FastQCFastQC Report
Thu 2 Feb 2017
SRR4063873_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063873_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3055577
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA46850.027.0010071
GGTATCA18550.022.652241
TACGCGG550.0044843620.0006135
CGTCTTA4650.018.9243815
TCAACGC68350.018.3779894
AACGCAG68650.018.1694986
TATCAAC70600.017.9795422
GTAAACG5400.017.9270627
CAACGCA69800.017.9016595
ATCAACG70150.017.8750613
CGCGGGA5600.017.28539544
AATACCG1154.2792926E-617.2165095
TAAACGC5700.016.9835328
CGCAATA3900.016.92359436
ATACCGT8350.016.861416
TAGGACG7750.016.1798134
ACCGTCG8250.015.999448
CGTCGTA8150.015.92634410
TACCGTC8300.015.9030587
CCGTCGT8500.015.7881999
Produced by FastQC (version 0.11.5)