##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063875_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2187718
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.334039396302447	32.0	32.0	32.0	32.0	32.0
2	30.98438829867469	32.0	32.0	32.0	32.0	32.0
3	30.991159738138098	32.0	32.0	32.0	32.0	32.0
4	31.022085113346417	32.0	32.0	32.0	32.0	32.0
5	30.962121717698533	32.0	32.0	32.0	32.0	32.0
6	34.63622139599345	36.0	36.0	36.0	32.0	36.0
7	34.58631322684185	36.0	36.0	36.0	32.0	36.0
8	34.56573790589098	36.0	36.0	36.0	32.0	36.0
9	34.666887139933024	36.0	36.0	36.0	32.0	36.0
10	34.420225092996446	36.0	36.0	36.0	32.0	36.0
11	34.632666093161916	36.0	36.0	36.0	32.0	36.0
12	34.491943660014684	36.0	36.0	36.0	32.0	36.0
13	34.53552880215823	36.0	36.0	36.0	32.0	36.0
14	34.458788107059505	36.0	36.0	36.0	32.0	36.0
15	34.414200550527994	36.0	36.0	36.0	32.0	36.0
16	34.40560712121032	36.0	36.0	36.0	32.0	36.0
17	34.35813253810592	36.0	36.0	36.0	32.0	36.0
18	34.34363524000808	36.0	36.0	36.0	32.0	36.0
19	34.35961810434434	36.0	36.0	36.0	32.0	36.0
20	34.33460573986227	36.0	36.0	36.0	32.0	36.0
21	34.32764689050417	36.0	36.0	36.0	32.0	36.0
22	34.30342850403937	36.0	36.0	36.0	32.0	36.0
23	34.242283054763	36.0	36.0	36.0	32.0	36.0
24	34.250418472581934	36.0	36.0	36.0	32.0	36.0
25	33.86084221092481	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	8.0
4	21.0
5	32.0
6	94.0
7	39.0
8	137.0
9	143.0
10	194.0
11	63.0
12	120.0
13	104.0
14	162.0
15	306.0
16	546.0
17	720.0
18	876.0
19	1259.0
20	1820.0
21	2601.0
22	4111.0
23	6511.0
24	9593.0
25	14309.0
26	20830.0
27	27773.0
28	38314.0
29	51813.0
30	69073.0
31	94425.0
32	134633.0
33	193629.0
34	433373.0
35	1080086.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.560431344448624	17.255332701980073	11.883601749924663	26.300634203646638
2	16.298653686864718	19.732319012033255	38.2317366397209	25.73729066138113
3	18.932905116749357	23.28985344278271	28.91942964183943	28.857811798628504
4	13.128923199008241	16.141455494261365	35.675169635788166	35.05445167094223
5	14.781976644518044	36.185545566961295	34.07184930708413	14.96062848143653
6	33.78019566608759	35.16672762183414	17.390554996799853	13.662521715278414
7	29.97195472887895	30.672675017762614	20.756518216624347	18.598852036734094
8	27.373088520008526	33.911239470341364	19.57114990136804	19.144522108282068
9	27.355103780513794	14.973607837434159	18.374268396082748	39.2970199859693
10	15.724491522710537	27.41674784923368	32.36197820190905	24.496782426146734
11	36.31385943264111	21.416275093963726	23.027036124697116	19.242829348698045
12	24.739694347831655	23.50060102201186	29.37442482101985	22.385279809136634
13	29.542879594634343	20.301718952756588	24.98443698476814	25.170964467840935
14	23.30574391931297	19.88116502189848	25.677000308751385	31.136090750037166
15	24.92902661610523	27.399499538735895	22.56379600463414	25.10767784052474
16	25.160217047157857	26.070633179234058	24.247102233821977	24.52204753978611
17	23.78094352067079	26.254870051163554	25.48961223461137	24.47457419355429
18	23.976613269924513	26.006003588246436	26.37484641079292	23.642536731036135
19	25.324804239174487	25.329515856192298	25.44538503974684	23.900294864886376
20	25.573800489022165	25.256736634804746	25.25971010912651	23.909752767046584
21	25.8708850475652	24.634089216541966	25.03284459839016	24.462181137502675
22	25.357989958943744	25.10254914742512	25.315584222504317	24.223876671126817
23	24.69740180483846	25.080218600833888	25.26019007256501	24.96218952176264
24	24.40634392025727	25.34137225930111	25.70591437445964	24.546369445981988
25	24.786437781276792	25.109079813761486	25.39700111710172	24.707481287860006
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	75.0
1	75.0
2	245.5
3	416.0
4	416.0
5	416.0
6	873.0
7	1330.0
8	1330.0
9	1330.0
10	1971.5
11	2613.0
12	2613.0
13	2613.0
14	4198.5
15	5784.0
16	5784.0
17	5784.0
18	10366.0
19	14948.0
20	14948.0
21	14948.0
22	23215.5
23	31483.0
24	31483.0
25	31483.0
26	46608.0
27	61733.0
28	61733.0
29	61733.0
30	83410.5
31	105088.0
32	105088.0
33	105088.0
34	126139.0
35	147190.0
36	147190.0
37	147190.0
38	171467.0
39	195744.0
40	195744.0
41	195744.0
42	220439.0
43	245134.0
44	245134.0
45	245134.0
46	264734.0
47	284334.0
48	284334.0
49	284334.0
50	289366.5
51	294399.0
52	294399.0
53	294399.0
54	278172.5
55	261946.0
56	261946.0
57	261946.0
58	235056.5
59	208167.0
60	208167.0
61	208167.0
62	177896.0
63	147625.0
64	147625.0
65	147625.0
66	120295.0
67	92965.0
68	92965.0
69	92965.0
70	68892.0
71	44819.0
72	44819.0
73	44819.0
74	33246.5
75	21674.0
76	21674.0
77	21674.0
78	16655.5
79	11637.0
80	11637.0
81	11637.0
82	8047.0
83	4457.0
84	4457.0
85	4457.0
86	3343.0
87	2229.0
88	2229.0
89	2229.0
90	1494.0
91	759.0
92	759.0
93	759.0
94	532.0
95	305.0
96	305.0
97	305.0
98	584.5
99	864.0
100	864.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04027027249398688
2	0.005210909267099325
3	0.0018283892165260787
4	0.004113875737183677
5	0.010193269882132889
6	0.014535694271382326
7	0.024683254423102065
8	0.036567784330521574
9	0.04954934776785673
10	0.05887413277213974
11	0.05649722679065583
12	0.06591343125576514
13	0.0660505604470046
14	0.0682446275068359
15	0.06102249010155788
16	0.06696475505526764
17	0.06381078365676016
18	0.07734086385905313
19	0.07459828003426401
20	0.07875786550186084
21	0.07615241086831119
22	0.07738657358946628
23	0.08300887043028397
24	0.07715802493740052
25	0.07788938062401095
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2187718.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.121080069499246
#Duplication Level	Percentage of deduplicated	Percentage of total
1	54.357127066205	16.916525037753104
2	15.490251245349478	9.64146698606363
3	8.352530783130971	7.798193378543286
4	5.377683665362234	6.694372957527051
5	3.5922719909216303	5.589769213044576
6	2.594877029275082	4.845322547914449
7	1.9318682495617732	4.20852785348347
8	1.4435443305705986	3.5939726956447426
9	1.1160164071624756	3.125847236956045
>10	5.4949161322341	28.386372055335222
>50	0.16373759175350766	3.483695492003363
>100	0.08060892334563093	4.58821274143046
>500	0.004124657534545014	0.8376196221951016
>1k	4.4192759298696525E-4	0.29010218210555516
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3260	0.14901372114687542	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.570973041315197E-5
2	0.0	0.0	0.0	0.0	4.570973041315197E-5
3	0.0	0.0	0.0	0.0	4.570973041315197E-5
4	0.0	0.0	0.0	0.0	4.570973041315197E-5
5	0.0	0.0	0.0	0.0	4.570973041315197E-5
6	0.0	0.0	0.0	0.0	4.570973041315197E-5
7	0.0	0.0	0.0	0.0	4.570973041315197E-5
8	0.0	0.0	0.0	0.0	4.570973041315197E-5
9	0.0	0.0	0.0	4.570973041315197E-5	4.570973041315197E-5
10	0.0	0.0	0.0	4.570973041315197E-5	4.570973041315197E-5
11	0.0	0.0	0.0	4.570973041315197E-5	4.570973041315197E-5
12	0.0	0.0	0.0	4.570973041315197E-5	2.742583824789118E-4
13	0.0	0.0	0.0	4.570973041315197E-5	3.1996811289206376E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	705	0.0	14.412661	1
GCGTTAT	80	1.295557E-7	14.244289	1
TCCGTTG	50	0.0014987313	13.301361	13
GTATCAA	2205	0.0	13.135323	1
TCGCCAG	75	1.4769834E-5	12.668543	17
CGCGTAA	60	4.0912553E-4	12.667383	10
GTACCGA	80	2.867292E-5	11.875129	6
TCGCGTA	65	8.021902E-4	11.692969	9
CTTATAC	165	7.2759576E-12	11.511326	3
GCGAAAT	60	0.0058714785	11.084721	12
TAATAGT	130	3.274181E-8	10.960153	4
TACCGAT	70	0.0014911183	10.857757	7
GCGTAAC	80	3.7684303E-4	10.688594	11
TCGTGCG	90	9.50614E-5	10.556878	12
GTCCTAA	445	0.0	10.456482	1
GTAGGAC	610	0.0	10.275274	3
TAGGACC	435	0.0	10.26307	4
ACTGTCG	130	3.838777E-7	10.231582	8
CGCATCG	85	6.589026E-4	10.059853	13
GCGGTCG	95	1.6435781E-4	10.000566	9
>>END_MODULE