##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063875_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2187718
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.35659714826134	32.0	32.0	32.0	32.0	32.0
2	31.460205565799615	32.0	32.0	32.0	32.0	32.0
3	31.515264307374167	32.0	32.0	32.0	32.0	32.0
4	31.58456162997242	32.0	32.0	32.0	32.0	32.0
5	31.563185474544706	32.0	32.0	32.0	32.0	32.0
6	35.15251417230191	36.0	36.0	36.0	36.0	36.0
7	35.167337380777596	36.0	36.0	36.0	36.0	36.0
8	35.11196827013354	36.0	36.0	36.0	36.0	36.0
9	35.21103314046874	36.0	36.0	36.0	36.0	36.0
10	35.0861971241266	36.0	36.0	36.0	36.0	36.0
11	35.20149443392613	36.0	36.0	36.0	36.0	36.0
12	35.12854124708943	36.0	36.0	36.0	36.0	36.0
13	35.15467898513428	36.0	36.0	36.0	36.0	36.0
14	35.10909404228516	36.0	36.0	36.0	36.0	36.0
15	35.085366578324994	36.0	36.0	36.0	36.0	36.0
16	35.10129413388746	36.0	36.0	36.0	36.0	36.0
17	35.07285308252709	36.0	36.0	36.0	36.0	36.0
18	35.072188920144185	36.0	36.0	36.0	36.0	36.0
19	35.06020200044064	36.0	36.0	36.0	36.0	36.0
20	35.05479271094355	36.0	36.0	36.0	36.0	36.0
21	35.053648596391305	36.0	36.0	36.0	36.0	36.0
22	35.037986614362545	36.0	36.0	36.0	36.0	36.0
23	34.99073738022908	36.0	36.0	36.0	36.0	36.0
24	34.967762755528824	36.0	36.0	36.0	36.0	36.0
25	34.940515642326844	36.0	36.0	36.0	36.0	36.0
26	34.88987566039133	36.0	36.0	36.0	32.0	36.0
27	34.87084989930146	36.0	36.0	36.0	32.0	36.0
28	34.85733124653177	36.0	36.0	36.0	32.0	36.0
29	34.836413102602805	36.0	36.0	36.0	32.0	36.0
30	34.82829505448143	36.0	36.0	36.0	32.0	36.0
31	34.81053362453479	36.0	36.0	36.0	32.0	36.0
32	34.78196184334544	36.0	36.0	36.0	32.0	36.0
33	34.76921522792243	36.0	36.0	36.0	32.0	36.0
34	34.76218232880106	36.0	36.0	36.0	32.0	36.0
35	34.726920471468446	36.0	36.0	36.0	32.0	36.0
36	34.71162233889377	36.0	36.0	36.0	32.0	36.0
37	34.69198086773524	36.0	36.0	36.0	32.0	36.0
38	34.67099507340526	36.0	36.0	36.0	32.0	36.0
39	34.66071906891108	36.0	36.0	36.0	32.0	36.0
40	34.648692838839374	36.0	36.0	36.0	32.0	36.0
41	34.63008440758818	36.0	36.0	36.0	32.0	36.0
42	34.59453137927283	36.0	36.0	36.0	32.0	36.0
43	34.579629092963536	36.0	36.0	36.0	32.0	36.0
44	34.541842230122896	36.0	36.0	36.0	32.0	36.0
45	34.51571134853761	36.0	36.0	36.0	32.0	36.0
46	34.49515019760317	36.0	36.0	36.0	32.0	36.0
47	34.472539879454295	36.0	36.0	36.0	32.0	36.0
48	34.435247138799426	36.0	36.0	36.0	32.0	36.0
49	34.41866867667588	36.0	36.0	36.0	32.0	36.0
50	33.84988513144747	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	1.0
16	0.0
17	0.0
18	3.0
19	7.0
20	25.0
21	80.0
22	249.0
23	671.0
24	1751.0
25	4019.0
26	7937.0
27	14344.0
28	23406.0
29	35847.0
30	50842.0
31	71362.0
32	102370.0
33	161629.0
34	340133.0
35	1373042.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.67453437841999	18.093710371537266	12.430594476083723	26.80116077395902
2	15.56900542667408	20.426957478863763	37.78852060965977	26.215516484802386
3	18.194459046217275	23.89973991068286	29.20720753664791	28.698593506451953
4	12.828892937755231	16.830322738122554	35.60870276699282	34.73208155712939
5	14.411683772771443	36.628669691431895	33.85495754023142	15.10468899556524
6	33.89416253411668	35.48404201099504	17.108551258780288	13.51324419610799
7	29.871838940703455	30.85651885345264	20.691351162582347	18.580291043261557
8	27.39896092640825	34.09401943029221	19.42695539370248	19.08006424959707
9	27.436132667245356	14.838970077693503	18.321396133563166	39.403501121497975
10	15.656120097234805	27.634257807536155	32.41312541882	24.296496676409042
11	36.498081331434854	21.394102979592862	23.003133406316636	19.104682282655645
12	24.84231264127183	23.50648964787461	29.366028024674147	22.285169686179415
13	29.622143357340192	20.266918619852376	24.976642220991103	25.134295801816332
14	23.35200122136623	19.86265115092741	25.65975260020277	31.125595027503593
15	24.948505164111506	27.519041432658103	22.43990897779208	25.092544425438312
16	25.202861452667186	26.123462997668785	24.231841660191066	24.441833889472964
17	23.842469641882545	26.295939421808477	25.51082909223218	24.350761844076796
18	24.069744321103624	26.063888869843055	26.34491396234867	23.52145284670465
19	25.487105742147754	25.281914762323115	25.4201866968229	23.81079279870623
20	25.65849738630567	25.27969911889521	25.215750913741687	23.846052581057435
21	25.982324961443844	24.683711520406195	25.019403780560385	24.31455973758958
22	25.43456280431281	25.164006662656398	25.22000113360235	24.181429399428445
23	24.75802245551613	25.127268189512016	25.22559049377361	24.889118861198252
24	24.539816447670947	25.325705279574933	25.624693172873602	24.509785099880517
25	24.933125502815766	25.01371315731734	25.415234403569077	24.637926936297813
26	24.595710834451374	25.59223442989022	25.5986798347965	24.2133749008619
27	24.7816769550335	25.504026359673503	25.225408487171237	24.488888198121764
28	24.82810070131074	25.416914653289556	25.194115243154886	24.560869402244816
29	24.60642950654214	25.250861192578018	25.241170490532365	24.901538810347475
30	24.784082192757666	25.471993081122196	25.25824656547381	24.485678160646323
31	24.90610526084818	25.14541342027358	25.190029420961107	24.758451897917126
32	24.79175113718072	25.50845726024354	25.114746574744462	24.585045027831274
33	24.63532136197889	25.462936509278844	25.25279860084814	24.648943527894126
34	24.87219243905828	25.296572051283928	25.437864315543884	24.393371194113904
35	24.949854912938623	25.10742110719621	25.630264777030348	24.31245920283482
36	24.522955986055532	25.37034852944	25.361434382277032	24.74526110222744
37	24.740531484770152	25.192432958729594	25.25556020286655	24.811475353633703
38	24.78572973032277	25.436734671487297	25.18631650789631	24.591219090293624
39	24.946951627014855	25.185200761007593	25.25587097611749	24.61197663586006
40	25.226692923139932	25.09056517698664	25.508044930384234	24.174696969489194
41	24.682672317799423	24.901857889126383	25.790027993585795	24.6254417994884
42	24.817363098889505	25.213811564245862	25.671740853527535	24.2970844833371
43	24.65456556717619	24.893128793973524	25.734979521685077	24.717326117165218
44	24.251470729399504	25.115441401661116	25.668033405098527	24.965054463840854
45	24.41507910859359	25.023483685295307	25.805724671102702	24.755712535008403
46	24.43177662709993	24.91511617701911	25.521553268827113	25.13155392705385
47	24.37407464799484	24.934119736835488	25.897651440040846	24.794154175128824
48	24.301520970598105	25.38394519861662	25.467686802743728	24.84684702804155
49	24.275212105449125	25.200152305448302	25.653274727123215	24.87136086197936
50	24.158001097966295	25.491696458487322	25.43350698753612	24.91679545601027
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	19.0
1	24.5
2	30.0
3	93.5
4	157.0
5	198.5
6	240.0
7	243.0
8	246.0
9	312.5
10	379.0
11	594.5
12	810.0
13	1405.0
14	2000.0
15	2667.0
16	3334.0
17	3305.0
18	3276.0
19	3422.5
20	3569.0
21	4165.5
22	4762.0
23	6246.0
24	7730.0
25	10355.0
26	12980.0
27	16916.5
28	20853.0
29	25886.5
30	30920.0
31	35474.5
32	40029.0
33	48659.0
34	57289.0
35	66194.0
36	75099.0
37	82007.0
38	88915.0
39	97266.5
40	105618.0
41	113574.0
42	121530.0
43	127306.5
44	133083.0
45	140807.5
46	148532.0
47	153536.5
48	158541.0
49	163965.5
50	169390.0
51	166130.5
52	162871.0
53	163533.0
54	164195.0
55	157784.0
56	151373.0
57	144313.0
58	137253.0
59	125708.0
60	114163.0
61	98388.5
62	82614.0
63	72289.0
64	61964.0
65	52056.5
66	42149.0
67	35144.5
68	28140.0
69	23644.5
70	19149.0
71	15335.0
72	11521.0
73	9873.0
74	8225.0
75	6725.5
76	5226.0
77	4241.5
78	3257.0
79	2982.0
80	2707.0
81	2381.0
82	2055.0
83	1428.0
84	801.0
85	593.0
86	385.0
87	269.5
88	154.0
89	110.0
90	66.0
91	52.5
92	39.0
93	31.5
94	24.0
95	28.5
96	33.0
97	24.5
98	16.0
99	11.5
100	7.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009690462847588217
2	2.742583824789118E-4
3	3.6567784330521577E-4
4	0.0
5	0.0
6	2.2854865206575983E-4
7	9.141946082630394E-5
8	0.0
9	8.684848778498875E-4
10	0.0010970335299156472
11	1.371291912394559E-4
12	1.371291912394559E-4
13	4.5709730413151967E-4
14	1.8283892165260788E-4
15	0.005485167649578237
16	8.227751474367355E-4
17	0.0
18	5.942264953709756E-4
19	0.0
20	4.5709730413151967E-4
21	0.0
22	9.141946082630394E-5
23	6.856459561972796E-4
24	1.8283892165260788E-4
25	0.001736969755699775
26	0.005165199536686173
27	0.006125103875362364
28	0.004570973041315197
29	0.002468325442310206
30	0.0036567784330521574
31	0.0074049763269306195
32	0.00324539085933379
33	0.004890941154207261
34	0.002468325442310206
35	0.00301684220726803
36	0.008410590396019962
37	0.0033368103201600933
38	0.008090622283127898
39	0.004205295198009981
40	0.003062551937681182
41	0.0033825200505732456
42	0.0020112281381786867
43	0.001736969755699775
44	0.001279872451568255
45	0.001325582181981407
46	0.0010056140690893433
47	0.0016912600252866228
48	0.001645550294873471
49	4.1138757371836777E-4
50	0.0016912600252866228
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2187718.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.52751603330554
#Duplication Level	Percentage of deduplicated	Percentage of total
1	58.02883919750235	20.03591675785871
2	14.865735757739598	10.265538594444749
3	7.72936960858631	8.006277992634251
4	4.844531639829179	6.690785754722318
5	3.2719541197741924	5.648622416537178
6	2.30687502803698	4.77903987104274
7	1.7004514571875038	4.109865546632952
8	1.3014199868864051	3.5947839570627713
9	0.9686045485455501	3.0099158171855245
>10	4.799297826922045	27.217266800264444
>50	0.12534772279356926	2.923075762736297
>100	0.0561078966123516	3.404608561670159
>500	0.0013320087126179054	0.2676213507436674
>1k	1.332008712617885E-4	0.04668081646413795
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	4.570973041315197E-5
7	0.0	0.0	0.0	0.0	4.570973041315197E-5
8	0.0	0.0	0.0	0.0	4.570973041315197E-5
9	0.0	0.0	0.0	4.570973041315197E-5	4.570973041315197E-5
10	0.0	0.0	0.0	4.570973041315197E-5	4.570973041315197E-5
11	0.0	0.0	0.0	4.570973041315197E-5	4.570973041315197E-5
12	0.0	0.0	0.0	4.570973041315197E-5	4.570973041315197E-5
13	0.0	0.0	0.0	4.570973041315197E-5	1.371291912394559E-4
14	0.0	0.0	0.0	4.570973041315197E-5	1.371291912394559E-4
15	0.0	0.0	0.0	4.570973041315197E-5	1.371291912394559E-4
16	0.0	0.0	0.0	1.371291912394559E-4	1.371291912394559E-4
17	0.0	0.0	0.0	1.371291912394559E-4	1.371291912394559E-4
18	0.0	0.0	0.0	1.8283892165260788E-4	1.8283892165260788E-4
19	0.0	0.0	0.0	2.2854865206575983E-4	1.8283892165260788E-4
20	0.0	0.0	0.0	3.6567784330521577E-4	1.8283892165260788E-4
21	0.0	0.0	0.0	4.113875737183677E-4	1.8283892165260788E-4
22	0.0	0.0	0.0	5.485167649578236E-4	1.8283892165260788E-4
23	0.0	0.0	0.0	7.313556866104315E-4	1.8283892165260788E-4
24	0.0	0.0	0.0	0.0010056140690893433	1.8283892165260788E-4
25	0.0	0.0	0.0	0.0011884529907419512	1.8283892165260788E-4
26	0.0	0.0	0.0	0.001599840564460319	2.2854865206575983E-4
27	0.0	0.0	0.0	0.00255974490313651	2.742583824789118E-4
28	0.0	0.0	0.0	0.005576587110404541	2.742583824789118E-4
29	0.0	0.0	0.0	0.010604657455851256	2.742583824789118E-4
30	0.0	0.0	0.0	0.021255024642115667	2.742583824789118E-4
31	0.0	0.0	0.0	0.055080225147848126	2.742583824789118E-4
32	0.0	0.0	0.0	0.09407062519026675	2.742583824789118E-4
33	0.0	0.0	0.0	0.13315244469351167	2.742583824789118E-4
34	0.0	0.0	0.0	0.1764395593947666	3.1996811289206376E-4
35	0.0	0.0	0.0	0.22800013530080201	3.1996811289206376E-4
36	0.0	0.0	0.0	0.3011357039618452	3.1996811289206376E-4
37	0.0	0.0	0.0	0.4197524543839745	3.1996811289206376E-4
38	0.0	0.0	0.0	0.579919349751659	3.1996811289206376E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	2190	0.0	29.832159	1
GGTATCA	780	0.0	23.407545	1
TATCAAC	3335	0.0	19.39117	2
ATCAACG	3350	0.0	19.173021	3
TCAACGC	3380	0.0	19.003283	4
CAACGCA	3460	0.0	18.5639	5
TAGACGC	85	1.433025E-4	18.115133	4
AACGCAG	3585	0.0	18.039337	6
CGCGGAT	90	2.2179789E-4	17.10952	42
ACGATAT	105	3.7773883E-5	16.761496	15
CGTGATT	330	0.0	16.667784	28
TAGGACG	665	0.0	16.53906	4
TCCCGAT	80	0.0019911928	16.497335	17
GTATAAT	230	1.8189894E-12	16.258987	1
GTATAAG	285	0.0	16.208647	1
GTAGGAC	950	0.0	15.744824	3
TAGGACC	875	0.0	15.58641	4
ACGCAGA	4160	0.0	15.493044	7
GTCTTAT	245	5.456968E-12	15.263538	1
GTATTAC	235	5.0931703E-11	14.976988	1
>>END_MODULE