##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063881_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2620
Sequences flagged as poor quality	0
Sequence length	25
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.36908396946565	32.0	32.0	32.0	32.0	32.0
2	30.915648854961834	32.0	32.0	32.0	32.0	32.0
3	31.002671755725192	32.0	32.0	32.0	32.0	32.0
4	30.786641221374047	32.0	32.0	32.0	32.0	32.0
5	31.037022900763358	32.0	32.0	32.0	32.0	32.0
6	34.73053435114504	36.0	36.0	36.0	36.0	36.0
7	34.50763358778626	36.0	36.0	36.0	32.0	36.0
8	34.359923664122135	36.0	36.0	36.0	32.0	36.0
9	34.72328244274809	36.0	36.0	36.0	32.0	36.0
10	34.30534351145038	36.0	36.0	36.0	32.0	36.0
11	34.65992366412214	36.0	36.0	36.0	32.0	36.0
12	34.43435114503817	36.0	36.0	36.0	32.0	36.0
13	34.58931297709923	36.0	36.0	36.0	32.0	36.0
14	34.35954198473282	36.0	36.0	36.0	32.0	36.0
15	34.416030534351144	36.0	36.0	36.0	32.0	36.0
16	34.337786259541986	36.0	36.0	36.0	32.0	36.0
17	34.20267175572519	36.0	36.0	36.0	32.0	36.0
18	34.28396946564885	36.0	36.0	36.0	32.0	36.0
19	34.29274809160305	36.0	36.0	36.0	32.0	36.0
20	34.413358778625955	36.0	36.0	36.0	32.0	36.0
21	34.336641221374045	36.0	36.0	36.0	32.0	36.0
22	34.31870229007634	36.0	36.0	36.0	32.0	36.0
23	34.37404580152672	36.0	36.0	36.0	32.0	36.0
24	34.121374045801524	36.0	36.0	36.0	32.0	36.0
25	33.994656488549616	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	1.0
11	0.0
12	1.0
13	0.0
14	0.0
15	1.0
16	2.0
17	1.0
18	1.0
19	3.0
20	3.0
21	2.0
22	7.0
23	10.0
24	13.0
25	15.0
26	20.0
27	26.0
28	53.0
29	53.0
30	102.0
31	103.0
32	163.0
33	211.0
34	477.0
35	1350.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.124474990454374	19.320351279114163	11.263841160748377	23.291332569683085
2	20.72519083969466	18.62595419847328	36.52671755725191	24.12213740458015
3	22.862595419847327	22.824427480916032	27.022900763358777	27.29007633587786
4	17.480916030534353	14.465648854961833	35.49618320610687	32.55725190839694
5	18.17487590683467	34.860633829705996	31.04238258877434	15.922107674684993
6	33.33333333333333	33.90607101947308	17.869415807560138	14.891179839633448
7	31.89457601222307	30.099312452253628	18.71657754010695	19.28953399541635
8	30.989682842949946	30.034390523500193	19.105846388995033	19.870080244554835
9	29.128440366972473	16.743119266055047	17.16360856269113	36.964831804281346
10	20.14525993883792	24.273700305810397	27.522935779816514	28.05810397553517
11	37.62906309751434	23.021032504780116	20.994263862332694	18.35564053537285
12	29.44550669216061	22.179732313575524	24.091778202676863	24.282982791587
13	31.154434250764524	18.616207951070336	25.382262996941897	24.84709480122324
14	26.52905198776758	17.46941896024465	23.12691131498471	32.87461773700306
15	28.680688336520078	27.342256214149142	20.114722753346083	23.862332695984705
16	29.34200459066565	27.390971690895178	18.668706962509564	24.59831675592961
17	27.724665391969406	28.910133843212236	21.87380497131931	21.491395793499045
18	29.44550669216061	21.261950286806883	27.877629063097515	21.414913957934992
19	31.66347992351816	22.600382409177822	21.988527724665392	23.747609942638622
20	30.019120458891013	23.479923518164437	23.518164435946463	22.98279158699809
21	28.15608263198164	22.34123947972456	25.13389441469013	24.36878347360367
22	31.66347992351816	24.206500956022943	23.21223709369025	20.91778202676864
23	31.12810707456979	19.54110898661568	23.977055449330784	25.353728489483746
24	26.71259089169537	23.230003827018752	26.061997703788748	23.99540757749713
25	26.15678776290631	22.715105162523898	24.665391969407267	26.462715105162527
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1.0
1	1.0
2	0.5
3	0.0
4	0.0
5	0.0
6	1.0
7	2.0
8	2.0
9	2.0
10	1.5
11	1.0
12	1.0
13	1.0
14	5.0
15	9.0
16	9.0
17	9.0
18	8.5
19	8.0
20	8.0
21	8.0
22	17.0
23	26.0
24	26.0
25	26.0
26	63.5
27	101.0
28	101.0
29	101.0
30	95.5
31	90.0
32	90.0
33	90.0
34	104.0
35	118.0
36	118.0
37	118.0
38	139.0
39	160.0
40	160.0
41	160.0
42	175.5
43	191.0
44	191.0
45	191.0
46	294.0
47	397.0
48	397.0
49	397.0
50	428.0
51	459.0
52	459.0
53	459.0
54	376.0
55	293.0
56	293.0
57	293.0
58	294.0
59	295.0
60	295.0
61	295.0
62	238.0
63	181.0
64	181.0
65	181.0
66	129.0
67	77.0
68	77.0
69	77.0
70	68.5
71	60.0
72	60.0
73	60.0
74	42.0
75	24.0
76	24.0
77	24.0
78	19.5
79	15.0
80	15.0
81	15.0
82	11.5
83	8.0
84	8.0
85	8.0
86	5.0
87	2.0
88	2.0
89	2.0
90	1.5
91	1.0
92	1.0
93	1.0
94	2.0
95	3.0
96	3.0
97	3.0
98	50.5
99	98.0
100	98.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03816793893129771
2	0.0
3	0.0
4	0.0
5	0.03816793893129771
6	0.03816793893129771
7	0.07633587786259542
8	0.11450381679389314
9	0.15267175572519084
10	0.15267175572519084
11	0.19083969465648853
12	0.19083969465648853
13	0.15267175572519084
14	0.15267175572519084
15	0.19083969465648853
16	0.22900763358778628
17	0.19083969465648853
18	0.19083969465648853
19	0.19083969465648853
20	0.19083969465648853
21	0.22900763358778628
22	0.19083969465648853
23	0.19083969465648853
24	0.26717557251908397
25	0.19083969465648853
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2620.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.23664122137404
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.8659793814433	46.717557251908396
2	10.631443298969073	12.595419847328243
3	3.4149484536082473	6.068702290076336
4	2.384020618556701	5.648854961832061
5	0.902061855670103	2.6717557251908395
6	1.3530927835051547	4.809160305343512
7	0.3865979381443299	1.6030534351145038
8	0.3865979381443299	1.8320610687022902
9	0.6443298969072164	3.435114503816794
>10	0.9664948453608248	10.954198473282442
>50	0.06443298969072164	3.6641221374045805
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGGGGGGGGGGGGGGGGGGGGGGGG	96	3.6641221374045805	No Hit
ATCTAAGAGCTTTCGCCTGATGGTC	49	1.870229007633588	No Hit
GTACATGGGAGATGAGAGAAAGTTA	31	1.183206106870229	No Hit
CTATATACATGGCTTAATTACTCTT	24	0.9160305343511451	No Hit
CTCTTAGATCGCGCCTTTGCTCTCT	23	0.8778625954198473	No Hit
GTGTTGGCCCGCTCAATGCGTTCCA	21	0.8015267175572519	No Hit
GCATATAGCTCTCCACAGCAACTCT	21	0.8015267175572519	No Hit
ATCTTCGTAGAGAGCAAAGGCGCGA	20	0.7633587786259541	No Hit
GCTATATGCAGATTGGAAGGGAGCT	17	0.648854961832061	No Hit
ATGTCAACATCGCGCGAGATCTTGC	14	0.5343511450381679	No Hit
TGTCAACATCGCGCGAGATCTTGCT	13	0.4961832061068702	No Hit
CTACGAAGATAGCGTGGGCGCCATT	12	0.45801526717557256	No Hit
ACGTACCACCTGTGTGGTTGAAGGA	11	0.41984732824427484	No Hit
GATCAGGAGTCCTTCATCCGCGGAC	11	0.41984732824427484	No Hit
TCCTTCATCCGCGGACTGGTGAAGT	10	0.38167938931297707	No Hit
GCAATATGTCAACATCGCGCGAGAT	10	0.38167938931297707	No Hit
ATGTTGACATATTGCAATGCGATCC	9	0.3435114503816794	No Hit
ACGTAGCACCTGCCGAGTCTCGCAT	9	0.3435114503816794	No Hit
ATTCAGAAGAGTCTTCGTGGCCTGG	9	0.3435114503816794	No Hit
CCTTTTACATTTCCCTTCCTGAGCT	9	0.3435114503816794	No Hit
GGTGAAGTCCGACTTGGCCGAAGAC	9	0.3435114503816794	No Hit
CTCCTGATCAAGTCCGACTTCCTTC	9	0.3435114503816794	No Hit
GTGCTACGTACCACCTGTGTGGTTG	9	0.3435114503816794	No Hit
TATCAACGCAGAGTACATGGGAGAT	9	0.3435114503816794	No Hit
CGTACCACCTGTGTGGTTGAAGGAA	9	0.3435114503816794	No Hit
GGTGGTACGTAGCACCTGCCGAGTC	9	0.3435114503816794	No Hit
TCCTTCAACCACACAGGTGGTACGT	8	0.3053435114503817	No Hit
GCTCTCCACAGCAACTCTCATTCCA	8	0.3053435114503817	No Hit
GTTCCAGGCCACGAAGACTCTTCTG	8	0.3053435114503817	No Hit
GATTGGAAGGGAGCTCAGGAAGGGA	8	0.3053435114503817	No Hit
GATTAAGACTATACACACTATATAC	8	0.3053435114503817	No Hit
AAGATAGCGTGGGCGCCATTGAGCG	8	0.3053435114503817	No Hit
GACTGGTGAAGTCCGACTTGGCCGA	7	0.26717557251908397	No Hit
GTCCTTCATCCGCGGACTGGTGAAG	7	0.26717557251908397	No Hit
GTTGAAGGAAGTCGGACTTGATCAG	7	0.26717557251908397	No Hit
GTATCAACGCAGAGTACATGGGAGA	7	0.26717557251908397	No Hit
CACGCTATCTTCGTAGAGAGCAAAG	7	0.26717557251908397	No Hit
GCATTGCAATATGTCAACATCGCGC	7	0.26717557251908397	No Hit
CCATTGAGCGACTTCCCAGCGAAGC	6	0.22900763358778628	No Hit
CTTCTGAATGCAGGAACGCTTGCCT	6	0.22900763358778628	No Hit
CTCCACAGCAACTCTCATTCCACCT	6	0.22900763358778628	No Hit
GCTGTGGAGAGCTATATGCAGATTG	6	0.22900763358778628	No Hit
GAGCTCAGGAAGGGAAATGTAAAAG	6	0.22900763358778628	No Hit
CTATTAGGGTTGTGAGTGTCCTGGG	6	0.22900763358778628	No Hit
TCTGAATGCAGGAACGCTTGCCTTC	6	0.22900763358778628	No Hit
GATAGCGTGGGCGCCATTGAGCGAC	6	0.22900763358778628	No Hit
GTACCGGACTGGGCCGGTACCTCCC	6	0.22900763358778628	No Hit
AGTTAATGGAGGCAGGAGGCAAGAT	6	0.22900763358778628	No Hit
CACTATATACATGGCTTAATTACTC	6	0.22900763358778628	No Hit
GCTCTCTACGAAGATAGCGTGGGCG	6	0.22900763358778628	No Hit
CTCTCATTCCACCTCGTGCTTCGCT	6	0.22900763358778628	No Hit
ACGCAGAGTACATGGGAGATGAGAG	6	0.22900763358778628	No Hit
GTCCGCGGATGAAGGACTCCTGATC	6	0.22900763358778628	No Hit
TATCAACGCAGAGTACTTTTTTTTT	6	0.22900763358778628	No Hit
ACTATACACACTATATACATGGCTT	6	0.22900763358778628	No Hit
CTTCCAATCTGCATATAGCTCTCCA	6	0.22900763358778628	No Hit
GATGAAGGACTCCTGATCAAGTCCG	6	0.22900763358778628	No Hit
ATATACATGGCTTAATTACTCTTGA	6	0.22900763358778628	No Hit
TCCCTGACCTGTGCATACCACATCC	6	0.22900763358778628	No Hit
GTGGAGAGCTATATGCAGATTGGAA	5	0.19083969465648853	No Hit
CGCTATCTTCGTAGAGAGCAAAGGC	5	0.19083969465648853	No Hit
CTATACACACTATATACATGGCTTA	5	0.19083969465648853	No Hit
GATCGAGACCATCAGGCGAAAGCTC	5	0.19083969465648853	No Hit
GTACGTAGCACCTGCCGAGTCTCGC	5	0.19083969465648853	No Hit
ATCTTGCTGTCGATGCGAGACTCGG	5	0.19083969465648853	No Hit
CCTTTGCTCTCTACGAAGATAGCGT	5	0.19083969465648853	No Hit
GCTCAATGCGTTCCAGGCCACGAAG	5	0.19083969465648853	No Hit
CATTGCAATATGTCAACATCGCGCG	5	0.19083969465648853	No Hit
CAGAAGAGTCTTCGTGGCCTGGAAC	5	0.19083969465648853	No Hit
GGGCCGGGACTGCTGCAAGCGGTTC	5	0.19083969465648853	No Hit
TGTAAAAGGAGGAGAGAAGGCAAGC	5	0.19083969465648853	No Hit
TTGGAAGGGAGCTCAGGAAGGGAAA	5	0.19083969465648853	No Hit
GTGGAGCCGGGCGGGAACCTGTCAG	5	0.19083969465648853	No Hit
TTCCTGCATTCAGAAGAGTCTTCGT	4	0.15267175572519084	No Hit
TAGCTCTCCACAGCAACTCTCATTC	4	0.15267175572519084	No Hit
GAGTTGCTGTGGAGAGCTATATGCA	4	0.15267175572519084	No Hit
TCCGCGGACTGGTGAAGTCCGACTT	4	0.15267175572519084	No Hit
GTATCAACGCAGAGTACATGGGGGA	4	0.15267175572519084	No Hit
TCGCTGGGAAGTCGCTCAATGGCGC	4	0.15267175572519084	No Hit
GCTACGTACCACCTGTGTGGTTGAA	4	0.15267175572519084	No Hit
GGAGTCCTTCATCCGCGGACTGGTG	4	0.15267175572519084	No Hit
GAGCAAAGGCGCGATCTAAGAGCTT	4	0.15267175572519084	No Hit
GGCAAGATGGGGATCGCATTGCAAT	4	0.15267175572519084	No Hit
TCCCAGCGAAGCACGAGGTGGAATG	4	0.15267175572519084	No Hit
ATACATGGCTTAATTACTCTTGAGA	4	0.15267175572519084	No Hit
CTGGGAAGTCGCTCAATGGCGCCCA	4	0.15267175572519084	No Hit
CTTCCATGCTCGCCGCAGGTTCTCT	4	0.15267175572519084	No Hit
GATCTTGTCTTCGGCCAAGTCGGAC	4	0.15267175572519084	No Hit
GGACTTGGCACCTCATTGTTCATGG	4	0.15267175572519084	No Hit
ATACTATACGGCGACCGCTTCACCC	4	0.15267175572519084	No Hit
GGCGCGATCTAAGAGCTTTCGCCTG	4	0.15267175572519084	No Hit
GGCGGGAACCTGTCAGCGACGAAGC	4	0.15267175572519084	No Hit
GGTTTAGGGAGAGTGGACAGGACTT	4	0.15267175572519084	No Hit
TCACCAGTCCGCGGATGAAGGACTC	4	0.15267175572519084	No Hit
GGCCAACACCTCGGAAAGATCTCAA	4	0.15267175572519084	No Hit
TTGCAATATGTCAACATCGCGCGAG	4	0.15267175572519084	No Hit
TTCTGAATCTGTCCTCGTGTCGTTC	4	0.15267175572519084	No Hit
GGCTGGCCTTGCGCAACATCACCGC	4	0.15267175572519084	No Hit
CCACCTGTGTGGTTGAAGGAAGTCG	4	0.15267175572519084	No Hit
CCTTCAACCACACAGGTGGTACGTA	4	0.15267175572519084	No Hit
AGATCTTGCTGTCGATGCGAGACTC	4	0.15267175572519084	No Hit
GGTTGAAGGAAGTCGGACTTGATCA	4	0.15267175572519084	No Hit
CTGGTGAAGTCCGACTTGGCCGAAG	4	0.15267175572519084	No Hit
CAACCACACAGGTGGTACGTAGCAC	4	0.15267175572519084	No Hit
GGGGAGGTACCGGCCCAGTCCGGTA	4	0.15267175572519084	No Hit
GAATGAGAGTTGCTGTGGAGAGCTA	4	0.15267175572519084	No Hit
ACTATACGGCGACCGCTTCACCCTC	4	0.15267175572519084	No Hit
GGGTAATCAGAGGCCAGTCTCTGTC	4	0.15267175572519084	No Hit
CTTGAGATCTTTCCGAGGTGTTGGC	4	0.15267175572519084	No Hit
GCCCAGTCCGGTACGACGGGTAACG	4	0.15267175572519084	No Hit
CGGCAGGTGCTACGTACCACCTGTG	3	0.11450381679389314	No Hit
ACCTCGTGCTTCGCTGGGAAGTCGC	3	0.11450381679389314	No Hit
GTCTCGATCTTGTCTTCGGCCAAGT	3	0.11450381679389314	No Hit
ATCTGGTGACGGCGTGGCCATTGAT	3	0.11450381679389314	No Hit
CCACAGCAACTCTCATTCCACCTCG	3	0.11450381679389314	No Hit
GACTATACACACTATATACATGGCT	3	0.11450381679389314	No Hit
AGGCAAGATGGGGATCGCATTGCAA	3	0.11450381679389314	No Hit
TCGTTACCCGTCGTACCGGACTGGG	3	0.11450381679389314	No Hit
GTATCAACGCAGAGTACATGGGGCA	3	0.11450381679389314	No Hit
GCACTAATGGAAGCGGGCTCAGGAC	3	0.11450381679389314	No Hit
GAGTGGAAATCGGCGATGAACCAGT	3	0.11450381679389314	No Hit
GTACATGGGGCACTTCCCAAGCGAC	3	0.11450381679389314	No Hit
GGTCAATGTCCAGCCCATCCACGCC	3	0.11450381679389314	No Hit
AAGACTCTTCTGAATGCAGGAACGC	3	0.11450381679389314	No Hit
CTCTACGAAGATAGCGTGGGCGCCA	3	0.11450381679389314	No Hit
TTACTCTTGAGATCTTTCCGAGGTG	3	0.11450381679389314	No Hit
TTCCAGGCCACGAAGACTCTTCTGA	3	0.11450381679389314	No Hit
GAGCTATATGCAGATTGGAAGGGAG	3	0.11450381679389314	No Hit
CAGCAACTCTCATTCCACCTCGTGC	3	0.11450381679389314	No Hit
ATCGAGACCATCAGGCGAAAGCTCT	3	0.11450381679389314	No Hit
AGACTATACACACTATATACATGGC	3	0.11450381679389314	No Hit
AAACAGGACATCCCCTCGGAATGCC	3	0.11450381679389314	No Hit
GAATGCAGGAACGCTTGCCTTCTCT	3	0.11450381679389314	No Hit
AGGTGGAATGAGAGTTGCTGTGGAG	3	0.11450381679389314	No Hit
CTCATTCCACCTCGTGCTTCGCTGG	3	0.11450381679389314	No Hit
AAGGACTCCTGATCAAGTCCGACTT	3	0.11450381679389314	No Hit
GCTCAGGACAGGTAAGTTTTGATAG	3	0.11450381679389314	No Hit
GTATAGTATCGCGAACTGGTTCATC	3	0.11450381679389314	No Hit
TGCCTTCTCTCCTCCTTTTACATTT	3	0.11450381679389314	No Hit
AGACAAGATCGAGACCATCAGGCGA	3	0.11450381679389314	No Hit
GTCCGGTACGACGGGTAACGATCCC	3	0.11450381679389314	No Hit
CCCTAAACCTCTCTTGAGTCTGCAT	3	0.11450381679389314	No Hit
GACAAGATCGAGACCATCAGGCGAA	3	0.11450381679389314	No Hit
GTCCAGCCCATCCACGCCGGCTGCG	3	0.11450381679389314	No Hit
CCCCAGTTCTTTCTTCCATGAACAA	3	0.11450381679389314	No Hit
CACAGGTGGTACGTAGCACCTGCCG	3	0.11450381679389314	No Hit
CAGGTGGTACGTAGCACCTGCCGAG	3	0.11450381679389314	No Hit
GCTCCCTTCCAATCTGCATATAGCT	3	0.11450381679389314	No Hit
ACATATTGCAATGCGATCCCCATCT	3	0.11450381679389314	No Hit
CCTGTGTGGTTGAAGGAAGTCGGAC	3	0.11450381679389314	No Hit
ACCACACAGGTGGTACGTAGCACCT	3	0.11450381679389314	No Hit
GTTTTTTGTGTGTGGGGCGGTTTCT	3	0.11450381679389314	No Hit
GCCTAGCAGAGAGGCTGCCCCATGT	3	0.11450381679389314	No Hit
TGTATATAGTGTGTATAGTCTTAAT	3	0.11450381679389314	No Hit
TTCCTGAGCTCCCTTCCAATCTGCA	3	0.11450381679389314	No Hit
ACGCTTGCCTTCTCTCCTCCTTTTA	3	0.11450381679389314	No Hit
ACTCTCATTCCACCTCGTGCTTCGC	3	0.11450381679389314	No Hit
GCAAAGGCGCGATCTAAGAGCTTTC	3	0.11450381679389314	No Hit
GAGAGAAAGTTAATGGAGGCAGGAG	3	0.11450381679389314	No Hit
ATCAACGCAGAGTACATGGGAGATG	3	0.11450381679389314	No Hit
GGCTTCTACACCTCTTAACTCCCAG	3	0.11450381679389314	No Hit
GCCTCTCTGCTAGGCGTTGCATGTG	3	0.11450381679389314	No Hit
GGAGAGCTATATGCAGATTGGAAGG	3	0.11450381679389314	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE