16
AGACTCC	140	0.0	16.967422	17
CAAACGC	40	2.7529514E-4	16.625828	13
CGAAGAC	145	0.0	16.380125	14
CCTAATA	70	2.4834662E-8	16.286524	2
TTTGAGA	35	0.0021652833	16.286524	2
CAAGAGT	35	0.0021691942	16.282127	4
CTAATAG	70	2.4916517E-8	16.282127	3
GACTCCC	150	0.0	15.83626	18
CCGAAGA	150	0.0	15.834122	13
CTAAGAA	115	0.0	15.696434	2
TCCGAAG	155	0.0	15.323342	12
AGCTGGG	75	5.781294E-8	15.204864	16
CCCTAAT	75	5.7909347E-8	15.202809	1
AAACGCA	50	8.681718E-5	15.200757	14
AGCGGGT	75	5.8198566E-8	15.196651	8
CCCTCGG	120	0.0	15.044446	1
CCTCGGG	120	0.0	15.042416	2
AGAAGTG	140	0.0	14.9252825	5
GGTGTCC	160	0.0	14.84048	8
>>END_MODULE