FastQCFastQC Report
Thu 2 Feb 2017
SRR4063875_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063875_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2187718
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA21900.029.8321591
GGTATCA7800.023.4075451
TATCAAC33350.019.391172
ATCAACG33500.019.1730213
TCAACGC33800.019.0032834
CAACGCA34600.018.56395
TAGACGC851.433025E-418.1151334
AACGCAG35850.018.0393376
CGCGGAT902.2179789E-417.1095242
ACGATAT1053.7773883E-516.76149615
CGTGATT3300.016.66778428
TAGGACG6650.016.539064
TCCCGAT800.001991192816.49733517
GTATAAT2301.8189894E-1216.2589871
GTATAAG2850.016.2086471
GTAGGAC9500.015.7448243
TAGGACC8750.015.586414
ACGCAGA41600.015.4930447
GTCTTAT2455.456968E-1215.2635381
GTATTAC2355.0931703E-1114.9769881
Produced by FastQC (version 0.11.5)