FastQCFastQC Report
Thu 2 Feb 2017
SRR4063872_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063872_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2320939
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT46700.20121166476154695No Hit
TATCAACGCAGAGTACTTTTTTTTT32590.14041730523723372No Hit
GTCCTAAAGTGTGTATTTCTCATTT29200.12581114798794799No Hit
GTCCTACAGTGGACATTTCTAAATT23840.10271704685043426No Hit
CTTTAGGACGTGAAATATGGCGAGG23820.10263087483126442No Hit
CTGTAGGACGTGGAATATGGCAAGA23250.10017497228492432No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG3450.012.6630881
GCGTTAT1353.7289283E-1011.9595831
GAACCGA802.8710178E-511.8736936
GTATCAA33600.011.7020511
CGTGCGG658.024734E-411.692533510
GTCCTAG2700.011.6078311
ACCGTCG3200.011.5785998
CGTCGTA3350.011.34350210
ATACCGT3450.011.2886126
CGAGCCG3300.011.22966915
GTCCTAT4750.011.1968351
GCCTAGT855.3405987E-511.1733121
CGATAAC1201.2758392E-711.08354810
CCGTCGT3450.011.0149429
TTAACGA1900.011.00092412
TTAGGAC9350.010.968983
GTATTAT1752.0008883E-1110.8540751
CGTTATT1502.1500455E-910.7617692
GCTCGTA1158.8170964E-710.7395699
TACCGTC3450.010.7386437
Produced by FastQC (version 0.11.5)