FastQCFastQC Report
Thu 2 Feb 2017
SRR4063881_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063881_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2620
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATATACATGGCTTAATTACTCTTGAGATCTTTCCGAGGTGTTGGCCCG391.4885496183206106No Hit
ATCTAAGAGCTTTCGCCTGATGGTCTCGATCTTGTCTTCGGCCAAGTCGG341.297709923664122No Hit
GTACATGGGAGATGAGAGAAAGTTAATGGAGGCAGGAGGCAAGATGGGGA311.183206106870229No Hit
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGT301.1450381679389312No Hit
GCATATAGCTCTCCACAGCAACTCTCATTCCACCTCGTGCTTCGCTGGGA271.0305343511450382No Hit
GTGTTGGCCCGCTCAATGCGTTCCAGGCCACGAAGACTCTTCTGAATGCA230.8778625954198473No Hit
CTACGAAGATAGCGTGGGCGCCATTGAGCGACTTCCCAGCGAAGCACGAG210.8015267175572519No Hit
ATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTT200.7633587786259541TruSeq Adapter, Index 11 (95% over 23bp)
ATCTTCGTAGAGAGCAAAGGCGCGATCTAAGAGCTTTCGCCTGATGGTCT180.6870229007633588No Hit
GCTATATGCAGATTGGAAGGGAGCTCAGGAAGGGAAATGTAAAAGGAGGA160.6106870229007634No Hit
TGTCAACATCGCGCGAGATCTTGCTGTCGATGCGAGACTCGGCAGGTGCT150.5725190839694656No Hit
ACGTACCACCTGTGTGGTTGAAGGAAGTCGGACTTGATCAGGAGTCCTTC140.5343511450381679No Hit
ACGTAGCACCTGCCGAGTCTCGCATCGACAGCAAGATCTCGCGCGATGTT140.5343511450381679No Hit
GATCAGGAGTCCTTCATCCGCGGACTGGTGAAGTCCGACTTGGCCGAAGA130.4961832061068702No Hit
GTGCTACGTACCACCTGTGTGGTTGAAGGAAGTCGGACTTGATCAGGAGT130.4961832061068702No Hit
CTCTTAGATCGCGCCTTTGCTCTCTACGAAGATAGCGTGGGCGCCATTGA130.4961832061068702No Hit
TCCTTCAACCACACAGGTGGTACGTAGCACCTGCCGAGTCTCGCATCGAC120.45801526717557256No Hit
GCAATATGTCAACATCGCGCGAGATCTTGCTGTCGATGCGAGACTCGGCA120.45801526717557256No Hit
CTTCTGAATGCAGGAACGCTTGCCTTCTCTCCTCCTTTTACATTTCCCTT110.41984732824427484No Hit
AGTTAATGGAGGCAGGAGGCAAGATGGGGATCGCATTGCAATATGTCAAC100.38167938931297707No Hit
CACTATATACATGGCTTAATTACTCTTGAGATCTTTCCGAGGTGTTGGCC100.38167938931297707No Hit
ACGCAGAGTACATGGGAGATGAGAGAAAGTTAATGGAGGCAGGAGGCAAG90.3435114503816794No Hit
CTCTCATTCCACCTCGTGCTTCGCTGGGAAGTCGCTCAATGGCGCCCACG90.3435114503816794No Hit
ATGTCAACATCGCGCGAGATCTTGCTGTCGATGCGAGACTCGGCAGGTGC90.3435114503816794No Hit
ATACTATACGGCGACCGCTTCACCCTCGCACCACGCTGAACGACACGAGG80.3053435114503817No Hit
ACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCT80.3053435114503817RNA PCR Primer, Index 11 (96% over 25bp)
ATTCAGAAGAGTCTTCGTGGCCTGGAACGCATTGAGCGGGCCAACACCTC80.3053435114503817No Hit
GATGAAGGACTCCTGATCAAGTCCGACTTCCTTCAACCACACAGGTGGTA80.3053435114503817No Hit
GAGCAAAGGCGCGATCTAAGAGCTTTCGCCTGATGGTCTCGATCTTGTCT80.3053435114503817No Hit
GACAAGATCGAGACCATCAGGCGAAAGCTCTTAGATCGCGCCTTTGCTCT80.3053435114503817No Hit
CTCCTGATCAAGTCCGACTTCCTTCAACCACACAGGTGGTACGTAGCACC80.3053435114503817No Hit
GGTGAAGTCCGACTTGGCCGAAGACAAGATCGAGACCATCAGGCGAAAGC70.26717557251908397No Hit
GTTGAAGGAAGTCGGACTTGATCAGGAGTCCTTCATCCGCGGACTGGTGA70.26717557251908397No Hit
GCTTAATTACTCTTGAGATCTTTCCGAGGTGTTGGCCCGCTCAATGCGTT70.26717557251908397No Hit
GGAGTCCTTCATCCGCGGACTGGTGAAGTCCGACTTGGCCGAAGACAAGA70.26717557251908397No Hit
TATCAACGCAGAGTACATGGGAGATGAGAGAAAGTTAATGGAGGCAGGAG70.26717557251908397No Hit
GTATCAACGCAGAGTACATGGGAGATGAGAGAAAGTTAATGGAGGCAGGA70.26717557251908397No Hit
CCCTTCCAATCTGCATATAGCTCTCCACAGCAACTCTCATTCCACCTCGT70.26717557251908397No Hit
AGATCTTGCTGTCGATGCGAGACTCGGCAGGTGCTACGTACCACCTGTGT70.26717557251908397No Hit
CTATACACACTATATACATGGCTTAATTACTCTTGAGATCTTTCCGAGGT60.22900763358778628No Hit
GCATTGCAATATGTCAACATCGCGCGAGATCTTGCTGTCGATGCGAGACT60.22900763358778628No Hit
CATTGCAATATGTCAACATCGCGCGAGATCTTGCTGTCGATGCGAGACTC60.22900763358778628No Hit
CCATTGAGCGACTTCCCAGCGAAGCACGAGGTGGAATGAGAGTTGCTGTG60.22900763358778628No Hit
CTCCACAGCAACTCTCATTCCACCTCGTGCTTCGCTGGGAAGTCGCTCAA60.22900763358778628No Hit
TCACCAGTCCGCGGATGAAGGACTCCTGATCAAGTCCGACTTCCTTCAAC60.22900763358778628No Hit
TAGCTCTCCACAGCAACTCTCATTCCACCTCGTGCTTCGCTGGGAAGTCG60.22900763358778628No Hit
GGCCCGCTCAATGCGTTCCAGGCCACGAAGACTCTTCTGAATGCAGGAAC60.22900763358778628No Hit
AAGATAGCGTGGGCGCCATTGAGCGACTTCCCAGCGAAGCACGAGGTGGA60.22900763358778628No Hit
CGTACCACCTGTGTGGTTGAAGGAAGTCGGACTTGATCAGGAGTCCTTCA60.22900763358778628No Hit
ATATAGCTCTCCACAGCAACTCTCATTCCACCTCGTGCTTCGCTGGGAAG50.19083969465648853No Hit
GTTAATGGAGGCAGGAGGCAAGATGGGGATCGCATTGCAATATGTCAACA50.19083969465648853No Hit
ACTATACACACTATATACATGGCTTAATTACTCTTGAGATCTTTCCGAGG50.19083969465648853No Hit
GTCCGGTACGACGGGTAACGATCCCGAAGCCGGACGGTGGCGAGCGCGAG50.19083969465648853No Hit
GCTCAATGCGTTCCAGGCCACGAAGACTCTTCTGAATGCAGGAACGCTTG50.19083969465648853No Hit
CGCTATCTTCGTAGAGAGCAAAGGCGCGATCTAAGAGCTTTCGCCTGATG50.19083969465648853No Hit
CTCATTCCACCTCGTGCTTCGCTGGGAAGTCGCTCAATGGCGCCCACGCT50.19083969465648853No Hit
GGTGGTACGTAGCACCTGCCGAGTCTCGCATCGACAGCAAGATCTCGCGC50.19083969465648853No Hit
ATATACATGGCTTAATTACTCTTGAGATCTTTCCGAGGTGTTGGCCCGCT50.19083969465648853No Hit
ATGCAGGAACGCTTGCCTTCTCTCCTCCTTTTACATTTCCCTTCCTGAGC50.19083969465648853No Hit
GATCGAGACCATCAGGCGAAAGCTCTTAGATCGCGCCTTTGCTCTCTACG50.19083969465648853No Hit
AGACAAGATCGAGACCATCAGGCGAAAGCTCTTAGATCGCGCCTTTGCTC50.19083969465648853No Hit
GCTCAATGGCGCCCACGCTATCTTCGTAGAGAGCAAAGGCGCGATCTAAG50.19083969465648853No Hit
TCCTTCATCCGCGGACTGGTGAAGTCCGACTTGGCCGAAGACAAGATCGA50.19083969465648853No Hit
GAATAGTTCAGTCTCTGATAACATCTGCAAGAACTTACTGCTTCAGGGTA50.19083969465648853No Hit
TTGCAATATGTCAACATCGCGCGAGATCTTGCTGTCGATGCGAGACTCGG50.19083969465648853No Hit
CCCTAATAGCATCGCATCCTAGTCAGATGAGCCAGGCTTCTCATAGAGCA40.15267175572519084No Hit
CCTGAGCTCCCTTCCAATCTGCATATAGCTCTCCACAGCAACTCTCATTC40.15267175572519084No Hit
ACTATACGGCGACCGCTTCACCCTCGCACCACGCTGAACGACACGAGGAC40.15267175572519084No Hit
CCTGTGTGGTTGAAGGAAGTCGGACTTGATCAGGAGTCCTTCATCCGCGG40.15267175572519084No Hit
GTACATGGGGGTAGCGCACGGTGGAGCCGGGCGGGAACCTGTCAGCGACG40.15267175572519084No Hit
GCTCTCCACAGCAACTCTCATTCCACCTCGTGCTTCGCTGGGAAGTCGCT40.15267175572519084No Hit
GATCAGGAGTCCTTCATCCGCGGACTGGTGAAGTCCGACTTGGTCGAAGA40.15267175572519084No Hit
GTTCTGGCGAGAGAGAACCTGCGGCGAGCATGGAAGAGAGTCAAAGCCAA40.15267175572519084No Hit
GAATGAGAGTTGCTGTGGAGAGCTATATGCAGATTGGAAGGGAGCTCAGG40.15267175572519084No Hit
GGTTTAGGGAGAGTGGACAGGACTTGGCACCTCATTGTTCATGGAAGAAA40.15267175572519084No Hit
GCCCAGTCCGGTACGACGGGTAACGATCCCGAAGCCGGACGGTGGCGAGC40.15267175572519084No Hit
GATCAAGTCCGACTTCCTTCAACCACACAGGTGGTACGTAGCACCTGCCG40.15267175572519084No Hit
GTATCAACGCAGAGTACATGGGGCACTTCCCAAGCGACGAGGCGGCCACC40.15267175572519084No Hit
GACTTGATCAGGAGTCCTTCATCCGCGGACTGGTGAAGTCCGACTTGGCC40.15267175572519084No Hit
CATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCT40.15267175572519084TruSeq Adapter, Index 11 (96% over 28bp)
ACGCAGAGTACATGGGGGTAGCGCACGGTGGAGCCGGGCGGGAACCTGTC40.15267175572519084No Hit
ATACATGGCTTAATTACTCTTGAGATCTTTCCGAGGTGTTGGCCCGCTCA40.15267175572519084No Hit
GTCTCGATCTTGTCTTCGGCCAAGTCGGACTTCACCAGTCCGCGGATGAA40.15267175572519084No Hit
ATGCGTTCCAGGCCACGAAGACTCTTCTGAATGCAGGAACGCTTGCCTTC40.15267175572519084No Hit
AGACTATACACACTATATACATGGCTTAATTACTCTTGAGATCTTTCCGA40.15267175572519084No Hit
CCACCTGTGTGGTTGAAGGAAGTCGGACTTGATCAGGAGTCCTTCATCCG40.15267175572519084No Hit
TATGTCAACATCGCGCGAGATCTTGCTGTCGATGCGAGACTCGGCAGGTG40.15267175572519084No Hit
TTGGAAGGGAGCTCAGGAAGGGAAATGTAAAAGGAGGAGAGAAGGCAAGC40.15267175572519084No Hit
TTTCCCTTCCTGAGCTCCCTTCCAATCTGCATATAGCTCTCCACAGCAAC40.15267175572519084No Hit
GTGGAGAGCTATATGCAGATTGGAAGGGAGCTCAGGAAGGGAAATGTAAA40.15267175572519084No Hit
GACCAGACCGCCCGCCATCTGGTGACGGCGTGGCCATTGATTCGGGAACA40.15267175572519084No Hit
GGTATCAACGCAGAGTACATGGGAGATGAGAGAAAGTTAATGGAGGCAGG40.15267175572519084No Hit
GTCCAGCCCATCCACGCCGGCTGCGCCTTTGTTGGCTTTGACTCTCTTCC40.15267175572519084No Hit
TTTCCGAGGTGTTGGCCCGCTCAATGCGTTCCAGGCCACGAAGACTCTTC40.15267175572519084No Hit
TGTCAGAACCCCATGTCTGGCTCTAGGGATTAAAGTGATCATCCTGACAG40.15267175572519084No Hit
TATCAACGCAGAGTACATGGGGGAGGCCTGTGCCACCCTGGCAGGGGAAT40.15267175572519084No Hit
GCCCGCGACGGGACACCGAGAGCACAGGGCCGGGACTGCTGCAAGCGGTT30.11450381679389314No Hit
GTACATGGGGCACTTCCCAAGCGACGAGGCGGCCACCAAGCTGATCTGGC30.11450381679389314No Hit
GCTTTCGCCTGATGGTCTCGATCTTGTCTTCGGCCAAGTCGGACTTCACC30.11450381679389314No Hit
CGATCTTGTCTTCGGCCAAGTCGGACTTCACCAGTCCGCGGATGAAGGAC30.11450381679389314No Hit
CCGTATAGTATCGCGAACTGGTTCATCGCCGATTTCCACTCCTTGCTCGC30.11450381679389314No Hit
GACTGGTGAAGTCCGACTTGGCCGAAGACAAGATCGAGACCATCAGGCGA30.11450381679389314No Hit
AGGCAAGCGTTCCTGCATTCAGAAGAGTCTTCGTGGCCTGGAACGCATTG30.11450381679389314No Hit
GTTCCAGGCCACGAAGACTCTTCTGAATGCAGGAACGCTTGCCTTCTCTC30.11450381679389314No Hit
TCCCAGCGAAGCACGAGGTGGAATGAGAGTTGCTGTGGAGAGCTATATGC30.11450381679389314No Hit
GCAACTCTCATTCCACCTCGTGCTTCGCTGGGAAGTCGCTCAATGGCGCC30.11450381679389314No Hit
CCTTTTACATTTCCCTTCCTGAGCTCCCTTCCAATCTGCATATAGCTCTC30.11450381679389314No Hit
GATGGGGATCGCATTGCAATATGTCAACATCGCGCGAGATCTTGCTGTCG30.11450381679389314No Hit
GGCAAGCGTTCCTGCATTCAGAAGAGTCTTCGTGGCCTGGAACGCATTGA30.11450381679389314No Hit
GGGGATCGCATTGCAATATGTCAACATCGCGCGAGATCTTGCTGTCGATG30.11450381679389314No Hit
CTTTAATCCCTAGAGCCAGACATGGGGTTCTGACATGGGGCAGCCTCTCT30.11450381679389314No Hit
GTAAAAGGAGGAGAGAAGGCAAGCGTTCCTGCATTCAGAAGAGTCTTCGT30.11450381679389314No Hit
CTGCTAGGCGTTGCATGTGTTCTTTGGAAGGGAAAGATGATCGGCACCTT30.11450381679389314No Hit
TCGCTGGGAAGTCGCTCAATGGCGCCCACGCTATCTTCGTAGAGAGCAAA30.11450381679389314No Hit
CTTTCCGAGGTGTTGGCCCGCTCAATGCGTTCCAGGCCACGAAGACTCTT30.11450381679389314No Hit
GTGATCATCCTGACAGATGCTCAGGGCAACACCTTCTTGGCATCCCCAGT30.11450381679389314No Hit
GCCGAGTCTCGCATCGACAGCAAGATCTCGCGCGATGTTGACATATTGCA30.11450381679389314No Hit
GGGCCGGGACTGCTGCAAGCGGTTCTGGCGAGAGAGAACCTGCGGCGAGC30.11450381679389314No Hit
GGACTTGGCACCTCATTGTTCATGGAAGAAAGAACTGGGGATGCCAAGAA30.11450381679389314No Hit
CTTCCAATCTGCATATAGCTCTCCACAGCAACTCTCATTCCACCTCGTGC30.11450381679389314No Hit
ATGCGAGACTCGGCAGGTGCTACGTACCACCTGTGTGGTTGAAGGAAGTC30.11450381679389314No Hit
GATTGGAAGGGAGCTCAGGAAGGGAAATGTAAAAGGAGGAGAGAAGGCAA30.11450381679389314No Hit
ATGTCCACCTTTAATCACACCTGGACAGTAGCAGTGCTGAGGGAGGGATG30.11450381679389314No Hit
CCACAGCAACTCTCATTCCACCTCGTGCTTCGCTGGGAAGTCGCTCAATG30.11450381679389314No Hit
CCCTAGAGCCAGACATGGGGTTCTGACATGGGGCAGCCTCTCTGCTAGGC30.11450381679389314No Hit
CTTCATCCGCGGACTGGTGAAGTCCGACTTGGCCGAAGACAAGATCGAGA30.11450381679389314No Hit
GCTACGTACCACCTGTGTGGTTGAAGGAAGTCGGACTTGATCAGGAGTCC30.11450381679389314No Hit
ACATGGGAGATGAGAGAAAGTTAATGGAGGCAGGAGGCAAGATGGGGATC30.11450381679389314No Hit
CAAGATGGGGATCGCATTGCAATATGTCAACATCGCGCGAGATCTTGCTG30.11450381679389314No Hit
AGGCAAGATGGGGATCGCATTGCAATATGTCAACATCGCGCGAGATCTTG30.11450381679389314No Hit
CGGCAGGTGCTACGTACCACCTGTGTGGTTGAAGGAAGTCGGACTTGATC30.11450381679389314No Hit
TCTGGTGACGGCGTGGCCATTGATTCGGGAACAACTGCTGCAGGGGAGGT30.11450381679389314No Hit
AAGTTAATGGAGGCAGGAGGCAAGATGGGGATCGCATTGCAATATGTCAA30.11450381679389314No Hit
CTGGTGAAGTCCGACTTGGCCGAAGACAAGATCGAGACCATCAGGCGAAA30.11450381679389314No Hit
CACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTG30.11450381679389314TruSeq Adapter, Index 11 (96% over 26bp)
GAAGAGTCTTCGTGGCCTGGAACGCATTGAGCGGGCCAACACCTCGGAAA30.11450381679389314No Hit
TATCAACGCAGAGTACATGGGGCACTTCCCAAGCGACGAGGCGGCCACCA30.11450381679389314No Hit
CCTTTGCTCTCTACGAAGATAGCGTGGGCGCCATTGAGCGACTTCCCAGC30.11450381679389314No Hit
CATTAGTGCTCTATGAGAAGCCTGGCTCATCTGACTAGGATGCGATGCTA30.11450381679389314No Hit
TACCTGTCCTGAGCCCGCTTCCATTAGTGCTCTATGAGAAGCCTGGCTCA30.11450381679389314No Hit
TTCAGAAACCGCCCCACACACAAAAAACAGGACATCCCCTCGGAATGCCG30.11450381679389314No Hit
TACCACCTGTGTGGTTGAAGGAAGTCGGACTTGATCAGGAGTCCTTCATC30.11450381679389314No Hit
ATGTTGACATATTGCAATGCGATCCCCATCTTGCCTCCTGCCTCCATTAA30.11450381679389314No Hit
TCTCTCCTCCTTTTACATTTCCCTTCCTGAGCTCCCTTCCAATCTGCATA30.11450381679389314No Hit
TGTATATAGTGTGTATAGTCTTAATCATATTTTGAGGTCTCGAAAAAAAA30.11450381679389314No Hit
ACGCTGAACGACACGAGGACAGATTCAGAAACCGCCCCACACACAAAAAA30.11450381679389314No Hit
AAGGAGGAGAGAAGGCAAGCGTTCCTGCATTCAGAAGAGTCTTCGTGGCC30.11450381679389314No Hit
CTTCCATGCTCGCCGCAGGTTCTCTCTCGCCAGAACCGCTTGCAGCAGTC30.11450381679389314No Hit
GTCGATGCGAGACTCGGCAGGTGCTACGTACCACCTGTGTGGTTGAAGGA30.11450381679389314No Hit
TATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCT30.11450381679389314RNA PCR Primer, Index 11 (95% over 22bp)
AAACAGGACATCCCCTCGGAATGCCGAGCTCGCGCTCGCCACCGTCCGGC30.11450381679389314No Hit
GTGCATACCACATCCCATCCTGGCTTCTACACCTCTTAACTCCCAGGAGC30.11450381679389314No Hit
CCACACAGGTGGTACGTAGCACCTGCCGAGTCTCGCATCGACAGCAAGAT30.11450381679389314No Hit
ATCTTGCTGTCGATGCGAGACTCGGCAGGTGCTACGTACCACCTGTGTGG30.11450381679389314No Hit
GCTGTGGAGAGCTATATGCAGATTGGAAGGGAGCTCAGGAAGGGAAATGT30.11450381679389314No Hit
GACTTCACCAGTCCGCGGATGAAGGACTCCTGATCAAGTCCGACTTCCTT30.11450381679389314No Hit
CTGGGAAGTCGCTCAATGGCGCCCACGCTATCTTCGTAGAGAGCAAAGGC30.11450381679389314No Hit
CCTCGTGCTTCGCTGGGAAGTCGCTCAATGGCGCCCACGCTATCTTCGTA30.11450381679389314No Hit
TCCATGCTCGCCGCAGGTTCTCTCTCGCCAGAACCGCTTGCAGCAGTCCC30.11450381679389314No Hit
GTATCAACGCAGAGTACATGGGGGAGGCCTGTGCCACCCTGGCAGGGGAA30.11450381679389314No Hit
ATATGCAGATTGGAAGGGAGCTCAGGAAGGGAAATGTAAAAGGAGGAGAG30.11450381679389314No Hit
CAGGTGGTACGTAGCACCTGCCGAGTCTCGCATCGACAGCAAGATCTCGC30.11450381679389314No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAGCAA206.493948E-444.015
AGCTCTC206.493948E-444.07
CAACTCT206.493948E-444.019
CTCTCCA206.493948E-444.09
TAGCTCT206.493948E-444.06
CTCCACA206.493948E-444.011
ACTCTCA206.493948E-444.021
AACTCTC206.493948E-444.020
ATAGCTC206.493948E-444.05
CCACAGC206.493948E-444.013
TCCACAG206.493948E-444.012
TCTCCAC206.493948E-444.010
GCTCTCC206.493948E-444.08
CATATAG206.493948E-444.02
CACAGCA206.493948E-444.014
TATAGCT206.493948E-444.04
GCATATA206.493948E-444.01
ATATAGC206.493948E-444.03
CGCTGGG250.001948177535.243
CAGCAAC250.001948177535.216