FastQCFastQC Report
Thu 2 Feb 2017
SRR4063874_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063874_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6400629
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA68550.028.6281221
GGTATCA24900.026.9485861
TCAACGC103150.018.7671384
TATCAAC104850.018.63072
CAACGCA105650.018.3022295
ATCAACG107400.018.0449713
AACGCAG110800.017.4713926
GTATAGG8000.016.5004861
ACGCAGA134700.014.4040817
AGAGTAC137400.014.08956111
TCTATAC6000.013.9321123
CGCAGAG142200.013.6752058
TCTAGCG9900.013.55616828
CGTCGTA9900.013.55489610
CAGAGTA143500.013.53662410
CGCAATA9850.013.40266736
ATACGAA9650.013.22277440
TTAACGG3051.8189894E-1112.98530935
CTAGCGG10200.012.94186129
ATACCGT12400.012.595676
Produced by FastQC (version 0.11.5)