FastQCFastQC Report
Thu 2 Feb 2017
SRR4063878_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063878_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7423169
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT99400.1339050747733212No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA33400.015.4949811
GTATCAA122800.013.7027061
CGCGTAA1207.403287E-1012.66808710
TCGCGTA1251.4115358E-912.16111859
GCGGTAA1303.2627213E-810.96395115
ACCGCGC1106.048338E-610.36444958
TAGGACC11950.09.9342674
ACCGTAC1356.4469714E-79.8526258
GCACCGT3400.09.77839856
GTATTAG6450.09.5696191
GTAGGAC13450.09.5311793
TATCAAC178450.09.38565352
GACCGCG2850.09.3333737
GCGATTC2106.675691E-109.04863513
CCTATAC7150.09.0310453
GTCGCGT2002.6593625E-99.0257088
ATCAACG185900.08.96974853
TGCGATT2553.6379788E-128.9424212
TCAACGC187350.08.8711124
GCGTAAC1502.7013866E-68.86772111
Produced by FastQC (version 0.11.5)