FastQCFastQC Report
Thu 2 Feb 2017
SRR4063876_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063876_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3501900
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT70090.20014849081926955No Hit
TATCAACGCAGAGTACTTTTTTTTT47180.1347268625603244No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA50250.012.0231481
GGTATCA19300.011.8619721
CGGCCGA600.005870194611.0853517
GCGTTAT2350.010.5099981
ACGGTAT4950.010.3643389
GTATTAG5350.010.2984381
CGAGCCG6000.010.29280515
GTATAGG2600.010.2301471
GCATCGC4750.010.20098214
TAAATCC5150.010.1429844
GTCCTAT6950.010.1144911
TTACCGC6750.09.85308216
CGGTTCT6750.09.85265912
TATAAGC1856.548362E-109.7530982
GTCCTAC3900.09.7429961
GCGTAAC2151.0913936E-119.72158811
CGTTATT2151.0913936E-119.7172842
GGTTCTA6850.09.70868713
CGAACGA2450.09.69508716
ACCGTCG5000.09.6906558
Produced by FastQC (version 0.11.5)