##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063873_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3055577
Sequences flagged as poor quality	0
Sequence length	25
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.281560896681707	32.0	32.0	32.0	32.0	32.0
2	30.94638230357147	32.0	32.0	32.0	32.0	32.0
3	30.956290415852717	32.0	32.0	32.0	32.0	32.0
4	30.930824194579287	32.0	32.0	32.0	32.0	32.0
5	30.910568773099158	32.0	32.0	32.0	32.0	32.0
6	34.38303272998848	36.0	36.0	36.0	32.0	36.0
7	34.378413307862964	36.0	36.0	36.0	32.0	36.0
8	34.33215101435834	36.0	36.0	36.0	32.0	36.0
9	34.490534520975906	36.0	36.0	36.0	32.0	36.0
10	34.183410858243796	36.0	36.0	36.0	32.0	36.0
11	34.46946746882831	36.0	36.0	36.0	32.0	36.0
12	34.282915141722825	36.0	36.0	36.0	32.0	36.0
13	34.386642195565685	36.0	36.0	36.0	32.0	36.0
14	34.272253980181155	36.0	36.0	36.0	32.0	36.0
15	34.19713068922825	36.0	36.0	36.0	32.0	36.0
16	34.204396420054216	36.0	36.0	36.0	32.0	36.0
17	34.14434295061129	36.0	36.0	36.0	32.0	36.0
18	34.13625380738237	36.0	36.0	36.0	32.0	36.0
19	34.13084566351952	36.0	36.0	36.0	32.0	36.0
20	34.095937690328206	36.0	36.0	36.0	32.0	36.0
21	34.082889418266994	36.0	36.0	36.0	32.0	36.0
22	34.06414500436415	36.0	36.0	36.0	32.0	36.0
23	34.025841600457134	36.0	36.0	36.0	32.0	36.0
24	33.99851779222058	36.0	36.0	36.0	32.0	36.0
25	33.362863380631545	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	3.0
4	29.0
5	39.0
6	135.0
7	42.0
8	146.0
9	181.0
10	282.0
11	84.0
12	173.0
13	167.0
14	394.0
15	962.0
16	1573.0
17	2040.0
18	2601.0
19	3156.0
20	4073.0
21	5125.0
22	6887.0
23	9361.0
24	12693.0
25	17942.0
26	25263.0
27	34987.0
28	49435.0
29	69677.0
30	99596.0
31	150942.0
32	242278.0
33	387528.0
34	844752.0
35	1083030.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.331394079240084	18.512204696181524	12.519320241785808	25.637080982792586
2	15.764450137754391	20.3746893244111	38.60471068305101	25.256149854783505
3	18.02958228401153	24.694252580145275	29.581498554267732	27.69466658157546
4	12.24322348254512	16.613243492507554	37.18148191543189	33.962051109515436
5	13.876086058585141	37.17418242445785	34.30835586784206	14.641375649114952
6	32.30877645933647	36.954811899815624	17.638084751704223	13.098326889143683
7	29.056492725557476	31.401741833396507	21.447346627202744	18.094418813843273
8	27.28725694165945	33.6300913550702	19.778921057499186	19.303730645771168
9	26.86648237285216	15.156829277636133	19.304553331973448	38.67213501753825
10	15.185003148411502	27.63408090779035	32.45634055366855	24.72457539012961
11	35.56152236626249	22.04261394334513	23.636592849213965	18.759270841178417
12	24.276448674520775	24.343547867365626	29.659480601816558	21.72052285629704
13	28.479748548695934	20.764152077214256	25.89993623988213	24.85616313420768
14	22.98078125951751	20.555203691926398	26.385241987934172	30.078773060621916
15	24.224706502991864	28.233870230949915	23.271723330339228	24.26969993571899
16	24.631754202172583	26.791762769112776	24.748174203620056	23.828308825094584
17	22.828550825916643	26.718228194143624	26.29716494558401	24.15605603435572
18	23.371273307996347	26.162534013003874	27.261627305253032	23.204565373746743
19	24.609145740756265	25.692068154187975	26.389900540789807	23.308885564265953
20	24.85359622690947	25.57261234115027	25.938195991091312	23.635595440848945
21	25.799684940344992	25.150668601128583	25.723310811917244	23.326335646609177
22	24.8328401373634	25.386813571923366	26.04251583986061	23.737830450852627
23	23.75456627099142	25.30217716918548	26.441415312275122	24.50184124754798
24	23.871112271242882	25.782818746841524	26.33510770175021	24.010961280165382
25	23.9636357919601	25.436375081798836	26.495059972710795	24.10492915353027
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	170.0
1	170.0
2	550.5
3	931.0
4	931.0
5	931.0
6	2488.5
7	4046.0
8	4046.0
9	4046.0
10	4761.0
11	5476.0
12	5476.0
13	5476.0
14	7929.0
15	10382.0
16	10382.0
17	10382.0
18	19098.5
19	27815.0
20	27815.0
21	27815.0
22	44095.5
23	60376.0
24	60376.0
25	60376.0
26	89180.5
27	117985.0
28	117985.0
29	117985.0
30	147706.5
31	177428.0
32	177428.0
33	177428.0
34	207461.5
35	237495.0
36	237495.0
37	237495.0
38	261035.5
39	284576.0
40	284576.0
41	284576.0
42	311821.5
43	339067.0
44	339067.0
45	339067.0
46	366206.0
47	393345.0
48	393345.0
49	393345.0
50	391243.5
51	389142.0
52	389142.0
53	389142.0
54	357817.5
55	326493.0
56	326493.0
57	326493.0
58	294106.0
59	261719.0
60	261719.0
61	261719.0
62	224106.5
63	186494.0
64	186494.0
65	186494.0
66	151090.0
67	115686.0
68	115686.0
69	115686.0
70	86728.0
71	57770.0
72	57770.0
73	57770.0
74	43520.5
75	29271.0
76	29271.0
77	29271.0
78	23377.5
79	17484.0
80	17484.0
81	17484.0
82	11983.0
83	6482.0
84	6482.0
85	6482.0
86	4846.5
87	3211.0
88	3211.0
89	3211.0
90	2180.5
91	1150.0
92	1150.0
93	1150.0
94	756.0
95	362.0
96	362.0
97	362.0
98	791.5
99	1221.0
100	1221.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.037243374982859206
2	0.004549058982967865
3	0.001047265377373897
4	0.0032399772612504937
5	0.008607212320291716
6	0.012109005925885683
7	0.020781672332263267
8	0.033119767559449495
9	0.04568695208793625
10	0.05419598327909917
11	0.05390143989171276
12	0.061821384308102856
13	0.06362137167546425
14	0.06529045087065388
15	0.05884322339119583
16	0.064734091138924
17	0.06149411387767351
18	0.07635219141916567
19	0.07412675249224615
20	0.07779218131305478
21	0.07157404313489726
22	0.07730127566741077
23	0.08129397491864876
24	0.0747485663100619
25	0.07707218636611023
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	3055577.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.92813354605009
#Duplication Level	Percentage of deduplicated	Percentage of total
1	61.6361305238343	22.761072592459275
2	16.63685683396907	12.287361419026517
3	7.773172909959168	8.61146301886533
4	4.296833213198289	6.346961228883598
5	2.5620792754126835	4.7306402819003415
6	1.6678958230889254	3.6955367817556177
7	1.1428319572935388	2.954185579774073
8	0.8401325606739399	2.4819621917561827
9	0.5873782767986588	1.9521705102902687
>10	2.600917121990407	16.120032081773967
>50	0.13540176408065363	3.4851607663075628
>100	0.1020791408127886	7.804813880602672
>500	0.012608082728541352	3.1548841462295605
>1k	0.005504937529236494	3.0760284366374435
>5k	1.7757862997537074E-4	0.5377270837377186
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	9881	0.3233759123072336	No Hit
TATCAACGCAGAGTACTTTTTTTTT	6519	0.2133475935968886	No Hit
GGTATCAACGCAGAGTACTTTTTTT	3651	0.11948643414975307	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	3074	0.10060293031397997	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.272704304293428E-5
2	0.0	0.0	0.0	0.0	3.272704304293428E-5
3	0.0	0.0	0.0	0.0	3.272704304293428E-5
4	0.0	0.0	0.0	0.0	6.545408608586856E-5
5	3.272704304293428E-5	0.0	0.0	0.0	6.545408608586856E-5
6	3.272704304293428E-5	0.0	0.0	0.0	9.818112912880284E-5
7	3.272704304293428E-5	0.0	0.0	0.0	9.818112912880284E-5
8	3.272704304293428E-5	0.0	0.0	0.0	1.3090817217173713E-4
9	3.272704304293428E-5	0.0	0.0	0.0	1.3090817217173713E-4
10	3.272704304293428E-5	0.0	0.0	0.0	1.3090817217173713E-4
11	3.272704304293428E-5	0.0	0.0	0.0	1.3090817217173713E-4
12	3.272704304293428E-5	0.0	0.0	0.0	1.3090817217173713E-4
13	3.272704304293428E-5	0.0	0.0	0.0	1.3090817217173713E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1305	0.0	15.503837	1
GTATCAA	4760	0.0	13.629622	1
CGAACGA	200	0.0	13.300974	16
CGTCGTA	390	0.0	12.666557	10
GCGTAAC	250	0.0	12.540302	11
CGCGTAA	250	0.0	12.159896	10
TCGCGTA	255	0.0	11.922246	9
ACGAACG	225	0.0	11.822894	15
CCGTCGT	420	0.0	11.310167	9
CGCGGTA	120	1.2754572E-7	11.084146	18
TAATCGG	60	0.005875371	11.083963	15
GTCCTAA	490	0.0	11.049658	1
GTCGTAG	430	0.0	11.046779	11
CGGAATT	155	3.1286618E-10	11.032886	15
AACCGCG	130	3.2658136E-8	10.962521	7
GATAACG	260	0.0	10.9618025	11
CGACCCG	245	0.0	10.856693	5
CGAGCCG	395	0.0	10.8234005	15
TCCAACG	265	0.0	10.755505	18
AGAACCG	235	0.0	10.510203	5
>>END_MODULE