FastQCFastQC Report
Thu 2 Feb 2017
SRR4063873_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063873_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3055577
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT98810.3233759123072336No Hit
TATCAACGCAGAGTACTTTTTTTTT65190.2133475935968886No Hit
GGTATCAACGCAGAGTACTTTTTTT36510.11948643414975307No Hit
ACGCAGAGTACTTTTTTTTTTTTTT30740.10060293031397997No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13050.015.5038371
GTATCAA47600.013.6296221
CGAACGA2000.013.30097416
CGTCGTA3900.012.66655710
GCGTAAC2500.012.54030211
CGCGTAA2500.012.15989610
TCGCGTA2550.011.9222469
ACGAACG2250.011.82289415
CCGTCGT4200.011.3101679
CGCGGTA1201.2754572E-711.08414618
TAATCGG600.00587537111.08396315
GTCCTAA4900.011.0496581
GTCGTAG4300.011.04677911
CGGAATT1553.1286618E-1011.03288615
AACCGCG1303.2658136E-810.9625217
GATAACG2600.010.961802511
CGACCCG2450.010.8566935
CGAGCCG3950.010.823400515
TCCAACG2650.010.75550518
AGAACCG2350.010.5102035
Produced by FastQC (version 0.11.5)