FastQCFastQC Report
Thu 2 Feb 2017
SRR4063884_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063884_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences370470
Sequences flagged as poor quality0
Sequence length50
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA14530.39220449699030957No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC12930.34901611466515503No Hit
CCCCAGTACCATCGCGGTAAACCAGGAACCTTTCCCCACATCAGTCTTCA11300.3050179501714039No Hit
GTATCAACGCAGAGTACATGGGCAGAAATCACATCGCGTCAACATCCGTG9360.252652036602154No Hit
ACCCAGGGGAAGGTGCATTCGGACCGGGGGCAAGTTCTCTTGTTGTCGTA8960.24185494102086538No Hit
GTACTGGGGTGAGTCGATCCTAAGCGGGGGGAGAAGTCCCTTTCAGACAC8780.2369962480092855No Hit
GTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTGCCAGTTCTGAGTTGG8400.2267390072070613No Hit
ATACACGACCGACGGCCTCAGACGGTCGTCGTGTAGGGGGGCGTGGAGGT7920.21378249250951492No Hit
CCTTAAGAGAGTCATAGTTACTCCCGCCGTTAACCCGCGCTTGGTTGAAT7920.21378249250951492No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA7260.19596728480038872No Hit
GATAATACGCGCCCGTGGCACAGCGACCCCCGAGGGAGCCCGCGACGCAC7260.19596728480038872No Hit
CCCTTACCCACATTCTTCCATTCGACTAGAGGCTGCTAACCTAGGAGACC6910.18651982616676113No Hit
CGTATTATCCGTGTCCTGCGGCACTGTCAACAGCCAACTCAGAACTGGCA6690.1805814235970524No Hit
TATCAACGCAGAGTACATGGGCAGAAATCACATCGCGTCAACATCCGTGG6510.1757227305854725No Hit
CTCTTAAGGTAGCCAAATGCCTCGTCATCTAATTAGTGACGCGCATGAAT6460.17437309363781142No Hit
GTACATGGGCAGAAATCACATCGCGTCAACATCCGTGGAGACCATCGCGA6370.17194374713202148No Hit
CCTCAGAGCCACCCCTTATCCCAAAGTTACGGAGCTGGCTTGCCGACTTC6320.1705941101843604No Hit
CCCCTATGCTGCACCCAGTGGGCCGCCTCACATACCAAACTAGAGTCAAG6310.1703241827948282No Hit
GTCGTACTGGAATCCGCAACAGGTCTCCTAGGTTAGCAGCCTCTAGTCGA6230.16816476367857044No Hit
GACAAGGGGAATCTGACTGTCTAATTAAAACATAGCATCGCGATGGTCTC6190.1670850541204416No Hit
ACTAGAGGCTGCTAACCTAGGAGACCTGTTGCGGATTCCAGTACGACAGT6180.16681512673090937No Hit
AATTAAAACATAGCATCGCGATGGTCTCCACGGATGTTGACGCGATGTGA6070.16384592544605503No Hit
GGGTAAGGGAAGTCGGCAAGCCAGCTCCGTAACTTTGGGATAAGGGGTGG6010.1622263611088617No Hit
CCCCTTGTCCGTGCCAGTTCTGAGTTGGCTGTTGACAGTGCCGCAGGACA5950.16060679677166842No Hit
GAGCCACCCCTTATCCCAAAGTTACGGAGCTGGCTTGCCGACTTCCCTTA5850.15790752287634627No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5810.1568278133182174No Hit
TATTATCCGTGTCCTGCGGCACTGTCAACAGCCAACTCAGAACTGGCACG5810.1568278133182174No Hit
GTCAGATTCCCCTTGTCCGTGCCAGTTCTGAGTTGGCTGTTGACAGTGCC5620.1516991929171053No Hit
CCCCTGGGTCACGCGCGTTGCCGCCCAAATCACCCTGCCACTGTAGCCGA5580.15061948335897643No Hit
GTTCTCTTGTTGTCGTAATTGTGGCCATGCCTCGGAAACGGGTCGTCCGG5460.14738035468458985No Hit
ACGCAGAGTACATGGGCAGAAATCACATCGCGTCAACATCCGTGGAGACC5380.14522093556833213No Hit
AAATCACATCGCGTCAACATCCGTGGAGACCATCGCGATGCTATGTTTTA5370.1449510081787999No Hit
GCTAAGAAGTGTGTAACAACTTACCTGCCGAATCGCTCAGCCCTGAAAAT5320.1436013712311388No Hit
TTCCAGTACGACAGTGAACGCGAGGGTGCCAAATGATCACCAGGGTTTTC5130.13847275083002672No Hit
CAAGAGAACTTGCCCCCGGTCCGAATGCACCTTCCCCTGGGTCACGCGCG5100.13766296866143007No Hit
GTCAACAGCCAACTCAGAACTGGCACGGACAAGGGGAATCTGACTGTCTA4970.13415391259751125No Hit
ATCCTAAGCGGGGGGAGAAGTCCCTTTCAGACACGGCGCCTCTGCGCGTC4960.13388398520797906No Hit
GTACTGGAATCCGCAACAGGTCTCCTAGGTTAGCAGCCTCTAGTCGAATG4890.13199449348125356No Hit
CCTAGGAGACCTGTTGCGGATTCCAGTACGACAGTGAACGCGAGGGTGCC4850.1309147839231247No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA4760.12848543741733476No Hit
CAGTACGACAGTGAACGCGAGGGTGCCAAATGATCACCAGGGTTTTCAAG4740.1279455826382703No Hit
TCCGTGGAGACCATCGCGATGCTATGTTTTAATTAGACAGTCAGATTCCC4730.12767565524873808No Hit
GTACATGGGTGTCCGAAGACTCCCCCCTATGCTGCACCCAGTGGGCCGCC4680.126326018301077No Hit
ACTCAGAACTGGCACGGACAAGGGGAATCTGACTGTCTAATTAAAACATA4630.12497638135341593No Hit
GTCCGCAAGGAGCGCTGCTTGTCCAGCGTCTCGAGCTTCGGGTGCATCCC4600.12416659918481929No Hit
ATACGCGCCCGTGGCACAGCGACCCCCGAGGGAGCCCGCGACGCACGAGC4560.12308688962669043No Hit
GTTACGGAGCTGGCTTGCCGACTTCCCTTACCCACATTCTTCCATTCGAC4470.12065754312090048No Hit
TCCGGAGAGGGGGCAAGGTCCGCAAGGAGCGCTGCTTGTCCAGCGTCTCG4430.11957783356277162No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC4400.11876805139417496No Hit
GGTTAGCAGCCTCTAGTCGAATGGAAGAATGTGGGTAAGGGAAGTCGGCA4390.11849812400464275No Hit
ATGCTATGTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTGCCAGTTCT4350.11741841444651388No Hit
CATCCGTGGAGACCATCGCGATGCTATGTTTTAATTAGACAGTCAGATTC4330.11687855966744946No Hit
CTATTAGGGAACGTGAGCTGGGTTTAGACCGTCGTGAGACAGGTTAGTTT4330.11687855966744946No Hit
GTGTAGGGGGGCGTGGAGGTCCTGCGGCGAAGGCCGTGGCGTGAGCCAGG4290.1157988501093206No Hit
TAATTAGACAGTCAGATTCCCCTTGTCCGTGCCAGTTCTGAGTTGGCTGT4250.11471914055119173No Hit
TCCGTGTCCTGCGGCACTGTCAACAGCCAACTCAGAACTGGCACGGACAA4240.11444921316165953No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT4230.11417928577212728No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA4120.11121008448727293No Hit
GCTATGTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTGCCAGTTCTGA4100.11067022970820849No Hit
GTGGAGACCATCGCGATGCTATGTTTTAATTAGACAGTCAGATTCCCCTT4070.10986044753961186No Hit
ATGCTGCACCCAGTGGGCCGCCTCACATACCAAACTAGAGTCAAGCTCAA4070.10986044753961186No Hit
CCCTAATAGCGGGTGAACAATCCGACCCTTACCGAGTTCTGCCTCGGTAG4010.10824088320241854No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC3990.10770102842335412No Hit
GTTCTGAGTTGGCTGTTGACAGTGCCGCAGGACACGGATAATACGCGCCC3830.10338219019083866No Hit
CTTGAAAACCCTGGTGATCATTTGGCACCCTCGCGTTCACTGTCGTACTG3790.1023024806327098No Hit
ATCCCAAAGTTACGGAGCTGGCTTGCCGACTTCCCTTACCCACATTCTTC3770.10176262585364537No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATATGC454.063209E-529.3320733
CAGTTAA300.005741142729.33207316
AAGCTTA300.005741142729.3320736
AAGACTC1800.024.44339416
GTGTCCG1900.024.3147459
GGTGTCC2100.023.046638
AGACTCC1950.022.56618117
ATGAGGG602.868406E-421.99905416
GACTCCC2050.021.4653918
CCCTACA2100.020.9514832
ATGGGTG4850.020.8650845
TAGCTCA654.924714E-420.3068210
CATGGGT6200.020.2249374
CCTAAGG550.004478959419.999142
GAGAGTC2000.019.799157
TAGCCAA1800.019.55471610
TCCGAAG2250.019.55471412
GGGTGTG808.970106E-519.2491727
GGTAGCT808.970106E-519.2491728
GGTAGCC1950.019.1786638
Produced by FastQC (version 0.11.5)