FastQCFastQC Report
Thu 2 Feb 2017
SRR4063879_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063879_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2746974
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT38860.1414647535797572No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8950.014.2215411
AGTATCG707.2611365E-613.572568
GTATCAA26950.013.14664651
CGTCGTA2350.012.53296110
CGCATCG2400.011.87620713
CCGTCGT2350.011.7243829
TAGTATC907.46389E-611.6114447
TATACCG907.474495E-611.6099645
CGTCTTA1354.7293724E-911.25999415
AGAACCG2400.011.0822395
AACCGCG1408.356437E-910.8574557
ACCGTCG2750.010.7099858
GTTCTAG1701.4006218E-1010.6162121
CGACCAT2150.010.60553610
TCGGTTG909.510449E-510.55662817
GTCCTAA4950.010.5541291
GTCGTAG2800.010.51835211
TAAGACC2100.010.4031664
GTACTAT1201.5274727E-610.2902761
TATGCCG750.002650847210.1323335
Produced by FastQC (version 0.11.5)