##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063883_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8229
Sequences flagged as poor quality	0
Sequence length	50
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.39992708713088	32.0	32.0	32.0	32.0	32.0
2	31.494470774091628	32.0	32.0	32.0	32.0	32.0
3	31.54988455462389	32.0	32.0	32.0	32.0	32.0
4	31.619516344634828	32.0	32.0	32.0	32.0	32.0
5	31.60116660590594	32.0	32.0	32.0	32.0	32.0
6	35.12273666302102	36.0	36.0	36.0	36.0	36.0
7	35.12273666302102	36.0	36.0	36.0	36.0	36.0
8	35.056507473569084	36.0	36.0	36.0	36.0	36.0
9	35.09430064406368	36.0	36.0	36.0	36.0	36.0
10	34.996597399441	36.0	36.0	36.0	36.0	36.0
11	35.21205492769474	36.0	36.0	36.0	36.0	36.0
12	35.08494349252643	36.0	36.0	36.0	36.0	36.0
13	35.081783934864504	36.0	36.0	36.0	36.0	36.0
14	35.02709928302345	36.0	36.0	36.0	36.0	36.0
15	35.13051403572731	36.0	36.0	36.0	36.0	36.0
16	35.04775792927452	36.0	36.0	36.0	36.0	36.0
17	35.02211690363349	36.0	36.0	36.0	36.0	36.0
18	35.00072912869121	36.0	36.0	36.0	36.0	36.0
19	35.0292866690971	36.0	36.0	36.0	36.0	36.0
20	35.00874954429457	36.0	36.0	36.0	36.0	36.0
21	35.07485721229797	36.0	36.0	36.0	36.0	36.0
22	35.08846761453397	36.0	36.0	36.0	36.0	36.0
23	35.045205978855265	36.0	36.0	36.0	36.0	36.0
24	34.927694738121275	36.0	36.0	36.0	32.0	36.0
25	34.928059302466885	36.0	36.0	36.0	36.0	36.0
26	34.857333819419125	36.0	36.0	36.0	32.0	36.0
27	34.80763154696804	36.0	36.0	36.0	32.0	36.0
28	34.82124194920404	36.0	36.0	36.0	32.0	36.0
29	34.752460809332845	36.0	36.0	36.0	32.0	36.0
30	34.81115566897557	36.0	36.0	36.0	32.0	36.0
31	34.707011787580505	36.0	36.0	36.0	32.0	36.0
32	34.685502491189695	36.0	36.0	36.0	32.0	36.0
33	34.690606392028194	36.0	36.0	36.0	32.0	36.0
34	34.6074857212298	36.0	36.0	36.0	32.0	36.0
35	34.55997083485235	36.0	36.0	36.0	32.0	36.0
36	34.51877506379876	36.0	36.0	36.0	32.0	36.0
37	34.334305504921616	36.0	36.0	36.0	32.0	36.0
38	34.2865475756471	36.0	36.0	36.0	32.0	36.0
39	34.21655122129056	36.0	36.0	36.0	32.0	36.0
40	34.240977032446224	36.0	36.0	36.0	32.0	36.0
41	34.273544780653786	36.0	36.0	36.0	32.0	36.0
42	34.38218495564467	36.0	36.0	36.0	32.0	36.0
43	34.310001215214484	36.0	36.0	36.0	32.0	36.0
44	34.20561429092235	36.0	36.0	36.0	32.0	36.0
45	34.33406246202455	36.0	36.0	36.0	32.0	36.0
46	34.23429335277677	36.0	36.0	36.0	32.0	36.0
47	34.102321059667034	36.0	36.0	36.0	32.0	36.0
48	34.22408555109977	36.0	36.0	36.0	32.0	36.0
49	34.09903998055657	36.0	36.0	36.0	32.0	36.0
50	33.610402235994655	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	0.0
20	0.0
21	2.0
22	4.0
23	5.0
24	11.0
25	32.0
26	40.0
27	44.0
28	105.0
29	157.0
30	238.0
31	304.0
32	433.0
33	593.0
34	1173.0
35	5087.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.862559241706165	22.51792441365901	12.01847126017742	26.601045084457407
2	14.679790983108518	22.274881516587676	39.372949325555965	23.672378174747845
3	16.867177056750513	27.014218009478675	30.319601409648804	25.799003524122003
4	12.735447806537852	19.054563130392516	37.55012759752096	30.659861465548673
5	14.862073155912018	36.699477457771295	34.67006926722567	13.76838011909102
6	30.356057844209506	38.352169157856366	17.644914327378782	13.646858670555353
7	26.443067201361043	31.073034390569937	23.550856726212178	18.933041681856846
8	23.380726698262244	35.11969862680763	22.894640904119576	18.604933770810547
9	23.31996597399441	16.02867906185442	21.75233928788431	38.89901567626686
10	15.518288978004618	27.269413051403575	33.503463361283266	23.708834609308543
11	32.38546603475513	23.46579171223721	25.20354842629724	18.945193826710412
12	21.691578563616478	25.373678454247173	30.027949933163207	22.906793048973142
13	26.831935836675175	21.72803499817718	27.68258597642484	23.75744318872281
14	21.484992101105846	22.40855510997691	26.139263580021876	29.967189208895366
15	23.441487422530077	27.986389597764006	23.587313160772876	24.98480981893304
16	23.83035605784421	28.788431158099403	23.86681249240491	23.514400291651476
17	20.622189816502612	28.76412686839227	28.010693887471138	22.602989427633975
18	22.177664357759145	27.64612954186414	27.767650990399805	22.40855510997691
19	21.80094786729858	27.269413051403575	27.00206586462511	23.927573216672744
20	23.04046664236238	27.64612954186414	25.56811277190424	23.745291043869244
21	24.72961477700814	26.977761574917974	26.175720014582577	22.11690363349131
22	21.861708591566412	29.09223477943857	26.613197229310977	22.432859399684045
23	23.06477093206951	26.783327257260908	27.050674444039373	23.101227366630212
24	24.073398954915543	26.904848705796574	27.07497873374651	21.946773605541377
25	24.510876169643943	27.378782355085672	25.507352047636406	22.602989427633975
26	24.717462632154575	27.585368817596308	25.908372827804104	21.788795722445013
27	22.62729371734111	28.533236116174503	26.20002430428971	22.639445862194677
28	22.359946530562645	27.70689026613197	26.175720014582577	23.75744318872281
29	23.635921740187143	27.014218009478675	27.07497873374651	22.274881516587676
30	23.016162352655243	27.123587313160773	26.33369789767894	23.526552436505042
31	23.47794385709078	27.17219589257504	26.58889293960384	22.760967310730344
32	22.736663021023208	27.93778101834974	27.25726090655001	22.068295054077044
33	21.788795722445013	26.929152995503706	27.63397739701057	23.64807388504071
34	22.44501154453761	27.220804471989307	28.278041074249604	22.056142909223478
35	22.056142909223478	26.77117511240734	28.168671770567506	23.004010207801677
36	23.24705310487301	27.403086644792808	25.859764248389837	23.490096001944345
37	22.62729371734111	27.561064527889172	27.342325920524974	22.469315834244743
38	22.177664357759145	27.46384736906064	27.57321667274274	22.785271600437476
39	23.016162352655243	26.49167578077531	27.123587313160773	23.368574553408678
40	22.214120792319843	26.114959290314744	28.98286547575647	22.688054441608944
41	24.19492040345121	26.443067201361043	26.467371491068175	22.894640904119576
42	23.13768380119091	25.531656337343538	28.72767043383157	22.602989427633975
43	22.11690363349131	24.838984080690242	29.541864139020536	23.50224814679791
44	22.359946530562645	25.884068538096972	28.29019321910317	23.46579171223721
45	21.703730708470044	24.996961963786607	29.748450601531168	23.550856726212178
46	24.207072548304776	25.033418398347308	27.24510876169644	23.514400291651476
47	21.776643577591447	25.38583059910074	29.432494835338435	23.405030987969376
48	21.7671366067088	25.559066601847352	28.52455031599417	24.149246475449683
49	21.39992708713088	25.045570543200874	29.724146311824036	23.83035605784421
50	21.059667031231015	25.227852716004374	29.979341353748936	23.733138899015678
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1.0
1	0.5
2	0.0
3	0.0
4	0.0
5	0.5
6	1.0
7	0.5
8	0.0
9	0.5
10	1.0
11	2.0
12	3.0
13	5.0
14	7.0
15	5.5
16	4.0
17	10.0
18	16.0
19	20.5
20	25.0
21	41.0
22	57.0
23	68.0
24	79.0
25	105.0
26	131.0
27	159.5
28	188.0
29	199.5
30	211.0
31	269.0
32	327.0
33	370.0
34	413.0
35	465.5
36	518.0
37	541.0
38	564.0
39	551.5
40	539.0
41	537.5
42	536.0
43	526.0
44	516.0
45	498.0
46	480.0
47	443.0
48	406.0
49	415.0
50	424.0
51	398.5
52	373.0
53	438.0
54	503.0
55	433.5
56	364.0
57	340.5
58	317.0
59	308.0
60	299.0
61	275.5
62	252.0
63	219.5
64	187.0
65	167.0
66	147.0
67	126.5
68	106.0
69	91.0
70	76.0
71	69.0
72	62.0
73	43.0
74	24.0
75	25.5
76	27.0
77	20.5
78	14.0
79	15.0
80	16.0
81	12.0
82	8.0
83	5.0
84	2.0
85	1.5
86	1.0
87	1.0
88	1.0
89	1.0
90	1.0
91	0.5
92	0.0
93	0.5
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.012152144853566653
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	8229.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	93.64442824158465
#Duplication Level	Percentage of deduplicated	Percentage of total
1	95.17259278484298	89.12383035605784
2	4.4121463794445885	8.263458500425324
3	0.25953802232026996	0.7291286912139993
4	0.06488450558006749	0.24304289707133309
5	0.02595380223202699	0.12152144853566654
6	0.02595380223202699	0.14582573824279985
7	0.0	0.0
8	0.0	0.0
9	0.0	0.0
>10	0.02595380223202699	0.631911532385466
>50	0.012976901116013495	0.7412808360675659
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGT	61	0.7412808360675659	No Hit
ATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTT	34	0.4131729250212663	TruSeq Adapter, Index 11 (95% over 23bp)
ACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCT	18	0.21873860736419978	RNA PCR Primer, Index 11 (96% over 25bp)
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	0.02430428970713331	0.0
26	0.0	0.0	0.0	0.02430428970713331	0.0
27	0.0	0.0	0.0	0.02430428970713331	0.0
28	0.0	0.0	0.0	0.03645643456069996	0.0
29	0.0	0.0	0.0	0.14582573824279985	0.0
30	0.0	0.0	0.0	0.21873860736419978	0.0
31	0.0	0.0	0.0	0.3038036213391664	0.0
32	0.0	0.0	0.0	0.38886863531413296	0.0
33	0.0	0.0	0.0	0.4982379389962328	0.0
34	0.0	0.0	0.0	0.595455097824766	0.0
35	0.0	0.0	0.0	0.8263458500425325	0.0
36	0.0	0.0	0.0	1.0815408919674323	0.0
37	0.0	0.0	0.0	1.4582573824279985	0.0
38	0.0	0.0	0.0	1.9443431765706647	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE