##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063882_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	171673
Sequences flagged as poor quality	0
Sequence length	25
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.653760346705656	32.0	32.0	32.0	32.0	32.0
2	30.578640788009764	32.0	32.0	32.0	32.0	32.0
3	30.70892918513686	32.0	32.0	32.0	32.0	32.0
4	30.56477139678342	32.0	32.0	32.0	32.0	32.0
5	30.6392443773919	32.0	32.0	32.0	32.0	32.0
6	34.104413623575056	36.0	36.0	36.0	32.0	36.0
7	33.850238534889	36.0	36.0	36.0	32.0	36.0
8	33.6694762717492	36.0	36.0	36.0	27.0	36.0
9	33.70594677089583	36.0	36.0	36.0	32.0	36.0
10	33.64788289364082	36.0	36.0	36.0	27.0	36.0
11	34.061698694611266	36.0	36.0	36.0	32.0	36.0
12	33.88958077274819	36.0	36.0	36.0	32.0	36.0
13	33.91413909001415	36.0	36.0	36.0	32.0	36.0
14	33.869612577400055	36.0	36.0	36.0	32.0	36.0
15	33.88752453792967	36.0	36.0	36.0	32.0	36.0
16	33.83264112586138	36.0	36.0	36.0	32.0	36.0
17	33.71330960605337	36.0	36.0	36.0	27.0	36.0
18	33.70472351505479	36.0	36.0	36.0	27.0	36.0
19	33.659317423240694	36.0	36.0	36.0	27.0	36.0
20	33.456798681213705	36.0	36.0	36.0	21.0	36.0
21	33.410711061145314	36.0	36.0	36.0	21.0	36.0
22	33.49354878169543	36.0	36.0	36.0	21.0	36.0
23	33.571714829938315	36.0	36.0	36.0	27.0	36.0
24	33.61331135356171	36.0	36.0	36.0	27.0	36.0
25	33.274300559785175	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	1.0
5	2.0
6	9.0
7	5.0
8	11.0
9	11.0
10	10.0
11	5.0
12	18.0
13	15.0
14	77.0
15	105.0
16	123.0
17	162.0
18	231.0
19	291.0
20	424.0
21	679.0
22	885.0
23	1156.0
24	1529.0
25	2047.0
26	2700.0
27	3303.0
28	4336.0
29	5575.0
30	6855.0
31	8996.0
32	12289.0
33	16752.0
34	33668.0
35	69403.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	52.04780941502815	16.690171214116713	12.900500005827572	18.361519365027565
2	15.02132171319383	21.997483338770564	41.30936291187025	21.671832036165352
3	19.10409506611522	33.67507427040252	27.237141026387835	19.983689637094425
4	10.869691850643678	15.223976233471195	34.09040601153376	39.815925904351374
5	11.747400308758847	42.04945967201654	28.448923713262065	17.75421630596254
6	27.41821783331877	36.38090244399778	20.566285065105305	15.634594657578141
7	27.100691005278676	33.86450237132503	20.46447673537877	18.570329888017525
8	32.60062587047861	27.09366495533243	19.098373552601675	21.207335621587287
9	32.37671715905978	11.82501151086684	20.01200627123682	35.786265058836555
10	19.195033807414315	27.341454884588483	27.65854511541152	25.80496619258568
11	33.58687859553411	24.578736004010096	22.016471699102976	19.817913701352825
12	27.94536837871395	26.777771948539485	28.20535240660103	17.071507266145534
13	32.04973506111956	22.126622714209933	27.28025228943334	18.543389935237162
14	25.98086933205873	18.881771073171016	31.15407707059461	23.98328252417564
15	24.32558464874443	26.06379258084824	30.325957704772787	19.284665065634545
16	20.60995727267958	28.039731161798393	28.160393580992466	23.18991798452956
17	18.197185725777306	26.334565209788174	29.144759084603102	26.32348997983142
18	19.072826061609497	26.40906652986032	33.654361868339436	20.863745540190752
19	21.990334559487927	24.297098618972946	34.30473536630154	19.407831455237584
20	26.39584438601503	20.59734036040973	32.688148221562784	20.318667032012453
21	28.403784606236552	20.86488629275317	30.81842401347814	19.91290508753214
22	24.92304642865471	22.554975164983794	31.569036681202338	20.952941725159153
23	22.024312742325744	25.210039938197827	32.66711366352797	20.098533655948458
24	21.416536952504213	25.738787740991427	32.66639849356676	20.178276812937604
25	22.17415890819842	26.11101070930958	32.11277130348096	19.602059079011035
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	296.0
1	296.0
2	599.5
3	903.0
4	903.0
5	903.0
6	2514.0
7	4125.0
8	4125.0
9	4125.0
10	3508.5
11	2892.0
12	2892.0
13	2892.0
14	1786.0
15	680.0
16	680.0
17	680.0
18	1313.5
19	1947.0
20	1947.0
21	1947.0
22	3019.5
23	4092.0
24	4092.0
25	4092.0
26	6131.5
27	8171.0
28	8171.0
29	8171.0
30	9216.0
31	10261.0
32	10261.0
33	10261.0
34	9797.0
35	9333.0
36	9333.0
37	9333.0
38	10130.0
39	10927.0
40	10927.0
41	10927.0
42	13291.5
43	15656.0
44	15656.0
45	15656.0
46	21570.0
47	27484.0
48	27484.0
49	27484.0
50	27220.0
51	26956.0
52	26956.0
53	26956.0
54	20973.0
55	14990.0
56	14990.0
57	14990.0
58	13453.0
59	11916.0
60	11916.0
61	11916.0
62	10064.5
63	8213.0
64	8213.0
65	8213.0
66	7067.0
67	5921.0
68	5921.0
69	5921.0
70	4620.0
71	3319.0
72	3319.0
73	3319.0
74	2652.0
75	1985.0
76	1985.0
77	1985.0
78	1449.0
79	913.0
80	913.0
81	913.0
82	653.5
83	394.0
84	394.0
85	394.0
86	260.5
87	127.0
88	127.0
89	127.0
90	80.0
91	33.0
92	33.0
93	33.0
94	27.0
95	21.0
96	21.0
97	21.0
98	69.5
99	118.0
100	118.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.043687708608808606
2	0.009902547284663284
3	0.0017475083443523444
4	0.0017475083443523444
5	0.010485050066114065
6	0.01631007788062188
7	0.022717608476580473
8	0.04194020026445626
9	0.05592026701927502
10	0.0658228143039383
11	0.06174529483378283
12	0.0722303448998969
13	0.0722303448998969
14	0.06757032264829065
15	0.06815282542974142
16	0.06990033377409377
17	0.06931783099264299
18	0.08213289218456017
19	0.07805537271440471
20	0.08504540609181409
21	0.07922037827730627
22	0.08213289218456017
23	0.09203543946922348
24	0.08271539496601096
25	0.08155038940310941
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	171673.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.467318681446706
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.89878554771495	26.118842217471588
2	15.851479451225053	12.19527823245356
3	6.579545110390987	7.5929237562109355
4	3.345043762682092	5.146994576899105
5	1.8322783851721738	3.5241418277772274
6	1.0690814379599625	2.4674817822255104
7	0.745025591326206	2.0061395793164913
8	0.511826524122475	1.575087521042913
9	0.3921984312062751	1.3578139835617715
>10	1.5869650807111058	10.27127154532163
>50	0.07117114388685303	1.9408992677940038
>100	0.08782822011569097	6.8950854240329
>500	0.0105999576001696	2.546119657721366
>1k	0.018171355886005026	16.361920628171
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	4983	2.9026113599692436	No Hit
TATCAACGCAGAGTACTTTTTTTTT	3939	2.294478456134628	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	3730	2.1727353748114147	No Hit
GTACATGGGGTGGTATCAACGCAAA	2966	1.7277032497830178	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	2344	1.3653865197206316	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1985	1.156268021179801	No Hit
GGTATCAACGCAGAGTACTTTTTTT	1817	1.05840755389607	No Hit
GTATCAACGCAGAGTACATGGGGTG	1724	1.0042347952211472	No Hit
GTACATGGGTGGTATCAACGCAAAA	1327	0.7729811909851869	No Hit
TATCAACGCAGAGTACATGGGGTGG	1266	0.7374485213166893	No Hit
GCAGAGTACTTTTTTTTTTTTTTTT	1005	0.5854152953580353	No Hit
GTACATGGGAGTGGTATCAACGCAA	1003	0.5842502897951338	No Hit
GGTATCAACGCAGAGTACATGGGGT	844	0.49163234754445956	No Hit
ACGCAGAGTACATGGGGTGGTATCA	678	0.3949368858236298	No Hit
ATCAACGCAGAGTACTTTTTTTTTT	597	0.3477541605261165	No Hit
GTATCAACGCAGAGTACATGGGAGT	591	0.34425914383741185	No Hit
GTACATGGAAGCAGTGGTATCAACG	557	0.32445404926808524	No Hit
GTATCAACGCAGAGTACATGGGAAG	553	0.32212403814228213	No Hit
GTATCAACGCAGAGTACATGGGTGG	551	0.3209590325793806	No Hit
TCCATGTACTCTGCGTTGATACCAC	490	0.2854263629108829	No Hit
GAGTACATGGGGTGGTATCAACGCA	440	0.25630122383834386	No Hit
TATCAACGCAGAGTACATGGGTGGT	417	0.24290365986497586	No Hit
TATCAACGCAGAGTACATGGGAGTG	385	0.22426357085855086	No Hit
ACTTTTTTTTTTTTTTTTTTTTTTT	365	0.2126135152295352	No Hit
GTATCAACGCAGAGTACATGGGCAG	351	0.20445847628922428	No Hit
GTACATGGGAAGCAGTGGTATCAAC	346	0.20154596238197037	No Hit
ATCAACGCAGAGTACATGGGGTGGT	324	0.18873090119005317	No Hit
CCCATGTACTCTGCGTTGATACCAC	316	0.18407087893844695	No Hit
TATCAACGCAGAGTACATGGGAAGC	310	0.18057586224974226	No Hit
GTACATGGGCAGTGGTATCAACGCA	298	0.17358582887233287	No Hit
GTACATGGGGTATCAACGCAAAAAA	296	0.17242082330943131	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTT	292	0.17009081218362818	No Hit
GGTATCAACGCAGAGTACATGGGAG	286	0.16659579549492348	No Hit
CAACGCAGAGTACTTTTTTTTTTTT	284	0.16543078993202193	No Hit
GGTATCAACGCAGAGTACATGGGTG	282	0.16426578436912034	No Hit
GTATCAACGCAGAGTACATGGGGTA	273	0.15902325933606332	No Hit
GGTATCAACGCAGAGTACATGGGAA	264	0.1537807343030063	No Hit
CATGTACTCTGCGTTGATACCACTG	256	0.14912071205140004	No Hit
GGTGGTATCAACGCAAAAAAAAAAA	255	0.14853820926994926	No Hit
ACGCAGAGTACATGGGAGTGGTATC	239	0.13921816476673676	No Hit
TCAACGCAGAGTACTTTTTTTTTTT	238	0.13863566198528599	No Hit
ACGCAGAGTACATGGGTGGTATCAA	218	0.12698560635627035	No Hit
GTATCAACGCAGAGTACATGGGGCA	207	0.12057807576031176	No Hit
AACGCAGAGTACTTTTTTTTTTTTT	202	0.11766556185305785	No Hit
GAGTACATGGAAGCAGTGGTATCAA	197	0.11475304794580393	No Hit
GGGTGGTATCAACGCAAAAAAAAAA	194	0.1130055396014516	No Hit
GTATCAACGCAGAGTACATGGGGGT	177	0.10310299231678832	No Hit
TATCAACGCAGAGTACATGGGGTAT	172	0.1001904784095344	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGGGAAA	30	7.675806E-4	19.003208	19
ATTACGC	40	0.005258103	14.248251	3
GGATTGC	55	0.003052169	12.089425	8
TGGGTGG	400	0.0	10.686189	6
ATGGGTG	460	0.0	10.118323	5
ACATGGA	155	3.952664E-8	9.805249	3
GAGTGGT	200	2.5283953E-10	9.498835	9
CATGGGT	540	0.0	9.32293	4
TCCATGT	170	1.6177364E-7	8.947902	1
TCTGCGT	310	0.0	8.579593	10
CCATTAC	100	0.0028578844	8.55643	1
ATGGAAG	145	1.659391E-5	8.516196	5
GGGGTGG	655	0.0	8.411181	7
TGCGTTG	305	0.0	8.408805	12
CTGCGTT	295	1.8189894E-12	8.371854	11
TACTCTG	320	0.0	8.31148	7
CTCTGCG	320	0.0	8.31148	9
TGGGGTA	140	1.0236597E-4	8.141858	6
GGAGTGG	235	5.293259E-9	8.084114	8
CATGGAA	165	9.547115E-6	8.059617	4
>>END_MODULE