FastQCFastQC Report
Thu 2 Feb 2017
SRR4063882_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063882_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences171673
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT49832.9026113599692436No Hit
TATCAACGCAGAGTACTTTTTTTTT39392.294478456134628No Hit
GTACTTTTTTTTTTTTTTTTTTTTT37302.1727353748114147No Hit
GTACATGGGGTGGTATCAACGCAAA29661.7277032497830178No Hit
GAGTACTTTTTTTTTTTTTTTTTTT23441.3653865197206316No Hit
ACGCAGAGTACTTTTTTTTTTTTTT19851.156268021179801No Hit
GGTATCAACGCAGAGTACTTTTTTT18171.05840755389607No Hit
GTATCAACGCAGAGTACATGGGGTG17241.0042347952211472No Hit
GTACATGGGTGGTATCAACGCAAAA13270.7729811909851869No Hit
TATCAACGCAGAGTACATGGGGTGG12660.7374485213166893No Hit
GCAGAGTACTTTTTTTTTTTTTTTT10050.5854152953580353No Hit
GTACATGGGAGTGGTATCAACGCAA10030.5842502897951338No Hit
GGTATCAACGCAGAGTACATGGGGT8440.49163234754445956No Hit
ACGCAGAGTACATGGGGTGGTATCA6780.3949368858236298No Hit
ATCAACGCAGAGTACTTTTTTTTTT5970.3477541605261165No Hit
GTATCAACGCAGAGTACATGGGAGT5910.34425914383741185No Hit
GTACATGGAAGCAGTGGTATCAACG5570.32445404926808524No Hit
GTATCAACGCAGAGTACATGGGAAG5530.32212403814228213No Hit
GTATCAACGCAGAGTACATGGGTGG5510.3209590325793806No Hit
TCCATGTACTCTGCGTTGATACCAC4900.2854263629108829No Hit
GAGTACATGGGGTGGTATCAACGCA4400.25630122383834386No Hit
TATCAACGCAGAGTACATGGGTGGT4170.24290365986497586No Hit
TATCAACGCAGAGTACATGGGAGTG3850.22426357085855086No Hit
ACTTTTTTTTTTTTTTTTTTTTTTT3650.2126135152295352No Hit
GTATCAACGCAGAGTACATGGGCAG3510.20445847628922428No Hit
GTACATGGGAAGCAGTGGTATCAAC3460.20154596238197037No Hit
ATCAACGCAGAGTACATGGGGTGGT3240.18873090119005317No Hit
CCCATGTACTCTGCGTTGATACCAC3160.18407087893844695No Hit
TATCAACGCAGAGTACATGGGAAGC3100.18057586224974226No Hit
GTACATGGGCAGTGGTATCAACGCA2980.17358582887233287No Hit
GTACATGGGGTATCAACGCAAAAAA2960.17242082330943131No Hit
CTTTTTTTTTTTTTTTTTTTTTTTT2920.17009081218362818No Hit
GGTATCAACGCAGAGTACATGGGAG2860.16659579549492348No Hit
CAACGCAGAGTACTTTTTTTTTTTT2840.16543078993202193No Hit
GGTATCAACGCAGAGTACATGGGTG2820.16426578436912034No Hit
GTATCAACGCAGAGTACATGGGGTA2730.15902325933606332No Hit
GGTATCAACGCAGAGTACATGGGAA2640.1537807343030063No Hit
CATGTACTCTGCGTTGATACCACTG2560.14912071205140004No Hit
GGTGGTATCAACGCAAAAAAAAAAA2550.14853820926994926No Hit
ACGCAGAGTACATGGGAGTGGTATC2390.13921816476673676No Hit
TCAACGCAGAGTACTTTTTTTTTTT2380.13863566198528599No Hit
ACGCAGAGTACATGGGTGGTATCAA2180.12698560635627035No Hit
GTATCAACGCAGAGTACATGGGGCA2070.12057807576031176No Hit
AACGCAGAGTACTTTTTTTTTTTTT2020.11766556185305785No Hit
GAGTACATGGAAGCAGTGGTATCAA1970.11475304794580393No Hit
GGGTGGTATCAACGCAAAAAAAAAA1940.1130055396014516No Hit
GTATCAACGCAGAGTACATGGGGGT1770.10310299231678832No Hit
TATCAACGCAGAGTACATGGGGTAT1720.1001904784095344No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGAAA307.675806E-419.00320819
ATTACGC400.00525810314.2482513
GGATTGC550.00305216912.0894258
TGGGTGG4000.010.6861896
ATGGGTG4600.010.1183235
ACATGGA1553.952664E-89.8052493
GAGTGGT2002.5283953E-109.4988359
CATGGGT5400.09.322934
TCCATGT1701.6177364E-78.9479021
TCTGCGT3100.08.57959310
CCATTAC1000.00285788448.556431
ATGGAAG1451.659391E-58.5161965
GGGGTGG6550.08.4111817
TGCGTTG3050.08.40880512
CTGCGTT2951.8189894E-128.37185411
TACTCTG3200.08.311487
CTCTGCG3200.08.311489
TGGGGTA1401.0236597E-48.1418586
GGAGTGG2355.293259E-98.0841148
CATGGAA1659.547115E-68.0596174
Produced by FastQC (version 0.11.5)