##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063878_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7423169
Sequences flagged as poor quality	0
Sequence length	50
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.35678468319932	32.0	32.0	32.0	32.0	32.0
2	31.451666532177835	32.0	32.0	32.0	32.0	32.0
3	31.508669141171378	32.0	32.0	32.0	32.0	32.0
4	31.583872602119122	32.0	32.0	32.0	32.0	32.0
5	31.551113682040647	32.0	32.0	32.0	32.0	32.0
6	35.14017315246359	36.0	36.0	36.0	36.0	36.0
7	35.15916773550488	36.0	36.0	36.0	36.0	36.0
8	35.109425772200524	36.0	36.0	36.0	36.0	36.0
9	35.204076183635316	36.0	36.0	36.0	36.0	36.0
10	35.084546505677025	36.0	36.0	36.0	36.0	36.0
11	35.20642477626469	36.0	36.0	36.0	36.0	36.0
12	35.132520625624984	36.0	36.0	36.0	36.0	36.0
13	35.16005361052672	36.0	36.0	36.0	36.0	36.0
14	35.10818937302923	36.0	36.0	36.0	36.0	36.0
15	35.08982363192863	36.0	36.0	36.0	36.0	36.0
16	35.104433968834606	36.0	36.0	36.0	36.0	36.0
17	35.076305685617555	36.0	36.0	36.0	36.0	36.0
18	35.071206380994425	36.0	36.0	36.0	36.0	36.0
19	35.06001385661568	36.0	36.0	36.0	36.0	36.0
20	35.05588960725534	36.0	36.0	36.0	36.0	36.0
21	35.0511460806025	36.0	36.0	36.0	36.0	36.0
22	35.03286978916956	36.0	36.0	36.0	36.0	36.0
23	34.98591464103808	36.0	36.0	36.0	36.0	36.0
24	34.97228690334276	36.0	36.0	36.0	36.0	36.0
25	34.940143623296194	36.0	36.0	36.0	36.0	36.0
26	34.88660745835101	36.0	36.0	36.0	36.0	36.0
27	34.868440284735534	36.0	36.0	36.0	32.0	36.0
28	34.85476997762007	36.0	36.0	36.0	32.0	36.0
29	34.82981217321066	36.0	36.0	36.0	32.0	36.0
30	34.815112925490446	36.0	36.0	36.0	32.0	36.0
31	34.8013702773034	36.0	36.0	36.0	32.0	36.0
32	34.768187414297046	36.0	36.0	36.0	32.0	36.0
33	34.75494266666972	36.0	36.0	36.0	32.0	36.0
34	34.74521690129916	36.0	36.0	36.0	32.0	36.0
35	34.71758746163532	36.0	36.0	36.0	32.0	36.0
36	34.69083891259919	36.0	36.0	36.0	32.0	36.0
37	34.67724539209602	36.0	36.0	36.0	32.0	36.0
38	34.65665472522584	36.0	36.0	36.0	32.0	36.0
39	34.644979118756424	36.0	36.0	36.0	32.0	36.0
40	34.6348944770084	36.0	36.0	36.0	32.0	36.0
41	34.6172498564966	36.0	36.0	36.0	32.0	36.0
42	34.57947151681445	36.0	36.0	36.0	32.0	36.0
43	34.561273763267415	36.0	36.0	36.0	32.0	36.0
44	34.528099791342484	36.0	36.0	36.0	32.0	36.0
45	34.49956709863402	36.0	36.0	36.0	32.0	36.0
46	34.48491338402776	36.0	36.0	36.0	32.0	36.0
47	34.45257126706936	36.0	36.0	36.0	32.0	36.0
48	34.424661219487255	36.0	36.0	36.0	32.0	36.0
49	34.407157643858035	36.0	36.0	36.0	32.0	36.0
50	33.84319486731341	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	0.0
16	0.0
17	7.0
18	8.0
19	18.0
20	93.0
21	311.0
22	945.0
23	2644.0
24	6602.0
25	14011.0
26	27495.0
27	49157.0
28	80197.0
29	121495.0
30	172850.0
31	243175.0
32	346843.0
33	549705.0
34	1165335.0
35	4642277.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.94561165067379	18.269546857165643	12.64765970151184	26.13718179064872
2	15.23252436705996	20.716648597035	38.286055920301074	25.764771115603974
3	17.844139605016366	24.9504152082211	28.60638680946871	28.599058377293822
4	12.181118333692794	17.1557996322056	35.897875961061914	34.76520607303969
5	13.801356267114489	37.70485354704978	33.50353467636262	14.99025550947311
6	32.8616784725199	36.25334999112238	17.77332115162908	13.111650384728648
7	30.463326626849867	30.885222139074102	20.493253214767556	18.158198019308475
8	28.022177057312597	33.811956540921145	19.030408449654978	19.135457952111278
9	27.938837778770775	15.00101238313643	18.40427671590185	38.65587312219094
10	15.222200039175082	28.795301576729678	32.390544848867435	23.591953535227802
11	35.70843819086921	22.26689767318989	22.610080037267114	19.41458409867379
12	24.58788087063227	24.856499734546905	29.020496265941837	21.535123128878993
13	29.512718468810107	21.088442064020867	25.15556081238915	24.243278654779875
14	23.507025520563975	20.07006189150672	26.21227831684954	30.210634271079762
15	24.559348916173807	28.002904016436876	22.81459493241318	24.623152134976138
16	24.48579819743943	27.306773295871935	24.24030800041007	23.96712050627857
17	23.538868642220052	26.90985480729322	25.378621987455762	24.172654563030964
18	23.52693857833323	27.01197039637911	26.390023852420523	23.07106717286714
19	25.025531555466824	25.813820435401762	26.149580091623964	23.011067917507447
20	25.82337257285608	25.49372717269593	25.62421124895058	23.058689005497413
21	26.02943567632638	25.101099005020632	25.165747944038458	23.703717374614534
22	25.516717482871503	25.449832194038198	25.48473648071838	23.548713842371917
23	24.855805066096952	25.57616341719799	25.45432770707591	24.113703809629143
24	24.370609163519973	26.060583608262284	25.593085396591462	23.97572183162628
25	24.589743444665107	25.68575022787166	25.69779381433855	24.02671251312468
26	24.172491094956595	26.00218786543011	25.776557506911175	24.04876353270212
27	24.344112345772654	26.024950129514394	25.41256063977565	24.2183768849373
28	24.239899284171273	25.824220162969418	25.752697913810906	24.1831826390484
29	24.273767442574776	25.941893312421755	25.488464840567854	24.29587440443562
30	24.152367239362256	26.0563784435965	25.641877819312864	24.149376497728383
31	24.515761716489706	25.5282455547806	25.662227588389406	24.293765140340287
32	24.21221681284907	25.98759679145048	25.706359682270502	24.093826713429948
33	24.220436832625992	25.85485103214496	25.649700525795843	24.275011609433207
34	24.302475023440785	25.840298209878537	25.95866069600267	23.898566070678005
35	24.236325638784848	25.82357633294854	25.845292734260628	24.09480529400599
36	24.184661174153653	25.799704119411704	25.975761287604637	24.03987341883001
37	24.472866752904462	25.421419647290826	25.71053900059896	24.395174599205756
38	24.286185049892342	25.854668900929624	25.750042235976643	24.10910381320139
39	24.392690529247275	25.744298392682175	25.68293392994051	24.180077148130042
40	24.376121346119184	25.64469234667718	25.891843160943868	24.087343146259773
41	24.26332551924654	25.645760111352423	25.925879024141313	24.165035345259728
42	24.106014972059242	25.652998433118995	25.96828760019329	24.27269899462847
43	24.114457641425837	25.389723777656652	26.045476014127832	24.45034256678968
44	23.834133651978927	25.523431295748335	26.137461715715233	24.50497333655751
45	23.919647435042517	25.48708178781709	26.129375302940044	24.463895474200353
46	23.912093448702766	25.44131772262569	26.07443510873475	24.572153719936793
47	23.86339234353719	25.52316378396365	26.222233298890878	24.39121057360828
48	23.798904326463617	25.731704129885784	26.116561278208554	24.352830265442044
49	23.935301737702233	25.4050040306415	26.0977301237644	24.561964107891868
50	23.584392980262884	25.779010571097942	26.225092852418204	24.41150359622097
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3.0
1	30.0
2	57.0
3	170.0
4	283.0
5	389.5
6	496.0
7	538.0
8	580.0
9	758.5
10	937.0
11	1385.5
12	1834.0
13	3569.0
14	5304.0
15	6983.5
16	8663.0
17	8642.5
18	8622.0
19	8801.5
20	8981.0
21	10783.0
22	12585.0
23	17003.5
24	21422.0
25	29019.0
26	36616.0
27	47681.5
28	58747.0
29	74927.0
30	91107.0
31	114823.0
32	138539.0
33	166007.5
34	193476.0
35	225498.5
36	257521.0
37	288796.0
38	320071.0
39	355042.5
40	390014.0
41	427306.0
42	464598.0
43	488254.5
44	511911.0
45	542224.0
46	572537.0
47	593519.5
48	614502.0
49	618923.5
50	623345.0
51	618546.0
52	613747.0
53	590044.0
54	566341.0
55	530995.5
56	495650.0
57	455565.0
58	415480.0
59	369997.0
60	324514.0
61	276808.0
62	229102.0
63	194671.5
64	160241.0
65	134620.0
66	108999.0
67	89689.5
68	70380.0
69	55258.5
70	40137.0
71	30640.5
72	21144.0
73	17446.5
74	13749.0
75	11110.0
76	8471.0
77	7009.5
78	5548.0
79	4379.0
80	3210.0
81	2502.0
82	1794.0
83	1340.5
84	887.0
85	671.5
86	456.0
87	336.5
88	217.0
89	160.5
90	104.0
91	85.5
92	67.0
93	62.5
94	58.0
95	61.0
96	64.0
97	52.5
98	41.0
99	29.0
100	17.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009389520836720813
2	4.445540711790342E-4
3	3.5025472274711783E-4
4	0.0
5	0.0
6	2.0207003235410642E-4
7	5.388534196109505E-5
8	2.6942670980547525E-5
9	0.0012124201941246388
10	0.0011181208456927222
11	2.6942670980547525E-5
12	1.6165602588328516E-4
13	3.637260582373916E-4
14	2.424840388249277E-4
15	0.004661082079634722
16	8.352228003969732E-4
17	0.0
18	3.0984071627629655E-4
19	2.6942670980547525E-5
20	5.523247551012244E-4
21	0.0
22	1.6165602588328516E-4
23	7.813374584358783E-4
24	5.253820841206768E-4
25	0.0017647449492258628
26	0.005509776215521969
27	0.005954330286701003
28	0.004095285989043224
29	0.0022631843623659924
30	0.003516018562961452
31	0.007166750480825643
32	0.0033813052080587146
33	0.004391655369829247
34	0.0028155091174672164
35	0.0028020377819769427
36	0.007813374584358782
37	0.0038123879437474747
38	0.0079076739327907
39	0.004122228660023771
40	0.0023035983688368135
41	0.003367833872568441
42	0.0019129296396188744
43	0.0012258915296149123
44	0.0011315921811829962
45	0.0011181208456927222
46	7.948087939261521E-4
47	0.0021419423429535284
48	0.002088057000992433
49	4.445540711790342E-4
50	0.0013336622135371025
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	7423169.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.910434429454426
#Duplication Level	Percentage of deduplicated	Percentage of total
1	61.22687890307499	23.21137577975171
2	15.141056113973772	11.480080300029854
3	7.387319944071316	8.401695250473496
4	4.321001434979034	6.552441662814047
5	2.769485683557997	5.249620270491911
6	1.9699645667661965	4.48093275220431
7	1.404971432960119	3.7284154169143857
8	1.0313804489191036	3.1280064704455155
9	0.7698550474279845	2.626698536613282
>10	3.8006638449292	23.96650424395253
>50	0.12146986911819753	3.1238199982946315
>100	0.054342015251209755	3.6062179440146447
>500	0.0014317548181996638	0.3489618099465583
>1k	1.7894015256444653E-4	0.09522956405322418
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.6942670980547525E-5
2	2.6942670980547525E-5	0.0	0.0	0.0	2.6942670980547525E-5
3	2.6942670980547525E-5	0.0	0.0	0.0	2.6942670980547525E-5
4	2.6942670980547525E-5	0.0	0.0	0.0	5.388534196109505E-5
5	2.6942670980547525E-5	0.0	0.0	0.0	5.388534196109505E-5
6	4.041400647082129E-5	0.0	0.0	0.0	5.388534196109505E-5
7	4.041400647082129E-5	0.0	0.0	1.3471335490273763E-5	6.735667745136882E-5
8	1.7512736137355892E-4	0.0	0.0	1.3471335490273763E-5	6.735667745136882E-5
9	1.7512736137355892E-4	0.0	0.0	6.735667745136882E-5	8.082801294164258E-5
10	1.7512736137355892E-4	0.0	0.0	8.082801294164258E-5	8.082801294164258E-5
11	1.885986968638327E-4	0.0	0.0	9.429934843191635E-5	8.082801294164258E-5
12	1.885986968638327E-4	0.0	0.0	1.7512736137355892E-4	8.082801294164258E-5
13	1.885986968638327E-4	0.0	0.0	2.2901270333465398E-4	9.429934843191635E-5
14	2.155413678443802E-4	0.0	0.0	2.6942670980547527E-4	1.3471335490273763E-4
15	2.2901270333465398E-4	0.0	0.0	3.906687292179391E-4	1.885986968638327E-4
16	2.2901270333465398E-4	0.0	0.0	4.8496807764985547E-4	2.155413678443802E-4
17	4.310827356887604E-4	0.0	0.0	4.984394131401292E-4	2.155413678443802E-4
18	4.310827356887604E-4	0.0	0.0	6.331527680428668E-4	2.155413678443802E-4
19	4.5802540666930796E-4	0.0	0.0	6.600954390234144E-4	2.155413678443802E-4
20	4.5802540666930796E-4	0.0	0.0	9.160508133386159E-4	2.155413678443802E-4
21	4.714967421595817E-4	0.0	0.0	9.968788262802585E-4	2.4248403882492773E-4
22	4.8496807764985547E-4	0.0	0.0	0.0013336622135371025	2.4248403882492773E-4
23	4.8496807764985547E-4	0.0	0.0	0.0016030889233425779	2.8289804529574905E-4
24	4.8496807764985547E-4	0.0	0.0	0.0020745856655021593	2.9636938078602277E-4
25	5.11910748630403E-4	0.0	0.0	0.0024248403882492775	2.9636938078602277E-4
26	5.11910748630403E-4	0.0	0.0	0.002909808465899133	3.0984071627629655E-4
27	6.331527680428668E-4	0.0	0.0	0.00365073191786419	3.2331205176657033E-4
28	6.331527680428668E-4	0.0	0.0	0.00565796090591498	3.2331205176657033E-4
29	7.543947874553308E-4	0.0	0.0	0.008998852107502875	3.2331205176657033E-4
30	7.543947874553308E-4	0.0	0.0	0.01771480616971	3.2331205176657033E-4
31	7.543947874553308E-4	0.0	0.0	0.0454388146086934	3.5025472274711783E-4
32	7.678661229456045E-4	0.0	0.0	0.0773254657141714	3.637260582373916E-4
33	7.678661229456045E-4	0.0	0.0	0.1108017344075017	3.637260582373916E-4
34	8.082801294164258E-4	0.0	0.0	0.14877742915458345	3.637260582373916E-4
35	8.756368068677946E-4	1.3471335490273763E-5	0.0	0.19301729490464248	3.637260582373916E-4
36	9.160508133386159E-4	1.3471335490273763E-5	0.0	0.2582589726840383	3.637260582373916E-4
37	9.160508133386159E-4	1.3471335490273763E-5	0.0	0.36368564423092076	3.637260582373916E-4
38	9.160508133386159E-4	1.3471335490273763E-5	0.0	0.5170702701231779	3.637260582373916E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	3980	0.0	30.455189	1
GTATCAA	12350	0.0	29.818188	1
TATCAAC	18885	0.0	19.42891	2
ATCAACG	19320	0.0	18.877981	3
TCAACGC	19540	0.0	18.62053	4
CAACGCA	20160	0.0	18.069698	5
AACGCAG	20790	0.0	17.553991	6
GTAGGAC	1940	0.0	14.740756	3
ACGCAGA	25300	0.0	14.424899	7
CGCAGAG	26030	0.0	14.003362	8
TAGGACC	1890	0.0	13.966916	4
AGAGTAC	26405	0.0	13.872165	11
TAGGACG	895	0.0	13.764045	4
GTATAGG	1195	0.0	13.622493	1
ACGACGT	195	2.4118162E-7	13.538167	41
CGACGTT	195	2.4125984E-7	13.537803	42
CAGAGTA	27235	0.0	13.497961	10
ACGTCGA	115	0.0014251602	13.391193	15
GTATTAG	1145	0.0	13.256728	1
AGTACAT	24875	0.0	12.983034	13
>>END_MODULE