##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063873_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3055577
Sequences flagged as poor quality	0
Sequence length	50
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.198894349577838	32.0	32.0	32.0	32.0	32.0
2	31.062294617350503	32.0	32.0	32.0	32.0	32.0
3	31.317003302485915	32.0	32.0	32.0	32.0	32.0
4	31.46564789563477	32.0	32.0	32.0	32.0	32.0
5	31.353106467289155	32.0	32.0	32.0	32.0	32.0
6	34.99731474611833	36.0	36.0	36.0	36.0	36.0
7	35.02918401336311	36.0	36.0	36.0	36.0	36.0
8	34.95565289305424	36.0	36.0	36.0	36.0	36.0
9	35.131823547565645	36.0	36.0	36.0	36.0	36.0
10	34.91327006323192	36.0	36.0	36.0	32.0	36.0
11	35.139672474298635	36.0	36.0	36.0	36.0	36.0
12	35.022330970549916	36.0	36.0	36.0	36.0	36.0
13	35.08732458714017	36.0	36.0	36.0	36.0	36.0
14	35.023520598564524	36.0	36.0	36.0	36.0	36.0
15	34.968092769385294	36.0	36.0	36.0	36.0	36.0
16	34.99685264027056	36.0	36.0	36.0	36.0	36.0
17	34.95859570876466	36.0	36.0	36.0	36.0	36.0
18	34.96571645878994	36.0	36.0	36.0	36.0	36.0
19	34.96774717181076	36.0	36.0	36.0	36.0	36.0
20	34.95712233728687	36.0	36.0	36.0	36.0	36.0
21	34.96975432136058	36.0	36.0	36.0	36.0	36.0
22	34.948930758413226	36.0	36.0	36.0	36.0	36.0
23	34.89556506021612	36.0	36.0	36.0	32.0	36.0
24	34.87920513866939	36.0	36.0	36.0	32.0	36.0
25	34.850586321339634	36.0	36.0	36.0	32.0	36.0
26	34.78983282044602	36.0	36.0	36.0	32.0	36.0
27	34.77848766370476	36.0	36.0	36.0	32.0	36.0
28	34.754466014111244	36.0	36.0	36.0	32.0	36.0
29	34.74079003736446	36.0	36.0	36.0	32.0	36.0
30	34.72228485814627	36.0	36.0	36.0	32.0	36.0
31	34.71777932613055	36.0	36.0	36.0	32.0	36.0
32	34.67566453079075	36.0	36.0	36.0	32.0	36.0
33	34.65088950466639	36.0	36.0	36.0	32.0	36.0
34	34.64542376120779	36.0	36.0	36.0	32.0	36.0
35	34.62212439745423	36.0	36.0	36.0	32.0	36.0
36	34.58207827850517	36.0	36.0	36.0	32.0	36.0
37	34.584008846774275	36.0	36.0	36.0	32.0	36.0
38	34.53760746333671	36.0	36.0	36.0	32.0	36.0
39	34.54233357562254	36.0	36.0	36.0	32.0	36.0
40	34.528887015447495	36.0	36.0	36.0	32.0	36.0
41	34.51059292565692	36.0	36.0	36.0	32.0	36.0
42	34.45300216620298	36.0	36.0	36.0	32.0	36.0
43	34.44455400731188	36.0	36.0	36.0	32.0	36.0
44	34.39003304449536	36.0	36.0	36.0	32.0	36.0
45	34.3541340964407	36.0	36.0	36.0	32.0	36.0
46	34.334126418676405	36.0	36.0	36.0	32.0	36.0
47	34.31408470478735	36.0	36.0	36.0	32.0	36.0
48	34.26778444791278	36.0	36.0	36.0	32.0	36.0
49	34.25147623509406	36.0	36.0	36.0	32.0	36.0
50	33.58462116974961	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	4.0
18	1.0
19	14.0
20	17.0
21	71.0
22	206.0
23	579.0
24	1506.0
25	3712.0
26	7902.0
27	15532.0
28	27543.0
29	45770.0
30	72462.0
31	111742.0
32	176990.0
33	310726.0
34	662787.0
35	1618013.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.5031061470652	19.434979395449293	13.226547288679393	25.835367168806105
2	15.269495960800588	20.92542241084765	37.97888174852629	25.826199879825474
3	17.475794244945714	25.06114233818512	29.901573353624112	27.561490063245053
4	11.859429495640267	17.13869426298208	37.384755808804684	33.61712043257297
5	13.808324908847002	37.190651716517046	34.10982606558434	14.891197309051613
6	32.79492966301607	37.020155937865724	17.37849099482389	12.806423404294312
7	29.35794408581036	31.33616422440948	21.342856909092397	17.96303478068776
8	27.383076911496584	33.64130571738169	19.701909001147737	19.273708369973985
9	27.007201326897828	14.700439986542513	19.06116349669519	39.23119518986447
10	15.161752832625933	27.520285172228824	32.678460915636656	24.63950107950858
11	35.97117663865122	21.67286244136541	23.68547740737674	18.67048351260662
12	24.23583122652856	24.18055521464863	29.810952112122923	21.772661446699885
13	28.535844692146146	20.45100124134487	26.006354978814013	25.006799087694976
14	22.93063024616045	20.430014975939006	26.442906850706642	30.196447927193898
15	24.265842956324175	28.301918903412698	23.22324455079018	24.20899358947295
16	24.81263614561297	26.592020034350583	24.849748916071466	23.745594903964978
17	22.960188193649905	26.772300868968728	26.251678897857555	24.015832039523808
18	23.456580427409758	26.24398270829427	27.212312993095555	23.087123871200422
19	24.641892513263453	25.834335053575806	26.37151673808253	23.152255695078214
20	25.069365399053133	25.556674100115266	25.866044761709645	23.507915739121955
21	25.942334295617485	25.204274020913235	25.624980159230155	23.22841152423912
22	24.974382856755742	25.512907407486196	25.919574442878268	23.59313529287979
23	23.859373639773814	25.496194148499228	26.287178970256825	24.357253241470133
24	24.12749446256801	25.898883611819475	26.073090175050922	23.900531750561598
25	24.1980357574744	25.525792751435183	26.33187248427359	23.94429900681682
26	23.702456629095515	26.24192139480737	26.53932468800801	23.51629728808911
27	24.363130078693153	25.94124785789936	25.654968953450467	24.04065310995702
28	23.67385197648658	26.03717230244871	26.189817725524282	24.099157995540423
29	23.849783604188104	25.72982858519292	26.110223098452895	24.31016471216608
30	23.86325403963245	25.93474797380179	26.291713631525827	23.910284355039934
31	24.352097413102683	25.61319308140222	25.876206363965743	24.15850314152936
32	24.1000443792211	25.97739016142778	25.40402764341099	24.518537815940125
33	23.74051038081536	25.82416827145253	25.965064324530246	24.470257023201867
34	24.32164770845415	25.738277174347047	26.095535514411427	23.844539602787375
35	24.622270572287167	25.69980500698087	26.049208409507596	23.62871601122437
36	23.849031531678964	26.024566823986856	25.765381266896647	24.361020377437537
37	24.653685760124	25.81288852553231	25.473659957152144	24.05976575719155
38	24.018243319537927	25.83835319184451	25.859234494078446	24.284168994539115
39	24.499672552217366	25.653872802327903	25.39823116013391	24.448223485320817
40	24.81656102152438	25.821891715692818	25.81547707880054	23.54607018398226
41	23.95466642927929	25.982790287888925	26.26749150052823	23.795051782303556
42	24.79721107753614	26.162937401428298	25.604472172794324	23.43537934824123
43	24.120857434412446	25.835582258289385	25.807272967671118	24.23628733962705
44	24.034710778963422	25.947927286056288	25.500607913180506	24.516754021799784
45	24.129981649718758	26.17597091707879	25.64074619895213	24.053301234250323
46	24.116091739978643	25.801484446821178	25.471952830735162	24.610470982465017
47	24.279590000644735	25.872642829347704	25.827216801608373	24.020550368399192
48	24.673271331823717	26.237523797118456	25.03363587896909	24.055568992088734
49	24.225502289105012	26.123909815578873	25.395009296165256	24.25557859915086
50	24.24683353117116	26.26926300583237	25.31934690281532	24.164556560181154
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	6.0
1	11.0
2	16.0
3	75.0
4	134.0
5	170.0
6	206.0
7	209.0
8	212.0
9	297.0
10	382.0
11	617.0
12	852.0
13	1662.5
14	2473.0
15	3475.0
16	4477.0
17	5034.5
18	5592.0
19	6222.5
20	6853.0
21	8446.0
22	10039.0
23	12342.0
24	14645.0
25	20231.5
26	25818.0
27	33852.0
28	41886.0
29	48685.0
30	55484.0
31	62021.5
32	68559.0
33	79183.5
34	89808.0
35	103099.0
36	116390.0
37	130449.0
38	144508.0
39	153547.5
40	162587.0
41	173672.0
42	184757.0
43	187590.0
44	190423.0
45	200517.0
46	210611.0
47	219943.0
48	229275.0
49	229385.5
50	229496.0
51	221607.0
52	213718.0
53	208063.0
54	202408.0
55	197329.0
56	192250.0
57	183891.0
58	175532.0
59	158836.0
60	142140.0
61	124365.5
62	106591.0
63	91014.0
64	75437.0
65	63688.5
66	51940.0
67	43177.5
68	34415.0
69	30515.0
70	26615.0
71	20574.5
72	14534.0
73	12158.5
74	9783.0
75	7525.5
76	5268.0
77	4567.0
78	3866.0
79	3111.0
80	2356.0
81	1846.5
82	1337.0
83	1147.0
84	957.0
85	677.5
86	398.0
87	298.0
88	198.0
89	154.0
90	110.0
91	88.5
92	67.0
93	59.0
94	51.0
95	53.0
96	55.0
97	43.5
98	32.0
99	31.0
100	30.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.006021775919899907
2	6.872679039016199E-4
3	6.545408608586856E-5
4	0.0
5	0.0
6	3.272704304293428E-5
7	6.545408608586856E-5
8	0.0
9	9.490842482450942E-4
10	6.545408608586856E-4
11	0.0
12	6.545408608586856E-5
13	6.545408608586856E-4
14	2.945433873864085E-4
15	0.004385423767753194
16	8.18176076073357E-4
17	3.272704304293428E-5
18	2.6181634434347426E-4
19	0.0
20	6.218138178157513E-4
21	0.0
22	1.9636225825760565E-4
23	6.218138178157513E-4
24	2.945433873864085E-4
25	0.0015708980660608452
26	0.004254515595581457
27	0.005399962102084157
28	0.004254515595581457
29	0.0021272577977907285
30	0.0033054313473363625
31	0.007134495383359673
32	0.003043615002992888
33	0.004352696724710259
34	0.002781798658649414
35	0.002716344572563545
36	0.007756309201175425
37	0.0037636099499374423
38	0.006938133125102068
39	0.004189061509495588
40	0.0021272577977907285
41	0.003043615002992888
42	0.0019636225825760568
43	0.001407262850846174
44	0.0013745358078032397
45	0.0012436276356315027
46	9.163572052021599E-4
47	0.0019636225825760568
48	0.0019636225825760568
49	5.236326886869485E-4
50	0.0014399898938891084
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	3055577.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.174171542045805
#Duplication Level	Percentage of deduplicated	Percentage of total
1	63.868292491182046	25.65855738638303
2	15.82892697490884	12.718280552330077
3	7.422144152098976	8.945354771286492
4	4.009496581664846	6.44312813876199
5	2.3979785112780485	4.816840003311197
6	1.569688159315398	3.783655282791295
7	1.0585710169385443	2.976904953674887
8	0.7430415170314181	2.388086189446571
9	0.5374646753310263	1.943297825808474
>10	2.363267019995457	15.80559129504401
>50	0.10652199854473761	2.9683448013267726
>100	0.08095178857896372	6.633437168999966
>500	0.009730290196047063	2.6443007697202487
>1k	0.003924822935908839	2.274220861115067
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.272704304293428E-5
2	0.0	0.0	0.0	0.0	3.272704304293428E-5
3	0.0	0.0	0.0	0.0	3.272704304293428E-5
4	0.0	0.0	0.0	0.0	3.272704304293428E-5
5	0.0	0.0	0.0	0.0	3.272704304293428E-5
6	0.0	0.0	0.0	0.0	1.3090817217173713E-4
7	0.0	0.0	0.0	0.0	1.3090817217173713E-4
8	0.0	0.0	0.0	0.0	1.3090817217173713E-4
9	0.0	0.0	0.0	0.0	1.636352152146714E-4
10	0.0	0.0	0.0	0.0	1.636352152146714E-4
11	0.0	0.0	0.0	0.0	1.636352152146714E-4
12	0.0	0.0	0.0	0.0	1.636352152146714E-4
13	0.0	0.0	0.0	0.0	1.636352152146714E-4
14	0.0	0.0	0.0	0.0	1.636352152146714E-4
15	0.0	0.0	0.0	0.0	2.2908930130053997E-4
16	0.0	0.0	0.0	0.0	2.2908930130053997E-4
17	0.0	0.0	0.0	0.0	2.945433873864085E-4
18	0.0	0.0	0.0	0.0	2.945433873864085E-4
19	0.0	0.0	0.0	0.0	2.945433873864085E-4
20	0.0	0.0	0.0	0.0	2.945433873864085E-4
21	0.0	0.0	0.0	3.272704304293428E-5	2.945433873864085E-4
22	0.0	0.0	0.0	3.272704304293428E-5	2.945433873864085E-4
23	0.0	0.0	0.0	3.272704304293428E-5	3.599974734722771E-4
24	0.0	0.0	0.0	3.272704304293428E-5	3.599974734722771E-4
25	0.0	0.0	0.0	3.272704304293428E-5	3.9272451651521136E-4
26	0.0	0.0	0.0	3.272704304293428E-5	3.9272451651521136E-4
27	0.0	0.0	0.0	3.272704304293428E-5	3.9272451651521136E-4
28	0.0	0.0	0.0	9.818112912880284E-5	3.9272451651521136E-4
29	0.0	0.0	0.0	1.9636225825760568E-4	3.9272451651521136E-4
30	0.0	0.0	0.0	3.9272451651521136E-4	3.9272451651521136E-4
31	0.0	0.0	0.0	0.0011454465065026999	3.9272451651521136E-4
32	0.0	0.0	0.0	0.002094530754747794	3.9272451651521136E-4
33	0.0	0.0	0.0	0.0030763420460358224	3.9272451651521136E-4
34	0.0	0.0	0.0	0.004647240112096667	4.909056456440142E-4
35	0.0	0.0	0.0	0.006545408608586856	4.909056456440142E-4
36	0.0	0.0	0.0	0.010898105333297116	4.909056456440142E-4
37	0.0	0.0	0.0	0.01688715421015409	4.909056456440142E-4
38	0.0	0.0	0.0	0.02791616771562294	4.909056456440142E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	4685	0.0	27.001007	1
GGTATCA	1855	0.0	22.65224	1
TACGCGG	55	0.00448436	20.00061	35
CGTCTTA	465	0.0	18.92438	15
TCAACGC	6835	0.0	18.377989	4
AACGCAG	6865	0.0	18.169498	6
TATCAAC	7060	0.0	17.979542	2
GTAAACG	540	0.0	17.92706	27
CAACGCA	6980	0.0	17.901659	5
ATCAACG	7015	0.0	17.875061	3
CGCGGGA	560	0.0	17.285395	44
AATACCG	115	4.2792926E-6	17.216509	5
TAAACGC	570	0.0	16.98353	28
CGCAATA	390	0.0	16.923594	36
ATACCGT	835	0.0	16.86141	6
TAGGACG	775	0.0	16.179813	4
ACCGTCG	825	0.0	15.99944	8
CGTCGTA	815	0.0	15.926344	10
TACCGTC	830	0.0	15.903058	7
CCGTCGT	850	0.0	15.788199	9
>>END_MODULE