##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063879_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2746974
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.361760613678907	32.0	32.0	32.0	32.0	32.0
2	31.44766459384035	32.0	32.0	32.0	32.0	32.0
3	31.51107873609288	32.0	32.0	32.0	32.0	32.0
4	31.585920361823593	32.0	32.0	32.0	32.0	32.0
5	31.55266012710714	32.0	32.0	32.0	32.0	32.0
6	35.15453185942059	36.0	36.0	36.0	36.0	36.0
7	35.168487215386826	36.0	36.0	36.0	36.0	36.0
8	35.11689153228243	36.0	36.0	36.0	36.0	36.0
9	35.21002819830111	36.0	36.0	36.0	36.0	36.0
10	35.08398441339452	36.0	36.0	36.0	36.0	36.0
11	35.21311159115449	36.0	36.0	36.0	36.0	36.0
12	35.133313602531366	36.0	36.0	36.0	36.0	36.0
13	35.16806129217095	36.0	36.0	36.0	36.0	36.0
14	35.109210716956184	36.0	36.0	36.0	36.0	36.0
15	35.084678267795766	36.0	36.0	36.0	36.0	36.0
16	35.10266824513082	36.0	36.0	36.0	36.0	36.0
17	35.07167049997561	36.0	36.0	36.0	36.0	36.0
18	35.06985067932933	36.0	36.0	36.0	36.0	36.0
19	35.06077451042493	36.0	36.0	36.0	36.0	36.0
20	35.05797324619745	36.0	36.0	36.0	36.0	36.0
21	35.05029789142525	36.0	36.0	36.0	36.0	36.0
22	35.03624060511676	36.0	36.0	36.0	36.0	36.0
23	34.98761400726763	36.0	36.0	36.0	36.0	36.0
24	34.963228629029615	36.0	36.0	36.0	36.0	36.0
25	34.93960044761982	36.0	36.0	36.0	36.0	36.0
26	34.88267235146747	36.0	36.0	36.0	32.0	36.0
27	34.870883379311195	36.0	36.0	36.0	32.0	36.0
28	34.851841699266174	36.0	36.0	36.0	32.0	36.0
29	34.825495618087395	36.0	36.0	36.0	32.0	36.0
30	34.81199530829196	36.0	36.0	36.0	32.0	36.0
31	34.80469818789694	36.0	36.0	36.0	32.0	36.0
32	34.774419051654654	36.0	36.0	36.0	32.0	36.0
33	34.75893000807434	36.0	36.0	36.0	32.0	36.0
34	34.75600751954696	36.0	36.0	36.0	32.0	36.0
35	34.72335886688407	36.0	36.0	36.0	32.0	36.0
36	34.699902510908366	36.0	36.0	36.0	32.0	36.0
37	34.68444623065235	36.0	36.0	36.0	32.0	36.0
38	34.65816458401135	36.0	36.0	36.0	32.0	36.0
39	34.64264860169772	36.0	36.0	36.0	32.0	36.0
40	34.63778506822416	36.0	36.0	36.0	32.0	36.0
41	34.625441667813384	36.0	36.0	36.0	32.0	36.0
42	34.5778973517769	36.0	36.0	36.0	32.0	36.0
43	34.57035487048658	36.0	36.0	36.0	32.0	36.0
44	34.524725024699904	36.0	36.0	36.0	32.0	36.0
45	34.49605820805002	36.0	36.0	36.0	32.0	36.0
46	34.47788512013583	36.0	36.0	36.0	32.0	36.0
47	34.454372702471886	36.0	36.0	36.0	32.0	36.0
48	34.42203857772225	36.0	36.0	36.0	32.0	36.0
49	34.40549965161665	36.0	36.0	36.0	32.0	36.0
50	33.83196819482092	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	2.0
18	3.0
19	10.0
20	24.0
21	72.0
22	258.0
23	858.0
24	1994.0
25	4610.0
26	9487.0
27	16979.0
28	28453.0
29	43765.0
30	63228.0
31	90144.0
32	129960.0
33	211171.0
34	450078.0
35	1695877.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.94038962348677	17.35975822649919	11.523961636915239	27.175890513098793
2	16.16298884476336	19.91244142162921	37.04264458068389	26.881925152923543
3	18.57562124904349	23.57448181011341	28.474287632245904	29.375609308597195
4	12.729315967315308	15.850022606693765	35.280930944377346	36.13973048161358
5	14.496860909495322	36.52564603814962	33.48415383618483	15.493339216170229
6	34.8993271488154	34.86827471899007	16.627684278697195	13.60471385349733
7	30.732152153099594	30.101457241979347	20.444817380604814	18.721573224316245
8	28.056654340375992	33.431332076677826	19.001271945056633	19.51074163788955
9	27.58299328816548	14.148876258264199	17.967854400843994	40.300276052726325
10	15.790075309061297	26.786283099018654	31.969710373006833	25.45393121891322
11	37.289031494291535	20.664447497500888	22.693443767578433	19.353077240629144
12	25.063961522611873	23.017265606684326	28.86706924444587	23.051703626257936
13	29.938160707895534	19.37473402406079	24.831750310070156	25.855354957973525
14	23.611667280677867	19.264176742032294	24.910555799056706	32.21360017823313
15	25.25468347093989	27.22025046225828	21.753279494517518	25.771786572284306
16	25.741876814738664	25.464514851369806	23.65148717560409	25.142121158287438
17	24.3595680192095	25.737447824406058	24.978576426278515	24.924407730105926
18	24.991699915543002	24.851253749599557	25.831683024143054	24.32536331071439
19	25.93815083002272	24.55895998977784	24.93901468998785	24.56387449021159
20	26.07338892460522	24.465644492044643	24.65698369903537	24.80398288431477
21	26.358167205077297	24.167429324048936	24.269032033066203	25.205371437807568
22	26.07565712355277	24.37775121115177	24.44535291950555	25.10123874578991
23	24.9420811751806	24.329000767397126	24.95831737622991	25.770600681192363
24	25.29079418702857	24.533375076448145	24.878665870635174	25.29716486588811
25	25.264940935945653	24.255342230771554	25.123401262426132	25.356315570856662
26	24.9627388119961	24.785807328167127	25.22711667939651	25.02433718044026
27	25.304689249184964	24.87579250877835	24.564195258872548	25.255322983164135
28	25.05805700963533	24.623525912593575	24.87537629206198	25.44304078570911
29	25.134078220425614	24.692490163471312	24.708581006107238	25.464850609995835
30	25.02496485988916	24.737618685474075	24.98164268231993	25.255773772316836
31	25.49297441153074	24.345798390458526	24.594310720507366	25.56691647750337
32	25.294342714997324	24.76264915542453	24.443378185383214	25.49962994419493
33	24.913246018709444	24.615632171856618	24.915976420974147	25.555145388459792
34	25.360671391766182	24.50341131118781	25.149596398255337	24.98632089879067
35	25.58375150351962	24.486728253936818	25.056209062764843	24.873311179778717
36	25.087757620769118	24.684811615097004	24.880750845538575	25.346679918595292
37	25.599575078762605	24.461804051313827	24.56046208364308	25.37815878628049
38	25.16368423505289	24.76557767137332	24.7433332168321	25.327404876741692
39	25.57717742337703	24.44191711332835	24.535078065528666	25.445827397765957
40	25.82400800317739	24.439579177905056	24.858559202361636	24.877853616555917
41	25.21099415809299	24.463381505615285	25.209101099175534	25.11652323711619
42	25.693312621658944	24.797036390926422	24.824994967092245	24.68465602032239
43	25.148065672026483	24.361585672405088	25.06480887888011	25.425539776688318
44	24.883779515634522	24.635102263104976	24.889895425226815	25.591222796033687
45	24.911128156383867	24.771153865056046	24.965516086486275	25.35220189207382
46	25.040718005695062	24.361417168618775	25.014689061509127	25.583175764177035
47	25.313062225099003	24.450161600467148	25.085241607078242	25.151534567355615
48	25.279959110552586	24.89236122361089	24.61233840531883	25.215341260517693
49	25.118785857821006	24.598938462882604	24.76978186795585	25.512493811340537
50	24.89866022169339	25.08166338848183	24.723920774590844	25.29575561523393
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	26.0
1	33.0
2	40.0
3	175.5
4	311.0
5	331.0
6	351.0
7	335.5
8	320.0
9	408.5
10	497.0
11	708.0
12	919.0
13	1725.0
14	2531.0
15	3184.0
16	3837.0
17	3846.0
18	3855.0
19	3779.0
20	3703.0
21	4325.0
22	4947.0
23	6035.5
24	7124.0
25	9594.5
26	12065.0
27	16103.0
28	20141.0
29	24757.5
30	29374.0
31	35020.0
32	40666.0
33	49004.0
34	57342.0
35	67834.5
36	78327.0
37	89297.5
38	100268.0
39	108045.5
40	115823.0
41	125693.0
42	135563.0
43	141856.0
44	148149.0
45	161709.5
46	175270.0
47	185824.5
48	196379.0
49	204032.5
50	211686.0
51	213081.0
52	214476.0
53	214091.0
54	213706.0
55	211170.0
56	208634.0
57	201928.5
58	195223.0
59	179310.0
60	163397.0
61	144083.5
62	124770.0
63	108789.0
64	92808.0
65	78377.5
66	63947.0
67	53594.0
68	43241.0
69	36892.0
70	30543.0
71	24013.0
72	17483.0
73	14520.5
74	11558.0
75	9034.0
76	6510.0
77	5517.0
78	4524.0
79	3780.0
80	3036.0
81	2389.5
82	1743.0
83	1371.0
84	999.0
85	720.0
86	441.0
87	302.5
88	164.0
89	128.0
90	92.0
91	80.5
92	69.0
93	50.0
94	31.0
95	28.5
96	26.0
97	26.0
98	26.0
99	19.5
100	13.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009865400983045343
2	4.7324801763686154E-4
3	2.912295493149917E-4
4	0.0
5	0.0
6	2.548258556506177E-4
7	1.4561477465749586E-4
8	0.0
9	0.0012741292782530887
10	9.82899728938097E-4
11	0.0
12	3.6403693664373966E-4
13	5.460554049656094E-4
14	3.6403693664373966E-4
15	0.0048416912573617365
16	8.008812606162271E-4
17	0.0
18	5.096517113012354E-4
19	3.6403693664373964E-5
20	7.280738732874793E-4
21	0.0
22	7.280738732874793E-5
23	9.464960352737231E-4
24	5.096517113012354E-4
25	0.0014925514402393326
26	0.005387746662327346
27	0.0057881872926354595
28	0.003822387834759266
29	0.0025482585565061774
30	0.004113617384074258
31	0.008336445849141639
32	0.0033127361234580304
33	0.004586865401711119
34	0.003021506574143039
35	0.003130717655136161
36	0.007426353507532288
37	0.0040772136904098834
38	0.00808161999349102
39	0.004186424771403006
40	0.0023662400881843074
41	0.0035311582854442745
42	0.00192939576421182
43	0.001856588376883072
44	0.0014561477465749586
45	0.0014925514402393326
46	0.0011649181972599669
47	0.0027666807184924215
48	0.002002203151540568
49	5.096517113012354E-4
50	9.82899728938097E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2746974.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.27480064659162
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.73553796442425	48.448577185831695
2	10.472393289688684	12.414980490780012
3	3.055401842523234	5.433250053323803
4	1.3202974836363406	3.1304148054696266
5	0.7999838449405607	2.3709441464672802
6	0.509086701025496	1.8105607629070357
7	0.3418383460587533	1.418367987119524
8	0.27446828417768104	1.301524226275528
9	0.19299998951031191	1.0296032312716212
>10	1.176242702608608	12.75626510741833
>50	0.07501076580298273	3.0588951779196556
>100	0.0422866372113888	4.8669378612729775
>500	0.0036482882654243383	1.4003937862544895
>1k	8.038601262555386E-4	0.5592851776881419
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.6403693664373964E-5
2	0.0	0.0	0.0	0.0	3.6403693664373964E-5
3	0.0	0.0	0.0	0.0	3.6403693664373964E-5
4	0.0	0.0	0.0	0.0	7.280738732874793E-5
5	0.0	0.0	0.0	3.6403693664373964E-5	7.280738732874793E-5
6	0.0	0.0	0.0	3.6403693664373964E-5	2.1842216198624377E-4
7	0.0	0.0	0.0	3.6403693664373964E-5	2.1842216198624377E-4
8	0.0	0.0	0.0	3.6403693664373964E-5	2.548258556506177E-4
9	0.0	0.0	0.0	1.0921108099312189E-4	2.548258556506177E-4
10	0.0	0.0	0.0	1.0921108099312189E-4	2.912295493149917E-4
11	0.0	0.0	0.0	1.0921108099312189E-4	2.912295493149917E-4
12	0.0	0.0	0.0	1.0921108099312189E-4	4.004406303081136E-4
13	0.0	0.0	0.0	1.4561477465749586E-4	5.460554049656095E-4
14	0.0	0.0	0.0	1.4561477465749586E-4	5.460554049656095E-4
15	0.0	0.0	0.0	1.4561477465749586E-4	6.188627922943574E-4
16	0.0	0.0	0.0	1.4561477465749586E-4	6.188627922943574E-4
17	0.0	0.0	0.0	1.820184683218698E-4	6.188627922943574E-4
18	0.0	0.0	0.0	1.820184683218698E-4	6.916701796231053E-4
19	0.0	0.0	0.0	2.548258556506177E-4	7.280738732874792E-4
20	0.0	0.0	0.0	2.912295493149917E-4	7.644775669518533E-4
21	0.0	0.0	0.0	2.912295493149917E-4	8.008812606162272E-4
22	0.0	0.0	0.0	3.2763324297936566E-4	8.008812606162272E-4
23	0.0	0.0	0.0	5.096517113012354E-4	8.008812606162272E-4
24	0.0	0.0	0.0	6.188627922943574E-4	8.008812606162272E-4
25	0.0	0.0	0.0	9.10092341609349E-4	8.008812606162272E-4
26	0.0	0.0	0.0	0.0010557071162668448	8.008812606162272E-4
27	0.0	0.0	0.0	0.0014561477465749584	8.372849542806011E-4
28	0.0	0.0	0.0	0.002803084412156795	8.372849542806011E-4
29	0.0	0.0	0.0	0.005933802067292956	9.10092341609349E-4
30	0.0	0.0	0.0	0.012559274314209017	9.10092341609349E-4
31	0.0	0.0	0.0	0.03538439024177149	9.46496035273723E-4
32	0.0	0.0	0.0	0.05817310247566959	0.0010193034226024709
33	0.0	0.0	0.0	0.0827819993927864	0.0010557071162668448
34	0.0	0.0	0.0	0.1112860915319912	0.0012013218909243408
35	0.0	0.0	0.0	0.14171957943540783	0.0012013218909243408
36	0.0	0.0	0.0	0.18620489309327282	0.0012013218909243408
37	0.0	0.0	0.0	0.25915789519667826	0.0012013218909243408
38	0.0	0.0	0.0	0.3541351319670299	0.0012377255845887147
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	2780	0.0	28.09191	1
GGTATCA	1200	0.0	24.931898	1
TCAACGC	4065	0.0	18.723606	4
TATCAAC	4275	0.0	18.318747	2
CAACGCA	4205	0.0	18.10023	5
ATCAACG	4305	0.0	17.679783	3
AACGCAG	4355	0.0	17.577822	6
GTAGGAC	1445	0.0	16.745527	3
GTATAAC	315	0.0	16.062569	1
GTCCTAC	1375	0.0	15.519107	1
TGTAGGA	1535	0.0	15.3340645	2
GTATAAT	260	1.8189894E-12	15.229895	1
TCTATAC	290	0.0	15.170713	3
TAGGACG	1075	0.0	15.1424885	4
ACGCAGA	5070	0.0	15.01185	7
TCCTACA	1425	0.0	14.819661	2
TAGGACC	1070	0.0	14.802077	4
TAAGACT	450	0.0	14.665023	4
TAGGACA	675	0.0	14.665022	4
ACCGTCG	360	0.0	14.664756	8
>>END_MODULE