FastQCFastQC Report
Thu 2 Feb 2017
SRR4063876_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063876_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3501900
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA55850.023.5961951
GGTATCA26350.020.0386711
AACGCAG78550.016.747266
AATAGCG8350.016.07065
TCAACGC82650.015.8366324
TAGCGTA8500.015.7870017
TATCAAC84750.015.6523212
ATCAACG83500.015.64933
CAACGCA84250.015.5619895
GTATAGG4600.015.3048981
CGTATAT8500.015.27004810
ATAGCGT8500.015.2693946
GCATTCG12850.015.06582922
TAGGACG3100.014.902064
GTACATG128150.014.6271361
AGCGTAT9100.014.5043598
TAGACAG6100.014.4251015
TACATGG130450.014.3511462
ACCGTCG10500.014.2465068
ACATGGG127750.014.1720883
Produced by FastQC (version 0.11.5)