##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063880_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3151621
Sequences flagged as poor quality	0
Sequence length	25
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.241127660971927	32.0	32.0	32.0	32.0	32.0
2	30.779433186921903	32.0	32.0	32.0	32.0	32.0
3	30.79263528197077	32.0	32.0	32.0	32.0	32.0
4	30.764842600046133	32.0	32.0	32.0	32.0	32.0
5	30.70846526279651	32.0	32.0	32.0	32.0	32.0
6	34.15522107512293	36.0	36.0	36.0	32.0	36.0
7	34.13170873020582	36.0	36.0	36.0	32.0	36.0
8	34.05312028318126	36.0	36.0	36.0	32.0	36.0
9	34.312444611836256	36.0	36.0	36.0	32.0	36.0
10	33.88877628369655	36.0	36.0	36.0	32.0	36.0
11	34.30739387762679	36.0	36.0	36.0	32.0	36.0
12	34.044443795748286	36.0	36.0	36.0	32.0	36.0
13	34.18662745298372	36.0	36.0	36.0	32.0	36.0
14	34.01627130927228	36.0	36.0	36.0	32.0	36.0
15	33.91046797822454	36.0	36.0	36.0	32.0	36.0
16	33.964925668410004	36.0	36.0	36.0	32.0	36.0
17	33.84368361551088	36.0	36.0	36.0	32.0	36.0
18	33.8819366288015	36.0	36.0	36.0	32.0	36.0
19	33.87165430107237	36.0	36.0	36.0	32.0	36.0
20	33.82416318459612	36.0	36.0	36.0	32.0	36.0
21	33.803561722681756	36.0	36.0	36.0	32.0	36.0
22	33.76947481946592	36.0	36.0	36.0	32.0	36.0
23	33.71359119640338	36.0	36.0	36.0	32.0	36.0
24	33.69912911482694	36.0	36.0	36.0	32.0	36.0
25	32.94318606202967	36.0	32.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	6.0
4	25.0
5	39.0
6	119.0
7	52.0
8	150.0
9	179.0
10	240.0
11	104.0
12	156.0
13	156.0
14	437.0
15	1128.0
16	1798.0
17	2370.0
18	2815.0
19	3556.0
20	4467.0
21	5876.0
22	7938.0
23	11097.0
24	15400.0
25	21645.0
26	30484.0
27	43029.0
28	61180.0
29	87389.0
30	124879.0
31	187120.0
32	293220.0
33	444193.0
34	860256.0
35	940117.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.821108844064284	17.946726374501182	12.095280296287644	26.136884485146894
2	15.750198717104585	19.622842075034942	39.579560617046354	25.04739859081411
3	18.787388028279175	23.83121212015445	28.76826426896757	28.613135582598808
4	12.410908013217757	16.005842263950264	36.43409303072361	35.14915669210838
5	14.042622192365654	36.918406416525166	34.147446676247704	14.891524714861474
6	33.37984394773887	35.739604449279206	17.4674383387936	13.413113264188329
7	29.999406537418764	30.49490018879727	20.966176123826127	18.539517149957838
8	26.97445409421011	34.86341524260945	19.34429413831486	18.817836524865577
9	26.99589658258052	15.01608893880299	19.035419155185487	38.952595323431
10	15.773146457992732	26.9980476500373	32.1927588450977	25.036047046872273
11	35.993908731928656	20.94776550332166	24.115618540844796	18.942707223904893
12	25.30756431539617	23.25637359996368	29.631862555078385	21.804199529561764
13	29.22775903412495	20.304672635497983	25.493054018865287	24.974514311511783
14	22.677723230117504	20.891196519843046	25.444044164873137	30.987036085166313
15	24.015465563755292	29.48863575035413	22.105444947659752	24.390453738230825
16	24.440000330186614	26.13693727787807	25.516249943646034	23.906812448289283
17	23.018632274417534	27.051101049984172	26.136710333199254	23.793556342399036
18	23.66599500988434	25.312453761415572	28.072480509146747	22.94907071955334
19	25.355357993332273	24.949833306874105	25.669153833941895	24.025654865851724
20	25.361700587325863	25.338108484932963	25.701769788709385	23.59842113903178
21	25.360218652245642	24.74659276654275	24.892363047059256	25.000825534152348
22	25.187821787994203	25.92189928541831	25.230909428636295	23.659369497951193
23	23.343309174333776	25.81048018988497	26.229179731843043	24.617030903938208
24	24.562670227918872	25.310514139911234	26.225307624750194	23.9015080074197
25	24.009754331818744	25.531106221244247	26.320692709970565	24.13844673696644
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	127.0
1	127.0
2	350.0
3	573.0
4	573.0
5	573.0
6	1661.0
7	2749.0
8	2749.0
9	2749.0
10	3287.0
11	3825.0
12	3825.0
13	3825.0
14	5866.0
15	7907.0
16	7907.0
17	7907.0
18	14317.0
19	20727.0
20	20727.0
21	20727.0
22	36452.0
23	52177.0
24	52177.0
25	52177.0
26	82284.5
27	112392.0
28	112392.0
29	112392.0
30	146463.0
31	180534.0
32	180534.0
33	180534.0
34	211445.5
35	242357.0
36	242357.0
37	242357.0
38	266628.5
39	290900.0
40	290900.0
41	290900.0
42	319417.5
43	347935.0
44	347935.0
45	347935.0
46	376151.0
47	404367.0
48	404367.0
49	404367.0
50	403708.0
51	403049.0
52	403049.0
53	403049.0
54	371424.5
55	339800.0
56	339800.0
57	339800.0
58	308521.5
59	277243.0
60	277243.0
61	277243.0
62	241171.0
63	205099.0
64	205099.0
65	205099.0
66	166331.0
67	127563.0
68	127563.0
69	127563.0
70	96207.5
71	64852.0
72	64852.0
73	64852.0
74	49056.5
75	33261.0
76	33261.0
77	33261.0
78	26836.5
79	20412.0
80	20412.0
81	20412.0
82	13814.5
83	7217.0
84	7217.0
85	7217.0
86	5454.0
87	3691.0
88	3691.0
89	3691.0
90	2545.5
91	1400.0
92	1400.0
93	1400.0
94	859.5
95	319.0
96	319.0
97	319.0
98	732.0
99	1145.0
100	1145.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03709202343809741
2	0.004949833752218302
3	0.0013961069557538804
4	0.003268159464605674
5	0.008186263513284116
6	0.011708260606208677
7	0.01973587560179349
8	0.029825921327469262
9	0.041883208672616405
10	0.049847364261121505
11	0.048800284044306086
12	0.05685962874343076
13	0.0575576822213077
14	0.0595883832478588
15	0.05251265935846982
16	0.05987395057971755
17	0.055971197044314656
18	0.0696466992699947
19	0.067298701208045
20	0.0717725894071654
21	0.06806021409300167
22	0.0706303200797304
23	0.07443788450451372
24	0.0687582675708786
25	0.07142356266822693
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	3151621.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.84057175458976
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.158814584036	44.77318123638416
2	9.427455185299221	10.15159154734564
3	2.77580740822481	4.483531738183491
4	1.269486342029793	2.7339948195810706
5	0.724584845049719	1.9506031171093852
6	0.49447215341431516	1.5973598073929975
7	0.3500571596456385	1.3193094335476216
8	0.2668481762643529	1.1493806705393845
9	0.1883220353017361	0.9125429449170158
>10	1.169294879266465	11.604849528938967
>50	0.0886185025795808	3.3139641321632634
>100	0.07173377658558411	8.132342133737923
>500	0.010183068555741016	3.830695473399
>1k	0.004203475973230068	3.7181104610799176
>5k	1.184077738938047E-4	0.32854295568010966
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCCTAAAGTGTGTATTTCTCATTT	5247	0.16648575447365022	No Hit
GTCCTACAGTGGACATTTCTAAATT	5060	0.16055229991169623	No Hit
CTGTAGGACGTGGAATATGGCAAGA	4771	0.15138241558867643	No Hit
GTATCAACGCAGAGTACTTTTTTTT	4761	0.1510651185532778	No Hit
CTTTAGGACGTGAAATATGGCGAGG	4312	0.13681848166388028	No Hit
GTCCTACAGTGTGCATTTCTCATTT	3373	0.10702429003995087	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.172970353986092E-5
2	0.0	0.0	0.0	0.0	3.172970353986092E-5
3	0.0	0.0	0.0	0.0	3.172970353986092E-5
4	0.0	0.0	0.0	0.0	3.172970353986092E-5
5	0.0	0.0	0.0	0.0	3.172970353986092E-5
6	3.172970353986092E-5	0.0	0.0	0.0	6.345940707972184E-5
7	3.172970353986092E-5	0.0	0.0	0.0	6.345940707972184E-5
8	3.172970353986092E-5	0.0	0.0	3.172970353986092E-5	9.518911061958275E-5
9	3.172970353986092E-5	0.0	0.0	3.172970353986092E-5	9.518911061958275E-5
10	3.172970353986092E-5	0.0	0.0	9.518911061958275E-5	1.903782212391655E-4
11	3.172970353986092E-5	0.0	0.0	9.518911061958275E-5	1.903782212391655E-4
12	3.172970353986092E-5	0.0	0.0	9.518911061958275E-5	2.5383762831888735E-4
13	3.172970353986092E-5	0.0	0.0	9.518911061958275E-5	3.490267389384701E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1065	0.0	14.268461	1
GTATCAA	3315	0.0	13.035708	1
TAGCGTG	60	4.095717E-4	12.666073	5
CCGTCGT	400	0.0	11.875765	9
TTAGACC	105	2.7281203E-7	11.760235	4
ATACCGT	430	0.0	11.709497	6
CGAACGA	245	0.0	11.63414	16
TCCAACG	320	0.0	11.57887	18
TAGGACC	1480	0.0	11.552414	4
TAGAAAT	830	0.0	11.444157	4
CGACCAT	300	0.0	11.400914	10
ACCGTCG	435	0.0	11.138649	8
CGTCGTA	420	0.0	11.084222	10
GTCCTAT	635	0.0	11.067888	1
CCGACCA	320	0.0	10.985082	9
GATATAC	590	0.0	10.946207	1
CTAGGAC	405	0.0	10.787221	3
ACGAACG	265	0.0	10.756092	15
TAACGAA	265	0.0	10.755409	13
GATAACG	275	0.0	10.70995	11
>>END_MODULE