FastQCFastQC Report
Thu 2 Feb 2017
SRR4063881_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063881_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2620
Sequences flagged as poor quality0
Sequence length25
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGG963.6641221374045805No Hit
ATCTAAGAGCTTTCGCCTGATGGTC491.870229007633588No Hit
GTACATGGGAGATGAGAGAAAGTTA311.183206106870229No Hit
CTATATACATGGCTTAATTACTCTT240.9160305343511451No Hit
CTCTTAGATCGCGCCTTTGCTCTCT230.8778625954198473No Hit
GTGTTGGCCCGCTCAATGCGTTCCA210.8015267175572519No Hit
GCATATAGCTCTCCACAGCAACTCT210.8015267175572519No Hit
ATCTTCGTAGAGAGCAAAGGCGCGA200.7633587786259541No Hit
GCTATATGCAGATTGGAAGGGAGCT170.648854961832061No Hit
ATGTCAACATCGCGCGAGATCTTGC140.5343511450381679No Hit
TGTCAACATCGCGCGAGATCTTGCT130.4961832061068702No Hit
CTACGAAGATAGCGTGGGCGCCATT120.45801526717557256No Hit
ACGTACCACCTGTGTGGTTGAAGGA110.41984732824427484No Hit
GATCAGGAGTCCTTCATCCGCGGAC110.41984732824427484No Hit
TCCTTCATCCGCGGACTGGTGAAGT100.38167938931297707No Hit
GCAATATGTCAACATCGCGCGAGAT100.38167938931297707No Hit
ATGTTGACATATTGCAATGCGATCC90.3435114503816794No Hit
ACGTAGCACCTGCCGAGTCTCGCAT90.3435114503816794No Hit
ATTCAGAAGAGTCTTCGTGGCCTGG90.3435114503816794No Hit
CCTTTTACATTTCCCTTCCTGAGCT90.3435114503816794No Hit
GGTGAAGTCCGACTTGGCCGAAGAC90.3435114503816794No Hit
CTCCTGATCAAGTCCGACTTCCTTC90.3435114503816794No Hit
GTGCTACGTACCACCTGTGTGGTTG90.3435114503816794No Hit
TATCAACGCAGAGTACATGGGAGAT90.3435114503816794No Hit
CGTACCACCTGTGTGGTTGAAGGAA90.3435114503816794No Hit
GGTGGTACGTAGCACCTGCCGAGTC90.3435114503816794No Hit
TCCTTCAACCACACAGGTGGTACGT80.3053435114503817No Hit
GCTCTCCACAGCAACTCTCATTCCA80.3053435114503817No Hit
GTTCCAGGCCACGAAGACTCTTCTG80.3053435114503817No Hit
GATTGGAAGGGAGCTCAGGAAGGGA80.3053435114503817No Hit
GATTAAGACTATACACACTATATAC80.3053435114503817No Hit
AAGATAGCGTGGGCGCCATTGAGCG80.3053435114503817No Hit
GACTGGTGAAGTCCGACTTGGCCGA70.26717557251908397No Hit
GTCCTTCATCCGCGGACTGGTGAAG70.26717557251908397No Hit
GTTGAAGGAAGTCGGACTTGATCAG70.26717557251908397No Hit
GTATCAACGCAGAGTACATGGGAGA70.26717557251908397No Hit
CACGCTATCTTCGTAGAGAGCAAAG70.26717557251908397No Hit
GCATTGCAATATGTCAACATCGCGC70.26717557251908397No Hit
CCATTGAGCGACTTCCCAGCGAAGC60.22900763358778628No Hit
CTTCTGAATGCAGGAACGCTTGCCT60.22900763358778628No Hit
CTCCACAGCAACTCTCATTCCACCT60.22900763358778628No Hit
GCTGTGGAGAGCTATATGCAGATTG60.22900763358778628No Hit
GAGCTCAGGAAGGGAAATGTAAAAG60.22900763358778628No Hit
CTATTAGGGTTGTGAGTGTCCTGGG60.22900763358778628No Hit
TCTGAATGCAGGAACGCTTGCCTTC60.22900763358778628No Hit
GATAGCGTGGGCGCCATTGAGCGAC60.22900763358778628No Hit
GTACCGGACTGGGCCGGTACCTCCC60.22900763358778628No Hit
AGTTAATGGAGGCAGGAGGCAAGAT60.22900763358778628No Hit
CACTATATACATGGCTTAATTACTC60.22900763358778628No Hit
GCTCTCTACGAAGATAGCGTGGGCG60.22900763358778628No Hit
CTCTCATTCCACCTCGTGCTTCGCT60.22900763358778628No Hit
ACGCAGAGTACATGGGAGATGAGAG60.22900763358778628No Hit
GTCCGCGGATGAAGGACTCCTGATC60.22900763358778628No Hit
TATCAACGCAGAGTACTTTTTTTTT60.22900763358778628No Hit
ACTATACACACTATATACATGGCTT60.22900763358778628No Hit
CTTCCAATCTGCATATAGCTCTCCA60.22900763358778628No Hit
GATGAAGGACTCCTGATCAAGTCCG60.22900763358778628No Hit
ATATACATGGCTTAATTACTCTTGA60.22900763358778628No Hit
TCCCTGACCTGTGCATACCACATCC60.22900763358778628No Hit
GTGGAGAGCTATATGCAGATTGGAA50.19083969465648853No Hit
CGCTATCTTCGTAGAGAGCAAAGGC50.19083969465648853No Hit
CTATACACACTATATACATGGCTTA50.19083969465648853No Hit
GATCGAGACCATCAGGCGAAAGCTC50.19083969465648853No Hit
GTACGTAGCACCTGCCGAGTCTCGC50.19083969465648853No Hit
ATCTTGCTGTCGATGCGAGACTCGG50.19083969465648853No Hit
CCTTTGCTCTCTACGAAGATAGCGT50.19083969465648853No Hit
GCTCAATGCGTTCCAGGCCACGAAG50.19083969465648853No Hit
CATTGCAATATGTCAACATCGCGCG50.19083969465648853No Hit
CAGAAGAGTCTTCGTGGCCTGGAAC50.19083969465648853No Hit
GGGCCGGGACTGCTGCAAGCGGTTC50.19083969465648853No Hit
TGTAAAAGGAGGAGAGAAGGCAAGC50.19083969465648853No Hit
TTGGAAGGGAGCTCAGGAAGGGAAA50.19083969465648853No Hit
GTGGAGCCGGGCGGGAACCTGTCAG50.19083969465648853No Hit
TTCCTGCATTCAGAAGAGTCTTCGT40.15267175572519084No Hit
TAGCTCTCCACAGCAACTCTCATTC40.15267175572519084No Hit
GAGTTGCTGTGGAGAGCTATATGCA40.15267175572519084No Hit
TCCGCGGACTGGTGAAGTCCGACTT40.15267175572519084No Hit
GTATCAACGCAGAGTACATGGGGGA40.15267175572519084No Hit
TCGCTGGGAAGTCGCTCAATGGCGC40.15267175572519084No Hit
GCTACGTACCACCTGTGTGGTTGAA40.15267175572519084No Hit
GGAGTCCTTCATCCGCGGACTGGTG40.15267175572519084No Hit
GAGCAAAGGCGCGATCTAAGAGCTT40.15267175572519084No Hit
GGCAAGATGGGGATCGCATTGCAAT40.15267175572519084No Hit
TCCCAGCGAAGCACGAGGTGGAATG40.15267175572519084No Hit
ATACATGGCTTAATTACTCTTGAGA40.15267175572519084No Hit
CTGGGAAGTCGCTCAATGGCGCCCA40.15267175572519084No Hit
CTTCCATGCTCGCCGCAGGTTCTCT40.15267175572519084No Hit
GATCTTGTCTTCGGCCAAGTCGGAC40.15267175572519084No Hit
GGACTTGGCACCTCATTGTTCATGG40.15267175572519084No Hit
ATACTATACGGCGACCGCTTCACCC40.15267175572519084No Hit
GGCGCGATCTAAGAGCTTTCGCCTG40.15267175572519084No Hit
GGCGGGAACCTGTCAGCGACGAAGC40.15267175572519084No Hit
GGTTTAGGGAGAGTGGACAGGACTT40.15267175572519084No Hit
TCACCAGTCCGCGGATGAAGGACTC40.15267175572519084No Hit
GGCCAACACCTCGGAAAGATCTCAA40.15267175572519084No Hit
TTGCAATATGTCAACATCGCGCGAG40.15267175572519084No Hit
TTCTGAATCTGTCCTCGTGTCGTTC40.15267175572519084No Hit
GGCTGGCCTTGCGCAACATCACCGC40.15267175572519084No Hit
CCACCTGTGTGGTTGAAGGAAGTCG40.15267175572519084No Hit
CCTTCAACCACACAGGTGGTACGTA40.15267175572519084No Hit
AGATCTTGCTGTCGATGCGAGACTC40.15267175572519084No Hit
GGTTGAAGGAAGTCGGACTTGATCA40.15267175572519084No Hit
CTGGTGAAGTCCGACTTGGCCGAAG40.15267175572519084No Hit
CAACCACACAGGTGGTACGTAGCAC40.15267175572519084No Hit
GGGGAGGTACCGGCCCAGTCCGGTA40.15267175572519084No Hit
GAATGAGAGTTGCTGTGGAGAGCTA40.15267175572519084No Hit
ACTATACGGCGACCGCTTCACCCTC40.15267175572519084No Hit
GGGTAATCAGAGGCCAGTCTCTGTC40.15267175572519084No Hit
CTTGAGATCTTTCCGAGGTGTTGGC40.15267175572519084No Hit
GCCCAGTCCGGTACGACGGGTAACG40.15267175572519084No Hit
CGGCAGGTGCTACGTACCACCTGTG30.11450381679389314No Hit
ACCTCGTGCTTCGCTGGGAAGTCGC30.11450381679389314No Hit
GTCTCGATCTTGTCTTCGGCCAAGT30.11450381679389314No Hit
ATCTGGTGACGGCGTGGCCATTGAT30.11450381679389314No Hit
CCACAGCAACTCTCATTCCACCTCG30.11450381679389314No Hit
GACTATACACACTATATACATGGCT30.11450381679389314No Hit
AGGCAAGATGGGGATCGCATTGCAA30.11450381679389314No Hit
TCGTTACCCGTCGTACCGGACTGGG30.11450381679389314No Hit
GTATCAACGCAGAGTACATGGGGCA30.11450381679389314No Hit
GCACTAATGGAAGCGGGCTCAGGAC30.11450381679389314No Hit
GAGTGGAAATCGGCGATGAACCAGT30.11450381679389314No Hit
GTACATGGGGCACTTCCCAAGCGAC30.11450381679389314No Hit
GGTCAATGTCCAGCCCATCCACGCC30.11450381679389314No Hit
AAGACTCTTCTGAATGCAGGAACGC30.11450381679389314No Hit
CTCTACGAAGATAGCGTGGGCGCCA30.11450381679389314No Hit
TTACTCTTGAGATCTTTCCGAGGTG30.11450381679389314No Hit
TTCCAGGCCACGAAGACTCTTCTGA30.11450381679389314No Hit
GAGCTATATGCAGATTGGAAGGGAG30.11450381679389314No Hit
CAGCAACTCTCATTCCACCTCGTGC30.11450381679389314No Hit
ATCGAGACCATCAGGCGAAAGCTCT30.11450381679389314No Hit
AGACTATACACACTATATACATGGC30.11450381679389314No Hit
AAACAGGACATCCCCTCGGAATGCC30.11450381679389314No Hit
GAATGCAGGAACGCTTGCCTTCTCT30.11450381679389314No Hit
AGGTGGAATGAGAGTTGCTGTGGAG30.11450381679389314No Hit
CTCATTCCACCTCGTGCTTCGCTGG30.11450381679389314No Hit
AAGGACTCCTGATCAAGTCCGACTT30.11450381679389314No Hit
GCTCAGGACAGGTAAGTTTTGATAG30.11450381679389314No Hit
GTATAGTATCGCGAACTGGTTCATC30.11450381679389314No Hit
TGCCTTCTCTCCTCCTTTTACATTT30.11450381679389314No Hit
AGACAAGATCGAGACCATCAGGCGA30.11450381679389314No Hit
GTCCGGTACGACGGGTAACGATCCC30.11450381679389314No Hit
CCCTAAACCTCTCTTGAGTCTGCAT30.11450381679389314No Hit
GACAAGATCGAGACCATCAGGCGAA30.11450381679389314No Hit
GTCCAGCCCATCCACGCCGGCTGCG30.11450381679389314No Hit
CCCCAGTTCTTTCTTCCATGAACAA30.11450381679389314No Hit
CACAGGTGGTACGTAGCACCTGCCG30.11450381679389314No Hit
CAGGTGGTACGTAGCACCTGCCGAG30.11450381679389314No Hit
GCTCCCTTCCAATCTGCATATAGCT30.11450381679389314No Hit
ACATATTGCAATGCGATCCCCATCT30.11450381679389314No Hit
CCTGTGTGGTTGAAGGAAGTCGGAC30.11450381679389314No Hit
ACCACACAGGTGGTACGTAGCACCT30.11450381679389314No Hit
GTTTTTTGTGTGTGGGGCGGTTTCT30.11450381679389314No Hit
GCCTAGCAGAGAGGCTGCCCCATGT30.11450381679389314No Hit
TGTATATAGTGTGTATAGTCTTAAT30.11450381679389314No Hit
TTCCTGAGCTCCCTTCCAATCTGCA30.11450381679389314No Hit
ACGCTTGCCTTCTCTCCTCCTTTTA30.11450381679389314No Hit
ACTCTCATTCCACCTCGTGCTTCGC30.11450381679389314No Hit
GCAAAGGCGCGATCTAAGAGCTTTC30.11450381679389314No Hit
GAGAGAAAGTTAATGGAGGCAGGAG30.11450381679389314No Hit
ATCAACGCAGAGTACATGGGAGATG30.11450381679389314No Hit
GGCTTCTACACCTCTTAACTCCCAG30.11450381679389314No Hit
GCCTCTCTGCTAGGCGTTGCATGTG30.11450381679389314No Hit
GGAGAGCTATATGCAGATTGGAAGG30.11450381679389314No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers