FastQCFastQC Report
Thu 2 Feb 2017
SRR4063880_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063880_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3151621
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTAAAGTGTGTATTTCTCATTT52470.16648575447365022No Hit
GTCCTACAGTGGACATTTCTAAATT50600.16055229991169623No Hit
CTGTAGGACGTGGAATATGGCAAGA47710.15138241558867643No Hit
GTATCAACGCAGAGTACTTTTTTTT47610.1510651185532778No Hit
CTTTAGGACGTGAAATATGGCGAGG43120.13681848166388028No Hit
GTCCTACAGTGTGCATTTCTCATTT33730.10702429003995087No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10650.014.2684611
GTATCAA33150.013.0357081
TAGCGTG604.095717E-412.6660735
CCGTCGT4000.011.8757659
TTAGACC1052.7281203E-711.7602354
ATACCGT4300.011.7094976
CGAACGA2450.011.6341416
TCCAACG3200.011.5788718
TAGGACC14800.011.5524144
TAGAAAT8300.011.4441574
CGACCAT3000.011.40091410
ACCGTCG4350.011.1386498
CGTCGTA4200.011.08422210
GTCCTAT6350.011.0678881
CCGACCA3200.010.9850829
GATATAC5900.010.9462071
CTAGGAC4050.010.7872213
ACGAACG2650.010.75609215
TAACGAA2650.010.75540913
GATAACG2750.010.7099511
Produced by FastQC (version 0.11.5)