##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063879_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2746974
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.28552217822229	32.0	32.0	32.0	32.0	32.0
2	30.8382048756195	32.0	32.0	32.0	32.0	32.0
3	30.85639325308503	32.0	32.0	32.0	32.0	32.0
4	30.888546451477154	32.0	32.0	32.0	32.0	32.0
5	30.778722332282722	32.0	32.0	32.0	32.0	32.0
6	34.46977656140903	36.0	36.0	36.0	32.0	36.0
7	34.412486612541656	36.0	36.0	36.0	32.0	36.0
8	34.36443300883081	36.0	36.0	36.0	32.0	36.0
9	34.533531442234256	36.0	36.0	36.0	32.0	36.0
10	34.18460313057204	36.0	36.0	36.0	32.0	36.0
11	34.504541724821564	36.0	36.0	36.0	32.0	36.0
12	34.303132465032434	36.0	36.0	36.0	32.0	36.0
13	34.38835933649172	36.0	36.0	36.0	32.0	36.0
14	34.28402635044962	36.0	36.0	36.0	32.0	36.0
15	34.21324446463636	36.0	36.0	36.0	32.0	36.0
16	34.210519284128644	36.0	36.0	36.0	32.0	36.0
17	34.143019191299224	36.0	36.0	36.0	32.0	36.0
18	34.138191333445455	36.0	36.0	36.0	32.0	36.0
19	34.15468948741415	36.0	36.0	36.0	32.0	36.0
20	34.13828525497511	36.0	36.0	36.0	32.0	36.0
21	34.1290540063357	36.0	36.0	36.0	32.0	36.0
22	34.10811423770301	36.0	36.0	36.0	32.0	36.0
23	34.04890035362548	36.0	36.0	36.0	32.0	36.0
24	34.03235815118745	36.0	36.0	36.0	32.0	36.0
25	33.61899748596092	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	6.0
4	26.0
5	34.0
6	112.0
7	45.0
8	153.0
9	183.0
10	248.0
11	85.0
12	150.0
13	143.0
14	241.0
15	377.0
16	733.0
17	938.0
18	1250.0
19	1658.0
20	2575.0
21	3904.0
22	6147.0
23	9673.0
24	14403.0
25	21175.0
26	30395.0
27	40429.0
28	54655.0
29	73746.0
30	96648.0
31	132326.0
32	187715.0
33	267674.0
34	572613.0
35	1226514.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.791003248503216	16.545151281192187	11.014465308025114	26.649380162279492
2	16.874105334282792	19.24101038431126	37.512505297003536	26.372378984402417
3	19.2647989276725	23.057880784383393	28.27943421764433	29.397886070299776
4	13.06189137346229	15.246639709836064	35.22642754766259	36.46504136903906
5	14.872595452835979	36.16360295523065	33.6398683085262	15.323933283407174
6	34.75670007755014	34.57068895838871	16.890057197781992	13.78255376627916
7	30.737350797910977	30.01166271416082	20.443940504500684	18.80704598342751
8	28.096333031558203	33.308108525426675	19.092496869963078	19.503061573052037
9	27.600485276533686	14.32844159727511	18.03299108298906	40.03808204320214
10	15.893520388631712	26.654323717221402	31.854316286683275	25.597839607463612
11	37.0635748544521	20.707794024231756	22.7691397667737	19.459491354542436
12	25.03934053605123	23.056067549157458	28.917588862257126	22.987003052534185
13	29.88466933820286	19.495293120411826	24.788169473163883	25.831868068221432
14	23.551592180348145	19.338211053372603	24.953854728455976	32.15634203782328
15	25.25167091566798	27.15343138272665	21.864854439377698	25.73004326222767
16	25.743018965798615	25.455639614855567	23.648745133238524	25.152596286107293
17	24.273718658092452	25.70883325088898	25.05735285868212	24.96009523233645
18	24.845284844563505	24.904558773543354	25.83993561838519	24.410220763507954
19	25.893513861864783	24.605233486473658	24.938933914564675	24.562318737096884
20	25.987681487839602	24.46389770956991	24.686754502676322	24.861666299914166
21	26.32338229797441	24.15622151612365	24.28707885757752	25.23331732832442
22	26.039134679840288	24.439319751683133	24.43793535600828	25.083610212468304
23	24.85401987741568	24.398199745475228	24.979715621576386	25.76806475553271
24	25.216903756483433	24.52255671557346	24.954342282962358	25.306197244980748
25	25.146500936476613	24.3392111008819	25.09972264553823	25.41456531710326
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	87.0
1	87.0
2	281.0
3	475.0
4	475.0
5	475.0
6	1335.0
7	2195.0
8	2195.0
9	2195.0
10	2651.5
11	3108.0
12	3108.0
13	3108.0
14	4425.5
15	5743.0
16	5743.0
17	5743.0
18	9894.5
19	14046.0
20	14046.0
21	14046.0
22	23340.5
23	32635.0
24	32635.0
25	32635.0
26	50250.5
27	67866.0
28	67866.0
29	67866.0
30	89401.0
31	110936.0
32	110936.0
33	110936.0
34	137510.5
35	164085.0
36	164085.0
37	164085.0
38	192201.5
39	220318.0
40	220318.0
41	220318.0
42	253884.0
43	287450.0
44	287450.0
45	287450.0
46	321402.0
47	355354.0
48	355354.0
49	355354.0
50	367115.5
51	378877.0
52	378877.0
53	378877.0
54	361289.0
55	343701.0
56	343701.0
57	343701.0
58	315359.0
59	287017.0
60	287017.0
61	287017.0
62	249191.5
63	211366.0
64	211366.0
65	211366.0
66	171469.0
67	131572.0
68	131572.0
69	131572.0
70	99407.5
71	67243.0
72	67243.0
73	67243.0
74	50141.5
75	33040.0
76	33040.0
77	33040.0
78	25098.5
79	17157.0
80	17157.0
81	17157.0
82	11802.0
83	6447.0
84	6447.0
85	6447.0
86	4759.5
87	3072.0
88	3072.0
89	3072.0
90	2216.5
91	1361.0
92	1361.0
93	1361.0
94	952.5
95	544.0
96	544.0
97	544.0
98	911.5
99	1279.0
100	1279.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03982564086882511
2	0.005023709725683607
3	0.0018929920705474462
4	0.004113617384074258
5	0.009137327109757863
6	0.013250944493832122
7	0.02238827160358999
8	0.03640369366437396
9	0.048162086717966755
10	0.05631651409878652
11	0.05409588878525971
12	0.06239593094073697
13	0.06323321589501757
14	0.06694639264878371
15	0.059083194817278945
16	0.06632752985648936
17	0.06144943490546324
18	0.07612012345220595
19	0.0726981762477548
20	0.0787047857023765
21	0.07280738732874792
22	0.07622933453319908
23	0.08194471443850579
24	0.07619293083953471
25	0.07684819732549343
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2746974.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.33330082198775
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.11001091099273	44.327513325906814
2	11.125213103100993	12.311895266852142
3	3.3730777583173372	5.5993057889078806
4	1.5344152717531145	3.396170472710687
5	0.8655294697428296	2.394630125978777
6	0.5602218965263018	1.859935603653261
7	0.4076738809887902	1.5790559045813977
8	0.29267655862205916	1.2955808049422826
9	0.22481949978419036	1.1196004510986675
>10	1.3551324452299915	13.793832174362915
>50	0.08858363494729322	3.3768475014958694
>100	0.05636838982747355	6.053374841243481
>500	0.004757441810824089	1.748641201030831
>1k	0.0015197383562107745	1.1436165372351248
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3886	0.1414647535797572	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	7.280738732874793E-5
2	0.0	0.0	0.0	0.0	7.280738732874793E-5
3	0.0	0.0	0.0	0.0	7.280738732874793E-5
4	0.0	0.0	0.0	0.0	7.280738732874793E-5
5	0.0	0.0	0.0	3.6403693664373964E-5	7.280738732874793E-5
6	0.0	0.0	0.0	3.6403693664373964E-5	1.4561477465749586E-4
7	0.0	0.0	0.0	3.6403693664373964E-5	1.820184683218698E-4
8	0.0	0.0	0.0	3.6403693664373964E-5	1.820184683218698E-4
9	0.0	0.0	0.0	3.6403693664373964E-5	2.1842216198624377E-4
10	0.0	0.0	0.0	3.6403693664373964E-5	2.1842216198624377E-4
11	0.0	0.0	0.0	3.6403693664373964E-5	2.1842216198624377E-4
12	0.0	0.0	0.0	3.6403693664373964E-5	4.732480176368615E-4
13	0.0	0.0	0.0	3.6403693664373964E-5	5.824590986299834E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	895	0.0	14.221541	1
AGTATCG	70	7.2611365E-6	13.57256	8
GTATCAA	2695	0.0	13.1466465	1
CGTCGTA	235	0.0	12.532961	10
CGCATCG	240	0.0	11.876207	13
CCGTCGT	235	0.0	11.724382	9
TAGTATC	90	7.46389E-6	11.611444	7
TATACCG	90	7.474495E-6	11.609964	5
CGTCTTA	135	4.7293724E-9	11.259994	15
AGAACCG	240	0.0	11.082239	5
AACCGCG	140	8.356437E-9	10.857455	7
ACCGTCG	275	0.0	10.709985	8
GTTCTAG	170	1.4006218E-10	10.616212	1
CGACCAT	215	0.0	10.605536	10
TCGGTTG	90	9.510449E-5	10.556628	17
GTCCTAA	495	0.0	10.554129	1
GTCGTAG	280	0.0	10.518352	11
TAAGACC	210	0.0	10.403166	4
GTACTAT	120	1.5274727E-6	10.290276	1
TATGCCG	75	0.0026508472	10.132333	5
>>END_MODULE