##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063877_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	13833
Sequences flagged as poor quality	0
Sequence length	50
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.316995590255186	32.0	32.0	32.0	32.0	32.0
2	31.412130412781032	32.0	32.0	32.0	32.0	32.0
3	31.454348297549338	32.0	32.0	32.0	32.0	32.0
4	31.5509289380467	32.0	32.0	32.0	32.0	32.0
5	31.587435841827514	32.0	32.0	32.0	32.0	32.0
6	35.133087544278176	36.0	36.0	36.0	36.0	36.0
7	35.1725583749006	36.0	36.0	36.0	36.0	36.0
8	35.19402877177763	36.0	36.0	36.0	36.0	36.0
9	35.19475168076339	36.0	36.0	36.0	36.0	36.0
10	35.083712860550854	36.0	36.0	36.0	36.0	36.0
11	35.216583532133306	36.0	36.0	36.0	36.0	36.0
12	35.12123183691173	36.0	36.0	36.0	36.0	36.0
13	35.13279838068387	36.0	36.0	36.0	36.0	36.0
14	35.06072435480373	36.0	36.0	36.0	36.0	36.0
15	35.05457962842478	36.0	36.0	36.0	36.0	36.0
16	35.04250704836261	36.0	36.0	36.0	36.0	36.0
17	35.09990602183185	36.0	36.0	36.0	36.0	36.0
18	35.08313453336225	36.0	36.0	36.0	36.0	36.0
19	35.07944769753488	36.0	36.0	36.0	36.0	36.0
20	35.056603773584904	36.0	36.0	36.0	36.0	36.0
21	35.001590399768666	36.0	36.0	36.0	36.0	36.0
22	35.086243042001016	36.0	36.0	36.0	36.0	36.0
23	35.00079519988434	36.0	36.0	36.0	36.0	36.0
24	34.908552013301524	36.0	36.0	36.0	36.0	36.0
25	34.91238343092605	36.0	36.0	36.0	36.0	36.0
26	34.869008891780524	36.0	36.0	36.0	32.0	36.0
27	34.904648304778426	36.0	36.0	36.0	36.0	36.0
28	34.83235740620256	36.0	36.0	36.0	32.0	36.0
29	34.79346490276874	36.0	36.0	36.0	32.0	36.0
30	34.74676498228873	36.0	36.0	36.0	32.0	36.0
31	34.72037880430854	36.0	36.0	36.0	32.0	36.0
32	34.65885924962047	36.0	36.0	36.0	32.0	36.0
33	34.6941372081255	36.0	36.0	36.0	32.0	36.0
34	34.7111978601894	36.0	36.0	36.0	32.0	36.0
35	34.59661678594665	36.0	36.0	36.0	32.0	36.0
36	34.641870888455145	36.0	36.0	36.0	32.0	36.0
37	34.580423624665656	36.0	36.0	36.0	32.0	36.0
38	34.57861635220126	36.0	36.0	36.0	32.0	36.0
39	34.47148124051182	36.0	36.0	36.0	32.0	36.0
40	34.56748355382057	36.0	36.0	36.0	32.0	36.0
41	34.474878912744884	36.0	36.0	36.0	32.0	36.0
42	34.54239861201475	36.0	36.0	36.0	32.0	36.0
43	34.47227644039615	36.0	36.0	36.0	32.0	36.0
44	34.43208270078797	36.0	36.0	36.0	32.0	36.0
45	34.44292633557435	36.0	36.0	36.0	32.0	36.0
46	34.39499746981855	36.0	36.0	36.0	32.0	36.0
47	34.3828525988578	36.0	36.0	36.0	32.0	36.0
48	34.36644256488108	36.0	36.0	36.0	32.0	36.0
49	34.344466131714015	36.0	36.0	36.0	32.0	36.0
50	33.86264729270585	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	1.0
21	0.0
22	6.0
23	5.0
24	15.0
25	24.0
26	65.0
27	113.0
28	171.0
29	225.0
30	340.0
31	491.0
32	636.0
33	949.0
34	2130.0
35	8662.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	41.01952277657267	20.535068691250906	11.814895155459148	26.63051337671728
2	15.26060868936601	21.600520494469748	39.30456155570013	23.834309260464106
3	17.082339333477915	25.793392611870164	30.282657413431647	26.84161064122027
4	12.108725511458108	20.024578905515796	38.502132581507986	29.364563001518107
5	13.11356900166269	36.86835827369334	35.74062025590978	14.277452468734186
6	30.051326537988864	36.86835827369334	19.71372804163956	13.366587146678233
7	27.75970505313381	31.403166341357625	22.851153039832283	17.98597556567628
8	24.947589098532493	35.400853032603194	22.50415672666811	17.1474011421962
9	25.43193811899082	17.002819345044458	20.017349815658207	37.547892720306514
10	15.007590544350466	29.480228439239497	34.60565314826864	20.9065278681414
11	33.340562423190924	22.627051254247093	25.518687197281864	18.513699125280127
12	23.602978385021327	25.417479939275644	31.012795489047928	19.966746186655097
13	28.432010409889397	22.533073086098458	25.4969999277091	23.537916576303044
14	20.877611508711052	23.09694209499024	27.145232415238922	28.880213981059782
15	23.597455176402544	29.952284557547713	22.78773857721226	23.66252168883748
16	22.851153039832283	28.525988578038024	26.81992337164751	21.80293501048218
17	21.752331381479074	29.140461215932913	27.231981493529965	21.87522590905805
18	22.446324007807416	26.84161064122027	29.104315766644977	21.607749584327333
19	23.451167498012	27.304272392105833	26.827152461505094	22.41740764837707
20	22.453553097665004	28.034410467722115	27.897057760427963	21.614978674184922
21	22.55476035567122	27.39825056025446	26.335574351189184	23.71141473288513
22	22.706571242680546	29.726017494397457	26.357261620761946	21.210149642160054
23	22.663196703535025	27.954890479288657	26.241596183040556	23.140316634135765
24	22.995734836984024	27.586206896551722	27.56451962697896	21.85353863948529
25	22.41740764837707	27.730788693703463	27.369334200824113	22.482469457095352
26	23.243204164256795	27.92799305957201	27.147194910352805	21.681607865818393
27	22.164389503361527	27.774163232848988	26.827152461505094	23.234294802284392
28	21.882454998915637	28.757319453480807	26.747632473071643	22.612593074531915
29	22.402949468661895	27.933203209715895	26.935588809368898	22.72825851225331
30	22.807778500686762	27.354876021108943	27.470541458830333	22.366804019373962
31	23.26321116171474	26.7693197426444	28.36694860117111	21.600520494469748
32	22.30174221065568	27.506686908118265	27.44885418925757	22.742716691968482
33	22.288895315211104	27.472527472527474	28.021978021978022	22.2165991902834
34	21.723415022048727	27.788621412564158	27.369334200824113	23.118629364563002
35	22.25113858165257	27.369334200824113	28.540446757753195	21.839080459770116
36	22.366804019373962	27.998265018434175	27.889828670570378	21.745102291621485
37	22.5113858165257	27.48499963854551	27.405479650112053	22.59813489481674
38	22.265596761367743	28.359719511313525	27.636810525554832	21.7378732017639
39	22.952360297838503	26.190992554037447	27.586206896551722	23.270440251572328
40	22.525843996240873	26.978963348514423	28.706715824477698	21.788476830767006
41	22.966818477553677	26.42955251933782	27.680185064700353	22.923443938408155
42	22.280054941082916	27.362105110966528	27.39825056025446	22.959589387696088
43	22.0125786163522	27.362105110966528	28.171763175016267	22.453553097665004
44	21.578833224896986	27.24643967324514	28.598279476613897	22.576447625243983
45	22.706571242680546	27.05848333694788	27.839225041567268	22.39572037880431
46	22.670425793392614	26.610279765777488	28.395864960601457	22.32342948022844
47	22.88729848912022	26.082556206173642	28.085014096725224	22.945131207980914
48	21.91860044820357	25.778934432154994	29.1476903057905	23.15477481385094
49	21.96920407720668	26.22713800332538	28.338032241740763	23.465625677727175
50	21.752331381479074	27.109086965950986	28.25851225330731	22.880069399262634
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	1.0
10	2.0
11	3.0
12	4.0
13	4.5
14	5.0
15	9.0
16	13.0
17	11.5
18	10.0
19	21.0
20	32.0
21	44.5
22	57.0
23	73.0
24	89.0
25	114.5
26	140.0
27	202.0
28	264.0
29	309.0
30	354.0
31	418.0
32	482.0
33	583.5
34	685.0
35	746.0
36	807.0
37	855.5
38	904.0
39	913.0
40	922.0
41	919.0
42	916.0
43	945.5
44	975.0
45	1035.0
46	1095.0
47	1084.0
48	1073.0
49	1048.0
50	1023.0
51	995.5
52	968.0
53	876.0
54	784.0
55	721.5
56	659.0
57	577.0
58	495.0
59	418.0
60	341.0
61	295.5
62	250.0
63	208.0
64	166.0
65	138.0
66	110.0
67	88.0
68	66.0
69	56.5
70	47.0
71	39.5
72	32.0
73	28.5
74	25.0
75	19.5
76	14.0
77	11.5
78	9.0
79	7.0
80	5.0
81	6.0
82	7.0
83	3.5
84	0.0
85	0.5
86	1.0
87	0.5
88	0.0
89	0.5
90	1.0
91	1.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.02168726957276079
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.00722908985758693
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.00722908985758693
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.00722908985758693
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	13833.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	94.94686618954674
#Duplication Level	Percentage of deduplicated	Percentage of total
1	95.50784224151059	90.68170317357045
2	4.126694076442821	7.836333405624233
3	0.25887010811633926	0.7373671654738668
4	0.05329678696512867	0.20241451601243401
5	0.015227653418608192	0.07229089857586929
6	0.007613826709304096	0.04337453914552158
7	0.007613826709304096	0.050603629003108504
8	0.0	0.0
9	0.0	0.0
>10	0.02284148012791229	0.3759126725945203
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGT	24	0.1734981565820863	No Hit
ATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTT	17	0.12289452757897781	Illumina PCR Primer Index 10 (95% over 21bp)
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.00722908985758693	0.0
24	0.0	0.0	0.0	0.00722908985758693	0.0
25	0.0	0.0	0.0	0.00722908985758693	0.0
26	0.0	0.0	0.0	0.00722908985758693	0.0
27	0.0	0.0	0.0	0.00722908985758693	0.0
28	0.0	0.0	0.0	0.00722908985758693	0.0
29	0.0	0.0	0.0	0.01445817971517386	0.0
30	0.0	0.0	0.0	0.02168726957276079	0.0
31	0.0	0.0	0.0	0.05060362900310851	0.0
32	0.0	0.0	0.0	0.11566543772139087	0.0
33	0.0	0.0	0.0	0.1662690667244994	0.0
34	0.0	0.0	0.0	0.23133087544278175	0.0
35	0.0	0.0	0.0	0.3759126725945203	0.0
36	0.0	0.0	0.0	0.513265379888672	0.0
37	0.0	0.0	0.0	0.6433889973252368	0.0
38	0.0	0.0	0.0	0.932552591628714	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE