FastQCFastQC Report
Thu 2 Feb 2017
SRR4063878_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063878_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7423169
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA39800.030.4551891
GTATCAA123500.029.8181881
TATCAAC188850.019.428912
ATCAACG193200.018.8779813
TCAACGC195400.018.620534
CAACGCA201600.018.0696985
AACGCAG207900.017.5539916
GTAGGAC19400.014.7407563
ACGCAGA253000.014.4248997
CGCAGAG260300.014.0033628
TAGGACC18900.013.9669164
AGAGTAC264050.013.87216511
TAGGACG8950.013.7640454
GTATAGG11950.013.6224931
ACGACGT1952.4118162E-713.53816741
CGACGTT1952.4125984E-713.53780342
CAGAGTA272350.013.49796110
ACGTCGA1150.001425160213.39119315
GTATTAG11450.013.2567281
AGTACAT248750.012.98303413
Produced by FastQC (version 0.11.5)