FastQCFastQC Report
Thu 2 Feb 2017
SRR4063875_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063875_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2187718
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT32600.14901372114687542No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA7050.014.4126611
GCGTTAT801.295557E-714.2442891
TCCGTTG500.001498731313.30136113
GTATCAA22050.013.1353231
TCGCCAG751.4769834E-512.66854317
CGCGTAA604.0912553E-412.66738310
GTACCGA802.867292E-511.8751296
TCGCGTA658.021902E-411.6929699
CTTATAC1657.2759576E-1211.5113263
GCGAAAT600.005871478511.08472112
TAATAGT1303.274181E-810.9601534
TACCGAT700.001491118310.8577577
GCGTAAC803.7684303E-410.68859411
TCGTGCG909.50614E-510.55687812
GTCCTAA4450.010.4564821
GTAGGAC6100.010.2752743
TAGGACC4350.010.263074
ACTGTCG1303.838777E-710.2315828
CGCATCG856.589026E-410.05985313
GCGGTCG951.6435781E-410.0005669
Produced by FastQC (version 0.11.5)