FastQCFastQC Report
Thu 2 Feb 2017
SRR4063880_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063880_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3151621
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA34150.029.3097761
GGTATCA12500.024.2862741
AATTGCG755.4785392E-520.532243
ATTACGC654.9364177E-420.3065723
TCAACGC51850.019.007634
TAGGACC24550.018.8176984
CAACGCA52650.018.802385
TATCAAC53350.018.5553842
CGTCGTA6350.018.36206610
TGTAGGA40400.018.3501822
AACGCAG54150.018.3221666
ACCGTCG6250.018.302998
ATACCGT6900.018.172916
ATCAACG54250.018.1667393
CCGTCGT6450.018.0773839
GTAGGAC41750.017.4409543
TAGGACG41300.017.4179254
CTGTAGG40700.017.2420181
GTCCTAC36550.017.213551
CGCAATA6700.017.07585336
Produced by FastQC (version 0.11.5)