##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063874_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6400629
Sequences flagged as poor quality	0
Sequence length	25
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.26129635071803	32.0	32.0	32.0	32.0	32.0
2	30.783214118487418	32.0	32.0	32.0	32.0	32.0
3	30.8095876202167	32.0	32.0	32.0	32.0	32.0
4	30.84980819853799	32.0	32.0	32.0	32.0	32.0
5	30.701274046660103	32.0	32.0	32.0	32.0	32.0
6	34.391919450416516	36.0	36.0	36.0	32.0	36.0
7	34.344280538678305	36.0	36.0	36.0	32.0	36.0
8	34.29837036328773	36.0	36.0	36.0	32.0	36.0
9	34.481841237790846	36.0	36.0	36.0	32.0	36.0
10	34.112798289043155	36.0	36.0	36.0	32.0	36.0
11	34.45024543681566	36.0	36.0	36.0	32.0	36.0
12	34.24537838390571	36.0	36.0	36.0	32.0	36.0
13	34.33543812647163	36.0	36.0	36.0	32.0	36.0
14	34.22224550118434	36.0	36.0	36.0	32.0	36.0
15	34.14223570839678	36.0	36.0	36.0	32.0	36.0
16	34.14864914057665	36.0	36.0	36.0	32.0	36.0
17	34.077501914264985	36.0	36.0	36.0	32.0	36.0
18	34.068684343366876	36.0	36.0	36.0	32.0	36.0
19	34.0933411388162	36.0	36.0	36.0	32.0	36.0
20	34.074747966176446	36.0	36.0	36.0	32.0	36.0
21	34.06891697675338	36.0	36.0	36.0	32.0	36.0
22	34.04146701831961	36.0	36.0	36.0	32.0	36.0
23	33.97624249116767	36.0	36.0	36.0	32.0	36.0
24	33.96287646104781	36.0	36.0	36.0	32.0	36.0
25	33.537447647723376	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	19.0
4	61.0
5	78.0
6	257.0
7	124.0
8	378.0
9	454.0
10	558.0
11	184.0
12	373.0
13	307.0
14	470.0
15	904.0
16	1613.0
17	2159.0
18	2895.0
19	4156.0
20	6536.0
21	9694.0
22	15477.0
23	24046.0
24	36292.0
25	53172.0
26	74692.0
27	98462.0
28	133656.0
29	178756.0
30	236115.0
31	318468.0
32	448540.0
33	631568.0
34	1331609.0
35	2788556.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.50349190312667	16.678594711200397	11.65187344279626	26.166039942876672
2	17.114400312111783	18.791126188250466	37.149489451306046	26.944984048331705
3	19.140327477182666	22.73116801528379	28.001063040787237	30.12744146674631
4	13.277149169268073	15.306341185992082	34.390333073973736	37.02617657076611
5	15.466636750098164	35.62760184693144	33.11932882720229	15.786432575768112
6	35.42096080991233	34.03856896528825	16.55985705591117	13.980613168888253
7	31.474035099727605	29.72497144511845	19.9643667582892	18.83662669686475
8	28.758927798340615	32.64400680558538	18.466183223788164	20.130882172285837
9	27.385995308134987	14.681445249486671	17.84751626271443	40.08504317966391
10	16.234587750613926	26.59358590301208	30.876601800766963	26.29522454560703
11	37.16091496917025	20.870733279683755	22.199951602828047	19.768400148317948
12	25.40706035597721	22.791299599329825	28.191099772329792	23.61054027236317
13	29.739795665517544	19.453892793731793	24.81195496330556	25.9943565774451
14	24.181525994162527	18.858589773434893	24.69793690472077	32.2619473276818
15	25.670959823623456	26.636853699109658	21.661279311477404	26.03090716578948
16	26.285330495465303	25.500523657941848	22.866943981029184	25.34720186556366
17	24.75532996257059	25.63809939508243	24.438613545313476	25.167957097033504
18	25.203494830913108	25.05526831891278	25.025388480892463	24.71584836928165
19	26.042826283281258	24.60236355403678	24.6572433875273	24.697566775154662
20	26.431684447683534	24.14214952378168	24.25486956357221	25.171296464962577
21	26.775817190488283	23.975674096940875	24.026879447438763	25.22162926513208
22	26.47253213750466	23.774631326552072	24.280528772672938	25.47230776327033
23	25.31239357415401	23.905453662988027	24.67337722182766	26.108775541030298
24	25.291205247378066	24.416944528052596	24.483271637030292	25.80857858753904
25	25.465359235329977	24.1374031884392	24.556226109338958	25.841011466891867
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	166.0
1	166.0
2	498.0
3	830.0
4	830.0
5	830.0
6	2082.0
7	3334.0
8	3334.0
9	3334.0
10	4500.0
11	5666.0
12	5666.0
13	5666.0
14	8293.0
15	10920.0
16	10920.0
17	10920.0
18	19963.5
19	29007.0
20	29007.0
21	29007.0
22	46740.5
23	64474.0
24	64474.0
25	64474.0
26	99338.0
27	134202.0
28	134202.0
29	134202.0
30	176968.0
31	219734.0
32	219734.0
33	219734.0
34	283674.0
35	347614.0
36	347614.0
37	347614.0
38	420982.5
39	494351.0
40	494351.0
41	494351.0
42	579961.0
43	665571.0
44	665571.0
45	665571.0
46	748557.0
47	831543.0
48	831543.0
49	831543.0
50	867046.5
51	902550.0
52	902550.0
53	902550.0
54	869181.5
55	835813.0
56	835813.0
57	835813.0
58	768953.5
59	702094.0
60	702094.0
61	702094.0
62	608436.0
63	514778.0
64	514778.0
65	514778.0
66	418439.0
67	322100.0
68	322100.0
69	322100.0
70	244459.0
71	166818.0
72	166818.0
73	166818.0
74	124151.0
75	81484.0
76	81484.0
77	81484.0
78	60727.0
79	39970.0
80	39970.0
81	39970.0
82	27219.0
83	14468.0
84	14468.0
85	14468.0
86	10364.0
87	6260.0
88	6260.0
89	6260.0
90	4507.5
91	2755.0
92	2755.0
93	2755.0
94	1995.0
95	1235.0
96	1235.0
97	1235.0
98	2063.5
99	2892.0
100	2892.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.039714846775215376
2	0.0055932002932836756
3	0.001906062669778236
4	0.0038902426620883667
5	0.00949906641987842
6	0.01443608120389418
7	0.024185123055874665
8	0.036793258912522504
9	0.04957325287874051
10	0.057619337099525686
11	0.05563515710721555
12	0.06399371061812831
13	0.06493111848851106
14	0.06821204603485065
15	0.06030657299462287
16	0.06683718115828928
17	0.06335314857336677
18	0.07830480410597146
19	0.07557069781735513
20	0.08027336063377521
21	0.07519573466920204
22	0.07911722426030317
23	0.08328868928350636
24	0.07896098961523937
25	0.0794609404794435
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	6400629.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.69168790351525
#Duplication Level	Percentage of deduplicated	Percentage of total
1	65.05766441061107	23.87075518285773
2	15.442554900349368	11.332268096730381
3	6.789346195659765	7.473377150402002
4	3.4810927608095543	5.109086765712418
5	2.125313401316884	3.899066800913879
6	1.397622155477839	3.0768669561498685
7	1.0039478985188968	2.578558007469176
8	0.6936353756117427	2.036052217662691
9	0.5391012161269987	1.7802480213483625
>10	3.127603854019692	21.15613533978598
>50	0.2216131833683176	5.5476205215574605
>100	0.1075080201704972	7.121166827389276
>500	0.00859320208092284	2.1737226310282667
>1k	0.0043606741708161955	2.723208436798564
>5k	4.2751707539587416E-5	0.12186704419380301
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	7769	0.1213786957500583	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.124692901275796E-5
2	0.0	0.0	0.0	0.0	3.124692901275796E-5
3	0.0	0.0	0.0	0.0	3.124692901275796E-5
4	0.0	0.0	0.0	0.0	4.6870393519136944E-5
5	1.562346450637898E-5	0.0	0.0	0.0	4.6870393519136944E-5
6	3.124692901275796E-5	0.0	0.0	0.0	7.811732253189491E-5
7	3.124692901275796E-5	0.0	0.0	0.0	9.374078703827389E-5
8	3.124692901275796E-5	0.0	0.0	1.562346450637898E-5	9.374078703827389E-5
9	3.124692901275796E-5	0.0	0.0	3.124692901275796E-5	1.0936425154465288E-4
10	3.124692901275796E-5	0.0	0.0	9.374078703827389E-5	1.2498771605103185E-4
11	3.124692901275796E-5	0.0	0.0	9.374078703827389E-5	1.2498771605103185E-4
12	3.124692901275796E-5	0.0	0.0	1.0936425154465288E-4	5.311977932168854E-4
13	3.124692901275796E-5	0.0	0.0	1.0936425154465288E-4	6.874324382806752E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2035	0.0	14.655316	1
GTATCAA	6725	0.0	13.417181	1
TCGCGTA	285	0.0	11.000552	9
AATAGCG	315	0.0	10.252078	5
ACGAACG	420	0.0	10.180515	15
CGCGTAA	330	0.0	10.076106	10
GCGTAAC	350	0.0	10.043362	11
ATAGCGT	350	0.0	10.041635	6
GCGTTAT	305	0.0	9.965064	1
CGAACGA	425	0.0	9.837019	16
CGAACCT	670	0.0	9.785298	16
TAGGACA	580	0.0	9.661634	4
CGTCGTA	505	0.0	9.594391	10
GTATTAG	635	0.0	9.572739	1
ATACCGT	690	0.0	9.361178	6
TATACTG	735	0.0	9.304406	5
CGATAAC	470	0.0	9.298194	10
TATACCG	235	7.2759576E-12	9.296159	5
TAATTGC	810	0.0	9.266404	13
ACCGTCG	565	0.0	9.248108	8
>>END_MODULE