FastQCFastQC Report
Thu 2 Feb 2017
SRR4063874_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063874_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6400629
Sequences flagged as poor quality0
Sequence length25
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT77690.1213786957500583No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20350.014.6553161
GTATCAA67250.013.4171811
TCGCGTA2850.011.0005529
AATAGCG3150.010.2520785
ACGAACG4200.010.18051515
CGCGTAA3300.010.07610610
GCGTAAC3500.010.04336211
ATAGCGT3500.010.0416356
GCGTTAT3050.09.9650641
CGAACGA4250.09.83701916
CGAACCT6700.09.78529816
TAGGACA5800.09.6616344
CGTCGTA5050.09.59439110
GTATTAG6350.09.5727391
ATACCGT6900.09.3611786
TATACTG7350.09.3044065
CGATAAC4700.09.29819410
TATACCG2357.2759576E-129.2961595
TAATTGC8100.09.26640413
ACCGTCG5650.09.2481088
Produced by FastQC (version 0.11.5)