##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063883_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8229
Sequences flagged as poor quality	0
Sequence length	25
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.36735933892332	32.0	32.0	32.0	32.0	32.0
2	30.983837647344757	32.0	32.0	32.0	32.0	32.0
3	30.97399441001337	32.0	32.0	32.0	32.0	32.0
4	30.979584396646008	32.0	32.0	32.0	32.0	32.0
5	30.92599343784178	32.0	32.0	32.0	32.0	32.0
6	34.591566411471625	36.0	36.0	36.0	32.0	36.0
7	34.561550613683316	36.0	36.0	36.0	32.0	36.0
8	34.58050795965488	36.0	36.0	36.0	32.0	36.0
9	34.612103536274155	36.0	36.0	36.0	32.0	36.0
10	34.41244379633005	36.0	36.0	36.0	32.0	36.0
11	34.51075464819541	36.0	36.0	36.0	32.0	36.0
12	34.47028800583303	36.0	36.0	36.0	32.0	36.0
13	34.51865354235022	36.0	36.0	36.0	32.0	36.0
14	34.428606148985295	36.0	36.0	36.0	32.0	36.0
15	34.38595212054928	36.0	36.0	36.0	32.0	36.0
16	34.408798152873985	36.0	36.0	36.0	32.0	36.0
17	34.394458621946775	36.0	36.0	36.0	32.0	36.0
18	34.29359581966217	36.0	36.0	36.0	32.0	36.0
19	34.47794385709078	36.0	36.0	36.0	32.0	36.0
20	34.39154210718192	36.0	36.0	36.0	32.0	36.0
21	34.31182403694252	36.0	36.0	36.0	32.0	36.0
22	34.274881516587676	36.0	36.0	36.0	32.0	36.0
23	34.23891116782112	36.0	36.0	36.0	32.0	36.0
24	34.21837404301859	36.0	36.0	36.0	32.0	36.0
25	33.87774942277312	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	0.0
13	0.0
14	0.0
15	1.0
16	7.0
17	6.0
18	6.0
19	6.0
20	5.0
21	11.0
22	17.0
23	25.0
24	43.0
25	55.0
26	68.0
27	115.0
28	146.0
29	156.0
30	275.0
31	353.0
32	502.0
33	747.0
34	1600.0
35	4084.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.09674282936315	21.062226543509965	12.129314535731648	23.711716091395235
2	18.55632519139628	21.096123465791713	37.80532263944586	22.542228703366142
3	20.245473326042045	24.62024547332604	28.98286547575647	26.15141572487544
4	15.15372463239762	17.268197836918215	37.40430185927816	30.173775671406005
5	17.049459229554017	34.86450358488273	33.819419127475996	14.266618058087252
6	32.59205249726577	36.006805201118	17.924413659010817	13.476728642605421
7	28.342245989304814	30.165289256198346	23.249878463782206	18.242586290714634
8	26.324744773942637	34.03014098201264	21.82790471560525	17.817209528439477
9	26.592124453087017	16.006319883325233	21.24453087019932	36.15702479338843
10	18.400583373845407	26.482741857073407	32.29217306757414	22.82450170150705
11	34.3016895587699	23.860459462744622	23.82399416555245	18.013856812933028
12	24.033552151714076	24.191587648918063	30.95064429856552	20.824215900802333
13	28.698188890239457	22.474778169442082	26.35225477087638	22.474778169442082
14	23.31348000486204	20.663668408897532	26.656132247477814	29.36671933876261
15	25.699003160709943	27.716994894237786	23.802577194262096	22.781424750790176
16	26.400875167132615	27.83517685669138	24.395283821563147	21.36866415461286
17	23.598784194528875	26.84498480243161	27.756838905775076	21.799392097264437
18	23.78115501519757	26.820668693009118	27.64741641337386	21.750759878419455
19	24.96352140077821	26.909046692607003	27.10359922178988	21.023832684824903
20	24.486322188449847	26.358662613981764	26.395136778115504	22.759878419452885
21	25.750759878419455	26.370820668693007	25.386018237082062	22.492401215805472
22	23.19756838905775	26.930091185410333	27.501519756838906	22.37082066869301
23	24.917913170375776	25.623251854554297	27.423081600389153	22.035753374680773
24	24.744649805447473	25.92412451361868	26.446984435797667	22.884241245136185
25	24.40418287937743	26.884727626459142	25.656614785992215	23.054474708171206
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2.0
1	2.0
2	1.5
3	1.0
4	1.0
5	1.0
6	3.0
7	5.0
8	5.0
9	5.0
10	12.0
11	19.0
12	19.0
13	19.0
14	38.0
15	57.0
16	57.0
17	57.0
18	107.5
19	158.0
20	158.0
21	158.0
22	215.5
23	273.0
24	273.0
25	273.0
26	341.0
27	409.0
28	409.0
29	409.0
30	509.5
31	610.0
32	610.0
33	610.0
34	719.5
35	829.0
36	829.0
37	829.0
38	875.5
39	922.0
40	922.0
41	922.0
42	881.5
43	841.0
44	841.0
45	841.0
46	838.5
47	836.0
48	836.0
49	836.0
50	801.5
51	767.0
52	767.0
53	767.0
54	732.0
55	697.0
56	697.0
57	697.0
58	617.0
59	537.0
60	537.0
61	537.0
62	466.0
63	395.0
64	395.0
65	395.0
66	342.0
67	289.0
68	289.0
69	289.0
70	234.5
71	180.0
72	180.0
73	180.0
74	140.5
75	101.0
76	101.0
77	101.0
78	72.5
79	44.0
80	44.0
81	44.0
82	28.0
83	12.0
84	12.0
85	12.0
86	12.0
87	12.0
88	12.0
89	12.0
90	8.0
91	4.0
92	4.0
93	4.0
94	4.5
95	5.0
96	5.0
97	5.0
98	114.5
99	224.0
100	224.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.012152144853566653
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.012152144853566653
8	0.012152144853566653
9	0.012152144853566653
10	0.012152144853566653
11	0.024304289707133307
12	0.03645643456069996
13	0.024304289707133307
14	0.024304289707133307
15	0.03645643456069996
16	0.024304289707133307
17	0.04860857941426661
18	0.04860857941426661
19	0.06076072426783327
20	0.04860857941426661
21	0.04860857941426661
22	0.04860857941426661
23	0.07291286912139992
24	0.06076072426783327
25	0.06076072426783327
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	8229.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	92.31984445254588
#Duplication Level	Percentage of deduplicated	Percentage of total
1	95.02435171778333	87.72633369789767
2	4.593918652099513	8.482197107789524
3	0.2632618138738976	0.7291286912139993
4	0.0658154534684744	0.24304289707133309
5	0.013163090693694878	0.06076072426783327
6	0.0	0.0
7	0.013163090693694878	0.08506501397496659
8	0.0	0.0
9	0.0	0.0
>10	0.013163090693694878	0.14582573824279985
>50	0.0	0.0
>100	0.013163090693694878	2.527646129541864
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGGGGGGGGGGGGGGGGGGGGGGGG	208	2.527646129541864	No Hit
ATTCCATTCCATTCCATTCCATTCC	12	0.14582573824279985	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE