##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063877_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	13833
Sequences flagged as poor quality	0
Sequence length	25
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.350177112701513	32.0	32.0	32.0	32.0	32.0
2	30.99840960023133	32.0	32.0	32.0	32.0	32.0
3	31.08335140605798	32.0	32.0	32.0	32.0	32.0
4	31.059278536832213	32.0	32.0	32.0	32.0	32.0
5	31.060218318513698	32.0	32.0	32.0	32.0	32.0
6	34.71857153184414	36.0	36.0	36.0	32.0	36.0
7	34.61938841899805	36.0	36.0	36.0	32.0	36.0
8	34.61938841899805	36.0	36.0	36.0	32.0	36.0
9	34.65141328706716	36.0	36.0	36.0	32.0	36.0
10	34.54348297549338	36.0	36.0	36.0	32.0	36.0
11	34.638473216222074	36.0	36.0	36.0	32.0	36.0
12	34.56603773584906	36.0	36.0	36.0	32.0	36.0
13	34.57572471625822	36.0	36.0	36.0	32.0	36.0
14	34.524976505457964	36.0	36.0	36.0	32.0	36.0
15	34.546952938625026	36.0	36.0	36.0	32.0	36.0
16	34.4433600809658	36.0	36.0	36.0	32.0	36.0
17	34.51680763391889	36.0	36.0	36.0	32.0	36.0
18	34.439311790645554	36.0	36.0	36.0	32.0	36.0
19	34.388635870743876	36.0	36.0	36.0	32.0	36.0
20	34.33636955107352	36.0	36.0	36.0	32.0	36.0
21	34.38198510807489	36.0	36.0	36.0	32.0	36.0
22	34.31822453553097	36.0	36.0	36.0	32.0	36.0
23	34.31511602689221	36.0	36.0	36.0	32.0	36.0
24	34.29227210294224	36.0	36.0	36.0	32.0	36.0
25	33.94484204438661	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	1.0
12	1.0
13	1.0
14	0.0
15	3.0
16	1.0
17	4.0
18	11.0
19	8.0
20	9.0
21	20.0
22	28.0
23	42.0
24	61.0
25	75.0
26	142.0
27	173.0
28	227.0
29	323.0
30	416.0
31	561.0
32	782.0
33	1136.0
34	2635.0
35	7171.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.1246655578856	18.294887555137755	11.859136597006291	25.72131028997035
2	16.908841176895827	21.578833224896986	38.14067808862865	23.371647509578544
3	19.786018940215428	24.029494686618953	30.022410178558516	26.1620761946071
4	14.16281087333719	17.813765182186234	38.44707923655292	29.576344707923656
5	14.732885129762163	35.89243114291911	35.45145666160631	13.923227065712426
6	31.09954456733897	35.711703896479435	19.728186221354733	13.460565314826864
7	28.27187274041938	31.10629067245119	22.443962400578453	18.177874186550977
8	24.80838756326826	35.3000723065799	22.530730296456976	17.360809833694866
9	26.372116566635334	17.058355629474292	21.071661002241665	35.49786680164871
10	15.781860263272096	28.02690582959641	34.760595978591056	21.43063792854043
11	33.14529543646489	22.535618717002965	25.58038620091126	18.738699645620887
12	23.51707175925926	24.32002314814815	31.45978009259259	20.703125
13	28.44122965641953	22.70524412296564	25.909584086799274	22.94394213381555
14	22.404687839108732	22.404687839108732	27.34572813426897	27.844896187513562
15	23.947634890785476	29.958050050629247	23.11586865326197	22.978446405323304
16	23.40348593331887	27.750054241701022	26.911115932595646	21.935343892384466
17	22.701959933463513	27.91639545816157	27.287191726332537	22.094452882042383
18	22.73582175925926	27.727141203703702	28.132233796296298	21.40480324074074
19	23.56600361663653	27.11754068716094	27.471971066907773	21.844484629294755
20	23.424726904434635	27.787021630615637	27.114229906677277	21.674021558272443
21	23.66898148148148	27.12673611111111	26.33101851851852	22.87326388888889
22	22.352430555555554	29.166666666666668	27.061631944444443	21.419270833333336
23	22.795341098169715	27.902770744411487	27.30232221659553	21.999565940823267
24	23.05077390423839	27.375958339360622	27.51338058729929	22.059887169101692
25	23.370705244122966	27.283905967450274	27.956600361663654	21.38878842676311
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1.0
1	1.0
2	2.0
3	3.0
4	3.0
5	3.0
6	5.5
7	8.0
8	8.0
9	8.0
10	20.0
11	32.0
12	32.0
13	32.0
14	43.0
15	54.0
16	54.0
17	54.0
18	110.0
19	166.0
20	166.0
21	166.0
22	271.5
23	377.0
24	377.0
25	377.0
26	495.0
27	613.0
28	613.0
29	613.0
30	828.5
31	1044.0
32	1044.0
33	1044.0
34	1203.0
35	1362.0
36	1362.0
37	1362.0
38	1475.0
39	1588.0
40	1588.0
41	1588.0
42	1686.0
43	1784.0
44	1784.0
45	1784.0
46	1804.5
47	1825.0
48	1825.0
49	1825.0
50	1696.5
51	1568.0
52	1568.0
53	1568.0
54	1395.5
55	1223.0
56	1223.0
57	1223.0
58	1064.0
59	905.0
60	905.0
61	905.0
62	741.5
63	578.0
64	578.0
65	578.0
66	429.5
67	281.0
68	281.0
69	281.0
70	202.5
71	124.0
72	124.0
73	124.0
74	99.0
75	74.0
76	74.0
77	74.0
78	58.5
79	43.0
80	43.0
81	43.0
82	31.0
83	19.0
84	19.0
85	19.0
86	12.0
87	5.0
88	5.0
89	5.0
90	6.0
91	7.0
92	7.0
93	7.0
94	4.0
95	1.0
96	1.0
97	1.0
98	74.5
99	148.0
100	148.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.02891635943034772
2	0.0
3	0.0
4	0.00722908985758693
5	0.0
6	0.0
7	0.02168726957276079
8	0.02168726957276079
9	0.02891635943034772
10	0.050603629003108504
11	0.04337453914552158
12	0.06506180871828238
13	0.05783271886069544
14	0.07229089857586929
15	0.050603629003108504
16	0.04337453914552158
17	0.04337453914552158
18	0.06506180871828238
19	0.05783271886069544
20	0.07229089857586929
21	0.06506180871828238
22	0.06506180871828238
23	0.07229089857586929
24	0.050603629003108504
25	0.05783271886069544
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	13833.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	93.33477915130484
#Duplication Level	Percentage of deduplicated	Percentage of total
1	95.01975060026334	88.68647437287646
2	4.523274726976997	8.443576953661534
3	0.24785066997134228	0.6939926263283452
4	0.0851986678026489	0.3180799537338249
5	0.054217334056231116	0.25301814501554254
6	0.015490666873208893	0.08674907829104316
7	0.007745333436604446	0.050603629003108504
8	0.007745333436604446	0.05783271886069544
9	0.007745333436604446	0.06506180871828238
>10	0.023236000309813338	0.3325381334489988
>50	0.0	0.0
>100	0.007745333436604446	1.0120725800621702
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGGGGGGGGGGGGGGGGGGGGGGGG	140	1.0120725800621702	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	20	0.14458179715173858	No Hit
CTTTAGGACGTGAAATATGGCGAGG	14	0.10120725800621701	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE