##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063880_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3151621
Sequences flagged as poor quality	0
Sequence length	50
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.215564942612072	32.0	32.0	32.0	32.0	32.0
2	31.00073105236956	32.0	32.0	32.0	32.0	32.0
3	31.27166274117351	32.0	32.0	32.0	32.0	32.0
4	31.434935228569678	32.0	32.0	32.0	32.0	32.0
5	31.301463913332217	32.0	32.0	32.0	32.0	32.0
6	34.94377559992144	36.0	36.0	36.0	36.0	36.0
7	34.9790450691882	36.0	36.0	36.0	36.0	36.0
8	34.88570262731464	36.0	36.0	36.0	32.0	36.0
9	35.083966314477536	36.0	36.0	36.0	36.0	36.0
10	34.846124581604194	36.0	36.0	36.0	32.0	36.0
11	35.09200757324564	36.0	36.0	36.0	36.0	36.0
12	34.96828171915342	36.0	36.0	36.0	36.0	36.0
13	35.03121726882769	36.0	36.0	36.0	36.0	36.0
14	34.96248787528703	36.0	36.0	36.0	32.0	36.0
15	34.899469511086515	36.0	36.0	36.0	32.0	36.0
16	34.936367666035984	36.0	36.0	36.0	32.0	36.0
17	34.90072918031705	36.0	36.0	36.0	32.0	36.0
18	34.90750505850799	36.0	36.0	36.0	32.0	36.0
19	34.908765679629624	36.0	36.0	36.0	32.0	36.0
20	34.89528214211036	36.0	36.0	36.0	32.0	36.0
21	34.89977189516125	36.0	36.0	36.0	32.0	36.0
22	34.87268837211073	36.0	36.0	36.0	32.0	36.0
23	34.82258748751833	36.0	36.0	36.0	32.0	36.0
24	34.8032825012906	36.0	36.0	36.0	32.0	36.0
25	34.76495492319667	36.0	36.0	36.0	32.0	36.0
26	34.70472052318473	36.0	36.0	36.0	32.0	36.0
27	34.68634140970631	36.0	36.0	36.0	32.0	36.0
28	34.655867250535515	36.0	36.0	36.0	32.0	36.0
29	34.62993837139681	36.0	36.0	36.0	32.0	36.0
30	34.60181379677315	36.0	36.0	36.0	32.0	36.0
31	34.60065248962359	36.0	36.0	36.0	32.0	36.0
32	34.55436488080261	36.0	36.0	36.0	32.0	36.0
33	34.54631664149972	36.0	36.0	36.0	32.0	36.0
34	34.548231846405386	36.0	36.0	36.0	32.0	36.0
35	34.50536025746751	36.0	36.0	36.0	32.0	36.0
36	34.46509526367542	36.0	36.0	36.0	32.0	36.0
37	34.443069138072126	36.0	36.0	36.0	32.0	36.0
38	34.39809006222512	36.0	36.0	36.0	32.0	36.0
39	34.36485795722265	36.0	36.0	36.0	32.0	36.0
40	34.381717535198554	36.0	36.0	36.0	32.0	36.0
41	34.36528345254712	36.0	36.0	36.0	32.0	36.0
42	34.30596191610603	36.0	36.0	36.0	32.0	36.0
43	34.30752714238165	36.0	36.0	36.0	32.0	36.0
44	34.232574919382756	36.0	36.0	36.0	32.0	36.0
45	34.20398233163188	36.0	36.0	36.0	32.0	36.0
46	34.17241508417414	36.0	36.0	36.0	32.0	36.0
47	34.158787811097845	36.0	36.0	36.0	32.0	36.0
48	34.110468549359204	36.0	36.0	36.0	32.0	36.0
49	34.09414456877905	36.0	36.0	36.0	32.0	36.0
50	33.422126899141745	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	2.0
18	3.0
19	7.0
20	26.0
21	63.0
22	211.0
23	691.0
24	1829.0
25	4302.0
26	9140.0
27	17700.0
28	31477.0
29	52287.0
30	81817.0
31	126838.0
32	198145.0
33	345079.0
34	716537.0
35	1565467.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.58716569530838	19.115836022325112	12.906589359937984	26.39040892242852
2	15.202190629853678	20.322470614179124	38.85797871243382	25.617360043533377
3	18.197890734219694	24.339942213808907	29.19801828774934	28.264148764222057
4	12.201784415067674	16.682431041042054	36.68493134168099	34.43085320220928
5	14.029066312224725	37.040113643106196	33.7174108181155	15.213409226553573
6	33.74765001435772	36.08292891105037	17.003092065496578	13.166329009095337
7	30.074383991212134	30.65040539481454	20.883044555829876	18.39216605814345
8	27.088568073381918	35.005985808572795	19.105216014235214	18.80023010381007
9	27.08536405820057	14.718408267080935	18.833354909785047	39.36287276493344
10	15.67277127915101	27.108591619637735	32.26075848313526	24.95787861807599
11	36.37160051922487	20.76924858667968	24.03870896913049	18.82044192496496
12	25.29281019413856	23.231993755582454	29.61954426540044	21.855651784878546
13	29.377623458210127	20.142289043737286	25.556437290999877	24.923650207052706
14	22.745988961215122	20.742127448942842	25.425186769323027	31.086696820519006
15	24.08335015084995	29.583344439240534	22.07432326947754	24.258982140431975
16	24.55919966924705	26.148171863344206	25.429805904752417	23.86282256265633
17	23.134031661801973	27.149203536846596	25.97323726425227	23.743527537099162
18	23.80795091780045	25.431850567852443	27.955321897277297	22.804876617069812
19	25.41289876317576	25.0200214492864	25.765574529924294	23.801505257613545
20	25.57334260691216	25.349710591996587	25.68268368571452	23.39426311537673
21	25.553072529977435	24.742727631272924	24.972165117569656	24.73203472117999
22	25.33173415576751	25.82874838971703	25.270495808504833	23.569021646010626
23	23.397617022194737	25.80693063180864	26.20165039822592	24.593801947770704
24	24.811969239868358	25.304098347131564	26.045560176823795	23.83837223617628
25	24.284006290210193	25.47103596817326	26.327816918837758	23.917140822778784
26	23.508304981592786	26.332722192289214	26.574418003974003	23.584554822143993
27	24.175698993925987	26.069171792865397	25.552395113626776	24.202734099581843
28	23.529841063641495	26.058309135961043	25.699751198868608	24.712098601528854
29	24.146726950402993	25.479884285179853	25.47928140550924	24.894107358907913
30	23.6057503753749	25.610150797267096	26.249811201973905	24.534287625384103
31	24.920694103106392	24.96695918169832	25.507479997017203	24.60486671817808
32	24.021307766044842	25.940472666107574	25.422184230057365	24.616035337790223
33	23.423316223955158	25.900876030624264	26.38740810690061	24.288399638519962
34	24.335508035270344	25.68827396611755	26.370035249400054	23.606182749212053
35	24.49444541222558	25.861364876017717	26.044291376371987	23.59989833538471
36	23.602707385335947	25.750052358014585	26.237743464767817	24.40949679188165
37	24.369184744997412	25.687248394979306	25.151093901762227	24.79247295826105
38	24.13216678745908	26.10943919741373	25.495111397684596	24.263282617442595
39	25.036244656907712	25.12759800970903	25.239005311440682	24.597152021942573
40	25.39705002768475	25.224690668574716	25.949761308306535	23.428497995434
41	24.6494633550424	24.95471240113913	26.591919784866057	23.803904458952413
42	24.8670108545796	26.196791253129785	25.79473805620241	23.141459836088202
43	23.760629195714277	25.807285808161563	26.195852935940998	24.236232060183166
44	23.61726674586494	25.866897407779703	25.702186583000803	24.813649263354552
45	23.754370823523438	26.222656572258995	25.73794731531486	24.28502528890271
46	24.122546358331395	25.167288521892594	25.801222303274756	24.908942816501252
47	24.899565516346364	25.191738559702394	25.790744067138878	24.117951856812365
48	24.3619700111944	25.89164913046679	25.218652509093864	24.52772834924495
49	24.471230074054283	25.532022235005826	25.43676924185027	24.559978449089623
50	23.918772145645086	26.536348922238663	25.15364520451494	24.391233727601307
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	15.0
1	23.5
2	32.0
3	86.0
4	140.0
5	186.5
6	233.0
7	232.0
8	231.0
9	301.0
10	371.0
11	614.0
12	857.0
13	1538.0
14	2219.0
15	2866.0
16	3513.0
17	3836.0
18	4159.0
19	4405.0
20	4651.0
21	5770.5
22	6890.0
23	8490.0
24	10090.0
25	14586.0
26	19082.0
27	26897.0
28	34712.0
29	43627.5
30	52543.0
31	63614.5
32	74686.0
33	91974.0
34	109262.0
35	129190.5
36	149119.0
37	159815.5
38	170512.0
39	170838.5
40	171165.0
41	173602.5
42	176040.0
43	174045.0
44	172050.0
45	183578.5
46	195107.0
47	204108.0
48	213109.0
49	217804.5
50	222500.0
51	218187.0
52	213874.0
53	211301.0
54	208728.0
55	206546.0
56	204364.0
57	196691.5
58	189019.0
59	172636.0
60	156253.0
61	137734.5
62	119216.0
63	103397.0
64	87578.0
65	74098.5
66	60619.0
67	50794.5
68	40970.0
69	35985.0
70	31000.0
71	24054.0
72	17108.0
73	14353.5
74	11599.0
75	8953.0
76	6307.0
77	5399.5
78	4492.0
79	3734.0
80	2976.0
81	2397.5
82	1819.0
83	1508.0
84	1197.0
85	888.5
86	580.0
87	408.0
88	236.0
89	188.5
90	141.0
91	120.0
92	99.0
93	77.0
94	55.0
95	54.0
96	53.0
97	40.0
98	27.0
99	25.0
100	23.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.006092103079653296
2	5.076752566377747E-4
3	9.518911061958275E-5
4	0.0
5	0.0
6	1.903782212391655E-4
7	6.345940707972184E-5
8	0.0
9	0.001173999030974854
10	4.7594555309791374E-4
11	0.0
12	1.903782212391655E-4
13	2.5383762831888735E-4
14	1.903782212391655E-4
15	0.004600807013279833
16	7.61512884956662E-4
17	0.0
18	3.1729703539860914E-4
19	3.172970353986092E-5
20	4.7594555309791374E-4
21	0.0
22	3.172970353986092E-5
23	5.711346637174965E-4
24	2.8556733185874824E-4
25	0.0018085931017720724
26	0.0044104287920406675
27	0.005171941676997329
28	0.0034268079823049787
29	0.002538376283188873
30	0.0033316188716853963
31	0.006853615964609957
32	0.0029825921327469264
33	0.004220050570801502
34	0.002474916876109151
35	0.002601835690268595
36	0.007646858553106481
37	0.0032998891681455355
38	0.0066315080398309315
39	0.003871023831863032
40	0.0020941604336308203
41	0.003046051539826648
42	0.001523025769913324
43	0.0013326475486741584
44	0.001173999030974854
45	0.0013009178451342976
46	9.518911061958275E-4
47	0.0020307010265510988
48	0.0014278366592937412
49	4.442158495580528E-4
50	0.0013326475486741584
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	3151621.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.40467369725958
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.07342884561085	51.38832709726153
2	8.681596814955677	10.488180455571312
3	2.3908904483072386	4.332628721276802
4	1.0809427211252818	2.61175969420002
5	0.6127526774266325	1.8506562768538946
6	0.41389576835943775	1.5000743299457024
7	0.27800321291338514	1.1754885353975966
8	0.2200632011560735	1.0634276686885613
9	0.1732358730859508	0.9417830747775058
>10	0.9467294042375918	10.356336199122417
>50	0.06804035659831772	2.8369229731454766
>100	0.05143513448179771	6.5194021622064335
>500	0.006395591475499421	2.6538197499827216
>1k	0.0025899502662490004	2.2811930615700984
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	3.172970353986092E-5	6.345940707972184E-5
2	0.0	0.0	0.0	3.172970353986092E-5	6.345940707972184E-5
3	0.0	0.0	0.0	3.172970353986092E-5	6.345940707972184E-5
4	0.0	0.0	0.0	3.172970353986092E-5	6.345940707972184E-5
5	0.0	0.0	0.0	3.172970353986092E-5	9.518911061958275E-5
6	0.0	0.0	0.0	3.172970353986092E-5	9.518911061958275E-5
7	3.172970353986092E-5	3.172970353986092E-5	0.0	6.345940707972184E-5	9.518911061958275E-5
8	3.172970353986092E-5	3.172970353986092E-5	0.0	9.518911061958275E-5	9.518911061958275E-5
9	3.172970353986092E-5	3.172970353986092E-5	0.0	9.518911061958275E-5	9.518911061958275E-5
10	3.172970353986092E-5	3.172970353986092E-5	0.0	9.518911061958275E-5	9.518911061958275E-5
11	3.172970353986092E-5	3.172970353986092E-5	0.0	9.518911061958275E-5	9.518911061958275E-5
12	3.172970353986092E-5	3.172970353986092E-5	0.0	9.518911061958275E-5	3.1729703539860914E-4
13	3.172970353986092E-5	3.172970353986092E-5	0.0	9.518911061958275E-5	3.490267389384701E-4
14	3.172970353986092E-5	3.172970353986092E-5	0.0	9.518911061958275E-5	3.490267389384701E-4
15	3.172970353986092E-5	3.172970353986092E-5	0.0	1.2691881415944367E-4	4.7594555309791374E-4
16	6.345940707972184E-5	3.172970353986092E-5	0.0	1.2691881415944367E-4	5.394049601776356E-4
17	6.345940707972184E-5	3.172970353986092E-5	0.0	1.2691881415944367E-4	5.394049601776356E-4
18	6.345940707972184E-5	3.172970353986092E-5	0.0	1.2691881415944367E-4	5.394049601776356E-4
19	6.345940707972184E-5	3.172970353986092E-5	0.0	1.2691881415944367E-4	5.394049601776356E-4
20	6.345940707972184E-5	3.172970353986092E-5	0.0	1.2691881415944367E-4	6.345940707972183E-4
21	6.345940707972184E-5	3.172970353986092E-5	0.0	1.5864851769930457E-4	6.663237743370793E-4
22	6.345940707972184E-5	3.172970353986092E-5	0.0	2.2210792477902642E-4	6.663237743370793E-4
23	6.345940707972184E-5	3.172970353986092E-5	0.0	2.5383762831888735E-4	6.663237743370793E-4
24	6.345940707972184E-5	3.172970353986092E-5	0.0	3.1729703539860914E-4	6.980534778769402E-4
25	6.345940707972184E-5	3.172970353986092E-5	0.0	3.80756442478331E-4	6.980534778769402E-4
26	6.345940707972184E-5	3.172970353986092E-5	0.0	4.124861460181919E-4	7.61512884956662E-4
27	6.345940707972184E-5	3.172970353986092E-5	0.0	5.076752566377747E-4	7.932425884965229E-4
28	6.345940707972184E-5	3.172970353986092E-5	0.0	9.836208097356884E-4	7.932425884965229E-4
29	6.345940707972184E-5	3.172970353986092E-5	0.0	0.0025066465796490123	7.932425884965229E-4
30	6.345940707972184E-5	3.172970353986092E-5	0.0	0.005711346637174965	7.932425884965229E-4
31	6.345940707972184E-5	3.172970353986092E-5	0.0	0.015737932955771014	8.249722920363838E-4
32	6.345940707972184E-5	3.172970353986092E-5	0.0	0.02697024800888178	9.201614026559666E-4
33	6.345940707972184E-5	3.172970353986092E-5	0.0	0.039027535354028926	9.518911061958275E-4
34	6.345940707972184E-5	3.172970353986092E-5	0.0	0.05394049601776356	0.0010788099203552712
35	6.345940707972184E-5	3.172970353986092E-5	0.0	0.07034475274787165	0.0010788099203552712
36	6.345940707972184E-5	3.172970353986092E-5	0.0	0.09366608484966943	0.0010788099203552712
37	6.345940707972184E-5	3.172970353986092E-5	0.0	0.13158308057980322	0.0010788099203552712
38	6.345940707972184E-5	3.172970353986092E-5	0.0	0.18476206371261011	0.001110539623895132
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	3415	0.0	29.309776	1
GGTATCA	1250	0.0	24.286274	1
AATTGCG	75	5.4785392E-5	20.5322	43
ATTACGC	65	4.9364177E-4	20.306572	3
TCAACGC	5185	0.0	19.00763	4
TAGGACC	2455	0.0	18.817698	4
CAACGCA	5265	0.0	18.80238	5
TATCAAC	5335	0.0	18.555384	2
CGTCGTA	635	0.0	18.362066	10
TGTAGGA	4040	0.0	18.350182	2
AACGCAG	5415	0.0	18.322166	6
ACCGTCG	625	0.0	18.30299	8
ATACCGT	690	0.0	18.17291	6
ATCAACG	5425	0.0	18.166739	3
CCGTCGT	645	0.0	18.077383	9
GTAGGAC	4175	0.0	17.440954	3
TAGGACG	4130	0.0	17.417925	4
CTGTAGG	4070	0.0	17.242018	1
GTCCTAC	3655	0.0	17.21355	1
CGCAATA	670	0.0	17.075853	36
>>END_MODULE