##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063876_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3501900
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.34990576544162	32.0	32.0	32.0	32.0	32.0
2	31.41071703932151	32.0	32.0	32.0	32.0	32.0
3	31.485052685684913	32.0	32.0	32.0	32.0	32.0
4	31.569394899911476	32.0	32.0	32.0	32.0	32.0
5	31.52204631771324	32.0	32.0	32.0	32.0	32.0
6	35.1117173534367	36.0	36.0	36.0	36.0	36.0
7	35.12283588908878	36.0	36.0	36.0	36.0	36.0
8	35.07105656929096	36.0	36.0	36.0	36.0	36.0
9	35.174834518404296	36.0	36.0	36.0	36.0	36.0
10	35.04008395442474	36.0	36.0	36.0	36.0	36.0
11	35.175532425254865	36.0	36.0	36.0	36.0	36.0
12	35.08569262400411	36.0	36.0	36.0	36.0	36.0
13	35.12684028670151	36.0	36.0	36.0	36.0	36.0
14	35.068892886718636	36.0	36.0	36.0	36.0	36.0
15	35.04273622890431	36.0	36.0	36.0	36.0	36.0
16	35.05729432593735	36.0	36.0	36.0	36.0	36.0
17	35.01981067420543	36.0	36.0	36.0	36.0	36.0
18	35.01313001513464	36.0	36.0	36.0	36.0	36.0
19	35.01516805162912	36.0	36.0	36.0	36.0	36.0
20	35.006633256232334	36.0	36.0	36.0	36.0	36.0
21	35.00487935120935	36.0	36.0	36.0	36.0	36.0
22	34.986979354064935	36.0	36.0	36.0	36.0	36.0
23	34.93521574002684	36.0	36.0	36.0	36.0	36.0
24	34.91105142922414	36.0	36.0	36.0	32.0	36.0
25	34.88384162883006	36.0	36.0	36.0	32.0	36.0
26	34.823112881578574	36.0	36.0	36.0	32.0	36.0
27	34.82173305919643	36.0	36.0	36.0	32.0	36.0
28	34.79788486250321	36.0	36.0	36.0	32.0	36.0
29	34.771753619463716	36.0	36.0	36.0	32.0	36.0
30	34.75738513378452	36.0	36.0	36.0	32.0	36.0
31	34.743432422399266	36.0	36.0	36.0	32.0	36.0
32	34.71242639709872	36.0	36.0	36.0	32.0	36.0
33	34.69223535794854	36.0	36.0	36.0	32.0	36.0
34	34.682574031240186	36.0	36.0	36.0	32.0	36.0
35	34.64102030326394	36.0	36.0	36.0	32.0	36.0
36	34.62558325480453	36.0	36.0	36.0	32.0	36.0
37	34.61944715725749	36.0	36.0	36.0	32.0	36.0
38	34.580740740740744	36.0	36.0	36.0	32.0	36.0
39	34.586402524343924	36.0	36.0	36.0	32.0	36.0
40	34.56151917530484	36.0	36.0	36.0	32.0	36.0
41	34.54795025557554	36.0	36.0	36.0	32.0	36.0
42	34.47715497301465	36.0	36.0	36.0	32.0	36.0
43	34.4721017162112	36.0	36.0	36.0	32.0	36.0
44	34.42201090836403	36.0	36.0	36.0	32.0	36.0
45	34.387084439875494	36.0	36.0	36.0	32.0	36.0
46	34.37497187241212	36.0	36.0	36.0	32.0	36.0
47	34.34543819069648	36.0	36.0	36.0	32.0	36.0
48	34.30348467974528	36.0	36.0	36.0	32.0	36.0
49	34.293087181244466	36.0	36.0	36.0	32.0	36.0
50	33.69624089779834	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	2.0
18	1.0
19	15.0
20	42.0
21	148.0
22	427.0
23	1083.0
24	2844.0
25	6422.0
26	12935.0
27	23535.0
28	39002.0
29	59489.0
30	85674.0
31	121369.0
32	176324.0
33	284219.0
34	596023.0
35	2092345.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.363093436175234	18.184458566420187	12.185525520654245	26.266922476750338
2	16.133972455969413	20.554735679422848	36.66968980126126	26.641602063346475
3	17.811340000405494	24.837123527972203	28.688075885943775	28.663460585678525
4	12.645335389360064	16.33924441017733	35.44638624746566	35.56903395299694
5	14.702704246266313	36.640652217367716	32.876210057397415	15.780433478968561
6	34.04849891187423	35.21840901478143	17.05032106920457	13.68277100413976
7	30.512359151420416	30.368751354622166	20.857197766126323	18.2616917278311
8	28.55653461164928	32.35932846721165	19.237048241539807	19.84708867959927
9	27.73734216977016	14.33039661163312	18.354824744764503	39.57743647383222
10	16.298928969788072	26.5284011762885	30.860696395232022	26.311973458691405
11	36.9578228961421	21.028441703075472	22.690682201090837	19.323053199691596
12	25.3276510781588	23.249862360275692	28.32668360225432	23.095802959311186
13	29.423872484530456	19.48007370895813	25.323561822917455	25.77249198359396
14	24.20348080309707	19.304102095637987	25.015612984177416	31.476804117087532
15	25.38855738655267	26.926395058449597	22.04978389284169	25.635263662156042
16	26.065942940298285	25.523690730339965	23.22826393624446	25.18210239311729
17	24.425911647962533	25.843884748279507	24.77240926354265	24.95779434021531
18	25.18109491066689	24.74321745410268	25.69373193298596	24.381955702244472
19	25.951726749322795	24.683557316632296	25.040877832535386	24.323838101509523
20	26.40513689349416	23.84697032996248	24.577834268020947	25.170058508522413
21	27.148033924441016	23.86124675176333	24.16650960906936	24.82420971472629
22	26.397029721630638	23.795551778551836	24.569504388196787	25.23791411162074
23	24.80466173254663	23.74951240393001	25.20741751416527	26.23840834935809
24	25.210857926761708	24.56046516675024	24.816955673003445	25.411721233484602
25	25.385541053939765	24.071942914451636	24.93711862819233	25.60539740341627
26	25.174692173670678	24.728312940085917	25.220869335765656	24.87612555047775
27	25.630555196997097	24.456831528673696	24.464285102333854	25.448328171995353
28	25.121068313999146	24.220887775284417	25.21153757187137	25.446506338845065
29	25.059098211832893	24.47439858038642	25.109529447443858	25.35697376033683
30	25.00932387752223	24.639167367742296	25.170328537462	25.181180217273475
31	25.616781365106583	24.272870530030737	24.472862191095274	25.63748591376741
32	25.395654115773738	24.462418713140345	24.21597374604346	25.925953425042465
33	24.956143169029065	24.189694537136592	24.903397887047422	25.95076440678692
34	25.684647895368094	24.179764692443886	24.96010623108116	25.175481181106857
35	26.011779740274577	24.16523049025137	24.95885657987735	24.864133189596703
36	25.277708206546308	24.63417510874293	24.58576876274236	25.5023479219684
37	25.99830200204411	24.395113215570063	24.545351480722182	25.061233301663645
38	25.206853369925764	24.411510622894735	24.896369838661084	25.48526616851842
39	25.8246931468974	24.24991225833203	24.405891099810752	25.519503494959817
40	25.906381530231947	24.509846196637223	24.735101200550915	24.848671072579915
41	25.102983016405034	24.75290501346311	25.017312569482304	25.126799400649553
42	26.148481940922487	24.784855961437415	24.444549151303317	24.622112946336784
43	25.51317278163892	24.035950222768474	24.829475458915624	25.621401536676984
44	25.321936700369776	24.49977197786429	24.42992324349423	25.748368078271707
45	25.387645655361673	24.673564935055666	24.49254607214197	25.446243337440695
46	25.287219120375028	24.45857282538174	24.632966061425712	25.621241992817524
47	25.417589638311245	24.465114313316235	24.94115207993094	25.17614396844158
48	26.02424157620219	24.931899989576895	24.015351951191306	25.02850648302961
49	25.369336800037694	25.01230051814951	24.144110957384378	25.474251724428417
50	25.41941972584871	25.151362332382032	24.15871899006871	25.270498951700553
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	27.0
1	52.5
2	78.0
3	199.5
4	321.0
5	394.0
6	467.0
7	462.0
8	457.0
9	583.5
10	710.0
11	1107.5
12	1505.0
13	2782.5
14	4060.0
15	5353.5
16	6647.0
17	6694.5
18	6742.0
19	6646.0
20	6550.0
21	7248.0
22	7946.0
23	9438.5
24	10931.0
25	12855.5
26	14780.0
27	18900.0
28	23020.0
29	28409.0
30	33798.0
31	40081.5
32	46365.0
33	54946.5
34	63528.0
35	74697.0
36	85866.0
37	101701.0
38	117536.0
39	132059.5
40	146583.0
41	161971.5
42	177360.0
43	184296.5
44	191233.0
45	210360.0
46	229487.0
47	246510.0
48	263533.0
49	273737.5
50	283942.0
51	277512.0
52	271082.0
53	269618.5
54	268155.0
55	267846.0
56	267537.0
57	258866.0
58	250195.0
59	229906.5
60	209618.0
61	183404.0
62	157190.0
63	135424.5
64	113659.0
65	96026.0
66	78393.0
67	66511.0
68	54629.0
69	48583.5
70	42538.0
71	32973.5
72	23409.0
73	19703.5
74	15998.0
75	12460.0
76	8922.0
77	7704.0
78	6486.0
79	5401.0
80	4316.0
81	3455.5
82	2595.0
83	2210.5
84	1826.0
85	1335.0
86	844.0
87	611.0
88	378.0
89	301.0
90	224.0
91	181.0
92	138.0
93	108.0
94	78.0
95	91.0
96	104.0
97	85.0
98	66.0
99	57.0
100	48.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009823238813215684
2	4.5689482852165965E-4
3	3.712270481738485E-4
4	0.0
5	0.0
6	1.998914874782261E-4
7	2.8555926782603728E-5
8	2.8555926782603728E-5
9	0.001028013364173734
10	0.0010851252177389416
11	0.0
12	1.1422370713041491E-4
13	5.425626088694708E-4
14	1.998914874782261E-4
15	0.004711727919129616
16	6.853422427824896E-4
17	0.0
18	1.998914874782261E-4
19	5.7111853565207456E-5
20	4.854507553042634E-4
21	0.0
22	1.713355606956224E-4
23	7.42454096347697E-4
24	4.5689482852165965E-4
25	0.0019418030212170537
26	0.00482595162626003
27	0.0063965275993032355
28	0.004511836431651389
29	0.0029127045318255804
30	0.0039407178959993145
31	0.007253205402781348
32	0.002969816385390788
33	0.004711727919129616
34	0.002855592678260373
35	0.003569490847825466
36	0.008366886547302893
37	0.003683714554955881
38	0.007567320597389989
39	0.004083497529912333
40	0.0025985893372169394
41	0.003112596019303807
42	0.0017133556069562237
43	0.0016562437533910162
44	0.0013421285587823751
45	0.001570575973043205
46	0.001256460778434564
47	0.0021988063622604874
48	0.0018561352408692426
49	4.2833890173905593E-4
50	0.0010851252177389416
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	3501900.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.20619859316336
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.57932929037673	33.99821101542909
2	14.025128746828367	12.960957683412715
3	4.8564743980669585	6.73197661499086
4	2.19808215947054	4.062600831383406
5	1.236704707964998	2.8571711668665394
6	0.8175939678696428	2.2666745548774276
7	0.559761991167851	1.810513161016439
8	0.3990727122157552	1.4751706395002808
9	0.29979277691421274	1.246705612820463
>10	1.8302016113193955	15.511784005129165
>50	0.11454195665708274	3.610679551861696
>100	0.0683573212794029	6.346931083955543
>500	0.00959828091855502	3.0771705473028947
>1k	0.00536007895071081	4.043453531453429
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	2.8555926782603728E-5
6	0.0	0.0	0.0	0.0	1.4277963391301864E-4
7	0.0	0.0	0.0	0.0	1.4277963391301864E-4
8	0.0	0.0	0.0	2.8555926782603728E-5	1.4277963391301864E-4
9	0.0	0.0	0.0	2.8555926782603728E-5	1.4277963391301864E-4
10	0.0	0.0	0.0	2.8555926782603728E-5	1.713355606956224E-4
11	0.0	0.0	0.0	2.8555926782603728E-5	1.713355606956224E-4
12	0.0	0.0	0.0	2.8555926782603728E-5	3.14115194608641E-4
13	0.0	0.0	0.0	2.8555926782603728E-5	3.712270481738485E-4
14	2.8555926782603728E-5	0.0	0.0	2.8555926782603728E-5	3.997829749564522E-4
15	2.8555926782603728E-5	0.0	0.0	2.8555926782603728E-5	5.140066820868671E-4
16	2.8555926782603728E-5	0.0	0.0	2.8555926782603728E-5	5.425626088694708E-4
17	2.8555926782603728E-5	0.0	0.0	2.8555926782603728E-5	5.425626088694708E-4
18	2.8555926782603728E-5	0.0	0.0	2.8555926782603728E-5	5.996744624346784E-4
19	2.8555926782603728E-5	0.0	0.0	2.8555926782603728E-5	5.996744624346784E-4
20	2.8555926782603728E-5	0.0	0.0	5.7111853565207456E-5	7.710100231303007E-4
21	2.8555926782603728E-5	0.0	0.0	5.7111853565207456E-5	9.137896570433193E-4
22	2.8555926782603728E-5	0.0	0.0	5.7111853565207456E-5	9.423455838259231E-4
23	5.7111853565207456E-5	0.0	0.0	1.4277963391301864E-4	9.423455838259231E-4
24	5.7111853565207456E-5	0.0	0.0	3.426711213912448E-4	9.423455838259231E-4
25	5.7111853565207456E-5	2.8555926782603728E-5	0.0	4.5689482852165965E-4	9.423455838259231E-4
26	5.7111853565207456E-5	2.8555926782603728E-5	0.0	5.140066820868671E-4	9.709015106085268E-4
27	5.7111853565207456E-5	2.8555926782603728E-5	0.0	6.853422427824896E-4	9.994574373911305E-4
28	5.7111853565207456E-5	2.8555926782603728E-5	0.0	0.0010851252177389416	9.994574373911305E-4
29	5.7111853565207456E-5	2.8555926782603728E-5	0.0	0.0018561352408692424	9.994574373911305E-4
30	5.7111853565207456E-5	2.8555926782603728E-5	0.0	0.003997829749564522	9.994574373911305E-4
31	5.7111853565207456E-5	2.8555926782603728E-5	0.0	0.009566235472172249	0.0010280133641737343
32	5.7111853565207456E-5	2.8555926782603728E-5	0.0	0.01716211199634484	0.001056569290956338
33	5.7111853565207456E-5	2.8555926782603728E-5	0.0	0.024500985179474	0.001056569290956338
34	5.7111853565207456E-5	5.7111853565207456E-5	0.0	0.033210542848168136	0.0011422370713041492
35	5.7111853565207456E-5	5.7111853565207456E-5	0.0	0.0428624461006882	0.0011422370713041492
36	5.7111853565207456E-5	5.7111853565207456E-5	0.0	0.058082755075815985	0.0011422370713041492
37	5.7111853565207456E-5	8.56677803478112E-5	0.0	0.0812416116965076	0.0011422370713041492
38	5.7111853565207456E-5	8.56677803478112E-5	0.0	0.11448071047145836	0.001170792998086753
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	5585	0.0	23.596195	1
GGTATCA	2635	0.0	20.038671	1
AACGCAG	7855	0.0	16.74726	6
AATAGCG	835	0.0	16.0706	5
TCAACGC	8265	0.0	15.836632	4
TAGCGTA	850	0.0	15.787001	7
TATCAAC	8475	0.0	15.652321	2
ATCAACG	8350	0.0	15.6493	3
CAACGCA	8425	0.0	15.561989	5
GTATAGG	460	0.0	15.304898	1
CGTATAT	850	0.0	15.270048	10
ATAGCGT	850	0.0	15.269394	6
GCATTCG	1285	0.0	15.065829	22
TAGGACG	310	0.0	14.90206	4
GTACATG	12815	0.0	14.627136	1
AGCGTAT	910	0.0	14.504359	8
TAGACAG	610	0.0	14.425101	5
TACATGG	13045	0.0	14.351146	2
ACCGTCG	1050	0.0	14.246506	8
ACATGGG	12775	0.0	14.172088	3
>>END_MODULE