FastQCFastQC Report
Thu 2 Feb 2017
SRR4063883_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063883_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8229
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGT610.7412808360675659No Hit
ATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTT340.4131729250212663TruSeq Adapter, Index 11 (95% over 23bp)
ACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCT180.21873860736419978RNA PCR Primer, Index 11 (96% over 25bp)

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers