##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063876_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3501900
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.220479168451412	32.0	32.0	32.0	32.0	32.0
2	30.769545104086355	32.0	32.0	32.0	32.0	32.0
3	30.798277792055742	32.0	32.0	32.0	32.0	32.0
4	30.833055769725007	32.0	32.0	32.0	32.0	32.0
5	30.71090722179388	32.0	32.0	32.0	32.0	32.0
6	34.38221765327394	36.0	36.0	36.0	32.0	36.0
7	34.317783774522404	36.0	36.0	36.0	32.0	36.0
8	34.280104800251294	36.0	36.0	36.0	32.0	36.0
9	34.43588109312088	36.0	36.0	36.0	32.0	36.0
10	34.092434963876755	36.0	36.0	36.0	32.0	36.0
11	34.413254518975414	36.0	36.0	36.0	32.0	36.0
12	34.21119877780633	36.0	36.0	36.0	32.0	36.0
13	34.30060481452926	36.0	36.0	36.0	32.0	36.0
14	34.18788971701076	36.0	36.0	36.0	32.0	36.0
15	34.12291413232816	36.0	36.0	36.0	32.0	36.0
16	34.128267797481364	36.0	36.0	36.0	32.0	36.0
17	34.0426768325766	36.0	36.0	36.0	32.0	36.0
18	34.038018504240554	36.0	36.0	36.0	32.0	36.0
19	34.05904623204546	36.0	36.0	36.0	32.0	36.0
20	34.04319940603672	36.0	36.0	36.0	32.0	36.0
21	34.033277934835375	36.0	36.0	36.0	32.0	36.0
22	34.006290870670206	36.0	36.0	36.0	32.0	36.0
23	33.95036009023673	36.0	36.0	36.0	32.0	36.0
24	33.93545789428596	36.0	36.0	36.0	32.0	36.0
25	33.52041491761615	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	7.0
4	24.0
5	46.0
6	146.0
7	54.0
8	187.0
9	217.0
10	322.0
11	113.0
12	226.0
13	179.0
14	313.0
15	579.0
16	968.0
17	1300.0
18	1858.0
19	2471.0
20	3909.0
21	5997.0
22	9156.0
23	14053.0
24	21158.0
25	30265.0
26	42723.0
27	55479.0
28	74842.0
29	99048.0
30	129663.0
31	174148.0
32	242652.0
33	339178.0
34	713722.0
35	1536897.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.167925349751634	17.320376267967514	11.67758515679546	25.83411322548539
2	16.877526274396022	19.788514029479412	37.19201370306634	26.141945993058226
3	18.56979310608728	24.20888086644661	28.445335784613988	28.775990242852124
4	12.967457491013878	15.66256562003022	35.47229716010874	35.89767972884717
5	15.078237471452196	36.26656857769757	33.08780628647101	15.567387664379225
6	33.93701846583647	34.88722779405838	17.322192390043064	13.853561350062074
7	30.551110933389698	30.189739825369667	20.940273227633522	18.318876013607113
8	28.463938667583065	32.16826765002381	19.414961917192212	19.952831765200916
9	27.59932151925062	14.522882154682067	18.52077025907005	39.35702606699726
10	16.4059479804576	26.34944028032985	30.83148350642037	26.413128232792182
11	36.67724012688152	21.120910593784266	22.81144516591342	19.39040411342079
12	25.389398441520445	23.165003330292112	28.40311993080536	23.042478297382086
13	29.283356907119284	19.505293386481508	25.35004929064335	25.86130041575586
14	24.181870812126967	19.379409687961758	25.057500430771025	31.381219069140247
15	25.333984038484562	26.83610144942858	22.124994249659906	25.70492026242695
16	25.966089567524257	25.49900387818853	23.33639656506567	25.19850998922154
17	24.349159133802807	25.780724726509536	24.867180144385248	25.002935995302405
18	25.003693732029674	24.824422698976413	25.762644923611905	24.409238645382008
19	25.867185783128466	24.669081830857156	25.097484367493987	24.366248018520388
20	26.202883490917074	23.999911977164363	24.60129656493742	25.195907966981146
21	27.05845437741979	23.85750652420672	24.229040521525025	24.85499857684847
22	26.366340139468274	23.7256965769086	24.551897933982197	25.35606534964093
23	24.76425481371736	23.830533827987395	25.231929822455545	26.1732815358397
24	25.04555969770708	24.61261039849911	24.85797691222846	25.48385299156535
25	25.21347284503096	24.13138364468928	24.92603218432325	25.72911132595651
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	235.0
1	235.0
2	652.5
3	1070.0
4	1070.0
5	1070.0
6	2530.5
7	3991.0
8	3991.0
9	3991.0
10	4369.5
11	4748.0
12	4748.0
13	4748.0
14	5992.5
15	7237.0
16	7237.0
17	7237.0
18	13103.0
19	18969.0
20	18969.0
21	18969.0
22	31430.5
23	43892.0
24	43892.0
25	43892.0
26	65811.5
27	87731.0
28	87731.0
29	87731.0
30	112147.0
31	136563.0
32	136563.0
33	136563.0
34	174113.0
35	211663.0
36	211663.0
37	211663.0
38	248480.0
39	285297.0
40	285297.0
41	285297.0
42	327504.0
43	369711.0
44	369711.0
45	369711.0
46	414023.5
47	458336.0
48	458336.0
49	458336.0
50	471293.5
51	484251.0
52	484251.0
53	484251.0
54	454985.5
55	425720.0
56	425720.0
57	425720.0
58	390601.0
59	355482.0
60	355482.0
61	355482.0
62	310665.5
63	265849.0
64	265849.0
65	265849.0
66	215674.0
67	165499.0
68	165499.0
69	165499.0
70	125569.5
71	85640.0
72	85640.0
73	85640.0
74	64569.0
75	43498.0
76	43498.0
77	43498.0
78	34826.0
79	26154.0
80	26154.0
81	26154.0
82	17948.0
83	9742.0
84	9742.0
85	9742.0
86	7493.5
87	5245.0
88	5245.0
89	5245.0
90	3891.5
91	2538.0
92	2538.0
93	2538.0
94	1761.0
95	984.0
96	984.0
97	984.0
98	1419.5
99	1855.0
100	1855.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04043519232416688
2	0.005768297210085953
3	0.0016276878266084124
4	0.0031697078728690135
5	0.009052228790085382
6	0.013164282246780318
7	0.022559182158256946
8	0.03538079328364602
9	0.04931608555355664
10	0.05776863988120734
11	0.05545560981181644
12	0.06302293040920642
13	0.0642508352608584
14	0.0674205431337274
15	0.060167337730946055
16	0.06647819754990149
17	0.06382249635911934
18	0.07792912418972558
19	0.07484508409720438
20	0.08018504240555127
21	0.07493075187755219
22	0.07861446643250806
23	0.08249807247494217
24	0.07553042633998687
25	0.07832890716468202
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	3501900.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.44914577469265
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.71268338183944	31.593039799266144
2	14.021808384925738	12.184711930828923
3	4.996549595160527	6.512874351918339
4	2.463343410662237	4.281206677719685
5	1.3491081663547309	2.9308798692887503
6	0.8536475913663448	2.2254155182494952
7	0.5995947899017812	1.8236317002532094
8	0.44126491551761093	1.5338066911665693
9	0.327189402242234	1.2794490030561636
>10	2.002590851942008	16.00597384735186
>50	0.13585869155228433	4.042436276198625
>100	0.07744341392299942	6.8050109146318105
>500	0.011906058848686396	3.5706328488087227
>1k	0.006945200992061657	5.0094965503262285
>5k	6.614477135296816E-5	0.20143402093556537
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	7009	0.20014849081926955	No Hit
TATCAACGCAGAGTACTTTTTTTTT	4718	0.1347268625603244	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.4277963391301864E-4
2	0.0	0.0	0.0	0.0	1.4277963391301864E-4
3	0.0	0.0	0.0	0.0	1.4277963391301864E-4
4	0.0	0.0	0.0	0.0	1.4277963391301864E-4
5	2.8555926782603728E-5	0.0	0.0	0.0	1.713355606956224E-4
6	1.998914874782261E-4	0.0	0.0	0.0	2.2844741426082983E-4
7	1.998914874782261E-4	0.0	0.0	0.0	2.2844741426082983E-4
8	1.998914874782261E-4	0.0	0.0	0.0	2.5700334104343357E-4
9	1.998914874782261E-4	0.0	0.0	0.0	2.5700334104343357E-4
10	1.998914874782261E-4	0.0	0.0	0.0	2.5700334104343357E-4
11	1.998914874782261E-4	0.0	0.0	0.0	2.5700334104343357E-4
12	1.998914874782261E-4	0.0	0.0	0.0	3.712270481738485E-4
13	1.998914874782261E-4	0.0	0.0	0.0	3.712270481738485E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	5025	0.0	12.023148	1
GGTATCA	1930	0.0	11.861972	1
CGGCCGA	60	0.0058701946	11.08535	17
GCGTTAT	235	0.0	10.509998	1
ACGGTAT	495	0.0	10.364338	9
GTATTAG	535	0.0	10.298438	1
CGAGCCG	600	0.0	10.292805	15
GTATAGG	260	0.0	10.230147	1
GCATCGC	475	0.0	10.200982	14
TAAATCC	515	0.0	10.142984	4
GTCCTAT	695	0.0	10.114491	1
TTACCGC	675	0.0	9.853082	16
CGGTTCT	675	0.0	9.852659	12
TATAAGC	185	6.548362E-10	9.753098	2
GTCCTAC	390	0.0	9.742996	1
GCGTAAC	215	1.0913936E-11	9.721588	11
CGTTATT	215	1.0913936E-11	9.717284	2
GGTTCTA	685	0.0	9.708687	13
CGAACGA	245	0.0	9.695087	16
ACCGTCG	500	0.0	9.690655	8
>>END_MODULE