##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063878_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7423169
Sequences flagged as poor quality	0
Sequence length	25
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.30945314595424	32.0	32.0	32.0	32.0	32.0
2	30.963347863964838	32.0	32.0	32.0	32.0	32.0
3	30.972674338951464	32.0	32.0	32.0	32.0	32.0
4	31.000253934673992	32.0	32.0	32.0	32.0	32.0
5	30.937968002614518	32.0	32.0	32.0	32.0	32.0
6	34.59347645190349	36.0	36.0	36.0	32.0	36.0
7	34.53856755787184	36.0	36.0	36.0	32.0	36.0
8	34.51619072662902	36.0	36.0	36.0	32.0	36.0
9	34.63127513330223	36.0	36.0	36.0	32.0	36.0
10	34.38544926028223	36.0	36.0	36.0	32.0	36.0
11	34.607306259631166	36.0	36.0	36.0	32.0	36.0
12	34.46373819591067	36.0	36.0	36.0	32.0	36.0
13	34.507714697051895	36.0	36.0	36.0	32.0	36.0
14	34.42399371481371	36.0	36.0	36.0	32.0	36.0
15	34.38259266359152	36.0	36.0	36.0	32.0	36.0
16	34.37593068405152	36.0	36.0	36.0	32.0	36.0
17	34.32633124747665	36.0	36.0	36.0	32.0	36.0
18	34.31009613279719	36.0	36.0	36.0	32.0	36.0
19	34.314446565880424	36.0	36.0	36.0	32.0	36.0
20	34.295512738562195	36.0	36.0	36.0	32.0	36.0
21	34.28616241931175	36.0	36.0	36.0	32.0	36.0
22	34.26208995107076	36.0	36.0	36.0	32.0	36.0
23	34.204267341885924	36.0	36.0	36.0	32.0	36.0
24	34.20297894335963	36.0	36.0	36.0	32.0	36.0
25	33.7956480581272	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	24.0
4	54.0
5	84.0
6	300.0
7	114.0
8	390.0
9	504.0
10	673.0
11	198.0
12	477.0
13	435.0
14	625.0
15	1097.0
16	1864.0
17	2421.0
18	3122.0
19	4051.0
20	6014.0
21	9109.0
22	14135.0
23	22150.0
24	33385.0
25	49789.0
26	71527.0
27	97606.0
28	133842.0
29	182213.0
30	241949.0
31	329476.0
32	468584.0
33	670851.0
34	1491929.0
35	3584176.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.875668689721905	17.362449875645105	12.13368201668532	25.628199417947666
2	15.850700443700788	20.033801283748236	38.854582404606354	25.26091586794462
3	18.589516991644956	24.410849434060886	28.336979628347343	28.66265394594682
4	12.470710527935957	16.498172072895066	36.02732036003772	35.003797039131264
5	14.145039354760309	37.25177103831306	33.77137189419927	14.83181771272736
6	32.699522590648364	35.9702899782839	18.058937702889544	13.2712497281782
7	30.507593030336146	30.77815911846119	20.5232866719118	18.19096117929086
8	27.996261697356505	33.58750377334081	19.208231812497303	19.20800271680538
9	27.937586174828255	15.139342085932899	18.480910464437677	38.442161274801165
10	15.367147427926586	28.57454225777845	32.35155163347895	23.706758680816012
11	35.474067121026934	22.351651337713754	22.697422158770173	19.47685938248914
12	24.61180203764164	24.81347679682087	29.04952294166651	21.52519822387098
13	29.419480143785314	21.148427627852378	25.121786958220245	24.310305270142063
14	23.442635680023855	20.120562226495867	26.296245469513803	30.14055662396648
15	24.533396596717495	27.954337136325403	22.88653568270537	24.62573058425173
16	24.3812820975646	27.29487181733905	24.315592334154243	24.008253750942107
17	23.472901045200917	26.890327099440075	25.445111517483777	24.191660337875234
18	23.42669183981294	27.006558068376265	26.438881509871077	23.127868581939723
19	24.951402947922322	25.808698221844683	26.192176450945055	23.047722379287944
20	25.704043250239305	25.523087916088066	25.68062502527874	23.09224380839389
21	25.982967513707283	25.062655121137727	25.20460149455551	23.74977587059948
22	25.4216669951759	25.487687914248575	25.515420205556005	23.575224885019523
23	24.751068256367507	25.642197586002847	25.506563104481828	24.100171053147818
24	24.247107745090055	26.02301400653275	25.73091688781346	23.998961360563733
25	24.494091117997925	25.772249090751963	25.68797419794259	24.045685593307514
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	163.0
1	163.0
2	583.5
3	1004.0
4	1004.0
5	1004.0
6	2473.5
7	3943.0
8	3943.0
9	3943.0
10	5475.0
11	7007.0
12	7007.0
13	7007.0
14	11024.0
15	15041.0
16	15041.0
17	15041.0
18	30527.5
19	46014.0
20	46014.0
21	46014.0
22	73633.0
23	101252.0
24	101252.0
25	101252.0
26	153009.5
27	204767.0
28	204767.0
29	204767.0
30	277037.0
31	349307.0
32	349307.0
33	349307.0
34	432391.5
35	515476.0
36	515476.0
37	515476.0
38	612348.0
39	709220.0
40	709220.0
41	709220.0
42	801878.0
43	894536.0
44	894536.0
45	894536.0
46	972441.0
47	1050346.0
48	1050346.0
49	1050346.0
50	1063638.0
51	1076930.0
52	1076930.0
53	1076930.0
54	990934.0
55	904938.0
56	904938.0
57	904938.0
58	786204.0
59	667470.0
60	667470.0
61	667470.0
62	551908.5
63	436347.0
64	436347.0
65	436347.0
66	340645.5
67	244944.0
68	244944.0
69	244944.0
70	178392.5
71	111841.0
72	111841.0
73	111841.0
74	79799.5
75	47758.0
76	47758.0
77	47758.0
78	33868.0
79	19978.0
80	19978.0
81	19978.0
82	13691.5
83	7405.0
84	7405.0
85	7405.0
86	5038.0
87	2671.0
88	2671.0
89	2671.0
90	1915.0
91	1159.0
92	1159.0
93	1159.0
94	895.0
95	631.0
96	631.0
97	631.0
98	1826.0
99	3021.0
100	3021.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.038716618199046794
2	0.005038279473362387
3	0.0014683755684398402
4	0.003771973937276654
5	0.009254807481818075
6	0.013835061548511156
7	0.022793499649543206
8	0.036224421133346146
9	0.0492916165589117
10	0.05806145596307992
11	0.05513817616169052
12	0.06451422566292106
13	0.06583441654096789
14	0.06858256898098374
15	0.05993397159622797
16	0.06689865204469951
17	0.06319403478487422
18	0.07733893704966167
19	0.0746042559451361
20	0.07906326799241672
21	0.07515658070023733
22	0.07844358655986412
23	0.08319896798793076
24	0.07777001978535043
25	0.07781043379182126
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	7423169.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.233840691182266
#Duplication Level	Percentage of deduplicated	Percentage of total
1	58.641714676349046	20.075311180878984
2	15.49770096873096	10.610916520862336
3	7.906368678682741	8.119960973753345
4	4.665100145894078	6.388171808117961
5	3.045683995185218	5.213273034342715
6	2.1046146318822743	4.322942521451339
7	1.5237923661370318	3.6515685576142376
8	1.1696739808071106	3.2033946175657286
9	0.8977710189208744	2.7660735035007837
>10	4.307547012751663	24.82158228669075
>50	0.16130080464865415	3.7547024050834708
>100	0.07288930987736504	4.575721725938814
>500	0.0038292718050180804	0.9010633725765377
>1k	0.0018947184280626067	1.2959995224077119
>5k	1.1841989968094704E-4	0.299317969215349
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	9940	0.1339050747733212	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.3471335490273763E-5
2	2.6942670980547525E-5	0.0	0.0	0.0	1.3471335490273763E-5
3	2.6942670980547525E-5	0.0	0.0	0.0	1.3471335490273763E-5
4	2.6942670980547525E-5	0.0	0.0	0.0	1.3471335490273763E-5
5	4.041400647082129E-5	0.0	0.0	0.0	1.3471335490273763E-5
6	6.735667745136882E-5	1.3471335490273763E-5	0.0	0.0	5.388534196109505E-5
7	6.735667745136882E-5	1.3471335490273763E-5	0.0	1.3471335490273763E-5	5.388534196109505E-5
8	1.7512736137355892E-4	1.3471335490273763E-5	0.0	1.3471335490273763E-5	8.082801294164258E-5
9	1.885986968638327E-4	1.3471335490273763E-5	0.0	6.735667745136882E-5	9.429934843191635E-5
10	1.885986968638327E-4	1.3471335490273763E-5	0.0	8.082801294164258E-5	9.429934843191635E-5
11	2.155413678443802E-4	1.3471335490273763E-5	0.0	8.082801294164258E-5	9.429934843191635E-5
12	2.155413678443802E-4	1.3471335490273763E-5	0.0	1.3471335490273763E-4	9.429934843191635E-5
13	2.155413678443802E-4	1.3471335490273763E-5	0.0	1.7512736137355892E-4	1.077706839221901E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	3340	0.0	15.494981	1
GTATCAA	12280	0.0	13.702706	1
CGCGTAA	120	7.403287E-10	12.668087	10
TCGCGTA	125	1.4115358E-9	12.1611185	9
GCGGTAA	130	3.2627213E-8	10.963951	15
ACCGCGC	110	6.048338E-6	10.3644495	8
TAGGACC	1195	0.0	9.934267	4
ACCGTAC	135	6.4469714E-7	9.852625	8
GCACCGT	340	0.0	9.7783985	6
GTATTAG	645	0.0	9.569619	1
GTAGGAC	1345	0.0	9.531179	3
TATCAAC	17845	0.0	9.3856535	2
GACCGCG	285	0.0	9.333373	7
GCGATTC	210	6.675691E-10	9.048635	13
CCTATAC	715	0.0	9.031045	3
GTCGCGT	200	2.6593625E-9	9.025708	8
ATCAACG	18590	0.0	8.9697485	3
TGCGATT	255	3.6379788E-12	8.94242	12
TCAACGC	18735	0.0	8.871112	4
GCGTAAC	150	2.7013866E-6	8.867721	11
>>END_MODULE